Restriction map for
pFN21AB0477
(Circular) (Six-base) MAPSORT of: pFN21AB0477.seq Check: 5080 from: 1 to: 6259
pFN21AB0477 6259 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2993 2993
Size: 6259
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3602 1937
Size: 1665 4594
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 2932 3110 4322 5706 5974 1632
Size: 896 404 178 1212 1384 268 1917
Fragments arranged by size:
1917 1384 1212 896 404 268 178
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 2670 5158 6135 1203
Size: 1467 2488 977 1327
Fragments arranged by size:
2488 1467 1327 977
AgeI A'CCGG_T
Cuts at: 2682 5090 2682
Size: 2408 3851
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 3053 1166
Size: 1887 4372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3180 3212 3180
Size: 32 6227
AlwNI CAG_nnn'CTG
Cuts at: 2290 2416 2506 2985 3080 3106 4002 5574 2290
Size: 126 90 479 95 26 896 1572 2975
Fragments arranged by size:
2975 1572 896 479 126 95 90 26
ApaI G_GGCC'C
Cuts at: 2362 2362
Size: 6259
ApaLI G'TGCA_C
Cuts at: 4316 5472 4316
Size: 1156 5103
ApoI r'AATT_y
Cuts at: 1493 2850 3568 3794 3946 6055 1493
Size: 1357 718 226 152 2109 1697
Fragments arranged by size:
2109 1697 1357 718 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2562 2610 3021 3584 1870
Size: 73 121 498 48 411 563 4545
Fragments arranged by size:
4545 563 498 411 121 73 48
BamHI G'GATC_C
Cuts at: 3589 3956 3589
Size: 367 5892
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 3580 5035 618
Size: 325 217 627 995 798 1455 1842
Fragments arranged by size:
1842 1455 995 798 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2232 2362 2713 3098 3578 1391
Size: 841 130 351 385 480 4072
Fragments arranged by size:
4072 841 480 385 351 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5288
BbvCI CC'TCA_GC
Cuts at: 2172 2291 2172
Size: 119 6140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4450 4484 1903
Size: 34 2513 34 3678
Fragments arranged by size:
3678 2513 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2366 4870 136
Size: 107 122 71 631 605 694 2504 1525
Fragments arranged by size:
2504 1525 694 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2881 3135 6254 2881
Size: 254 3119 2886
Fragments arranged by size:
3119 2886 254
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 2362 2425 2541 3438 4320 5476 1790
Size: 572 63 116 897 882 1156 2573
Fragments arranged by size:
2573 1156 897 882 572 116 63
BmgBI CAC'GTC
Cuts at: 1318 2536 1318
Size: 1218 5041
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3200 3990 4948
Size: 416 53 83 399 24 1783 790 958
Cuts at: 4948 442
Size: 1753
Fragments arranged by size:
1783 1753 958 790 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2577 3112 2266
Size: 311 535 5413
Fragments arranged by size:
5413 535 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2342 2727 2962 3107 3128 3419 3431
Size: 1007 96 385 235 145 21 291 12
Cuts at: 3431 3482 4919 1239
Size: 51 1437 2579
Fragments arranged by size:
2579 1437 1007 385 291 235 145 96
51 21 12
Bpu10I CC'TnA_GC
Cuts at: 2172 2291 2172
Size: 119 6140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 4384 5249 5547 2501
Size: 1883 865 298 3213
Fragments arranged by size:
3213 1883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4922 882
Size: 617 3423 2219
Fragments arranged by size:
3423 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2426 2671 3490 4050 493
Size: 711 1222 245 819 560 2702
Fragments arranged by size:
2702 1222 819 711 560 245
BsaBI GATnn'nnATC
Cuts at: 3134 6253 3134
Size: 3119 3140
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4066 4448 5083 275
Size: 53 83 186 936 2533 382 635 1451
Fragments arranged by size:
2533 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2124 2313 2439 2682 4692 5090 5364 5511 5922
Size: 189 126 243 2010 398 274 147 411
Cuts at: 5922 2124
Size: 2461
Fragments arranged by size:
2461 2010 411 398 274 243 189 147
126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3104 3134 3180 3210 3323
Size: 30 165 30 1701 30 46 30 113
Cuts at: 3323 3353 1178
Size: 30 4084
Fragments arranged by size:
4084 1701 165 113 46 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 3125 1186
Size: 1312 627 4320
Fragments arranged by size:
4320 1312 627
BseYI C'CCAG_C
Cuts at: 2475 3148 3326 5462 5958 2475
Size: 673 178 2136 496 2776
Fragments arranged by size:
2776 2136 673 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2411 3262 1163
Size: 573 675 851 4160
Fragments arranged by size:
4160 851 675 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3709 4470 5498 1540
Size: 275 186 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 2571 3098 3432 4320 4405 5476 2232
Size: 339 527 334 888 85 1071 3015
Fragments arranged by size:
3015 1071 888 527 339 334 85
BsmI GAATG_Cn'
Cuts at: 3770 3863 3770
Size: 93 6166
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2362 2425 2541 2571 2713 3098
Size: 399 442 130 63 116 30 142 385
Cuts at: 3098 3432 3438 3578 4320 4405 5476 1391
Size: 334 6 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 385 334 142
140 130 116 85 63 30 6
BspEI T'CCGG_A
Cuts at: 2313 2439 5922 2313
Size: 126 3483 2650
Fragments arranged by size:
3483 2650 126
BspHI T'CATG_A
Cuts at: 4148 5878 4148
Size: 1730 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 2505 2703 2964 2993 3614 844
Size: 1424 237 198 261 29 621 3489
Fragments arranged by size:
3489 1424 621 261 237 198 29
BsrBI CCG'CTC
Cuts at: 1394 1823 2188 4146 1394
Size: 429 365 1958 3507
Fragments arranged by size:
3507 1958 429 365
BsrDI GCAATG_nn'
Cuts at: 65 1248 4754 4928 65
Size: 1183 3506 174 1396
Fragments arranged by size:
3506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2682 4903 5090 1700
Size: 110 872 2221 187 2869
Fragments arranged by size:
2869 2221 872 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 2147 1925
Size: 222 6037
BssSI C'ACGA_G
Cuts at: 3431 4319 5331 3431
Size: 888 1012 4359
Fragments arranged by size:
4359 1012 888
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6259
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 3259 1184
Size: 2075 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 2381 2675 2381
Size: 294 5965
BstYI r'GATC_y
Cuts at: 1972 2310 2436 2881 3135 3589 3956 4341 4358
Size: 338 126 445 254 454 367 385 17
Cuts at: 4358 5810 5896 5908 6254 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 454 445 385 367 346 338
254 126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2797 2940 513
Size: 552 939 12 781 143 3832
Fragments arranged by size:
3832 939 781 552 143 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3373 487
Size: 683 56 2147 3373
Fragments arranged by size:
3373 2147 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 2811 3775 4569 4589 2358
Size: 453 964 794 20 4028
Fragments arranged by size:
4028 964 794 453 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6224
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DraIII CAC_nnn'GTG
Cuts at: 2941 3352 2941
Size: 411 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2540 2960 5266 809
Size: 507 1224 420 2306 1802
Fragments arranged by size:
2306 1802 1224 507 420
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2428 2677 3541
Size: 54 1425 50 275 18 598 249 864
Cuts at: 3541 3706 4595 6224 8
Size: 165 889 1629 43
Fragments arranged by size:
1629 1425 889 864 598 275 249 165
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3706 1537
Size: 275 1894 4090
Fragments arranged by size:
4090 1894 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 2322 4189 1353
Size: 849 120 1867 3423
Fragments arranged by size:
3423 1867 849 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 2747 4846 5220 5366 387
Size: 1064 138 681 477 2099 374 146 1280
Fragments arranged by size:
2099 1280 1064 681 477 374 146 138
EcoICRI GAG'CTC
Cuts at: 2230 3096 2230
Size: 866 5393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2342 2528 2727 2932 2962 3107
Size: 393 614 96 186 199 205 30 145
Cuts at: 3107 3110 3128 3419 3431 3482 4322 4919 5706
Size: 3 18 291 12 51 840 597 787
Cuts at: 5706 5974 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 614 597 393 291 268
205 199 186 145 96 51 30 18
12 3
EcoO109I rG'GnC_Cy
Cuts at: 2359 2398 2953 3077 3166 2359
Size: 39 555 124 89 5452
Fragments arranged by size:
5452 555 124 89 39
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
FspI TGC'GCA
Cuts at: 2197 4765 2197
Size: 2568 3691
HaeII r_GCGC'y
Cuts at: 1678 2029 2507 2662 5406 1678
Size: 351 478 155 2744 2531
Fragments arranged by size:
2744 2531 478 351 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 3104 3136 4900
Size: 32 135 32 489 32 1011 32 1764
Cuts at: 4900 4932 4974 5006 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1764 1011 489 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 3603 3849 669
Size: 988 281 796 869 246 3079
Fragments arranged by size:
3079 988 869 796 281 246
HindIII A'AGCT_T
Cuts at: 748 2750 748
Size: 2002 4257
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 2560 2629 3297 5373 5557 1305
Size: 801 454 69 668 2076 184 2007
Fragments arranged by size:
2076 2007 801 668 454 184 69
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2679 3543 10
Size: 54 1425 343 847 864 2726
Fragments arranged by size:
2726 1425 864 847 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2774 2780 3053 4217 4576 4735 518
Size: 648 1608 6 273 1164 359 159 2042
Fragments arranged by size:
2042 1608 1164 648 359 273 159 6
MspA1I CmG'CkG
Cuts at: 1606 2176 2299 2339 2799 2903 3103 3675 4352
Size: 570 123 40 460 104 200 572 677
Cuts at: 4352 5500 5745 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 572 570 460 245 200
123 104 40
NcoI C'CATG_G
Cuts at: 513 1065 2004 2016 513
Size: 552 939 12 4756
Fragments arranged by size:
4756 939 552 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 2202 3617 5162 1132
Size: 97 973 1415 1545 2229
Fragments arranged by size:
2229 1545 1415 973 97
PasI CC'CwG_GG
Cuts at: 1348 2466 3080 1348
Size: 1118 614 4527
Fragments arranged by size:
4527 1118 614
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2941 3190 1108
Size: 1833 249 4177
Fragments arranged by size:
4177 1833 249
PfoI T'CCnGG_A
Cuts at: 3168 3396 3168
Size: 228 6031
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PmlI CAC'GTG
Cuts at: 2426 2671 3490 2426
Size: 245 819 5195
Fragments arranged by size:
5195 819 245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2506 3180 3212 4328 4360 5841 5873 2474
Size: 32 674 32 1116 32 1481 32 2860
Fragments arranged by size:
2860 1481 1116 674 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2398 2953 3166 2398
Size: 555 213 5491
Fragments arranged by size:
5491 555 213
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6259
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PspOMI G'GGCC_C
Cuts at: 2358 2358
Size: 6259
PspXI vC'TCGA_Gb
Cuts at: 2562 2562
Size: 6259
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2120 2152 2120
Size: 32 6227
PstI C_TGCA'G
Cuts at: 830 2304 2502 2721 3339 3611 830
Size: 1474 198 219 618 272 3478
Fragments arranged by size:
3478 1474 618 272 219 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 2176 2299 2339 3103 2176
Size: 123 40 764 5332
Fragments arranged by size:
5332 764 123 40
SacI G_AGCT'C
Cuts at: 2232 3098 2232
Size: 866 5393
SacII CC_GC'GG
Cuts at: 2800 2800
Size: 6259
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SbfI CC_TGCA'GG
Cuts at: 2502 3611 2502
Size: 1109 5150
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 2109 2158 2300 2498 2717 3335 3607
Size: 219 1064 49 142 198 219 618 272
Cuts at: 3607 4742 5423 5614 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 681 618 272 219 219
198 191 142 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 2066 3023 3586 1872
Size: 194 957 563 4545
Fragments arranged by size:
4545 957 563 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 2562 4363 5264 5526 820
Size: 197 926 537 82 1801 901 262 1553
Fragments arranged by size:
1801 1553 926 901 537 262 197 82
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 2202 3617 1132
Size: 1070 1415 3774
Fragments arranged by size:
3774 1415 1070
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 3227 1517
Size: 1710 4549
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2093 3051 3698 513
Size: 552 939 12 77 958 647 3074
Fragments arranged by size:
3074 958 939 647 552 77 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2544 3197 3335 4286 4303 4456 4641
Size: 1255 964 653 138 951 17 153 185
Cuts at: 4641 5086 325
Size: 445 1498
Fragments arranged by size:
1498 1255 964 951 653 445 185 153
138 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3037 3501 4505
Size: 274 80 33 51 1443 1060 464 1004
Cuts at: 4505 6217 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1060 1004 464 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6259
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2624 3187 3323 2624
Size: 563 136 5560
Fragments arranged by size:
5560 563 136
XhoI C'TCGA_G
Cuts at: 1943 2562 1943
Size: 619 5640
XmaI C'CCGG_G
Cuts at: 1870 2064 3021 3584 1870
Size: 194 957 563 4545
Fragments arranged by size:
4545 957 563 194
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI RsrII SanDI
SapI SexAI SfiI SfoI SrfI SwaI