Restriction map for
pFN21AB0488
(Circular) (Six-base) MAPSORT of: pFN21AB0488.seq Check: 198 from: 1 to: 7801
pFN21AB0488 7801 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3259 3386 4924 4957 3259
Size: 127 1538 33 6103
Fragments arranged by size:
6103 1538 127 33
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5611 278
Size: 53 83 186 936 4075 2468
Fragments arranged by size:
4075 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5144 1937
Size: 3207 4594
Acc65I G'GTAC_C
Cuts at: 5122 5122
Size: 7801
AclI AA'CG_TT
Cuts at: 1563 5928 6301 1563
Size: 4365 373 3063
Fragments arranged by size:
4365 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3628 4400 5864 7248 7516 1632
Size: 1996 772 1464 1384 268 1917
Fragments arranged by size:
1996 1917 1464 1384 772 268
AfeI AGC'GCT
Cuts at: 2360 2555 2360
Size: 195 7606
AflII C'TTAA_G
Cuts at: 820 1017 3680 820
Size: 197 2663 4941
Fragments arranged by size:
4941 2663 197
AflIII A'CryG_T
Cuts at: 1203 6700 7677 1203
Size: 5497 977 1327
Fragments arranged by size:
5497 1327 977
AgeI A'CCGG_T
Cuts at: 6632 6632
Size: 7801
AhdI GACnn_n'nnGTC
Cuts at: 3886 6530 3886
Size: 2644 5157
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3287 1184
Size: 2103 5698
AlwNI CAG_nnn'CTG
Cuts at: 2473 2527 2872 3007 3019 3073 4462 4679 5079
Size: 54 345 135 12 54 1389 217 400
Cuts at: 5079 5544 7116 2473
Size: 465 1572 3158
Fragments arranged by size:
3158 1572 1389 465 400 345 217 135
54 54 12
ApaI G_GGCC'C
Cuts at: 2774 2935 3367 3836 2774
Size: 161 432 469 6739
Fragments arranged by size:
6739 469 432 161
ApaLI G'TGCA_C
Cuts at: 2748 5858 7014 2748
Size: 3110 1156 3535
Fragments arranged by size:
3535 3110 1156
ApoI r'AATT_y
Cuts at: 1493 5110 5336 5488 7597 1493
Size: 3617 226 152 2109 1697
Fragments arranged by size:
3617 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6355 160
Size: 872 5323 1606
Fragments arranged by size:
5323 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2481 2495 2935 3762 4248 4782
Size: 73 113 425 14 440 827 486 534
Cuts at: 4782 5126 1870
Size: 344 4545
Fragments arranged by size:
4545 827 534 486 440 425 344 113
73 14
BamHI G'GATC_C
Cuts at: 3216 4725 5131 5498 3216
Size: 1509 406 367 5519
Fragments arranged by size:
5519 1509 406 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2241 3329 3374 4112 4286
Size: 325 217 627 454 1088 45 738 174
Cuts at: 4286 4356 4432 4570 4969 5122 6577 618
Size: 70 76 138 399 153 1455 1842
Fragments arranged by size:
1842 1455 1088 738 627 454 399 325
217 174 153 138 76 70 45
BanII G_rGCy'C
Cuts at: 1391 2774 2935 2942 3367 3824 3836 4122 4312
Size: 1383 161 7 425 457 12 286 190
Cuts at: 4312 4393 4473 4687 5120 1391
Size: 81 80 214 433 4072
Fragments arranged by size:
4072 1383 457 433 425 286 214 190
161 81 80 12 7
BbeI G_GCGC'C
Cuts at: 2245 4436 4574 2245
Size: 2191 138 5472
Fragments arranged by size:
5472 2191 138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3093 3624 928
Size: 971 1194 531 5105
Fragments arranged by size:
5105 1194 971 531
BbvCI CC'TCA_GC
Cuts at: 3422 4672 3422
Size: 1250 6551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5992 6026 1903
Size: 34 50 34 3971 34 3678
Fragments arranged by size:
3971 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5693 6903 5693
Size: 1210 6591
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7801
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3327 4691 4887
Size: 107 122 71 631 605 1655 1364 196
Cuts at: 4887 4891 6412 136
Size: 4 1521 1525
Fragments arranged by size:
1655 1525 1521 1364 631 605 196 122
107 71 4
BglII A'GATC_T
Cuts at: 4812 7796 4812
Size: 2984 4817
BlpI GC'TnA_GC
Cuts at: 2201 4695 5218 2201
Size: 2494 523 4784
Fragments arranged by size:
4784 2494 523
Bme1580I G_kGCm'C
Cuts at: 1790 2752 2774 2935 3332 3367 3836 4289 4296
Size: 962 22 161 397 35 469 453 7
Cuts at: 4296 4634 4667 4974 5862 7018 1790
Size: 338 33 307 888 1156 2573
Fragments arranged by size:
2573 1156 962 888 469 453 397 338
307 161 35 33 22 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7801
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2587 3489 3576
Size: 416 53 83 399 24 1170 902 87
Cuts at: 3576 5532 6490 442
Size: 1956 958 1753
Fragments arranged by size:
1956 1753 1170 958 902 416 399 87
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7801
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2683 3469 2683
Size: 786 7015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2537 2615 2756 3188 3302 3344 3365
Size: 1085 213 78 141 432 114 42 21
Cuts at: 3365 3995 4333 4487 4701 4790 6461 1239
Size: 630 338 154 214 89 1671 2579
Fragments arranged by size:
2579 1671 1085 630 432 338 214 213
154 141 114 89 78 42 21
Bpu10I CC'TnA_GC
Cuts at: 2547 3422 3440 3507 4672 2547
Size: 875 18 67 1165 5676
Fragments arranged by size:
5676 1165 875 67 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2699 3083 3368 5926 6791 7089 2594
Size: 105 384 285 2558 865 298 3306
Fragments arranged by size:
3306 2558 865 384 298 285 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3183 3798 6464 882
Size: 617 1684 615 2666 2219
Fragments arranged by size:
2666 2219 1684 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 4834 5592 493
Size: 711 3630 758 2702
Fragments arranged by size:
3630 2702 758 711
BsaBI GATnn'nnATC
Cuts at: 2252 7795 2252
Size: 5543 2258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2242 2879 4433
Size: 53 83 186 936 570 139 637 1554
Cuts at: 4433 4571 5608 5990 6625 275
Size: 138 1037 382 635 1451
Fragments arranged by size:
1554 1451 1037 936 637 635 570 382
186 139 138 83 53
BsaWI w'CCGG_w
Cuts at: 3213 6234 6632 6906 7053 7464 3213
Size: 3021 398 274 147 411 3550
Fragments arranged by size:
3550 3021 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2729 2759 3029 3059 3968
Size: 30 165 30 1326 30 270 30 909
Cuts at: 3968 3998 4646 4676 1178
Size: 30 648 30 4303
Fragments arranged by size:
4303 1326 909 648 270 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2087 2204 2207 2396 2513 2516
Size: 865 3 33 117 3 189 117 3
Cuts at: 2516 2672 2804 3068 3185 3388 5012 1186
Size: 156 132 264 117 203 1624 3975
Fragments arranged by size:
3975 1624 865 264 203 189 156 132
117 117 117 33 3 3 3
BseYI C'CCAG_C
Cuts at: 2355 4002 4311 4604 4633 4774 4876 5084 7004
Size: 1647 309 293 29 141 102 208 1920
Cuts at: 7004 7500 2355
Size: 496 2656
Fragments arranged by size:
2656 1920 1647 496 309 293 208 141
102 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3050 4228 4497 4823 4985 1163
Size: 573 1314 1178 269 326 162 3979
Fragments arranged by size:
3979 1314 1178 573 326 269 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2927 4520 4886 5023 5251 6012
Size: 275 186 926 1593 366 137 228 761
Cuts at: 6012 7040 1540
Size: 1028 2301
Fragments arranged by size:
2301 1593 1028 926 761 366 275 228
186 137
BsiHKAI G_wGCw'C
Cuts at: 2207 2496 2752 3416 4783 4941 5862 5947 7018
Size: 289 256 664 1367 158 921 85 1071
Cuts at: 7018 2207
Size: 2990
Fragments arranged by size:
2990 1367 1071 921 664 289 256 158
85
BsmI GAATG_Cn'
Cuts at: 5312 5405 5312
Size: 93 7708
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 2496 2752 2774 2935 2942 3332
Size: 399 417 289 256 22 161 7 390
Cuts at: 3332 3367 3416 3824 3836 4122 4289 4296 4312
Size: 35 49 408 12 286 167 7 16
Cuts at: 4312 4393 4473 4634 4667 4687 4783 4941 4974
Size: 81 80 161 33 20 96 158 33
Cuts at: 4974 5120 5862 5947 7018 1391
Size: 146 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 417 408 399 390 289
286 256 167 161 161 158 146 96
85 81 80 49 35 33 33 22
20 16 12 7 7
BspEI T'CCGG_A
Cuts at: 7464 7464
Size: 7801
BspHI T'CATG_A
Cuts at: 5690 7420 5690
Size: 1730 6071
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2700 3081 3099 3259 3386 4599 4608 4924
Size: 1856 381 18 160 127 1213 9 316
Cuts at: 4924 4957 5156 844
Size: 33 199 3489
Fragments arranged by size:
3489 1856 1213 381 316 199 160 127
33 18 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2506 3292 4489 5688 1394
Size: 429 683 786 1197 1199 3507
Fragments arranged by size:
3507 1199 1197 786 683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6296 6470 65
Size: 1183 5048 174 1396
Fragments arranged by size:
5048 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2922 3587 3603 4353 4881 6445
Size: 110 241 871 665 16 750 528 1564
Cuts at: 6445 6632 1700
Size: 187 2869
Fragments arranged by size:
2869 1564 871 750 665 528 241 187
110 16
BsrGI T'GTAC_A
Cuts at: 96 3132 96
Size: 3036 4765
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7801
BssSI C'ACGA_G
Cuts at: 4079 5048 5861 6873 4079
Size: 969 813 1012 5007
Fragments arranged by size:
5007 1012 969 813
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 2527 3028 4765 5079 794
Size: 1679 54 501 1737 314 3516
Fragments arranged by size:
3516 1737 1679 501 314 54
BstBI TT'CG_AA
Cuts at: 2758 6605 2758
Size: 3847 3954
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7801
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 3157 4426 2641
Size: 516 1269 6016
Fragments arranged by size:
6016 1269 516
BstYI r'GATC_y
Cuts at: 1972 3216 3638 4162 4194 4725 4812 5131 5498
Size: 1244 422 524 32 531 87 319 367
Cuts at: 5498 5883 5900 7352 7438 7450 7796 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1244 531 524 422 385 367
346 319 87 86 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 2391 2391
Size: 7801
BtgI C'CryG_G
Cuts at: 513 1065 2266 4425 4545 4921 513
Size: 552 1201 2159 120 376 3393
Fragments arranged by size:
3393 2159 1201 552 376 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7062
Fragments arranged by size:
7062 683 56
BtsI GCAGTG_nn'
Cuts at: 2750 2882 3050 4759 4922 5098 5317 6111 6131
Size: 132 168 1709 163 176 219 794 20
Cuts at: 6131 2750
Size: 4420
Fragments arranged by size:
4420 1709 794 219 176 168 163 132
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7766
DraI TTT'AAA
Cuts at: 5105 5105
Size: 7801
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6808 809
Size: 507 5492 1802
Fragments arranged by size:
5492 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2924 3147
Size: 54 1425 50 275 18 306 788 223
Cuts at: 3147 3814 4517 4883 5004 5020 5248 6137 7766
Size: 667 703 366 121 16 228 889 1629
Cuts at: 7766 8
Size: 43
Fragments arranged by size:
1629 1425 889 788 703 667 366 306
275 228 223 121 54 50 43 18
16
EagI C'GGCC_G
Cuts at: 1537 1812 2924 4517 4883 5020 5248 1537
Size: 275 1112 1593 366 137 228 4090
Fragments arranged by size:
4090 1593 1112 366 275 228 137
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 3709 4379 5731 1353
Size: 1383 973 670 1352 3423
Fragments arranged by size:
3423 1383 1352 973 670
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 2084 3302 3590 3919 6388
Size: 1064 138 444 51 1218 288 329 2469
Cuts at: 6388 6762 6908 387
Size: 374 146 1280
Fragments arranged by size:
2469 1280 1218 1064 444 374 329 288
146 138 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2537 2615 2756 3188 3302 3344
Size: 393 692 213 78 141 432 114 42
Cuts at: 3344 3365 3628 3995 4333 4400 4487 4701 4790
Size: 21 263 367 338 67 87 214 89
Cuts at: 4790 5864 6461 7248 7516 1239
Size: 1074 597 787 268 1524
Fragments arranged by size:
1524 1074 787 692 597 432 393 367
338 268 263 214 213 141 114 89
87 78 67 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 2909 3462 4713 2378
Size: 531 553 1251 5466
Fragments arranged by size:
5466 1251 553 531
EcoO109I rG'GnC_Cy
Cuts at: 2387 2395 2770 2902 2931 2932 3277 3503 3832
Size: 8 375 132 29 1 345 226 329
Cuts at: 3832 4084 4273 4364 2387
Size: 252 189 91 5824
Fragments arranged by size:
5824 375 345 329 252 226 189 132
91 29 8 1
EcoRI G'AATT_C
Cuts at: 5110 5488 5110
Size: 378 7423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3658 4214 3658
Size: 556 7245
FseI GG_CCGG'CC
Cuts at: 4885 4885
Size: 7801
FspI TGC'GCA
Cuts at: 3905 6307 3905
Size: 2402 5399
HaeII r_GCGC'y
Cuts at: 1678 2245 2362 2557 4436 4574 4679 6948 1678
Size: 567 117 195 1879 138 105 2269 2531
Fragments arranged by size:
2531 2269 1879 567 195 138 117 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 3851 3872 3883
Size: 32 135 32 1256 32 991 21 11
Cuts at: 3883 3904 4147 4179 6442 6474 6516 6548 1373
Size: 21 243 32 2263 32 42 32 2626
Fragments arranged by size:
2626 2263 1256 991 243 135 42 32
32 32 32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2230 2561 4255 5145 5391 669
Size: 988 281 292 331 1694 890 246 3079
Fragments arranged by size:
3079 1694 988 890 331 292 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7801
HpaI GTT'AAC
Cuts at: 5391 5391
Size: 7801
KasI G'GCGC_C
Cuts at: 2241 4432 4570 2241
Size: 2191 138 5472
Fragments arranged by size:
5472 2191 138
KpnI G_GTAC'C
Cuts at: 5126 5126
Size: 7801
MfeI C'AATT_G
Cuts at: 5400 5400
Size: 7801
MluI A'CGCG_T
Cuts at: 7677 7677
Size: 7801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3441 6915 7099 1305
Size: 2136 3474 184 2007
Fragments arranged by size:
3474 2136 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3149 5006 10
Size: 54 1425 343 1317 1857 2805
Fragments arranged by size:
2805 1857 1425 1317 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4832 5759 6118 6277 518
Size: 648 3666 927 359 159 2042
Fragments arranged by size:
3666 2042 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2455 2461 2524 2698 2869 3355 3361 3491
Size: 849 6 63 174 171 486 6 130
Cuts at: 3491 4402 4459 4637 5217 5894 7042 7287 1606
Size: 911 57 178 580 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 849 677 580 486 245
178 174 171 130 63 57 6 6
NaeI GCC'GGC
Cuts at: 2053 2924 3589 3605 4355 4883 2053
Size: 871 665 16 750 528 4971
Fragments arranged by size:
4971 871 750 665 528 16
NarI GG'CG_CC
Cuts at: 2242 4433 4571 2242
Size: 2191 138 5472
Fragments arranged by size:
5472 2191 138
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7249
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7801
NgoMIV G'CCGG_C
Cuts at: 2051 2922 3587 3603 4353 4881 2051
Size: 871 665 16 750 528 4971
Fragments arranged by size:
4971 871 750 665 528 16
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7801
NotI GC'GGCC_GC
Cuts at: 4517 5020 5248 4517
Size: 503 228 7070
Fragments arranged by size:
7070 503 228
NspI r_CATG'y
Cuts at: 1132 1229 3026 3655 5159 6704 1132
Size: 97 1797 629 1504 1545 2229
Fragments arranged by size:
2229 1797 1545 1504 629 97
PasI CC'CwG_GG
Cuts at: 1348 2780 3367 3827 4709 1348
Size: 1432 587 460 882 4440
Fragments arranged by size:
4440 1432 882 587 460
PciI A'CATG_T
Cuts at: 6700 6700
Size: 7801
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7801
PfoI T'CCnGG_A
Cuts at: 2292 4737 4857 2292
Size: 2445 120 5236
Fragments arranged by size:
5236 2445 120
PmeI GTTT'AAAC
Cuts at: 5105 5105
Size: 7801
PmlI CAC'GTG
Cuts at: 4834 4834
Size: 7801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5870 5902 7383 7415 5870
Size: 32 1481 32 6256
Fragments arranged by size:
6256 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3503 4273 3503
Size: 770 7031
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7801
PsiI TTA'TAA
Cuts at: 5371 5371
Size: 7801
PspOMI G'GGCC_C
Cuts at: 2770 2931 3363 3832 2770
Size: 161 432 469 6739
Fragments arranged by size:
6739 469 432 161
PspXI vC'TCGA_Gb
Cuts at: 2495 2495
Size: 7801
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4799 4831 4799
Size: 32 7769
PstI C_TGCA'G
Cuts at: 830 2460 2478 2697 2910 3045 3078 3117 3360
Size: 1630 18 219 213 135 33 39 243
Cuts at: 3360 4441 5034 5153 830
Size: 1081 593 119 3478
Fragments arranged by size:
3478 1630 1081 593 243 219 213 135
119 39 33 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7801
PvuII CAG'CTG
Cuts at: 2455 2698 3355 3491 4402 4459 2455
Size: 243 657 136 911 57 5797
Fragments arranged by size:
5797 911 657 243 136 57
SalI G'TCGA_C
Cuts at: 1936 5143 1936
Size: 3207 4594
SbfI CC_TGCA'GG
Cuts at: 3078 4441 5034 5153 3078
Size: 1363 593 119 5726
Fragments arranged by size:
5726 1363 593 119
ScaI AGT'ACT
Cuts at: 3230 6049 7761 3230
Size: 2819 1712 3270
Fragments arranged by size:
3270 2819 1712
SexAI A'CCwGG_T
Cuts at: 4108 4108
Size: 7801
SfcI C'TryA_G
Cuts at: 826 1045 2456 2474 2693 2906 3041 3074 3113
Size: 219 1411 18 219 213 135 33 39
Cuts at: 3113 3356 4437 4504 5030 5149 6284 6965 7156
Size: 243 1081 67 526 119 1135 681 191
Cuts at: 7156 826
Size: 1471
Fragments arranged by size:
1471 1411 1135 1081 681 526 243 219
219 213 191 135 119 67 39 33
18
SfoI GGC'GCC
Cuts at: 2243 4434 4572 2243
Size: 2191 138 5472
Fragments arranged by size:
5472 2191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7801
SmaI CCC'GGG
Cuts at: 1872 2483 2937 4250 5128 1872
Size: 611 454 1313 878 4545
Fragments arranged by size:
4545 1313 878 611 454
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 2609 2714 3098 3383 3680
Size: 197 926 552 114 105 384 285 297
Cuts at: 3680 5905 6806 7068 820
Size: 2225 901 262 1553
Fragments arranged by size:
2225 1553 926 901 552 384 297 285
262 197 114 105
SnaBI TAC'GTA
Cuts at: 493 5592 493
Size: 5099 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7801
SphI G_CATG'C
Cuts at: 1132 3026 5159 1132
Size: 1894 2133 3774
Fragments arranged by size:
3774 2133 1894
SspI AAT'ATT
Cuts at: 5 52 5725 5
Size: 47 5673 2081
Fragments arranged by size:
5673 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 3389 3753 4064 1517
Size: 771 1101 364 311 5254
Fragments arranged by size:
5254 1101 771 364 311
StyI C'CwwG_G
Cuts at: 513 1065 3333 4689 5007 5240 513
Size: 552 2268 1356 318 233 3074
Fragments arranged by size:
3074 2268 1356 552 318 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4766 4951 5044 5828 5845 5998 6183
Size: 1255 3186 185 93 784 17 153 185
Cuts at: 6183 6628 325
Size: 445 1498
Fragments arranged by size:
3186 1498 1255 784 445 185 185 153
93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2674 3132 3228
Size: 274 80 33 51 1443 697 458 96
Cuts at: 3228 6047 7759 96
Size: 2819 1712 138
Fragments arranged by size:
2819 1712 1443 697 458 274 138 96
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 2729 2761 3029 3061 3968 4000 4646
Size: 32 628 32 268 32 907 32 646
Cuts at: 4646 4678 7761 7793 2069
Size: 32 3083 32 2077
Fragments arranged by size:
3083 2077 907 646 628 268 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2840 2878 1316
Size: 1524 38 6239
Fragments arranged by size:
6239 1524 38
XbaI T'CTAG_A
Cuts at: 5137 5137
Size: 7801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3340 4612 4745 3340
Size: 1272 133 6396
Fragments arranged by size:
6396 1272 133
XhoI C'TCGA_G
Cuts at: 1943 2495 1943
Size: 552 7249
XmaI C'CCGG_G
Cuts at: 1870 2481 2935 4248 5126 1870
Size: 611 454 1313 878 4545
Fragments arranged by size:
4545 1313 878 611 454
XmnI GAAnn'nnTTC
Cuts at: 5930 5930
Size: 7801
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5609 276
Size: 53 83 186 936 4075 2468
Fragments arranged by size:
4075 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoRV FspAI NruI NsiI PacI RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI