Restriction map for
pFN21AB0475
(Circular) (Six-base) MAPSORT of: pFN21AB0475.seq Check: 8412 from: 1 to: 6256
pFN21AB0475 6256 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2525 2816 2525
Size: 291 5965
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2367 3599 1937
Size: 430 1232 4594
Fragments arranged by size:
4594 1232 430
Acc65I G'GTAC_C
Cuts at: 2459 2778 3577 2459
Size: 319 799 5138
Fragments arranged by size:
5138 799 319
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4319 5703 5971 1632
Size: 2687 1384 268 1917
Fragments arranged by size:
2687 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 3186 5155 6132 1203
Size: 1983 1969 977 1327
Fragments arranged by size:
1983 1969 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 2141 1166
Size: 975 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 2837 3250 3999 5571 2837
Size: 413 749 1572 3522
Fragments arranged by size:
3522 1572 749 413
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 2297 3008 3565 3791 3943 6052 1493
Size: 804 711 557 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 711 557 226 152
AscI GG'CGCG_CC
Cuts at: 3046 3046
Size: 6256
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 3581 1870
Size: 73 1638 4545
Fragments arranged by size:
4545 1638 73
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2459 2644 2778 3058
Size: 325 217 627 240 432 185 134 280
Cuts at: 3058 3144 3577 5032 618
Size: 86 433 1455 1842
Fragments arranged by size:
1842 1455 627 433 432 325 280 240
217 185 134 86
BanII G_rGCy'C
Cuts at: 1391 3575 1391
Size: 2184 4072
BbeI G_GCGC'C
Cuts at: 2031 3148 2031
Size: 1117 5139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5285
BbvCI CC'TCA_GC
Cuts at: 2524 2524
Size: 6256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2563 4148 5358 2563
Size: 1585 1210 3461
Fragments arranged by size:
3461 1585 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 2649 3063 4317 5473 1790
Size: 859 414 1254 1156 2573
Fragments arranged by size:
2573 1254 1156 859 414
BmgBI CAC'GTC
Cuts at: 1318 2143 2797 1318
Size: 825 654 4777
Fragments arranged by size:
4777 825 654
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2538 3305 3987
Size: 416 53 83 399 24 1121 767 682
Cuts at: 3987 4945 442
Size: 958 1753
Fragments arranged by size:
1753 1121 958 767 682 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2372 4916 1239
Size: 1127 6 2544 2579
Fragments arranged by size:
2579 2544 1127 6
Bpu10I CC'TnA_GC
Cuts at: 2524 2980 3480 2524
Size: 456 500 5300
Fragments arranged by size:
5300 500 456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4381 5246 5544 4381
Size: 865 298 5093
Fragments arranged by size:
5093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3187 3553 4047 493
Size: 711 1983 366 494 2702
Fragments arranged by size:
2702 1983 711 494 366
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2085 3145 3393
Size: 53 83 186 936 495 57 1060 248
Cuts at: 3393 4063 4445 5080 275
Size: 670 382 635 1451
Fragments arranged by size:
1451 1060 936 670 635 495 382 248
186 83 57 53
BsaWI w'CCGG_w
Cuts at: 2598 3453 4689 5087 5361 5508 5919 2598
Size: 855 1236 398 274 147 411 2935
Fragments arranged by size:
2935 1236 855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3010 3040 1178
Size: 30 165 30 1607 30 4394
Fragments arranged by size:
4394 1607 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3018 3329 3332 3335 3338 3341 3344 3347
Size: 1832 311 3 3 3 3 3 3
Cuts at: 3347 1186
Size: 4095
Fragments arranged by size:
4095 1832 311 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2177 2648 2830 3222 5459 5955 2177
Size: 471 182 392 2237 496 2478
Fragments arranged by size:
2478 2237 496 471 392 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2503 2753 1163
Size: 573 399 368 250 4666
Fragments arranged by size:
4666 573 399 368 250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4317 4402 5473 4317
Size: 85 1071 5100
Fragments arranged by size:
5100 1071 85
BsmI GAATG_Cn'
Cuts at: 2069 3767 3860 2069
Size: 1698 93 4465
Fragments arranged by size:
4465 1698 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3030 3030
Size: 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2649 3063 3575 4317 4402 5473 1391
Size: 399 859 414 512 742 85 1071 2174
Fragments arranged by size:
2174 1071 859 742 512 414 399 85
BspEI T'CCGG_A
Cuts at: 2598 5919 2598
Size: 3321 2935
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2525 2790 2816 3258 3611 844
Size: 1681 265 26 442 353 3489
Fragments arranged by size:
3489 1681 442 353 265 26
BsrBI CCG'CTC
Cuts at: 1394 1823 4143 1394
Size: 429 2320 3507
Fragments arranged by size:
3507 2320 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3055 3147 3287 4900 5087 1700
Size: 110 1245 92 140 1613 187 2869
Fragments arranged by size:
2869 1613 1245 187 140 110 92
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 3046 1925
Size: 1121 5135
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2749 1184
Size: 1565 4691
BstXI CCAn_nnnn'nTGG
Cuts at: 2881 2881
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 2595 3586 3953 4338 4355 5807 5893 5905
Size: 623 991 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 991 623 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2745 2745
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 3063 3414 513
Size: 552 939 252 807 351 3355
Fragments arranged by size:
3355 939 807 552 351 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 2152 2475 3772 4566 4586 2152
Size: 323 1297 794 20 3822
Fragments arranged by size:
3822 1297 794 323 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DraIII CAC_nnn'GTG
Cuts at: 3299 3299
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2433 2511
Size: 54 1425 50 275 18 408 195 78
Cuts at: 2511 3066 3703 4592 6221 8
Size: 555 637 889 1629 43
Fragments arranged by size:
1629 1425 889 637 555 408 275 195
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2909 4186 1353
Size: 1556 1277 3423
Fragments arranged by size:
3423 1556 1277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2321 4843 5217 5363 387
Size: 1064 138 732 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 732 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2372 4319 4916 5703 5971 1239
Size: 393 734 6 1947 597 787 268 1524
Fragments arranged by size:
1947 1524 787 734 597 393 268 6
EcoO109I rG'GnC_Cy
Cuts at: 2503 2915 2940 2503
Size: 412 25 5819
Fragments arranged by size:
5819 412 25
EcoRI G'AATT_C
Cuts at: 2297 3008 3565 3943 2297
Size: 711 557 378 4610
Fragments arranged by size:
4610 711 557 378
FseI GG_CCGG'CC
Cuts at: 3291 3291
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2031 3148 5403 1678
Size: 353 1117 2255 2531
Fragments arranged by size:
2531 2255 1117 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2040 2072 4897 4929 4971
Size: 32 135 32 468 32 2825 32 42
Cuts at: 4971 5003 1373
Size: 32 2626
Fragments arranged by size:
2825 2626 468 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2689 3600 3846 669
Size: 988 281 751 911 246 3079
Fragments arranged by size:
3079 988 911 751 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2027 3144 2027
Size: 1117 5139
KpnI G_GTAC'C
Cuts at: 2463 2782 3581 2463
Size: 319 799 5138
Fragments arranged by size:
5138 799 319
MfeI C'AATT_G
Cuts at: 2446 3855 2446
Size: 1409 4847
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 2435 2513 10
Size: 54 1425 343 408 195 78 3753
Fragments arranged by size:
3753 1425 408 343 195 78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2261 2429 3189 4214 4573 4732
Size: 648 975 120 168 760 1025 359 159
Cuts at: 4732 518
Size: 2042
Fragments arranged by size:
2042 1025 975 760 648 359 168 159
120
MspA1I CmG'CkG
Cuts at: 1606 2033 2788 3487 3672 4349 5497 5742 1606
Size: 427 755 699 185 677 1148 245 2120
Fragments arranged by size:
2120 1148 755 699 677 427 245 185
NaeI GCC'GGC
Cuts at: 3149 3289 3149
Size: 140 6116
NarI GG'CG_CC
Cuts at: 2028 3145 2028
Size: 1117 5139
NcoI C'CATG_G
Cuts at: 513 1065 2004 2256 513
Size: 552 939 252 4513
Fragments arranged by size:
4513 939 552 252
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 3147 3287 3147
Size: 140 6116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 3614 5159 1132
Size: 97 2385 1545 2229
Fragments arranged by size:
2385 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2448 3299 1108
Size: 1340 851 4065
Fragments arranged by size:
4065 1340 851
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PmlI CAC'GTG
Cuts at: 3187 3187
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2915 2940 2503
Size: 412 25 5819
Fragments arranged by size:
5819 412 25
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 6224
PstI C_TGCA'G
Cuts at: 830 2159 2787 3255 3608 830
Size: 1329 628 468 353 3478
Fragments arranged by size:
3478 1329 628 468 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 3487 3487
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SanDI GG'GwC_CC
Cuts at: 2915 2915
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SfcI C'TryA_G
Cuts at: 826 1045 2155 2783 2926 3169 3202 3251 3604
Size: 219 1110 628 143 243 33 49 353
Cuts at: 3604 4739 5420 5611 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1110 681 628 353 243 219
191 143 49 33
SfoI GGC'GCC
Cuts at: 2029 3146 2029
Size: 1117 5139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4360 5261 5523 820
Size: 197 926 2417 901 262 1553
Fragments arranged by size:
2417 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 2673 2874 3695 513
Size: 552 939 252 417 201 821 3074
Fragments arranged by size:
3074 939 821 552 417 252 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 3246 3287 4283 4300 4453 4638
Size: 1255 627 1039 41 996 17 153 185
Cuts at: 4638 5083 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1039 996 627 445 185 153
41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4502 6214 96
Size: 274 80 33 51 1443 2525 1712 138
Fragments arranged by size:
2525 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2654 2686 3010 3042 6216 6248 2654
Size: 32 324 32 3174 32 2662
Fragments arranged by size:
3174 2662 324 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 1316
Size: 1185 5071
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2233 2011
Size: 222 6034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 4385 4385
Size: 6256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI
PacI PfoI PspOMI PspXI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI