Restriction map for
pFN21AB0485
(Circular) (Six-base) MAPSORT of: pFN21AB0485.seq Check: 7032 from: 1 to: 6289
pFN21AB0485 6289 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2999 3294 2999
Size: 295 5994
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3632 1937
Size: 1695 4594
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4352 5736 6004 1632
Size: 2720 1384 268 1917
Fragments arranged by size:
2720 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 5188 6165 1203
Size: 3985 977 1327
Fragments arranged by size:
3985 1327 977
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 2676 5018 2676
Size: 2342 3947
AleI CACnn'nnGTG
Cuts at: 1166 2849 3563 1166
Size: 1683 714 3892
Fragments arranged by size:
3892 1683 714
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2093 2125 2093
Size: 32 6257
AlwNI CAG_nnn'CTG
Cuts at: 2069 3499 4032 5604 2069
Size: 1430 533 1572 2754
Fragments arranged by size:
2754 1572 1430 533
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2120 2472 3060 3614 1870
Size: 73 134 43 352 588 554 4545
Fragments arranged by size:
4545 588 554 352 134 73 43
BamHI G'GATC_C
Cuts at: 3619 3986 3619
Size: 367 5922
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2338 3443 3538 3610
Size: 325 217 627 285 266 1105 95 72
Cuts at: 3610 5065 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1105 627 325 285 266 217
95 72
BanII G_rGCy'C
Cuts at: 1391 2121 2301 2662 2803 2870 2887 3101 3308
Size: 730 180 361 141 67 17 214 207
Cuts at: 3308 3382 3608 1391
Size: 74 226 4072
Fragments arranged by size:
4072 730 361 226 214 207 180 141
74 67 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2823 928
Size: 971 924 4394
Fragments arranged by size:
4394 971 924
BbvCI CC'TCA_GC
Cuts at: 2033 2033
Size: 6289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4480 4514 1903
Size: 34 2543 34 3678
Fragments arranged by size:
3678 2543 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3096 3209 4900
Size: 107 122 71 631 605 1424 113 1691
Cuts at: 4900 136
Size: 1525
Fragments arranged by size:
1691 1525 1424 631 605 122 113 107
71
BglII A'GATC_T
Cuts at: 2791 6284 2791
Size: 3493 2796
BlpI GC'TnA_GC
Cuts at: 2567 3706 2567
Size: 1139 5150
Bme1580I G_kGCm'C
Cuts at: 1790 3421 3446 3481 3543 3570 4350 5506 1790
Size: 1631 25 35 62 27 780 1156 2573
Fragments arranged by size:
2573 1631 1156 780 62 35 27 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6289
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2432 2665 3208
Size: 416 53 83 399 24 1015 233 543
Cuts at: 3208 3431 4020 4978 442
Size: 223 589 958 1753
Fragments arranged by size:
1753 1015 958 589 543 416 399 233
223 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 3200 2767
Size: 433 5856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 2664 4949 1239
Size: 1066 359 2285 2579
Fragments arranged by size:
2579 2285 1066 359
Bpu10I CC'TnA_GC
Cuts at: 2033 2033
Size: 6289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4414 5279 5577 4414
Size: 865 298 5126
Fragments arranged by size:
5126 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2918 3173 4952 882
Size: 617 1419 255 1779 2219
Fragments arranged by size:
2219 1779 1419 617 255
BsaAI yAC'GTr
Cuts at: 493 1204 3229 3490 4080 493
Size: 711 2025 261 590 2702
Fragments arranged by size:
2702 2025 711 590 261
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 4096 4478 5113
Size: 53 83 186 936 714 1849 382 635
Cuts at: 5113 275
Size: 1451
Fragments arranged by size:
1849 1451 936 714 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2590 2999 3528 4722 5120 5394 5541 5952 2590
Size: 409 529 1194 398 274 147 411 2927
Fragments arranged by size:
2927 1194 529 411 409 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6064
Fragments arranged by size:
6064 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2453 1186
Size: 943 324 5022
Fragments arranged by size:
5022 943 324
BseYI C'CCAG_C
Cuts at: 3289 3299 3543 5492 5988 3289
Size: 10 244 1949 496 3590
Fragments arranged by size:
3590 1949 496 244 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3087 3470 3488 3512 1163
Size: 573 1351 383 18 24 3940
Fragments arranged by size:
3940 1351 573 383 24 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2173 3469 3529 3739 4500 5528
Size: 275 186 172 1296 60 210 761 1028
Cuts at: 5528 1540
Size: 2301
Fragments arranged by size:
2301 1296 1028 761 275 210 186 172
60
BsiHKAI G_wGCw'C
Cuts at: 3585 4350 4435 5506 3585
Size: 765 85 1071 4368
Fragments arranged by size:
4368 1071 765 85
BsmI GAATG_Cn'
Cuts at: 2891 3800 3893 2891
Size: 909 93 5287
Fragments arranged by size:
5287 909 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2301 2662 2803 2870 2887 3101
Size: 399 331 180 361 141 67 17 214
Cuts at: 3101 3308 3382 3421 3446 3481 3543 3570 3585
Size: 207 74 39 25 35 62 27 15
Cuts at: 3585 3608 4350 4435 5506 1391
Size: 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 331 214 207
180 141 85 74 67 62 39 35
27 25 23 17 15
BspEI T'CCGG_A
Cuts at: 5952 5952
Size: 6289
BspHI T'CATG_A
Cuts at: 4178 5908 4178
Size: 1730 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 3294 3644 844
Size: 2155 295 350 3489
Fragments arranged by size:
3489 2155 350 295
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 4176 1394
Size: 429 1732 621 3507
Fragments arranged by size:
3507 1732 621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 2526 2613 4784 4958 65
Size: 1183 1149 129 87 2171 174 1396
Fragments arranged by size:
2171 1396 1183 1149 174 129 87
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 3039 3471 4933 5120 1700
Size: 110 346 883 432 1462 187 2869
Fragments arranged by size:
2869 1462 883 432 346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 2779 4349 5361 2779
Size: 1570 1012 3707
Fragments arranged by size:
3707 1570 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3499 794
Size: 2705 3584
BstBI TT'CG_AA
Cuts at: 5093 5093
Size: 6289
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6289
BstXI CCAn_nnnn'nTGG
Cuts at: 2329 2593 2329
Size: 264 6025
BstYI r'GATC_y
Cuts at: 1972 2791 3619 3986 4371 4388 5840 5926 5938
Size: 819 828 367 385 17 1452 86 12
Cuts at: 5938 6284 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 828 819 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2238 2739 3136 3570 513
Size: 552 939 90 144 501 397 434 3232
Fragments arranged by size:
3232 939 552 501 434 397 144 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2497 487
Size: 683 56 1271 4279
Fragments arranged by size:
4279 1271 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 2844 3805 4599 4619 2605
Size: 239 961 794 20 4275
Fragments arranged by size:
4275 961 794 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2509 2544 529
Size: 35 1945 35 4274
Fragments arranged by size:
4274 1945 35 35
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DraIII CAC_nnn'GTG
Cuts at: 2781 2781
Size: 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2425 3187 5296 809
Size: 507 1109 762 2109 1802
Fragments arranged by size:
2109 1802 1109 762 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2170 2544
Size: 54 1425 50 275 18 177 163 374
Cuts at: 2544 2691 3150 3210 3573 3736 4625 6254 8
Size: 147 459 60 363 163 889 1629 43
Fragments arranged by size:
1629 1425 889 459 374 363 275 177
163 163 147 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2170 3736 1537
Size: 275 358 1566 4090
Fragments arranged by size:
4090 1566 358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2962 3119 4219 1353
Size: 1609 157 1100 3423
Fragments arranged by size:
3423 1609 1100 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 3035 4876 5250 5396 387
Size: 1064 138 468 978 1841 374 146 1280
Fragments arranged by size:
1841 1280 1064 978 468 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 2664 4352 4949 5736 6004 1239
Size: 393 673 359 1688 597 787 268 1524
Fragments arranged by size:
1688 1524 787 673 597 393 359 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2036
Size: 6289
EcoO109I rG'GnC_Cy
Cuts at: 2125 2476 3496 2125
Size: 351 1020 4918
Fragments arranged by size:
4918 1020 351
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
FspI TGC'GCA
Cuts at: 4795 4795
Size: 6289
HaeII r_GCGC'y
Cuts at: 1678 2749 5436 1678
Size: 1071 2687 2531
Fragments arranged by size:
2687 2531 1071
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2662 2694 2939
Size: 32 135 32 704 32 354 32 245
Cuts at: 2939 2971 4930 4962 5004 5036 1373
Size: 32 1959 32 42 32 2626
Fragments arranged by size:
2626 1959 704 354 245 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3633 3879 669
Size: 988 281 1695 246 3079
Fragments arranged by size:
3079 1695 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 3078 5403 5587 1305
Size: 735 1038 2325 184 2007
Fragments arranged by size:
2325 2007 1038 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3152 3575 10
Size: 54 1425 343 1320 423 2724
Fragments arranged by size:
2724 1425 1320 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 2849 3563 4247 4606 4765 518
Size: 648 1425 258 714 684 359 159 2042
Fragments arranged by size:
2042 1425 714 684 648 359 258 159
MspA1I CmG'CkG
Cuts at: 1606 2554 3484 3547 3705 4382 5530 5775 1606
Size: 948 930 63 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 930 677 245 158 63
NaeI GCC'GGC
Cuts at: 3041 3473 3041
Size: 432 5857
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4798
Fragments arranged by size:
4798 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NgoMIV G'CCGG_C
Cuts at: 3039 3471 3039
Size: 432 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 3647 5192 1132
Size: 97 2418 1545 2229
Fragments arranged by size:
2418 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2836 1348
Size: 1488 4801
PciI A'CATG_T
Cuts at: 5188 5188
Size: 6289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2069 2781 3198 3289 1108
Size: 961 712 417 91 4108
Fragments arranged by size:
4108 961 712 417 91
PfoI T'CCnGG_A
Cuts at: 2127 2941 2127
Size: 814 5475
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PmlI CAC'GTG
Cuts at: 3490 3490
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 4358 4390 5871 5903 2762
Size: 32 1564 32 1481 32 3148
Fragments arranged by size:
3148 1564 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2125
Size: 6289
PshAI GACnn'nnGTC
Cuts at: 1613 3002 1613
Size: 1389 4900
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PstI C_TGCA'G
Cuts at: 830 2148 3387 3641 830
Size: 1318 1239 254 3478
Fragments arranged by size:
3478 1318 1239 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 3484 3484
Size: 6289
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SapI GCTCTTCn'nnn_
Cuts at: 2962 2962
Size: 6289
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SfcI C'TryA_G
Cuts at: 826 1045 2144 3383 3637 4772 5453 5644 826
Size: 219 1099 1239 254 1135 681 191 1471
Fragments arranged by size:
1471 1239 1135 1099 681 254 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3209 3209
Size: 6289
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 3471 1700
Size: 1771 4518
SmaI CCC'GGG
Cuts at: 1872 2079 2474 3062 3616 1872
Size: 207 395 588 554 4545
Fragments arranged by size:
4545 588 554 395 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4393 5294 5556 820
Size: 197 926 2450 901 262 1553
Fragments arranged by size:
2450 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4080 493
Size: 3587 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 3647 1132
Size: 2515 3774
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 3263 1517
Size: 1746 4543
StyI C'CwwG_G
Cuts at: 513 1065 2004 2729 2917 3728 513
Size: 552 939 725 188 811 3074
Fragments arranged by size:
3074 939 811 725 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2348 2999 3011 3281 4316 4333 4486
Size: 1255 768 651 12 270 1035 17 153
Cuts at: 4486 4671 5116 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1035 768 651 445 270 185
153 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4535 6247 96
Size: 274 80 33 51 1443 2558 1712 138
Fragments arranged by size:
2558 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6249 6281 6249
Size: 32 6257
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6289
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3091 3091
Size: 6289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 2077 2472 3060 3614 1870
Size: 207 395 588 554 4545
Fragments arranged by size:
4545 588 554 395 207
XmnI GAAnn'nnTTC
Cuts at: 4418 4418
Size: 6289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI