Restriction map for
pFN21AB0476
(Circular) (Six-base) MAPSORT of: pFN21AB0476.seq Check: 2711 from: 1 to: 7762
pFN21AB0476 7762 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5572 278
Size: 53 83 186 936 4036 2468
Fragments arranged by size:
4036 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4681 5105 1937
Size: 2744 424 4594
Fragments arranged by size:
4594 2744 424
Acc65I G'GTAC_C
Cuts at: 2010 5083 2010
Size: 3073 4689
AclI AA'CG_TT
Cuts at: 1563 5889 6262 1563
Size: 4326 373 3063
Fragments arranged by size:
4326 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 2632 2853 2954 4098 4489 4992 5825
Size: 567 433 221 101 1144 391 503 833
Cuts at: 5825 7209 7477 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1144 833 567 503 433 391
268 221 101
AflII C'TTAA_G
Cuts at: 820 1017 2320 3053 4139 820
Size: 197 1303 733 1086 4443
Fragments arranged by size:
4443 1303 1086 733 197
AflIII A'CryG_T
Cuts at: 1203 3160 3183 6661 7638 1203
Size: 1957 23 3478 977 1327
Fragments arranged by size:
3478 1957 1327 977 23
AgeI A'CCGG_T
Cuts at: 6593 6593
Size: 7762
AhdI GACnn_n'nnGTC
Cuts at: 6491 6491
Size: 7762
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7762
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2865 2897 2865
Size: 32 7730
AlwNI CAG_nnn'CTG
Cuts at: 4162 5505 7077 4162
Size: 1343 1572 4847
Fragments arranged by size:
4847 1572 1343
ApaLI G'TGCA_C
Cuts at: 2279 5819 6975 2279
Size: 3540 1156 3066
Fragments arranged by size:
3540 3066 1156
ApoI r'AATT_y
Cuts at: 1493 2654 2732 2864 3377 3566 3590 3604 3734
Size: 1161 78 132 513 189 24 14 130
Cuts at: 3734 4817 4939 4994 5071 5297 5449 7558 1493
Size: 1083 122 55 77 226 152 2109 1697
Fragments arranged by size:
2109 1697 1161 1083 513 226 189 152
132 130 122 78 77 55 24 14
AseI AT'TA_AT
Cuts at: 160 1032 3129 6316 160
Size: 872 2097 3187 1606
Fragments arranged by size:
3187 2097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
AvaI C'yCGr_G
Cuts at: 1870 1943 3105 5087 1870
Size: 73 1162 1982 4545
Fragments arranged by size:
4545 1982 1162 73
BamHI G'GATC_C
Cuts at: 2299 5092 5459 2299
Size: 2793 367 4602
Fragments arranged by size:
4602 2793 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 2069 2483 5083 6538
Size: 325 217 627 223 59 414 2600 1455
Cuts at: 6538 618
Size: 1842
Fragments arranged by size:
2600 1842 1455 627 414 325 223 217
59
BanII G_rGCy'C
Cuts at: 1391 3106 3801 4434 5081 1391
Size: 1715 695 633 647 4072
Fragments arranged by size:
4072 1715 695 647 633
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 7762
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2187 4248 4495 928
Size: 971 288 2061 247 4195
Fragments arranged by size:
4195 2061 971 288 247
BbvCI CC'TCA_GC
Cuts at: 4155 4155
Size: 7762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5953 5987 1903
Size: 34 50 34 3932 34 3678
Fragments arranged by size:
3932 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3655 4876 5654 6864 3655
Size: 1221 778 1210 4553
Fragments arranged by size:
4553 1221 1210 778
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7762
BfrBI ATG'CAT
Cuts at: 3827 3827
Size: 7762
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6373 136
Size: 107 122 71 631 605 4701 1525
Fragments arranged by size:
4701 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7757 7757
Size: 7762
BlpI GC'TnA_GC
Cuts at: 5179 5179
Size: 7762
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2488 2519 5823 6979 1790
Size: 493 205 31 3304 1156 2573
Fragments arranged by size:
3304 2573 1156 493 205 31
BmgBI CAC'GTC
Cuts at: 1318 4517 1318
Size: 3199 4563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3493 4735 5493
Size: 416 53 83 399 24 2076 1242 758
Cuts at: 5493 6451 442
Size: 958 1753
Fragments arranged by size:
2076 1753 1242 958 758 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2021 3845 1056
Size: 965 1824 4973
Fragments arranged by size:
4973 1824 965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2620 2702 4448 6422 1239
Size: 1381 82 1746 1974 2579
Fragments arranged by size:
2579 1974 1746 1381 82
Bpu10I CC'TnA_GC
Cuts at: 4155 4155
Size: 7762
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3207 3815 3871 4561 5887 6752 7050 3207
Size: 608 56 690 1326 865 298 3919
Fragments arranged by size:
3919 1326 865 690 608 298 56
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6425 882
Size: 617 4926 2219
Fragments arranged by size:
4926 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3209 5553 493
Size: 711 2005 2344 2702
Fragments arranged by size:
2702 2344 2005 711
BsaBI GATnn'nnATC
Cuts at: 7756 7756
Size: 7762
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 5569 5951 6586
Size: 53 83 186 936 537 3499 382 635
Cuts at: 6586 275
Size: 1451
Fragments arranged by size:
3499 1451 936 635 537 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2086 3520 6195 6593 6867 7014 7425 2086
Size: 1434 2675 398 274 147 411 2423
Fragments arranged by size:
2675 2423 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2158 2188 4143
Size: 30 165 30 678 30 47 30 1955
Cuts at: 4143 4173 1178
Size: 30 4767
Fragments arranged by size:
4767 1955 678 165 47 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3791 1186
Size: 2605 5157
BseYI C'CCAG_C
Cuts at: 2045 3066 4743 6965 7461 2045
Size: 1021 1677 2222 496 2346
Fragments arranged by size:
2346 2222 1677 1021 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2015 3231 4157 1163
Size: 573 279 1216 926 4768
Fragments arranged by size:
4768 1216 926 573 279
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5212 5973 7001 1540
Size: 275 186 3211 761 1028 2301
Fragments arranged by size:
3211 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 3354 3801 4434 5823 5908 6979 2283
Size: 1071 447 633 1389 85 1071 3066
Fragments arranged by size:
3066 1389 1071 1071 633 447 85
BsmI GAATG_Cn'
Cuts at: 2560 3262 5273 5366 2560
Size: 702 2011 93 4956
Fragments arranged by size:
4956 2011 702 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2488 2519 3106 3354 3801 4434
Size: 399 493 205 31 587 248 447 633
Cuts at: 4434 5081 5823 5908 6979 1391
Size: 647 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 647 633 587 493 447
399 248 205 85 31
BspEI T'CCGG_A
Cuts at: 2086 7425 2086
Size: 5339 2423
BspHI T'CATG_A
Cuts at: 2467 5651 7381 2467
Size: 3184 1730 2848
Fragments arranged by size:
3184 2848 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5117 844
Size: 4273 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 5649 1394
Size: 429 235 3591 3507
Fragments arranged by size:
3591 3507 429 235
BsrDI GCAATG_nn'
Cuts at: 65 1248 2855 6257 6431 65
Size: 1183 1607 3402 174 1396
Fragments arranged by size:
3402 1607 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 6406 6593 1700
Size: 110 203 4393 187 2869
Fragments arranged by size:
4393 2869 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7762
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7762
BssSI C'ACGA_G
Cuts at: 5822 6834 5822
Size: 1012 6750
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4844 794
Size: 4050 3712
BstBI TT'CG_AA
Cuts at: 6566 6566
Size: 7762
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7762
BstXI CCAn_nnnn'nTGG
Cuts at: 2764 4891 2764
Size: 2127 5635
BstYI r'GATC_y
Cuts at: 1972 2299 5092 5459 5844 5861 7313 7399 7411
Size: 327 2793 367 385 17 1452 86 12
Cuts at: 7411 7757 1972
Size: 346 1977
Fragments arranged by size:
2793 1977 1452 385 367 346 327 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2250 3293 4192 4611 513
Size: 552 1185 1043 899 419 3664
Fragments arranged by size:
3664 1185 1043 899 552 419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7023
Fragments arranged by size:
7023 683 56
BtsI GCAGTG_nn'
Cuts at: 2039 5278 6072 6092 2039
Size: 3239 794 20 3709
Fragments arranged by size:
3709 3239 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4351 4386 529
Size: 35 3787 35 3905
Fragments arranged by size:
3905 3787 35 35
DraI TTT'AAA
Cuts at: 3277 3571 4788 5066 3277
Size: 294 1217 278 5973
Fragments arranged by size:
5973 1217 294 278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6769 809
Size: 507 5453 1802
Fragments arranged by size:
5453 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 5209 6098
Size: 54 1425 50 275 18 381 2998 889
Cuts at: 6098 7727 8
Size: 1629 43
Fragments arranged by size:
2998 1629 1425 889 381 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5209 1537
Size: 275 3397 4090
Fragments arranged by size:
4090 3397 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3339 3477 4439 4635 5692 1353
Size: 1986 138 962 196 1057 3423
Fragments arranged by size:
3423 1986 1057 962 196 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6349 6723 6869 387
Size: 1064 138 4760 374 146 1280
Fragments arranged by size:
4760 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3799 4432 3799
Size: 633 7129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2620 2632 2702 2853 2954 4098
Size: 393 567 421 12 70 151 101 1144
Cuts at: 4098 4448 4489 4992 5825 6422 7209 7477 1239
Size: 350 41 503 833 597 787 268 1524
Fragments arranged by size:
1524 1144 833 787 597 567 503 421
393 350 268 151 101 70 41 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3344 4323 3344
Size: 979 6783
EcoO109I rG'GnC_Cy
Cuts at: 3029 3218 3029
Size: 189 7573
EcoRI G'AATT_C
Cuts at: 4994 5071 5449 4994
Size: 77 378 7307
Fragments arranged by size:
7307 378 77
EcoRV GAT'ATC
Cuts at: 4810 4810
Size: 7762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2249 2912 2249
Size: 663 7099
FspI TGC'GCA
Cuts at: 4475 6268 4475
Size: 1793 5969
HaeII r_GCGC'y
Cuts at: 1678 2073 2095 6909 1678
Size: 395 22 4814 2531
Fragments arranged by size:
4814 2531 395 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 3844 3876 4536
Size: 32 135 32 674 32 1566 32 660
Cuts at: 4536 4568 4722 4754 6403 6435 6477 6509 1373
Size: 32 154 32 1649 32 42 32 2626
Fragments arranged by size:
2626 1649 1566 674 660 154 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4682 5106 5352 669
Size: 988 281 2744 424 246 3079
Fragments arranged by size:
3079 2744 988 424 281 246
HindIII A'AGCT_T
Cuts at: 748 4596 748
Size: 3848 3914
HpaI GTT'AAC
Cuts at: 5352 5352
Size: 7762
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 7762
KpnI G_GTAC'C
Cuts at: 2014 5087 2014
Size: 3073 4689
MfeI C'AATT_G
Cuts at: 2612 5361 2612
Size: 2749 5013
MluI A'CGCG_T
Cuts at: 7638 7638
Size: 7762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 2781 4891 6876 7060 1305
Size: 972 504 2110 1985 184 2007
Fragments arranged by size:
2110 2007 1985 972 504 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 10
Size: 54 1425 343 381 5559
Fragments arranged by size:
5559 1425 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 3131 4044 5720 6079 6238 518
Size: 648 1165 800 913 1676 359 159 2042
Fragments arranged by size:
2042 1676 1165 913 800 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3359 4291 4509 5178 5855 7003 7248 1606
Size: 1753 932 218 669 677 1148 245 2120
Fragments arranged by size:
2120 1753 1148 932 677 669 245 218
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 7762
NcoI C'CATG_G
Cuts at: 513 1065 4192 4611 513
Size: 552 3127 419 3664
Fragments arranged by size:
3664 3127 552 419
NdeI CA'TA_TG
Cuts at: 387 2340 3829 387
Size: 1953 1489 4320
Fragments arranged by size:
4320 1953 1489
NheI G'CTAG_C
Cuts at: 1052 2017 3841 1052
Size: 965 1824 4973
Fragments arranged by size:
4973 1824 965
NotI GC'GGCC_GC
Cuts at: 5209 5209
Size: 7762
NsiI A_TGCA'T
Cuts at: 3829 3829
Size: 7762
NspI r_CATG'y
Cuts at: 1132 1229 2408 3164 3187 4049 4262 4402 5120
Size: 97 1179 756 23 862 213 140 718
Cuts at: 5120 6665 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1179 862 756 718 213 140
97 23
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7762
PciI A'CATG_T
Cuts at: 3160 3183 6661 3160
Size: 23 3478 4261
Fragments arranged by size:
4261 3478 23
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7762
PfoI T'CCnGG_A
Cuts at: 3031 3031
Size: 7762
PmeI GTTT'AAAC
Cuts at: 5066 5066
Size: 7762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5831 5863 7344 7376 5831
Size: 32 1481 32 6217
Fragments arranged by size:
6217 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3029 3218 3029
Size: 189 7573
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7762
PsiI TTA'TAA
Cuts at: 5332 5332
Size: 7762
PstI C_TGCA'G
Cuts at: 830 5114 830
Size: 4284 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7762
PvuII CAG'CTG
Cuts at: 4291 4291
Size: 7762
SacI G_AGCT'C
Cuts at: 3801 4434 3801
Size: 633 7129
SalI G'TCGA_C
Cuts at: 1936 4680 5104 1936
Size: 2744 424 4594
Fragments arranged by size:
4594 2744 424
SapI GCTCTTCn'nnn_
Cuts at: 4439 4439
Size: 7762
SbfI CC_TGCA'GG
Cuts at: 5114 5114
Size: 7762
ScaI AGT'ACT
Cuts at: 2229 6010 7722 2229
Size: 3781 1712 2269
Fragments arranged by size:
3781 2269 1712
SfcI C'TryA_G
Cuts at: 826 1045 2625 5110 6245 6926 7117 826
Size: 219 1580 2485 1135 681 191 1471
Fragments arranged by size:
2485 1580 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 7762
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7762
SmaI CCC'GGG
Cuts at: 1872 5089 1872
Size: 3217 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2320 3053 3222 3794 3850 4139
Size: 197 926 377 733 169 572 56 289
Cuts at: 4139 4540 5866 6767 7029 820
Size: 401 1326 901 262 1553
Fragments arranged by size:
1553 1326 926 901 733 572 401 377
289 262 197 169 56
SnaBI TAC'GTA
Cuts at: 493 5553 493
Size: 5060 2702
SpeI A'CTAG_T
Cuts at: 152 2429 152
Size: 2277 5485
SphI G_CATG'C
Cuts at: 1132 2408 4049 5120 1132
Size: 1276 1641 1071 3774
Fragments arranged by size:
3774 1641 1276 1071
SspI AAT'ATT
Cuts at: 5 52 2311 3324 3365 5686 5
Size: 47 2259 1013 41 2321 2081
Fragments arranged by size:
2321 2259 2081 1013 47 41
StuI AGG'CCT
Cuts at: 1517 4145 4646 1517
Size: 2628 501 4633
Fragments arranged by size:
4633 2628 501
StyI C'CwwG_G
Cuts at: 513 1065 4192 4479 4611 4800 4850 5201 513
Size: 552 3127 287 132 189 50 351 3074
Fragments arranged by size:
3127 3074 552 351 287 189 132 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 5006 5789 5806 5959 6144 6589
Size: 1255 1578 1848 783 17 153 185 445
Cuts at: 6589 325
Size: 1498
Fragments arranged by size:
1848 1578 1498 1255 783 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2227 3501 4053
Size: 274 80 33 51 1443 250 1274 552
Cuts at: 4053 6008 7720 96
Size: 1955 1712 138
Fragments arranged by size:
1955 1712 1443 1274 552 274 250 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2081 2113 7722 7754 2081
Size: 32 5609 32 2089
Fragments arranged by size:
5609 2089 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7762
XbaI T'CTAG_A
Cuts at: 5098 5098
Size: 7762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4486 4486
Size: 7762
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7762
XmaI C'CCGG_G
Cuts at: 1870 5087 1870
Size: 3217 4545
XmnI GAAnn'nnTTC
Cuts at: 3594 4185 4977 5891 3594
Size: 591 792 914 5465
Fragments arranged by size:
5465 914 792 591
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5570 276
Size: 53 83 186 936 4036 2468
Fragments arranged by size:
4036 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI