Restriction map for
pFN21AB0487
(Circular) (Six-base) MAPSORT of: pFN21AB0487.seq Check: 8063 from: 1 to: 6292
pFN21AB0487 6292 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2478 3635 1937
Size: 541 1157 4594
Fragments arranged by size:
4594 1157 541
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 4355 5739 6007 1632
Size: 620 2103 1384 268 1917
Fragments arranged by size:
2103 1917 1384 620 268
AfeI AGC'GCT
Cuts at: 2467 2467
Size: 6292
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 2783 3270 1166
Size: 1617 487 4188
Fragments arranged by size:
4188 1617 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2783 1184
Size: 1599 4693
AlwNI CAG_nnn'CTG
Cuts at: 2273 4035 5607 2273
Size: 1762 1572 2958
Fragments arranged by size:
2958 1762 1572
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 2096 2150 2358 2923 3601 3827 3979 6088
Size: 603 54 208 565 678 226 152 2109
Cuts at: 6088 1493
Size: 1697
Fragments arranged by size:
2109 1697 678 603 565 226 208 152
54
AseI AT'TA_AT
Cuts at: 160 1032 2381 4846 160
Size: 872 1349 2465 1606
Fragments arranged by size:
2465 1606 1349 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3617 1870
Size: 73 1674 4545
Fragments arranged by size:
4545 1674 73
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3613 5068 618
Size: 325 217 627 1826 1455 1842
Fragments arranged by size:
1842 1826 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2989 3611 1391
Size: 1598 622 4072
Fragments arranged by size:
4072 1598 622
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3207 3287 928
Size: 971 1308 80 3933
Fragments arranged by size:
3933 1308 971 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2152 2333 2998 4184 5394 2152
Size: 181 665 1186 1210 3050
Fragments arranged by size:
3050 1210 1186 665 181
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4903 136
Size: 107 122 71 631 605 3231 1525
Fragments arranged by size:
3231 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 4353 5509 1790
Size: 2563 1156 2573
Fragments arranged by size:
2573 2563 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4023 4981 442
Size: 416 53 83 399 24 2606 958 1753
Fragments arranged by size:
2606 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 4952 1239
Size: 852 2861 2579
Fragments arranged by size:
2861 2579 852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4417 5282 5580 4417
Size: 865 298 5129
Fragments arranged by size:
5129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3026 4955 882
Size: 617 1527 1929 2219
Fragments arranged by size:
2219 1929 1527 617
BsaAI yAC'GTr
Cuts at: 493 1204 2402 4083 493
Size: 711 1198 1681 2702
Fragments arranged by size:
2702 1681 1198 711
BsaBI GATnn'nnATC
Cuts at: 2792 6286 2792
Size: 3494 2798
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4099 4481 5116 275
Size: 53 83 186 936 2566 382 635 1451
Fragments arranged by size:
2566 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6067
Fragments arranged by size:
6067 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6292
BseYI C'CCAG_C
Cuts at: 2811 5495 5991 2811
Size: 2684 496 3112
Fragments arranged by size:
3112 2684 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5719
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2893 4353 4438 5509 2893
Size: 1460 85 1071 3676
Fragments arranged by size:
3676 1460 1071 85
BsmI GAATG_Cn'
Cuts at: 2180 3090 3803 3896 2180
Size: 910 713 93 4576
Fragments arranged by size:
4576 910 713 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2893 2989 3611 4353 4438 5509 1391
Size: 399 1103 96 622 742 85 1071 2174
Fragments arranged by size:
2174 1103 1071 742 622 399 96 85
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 4181 5911 4181
Size: 1730 4562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 3647 844
Size: 2056 747 3489
Fragments arranged by size:
3489 2056 747
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2847 3156 4787 4961 65
Size: 1183 1599 309 1631 174 1396
Fragments arranged by size:
1631 1599 1396 1183 309 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4936 5123 1700
Size: 110 3126 187 2869
Fragments arranged by size:
3126 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 3041 4352 5364 3041
Size: 1311 1012 3969
Fragments arranged by size:
3969 1311 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287
Size: 1650 367 385 17 1452 86 12 346
Cuts at: 6287 1972
Size: 1977
Fragments arranged by size:
1977 1650 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5740
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2563 487
Size: 683 56 1337 4216
Fragments arranged by size:
4216 1337 683 56
BtsI GCAGTG_nn'
Cuts at: 3808 4602 4622 3808
Size: 794 20 5478
Fragments arranged by size:
5478 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 2922 3596 2922
Size: 674 5618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2844 2928 3739
Size: 54 1425 50 275 18 1014 84 811
Cuts at: 3739 4628 6257 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1014 889 811 275 84 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3408 4222 1353
Size: 2055 814 3423
Fragments arranged by size:
3423 2055 814
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2252 4355 4952 5739 6007 1239
Size: 393 459 161 2103 597 787 268 1524
Fragments arranged by size:
2103 1524 787 597 459 393 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 2583 2583
Size: 6292
EcoRI G'AATT_C
Cuts at: 2358 3601 3979 2358
Size: 1243 378 4671
Fragments arranged by size:
4671 1243 378
EcoRV GAT'ATC
Cuts at: 2724 2724
Size: 6292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3521 3521
Size: 6292
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2469 5439 1678
Size: 791 2970 2531
Fragments arranged by size:
2970 2531 791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3274 3306 4933 4965 5007
Size: 32 135 32 1702 32 1627 32 42
Cuts at: 5007 5039 1373
Size: 32 2626
Fragments arranged by size:
2626 1702 1627 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2666 748
Size: 1918 4374
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3537 3891 3537
Size: 354 5938
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2866 3338 5406 5590 1305
Size: 1561 472 2068 184 2007
Fragments arranged by size:
2068 2007 1561 472 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2846 2930 10
Size: 54 1425 343 1014 84 3372
Fragments arranged by size:
3372 1425 1014 343 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2783 3270 4250 4609 4768 518
Size: 648 843 774 487 980 359 159 2042
Fragments arranged by size:
2042 980 843 774 648 487 359 159
MspA1I CmG'CkG
Cuts at: 1606 2218 2263 2714 3708 4385 5533 5778 1606
Size: 612 45 451 994 677 1148 245 2120
Fragments arranged by size:
2120 1148 994 677 612 451 245 45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5740
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 2939 3650 5195 1132
Size: 97 1710 711 1545 2229
Fragments arranged by size:
2229 1710 1545 711 97
PasI CC'CwG_GG
Cuts at: 1348 2387 1348
Size: 1039 5253
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6292
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PmlI CAC'GTG
Cuts at: 2402 2402
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 4361 4393 5874 5906 2642
Size: 32 1687 32 1481 32 3028
Fragments arranged by size:
3028 1687 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PstI C_TGCA'G
Cuts at: 830 2719 3644 830
Size: 1889 925 3478
Fragments arranged by size:
3478 1889 925
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2218 2714 2218
Size: 496 5796
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 2715 3640 4775 5456 5647 826
Size: 219 1670 925 1135 681 191 1471
Fragments arranged by size:
1670 1471 1135 925 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4396 5297 5559 820
Size: 197 926 2453 901 262 1553
Fragments arranged by size:
2453 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 2939 3650 1132
Size: 1807 711 3774
Fragments arranged by size:
3774 1807 711
SspI AAT'ATT
Cuts at: 5 52 2764 4216 5
Size: 47 2712 1452 2081
Fragments arranged by size:
2712 2081 1452 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 3731 513
Size: 552 2666 3074
Fragments arranged by size:
3074 2666 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 2869 4319 4336 4489 4674 5119
Size: 1255 1215 74 1450 17 153 185 445
Cuts at: 5119 325
Size: 1498
Fragments arranged by size:
1498 1450 1255 1215 445 185 153 74
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4538 6250 96
Size: 274 80 33 51 1443 2561 1712 138
Fragments arranged by size:
2561 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 2996 3628 2996
Size: 632 5660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3103 3103
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 2362 3076 4421 2362
Size: 714 1345 4233
Fragments arranged by size:
4233 1345 714
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI