Restriction map for
pFN21AB0473
(Circular) (Six-base) MAPSORT of: pFN21AB0473.seq Check: 733 from: 1 to: 6202
pFN21AB0473 6202 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2791 2791
Size: 6202
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2217 3206 3545 1937
Size: 280 989 339 4594
Fragments arranged by size:
4594 989 339 280
Acc65I G'GTAC_C
Cuts at: 2861 3523 2861
Size: 662 5540
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2060 3477 4265 5649 5917 1632
Size: 428 1417 788 1384 268 1917
Fragments arranged by size:
1917 1417 1384 788 428 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 2301 5101 6078 1203
Size: 1098 2800 977 1327
Fragments arranged by size:
2800 1327 1098 977
AgeI A'CCGG_T
Cuts at: 5033 5033
Size: 6202
AhdI GACnn_n'nnGTC
Cuts at: 4931 4931
Size: 6202
AleI CACnn'nnGTG
Cuts at: 1166 2969 1166
Size: 1803 4399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6202
AlwNI CAG_nnn'CTG
Cuts at: 2804 2813 2914 3187 3945 5517 2804
Size: 9 101 273 758 1572 3489
Fragments arranged by size:
3489 1572 758 273 101 9
ApaLI G'TGCA_C
Cuts at: 2522 4259 5415 2522
Size: 1737 1156 3309
Fragments arranged by size:
3309 1737 1156
ApoI r'AATT_y
Cuts at: 1493 3511 3737 3889 5998 1493
Size: 2018 226 152 2109 1697
Fragments arranged by size:
2109 2018 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2598 3101 3151 3527 1870
Size: 73 114 541 503 50 376 4545
Fragments arranged by size:
4545 541 503 376 114 73 50
BamHI G'GATC_C
Cuts at: 3532 3899 3532
Size: 367 5835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2861 2950 3523 4978 618
Size: 325 217 627 1074 89 573 1455 1842
Fragments arranged by size:
1842 1455 1074 627 573 325 217 89
BanII G_rGCy'C
Cuts at: 1391 2228 3069 3521 1391
Size: 837 841 452 4072
Fragments arranged by size:
4072 841 837 452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2670 928
Size: 971 771 4460
Fragments arranged by size:
4460 971 771
BbvCI CC'TCA_GC
Cuts at: 2481 2481
Size: 6202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4393 4427 1903
Size: 34 2456 34 3678
Fragments arranged by size:
3678 2456 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4094 5304 4094
Size: 1210 4992
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3413 4813 136
Size: 107 122 71 631 605 1741 1400 1525
Fragments arranged by size:
1741 1525 1400 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3366 6197 3366
Size: 2831 3371
BlpI GC'TnA_GC
Cuts at: 2181 3125 3619 2181
Size: 944 494 4764
Fragments arranged by size:
4764 944 494
Bme1580I G_kGCm'C
Cuts at: 1790 2526 2873 2921 4263 5419 1790
Size: 736 347 48 1342 1156 2573
Fragments arranged by size:
2573 1342 1156 736 347 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2650 3933 4891
Size: 416 53 83 399 24 1233 1283 958
Cuts at: 4891 442
Size: 1753
Fragments arranged by size:
1753 1283 1233 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2790 2976 3083 4862 1239
Size: 1551 186 107 1779 2579
Fragments arranged by size:
2579 1779 1551 186 107
Bpu10I CC'TnA_GC
Cuts at: 2481 2829 2481
Size: 348 5854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4327 5192 5490 4327
Size: 865 298 5039
Fragments arranged by size:
5039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3062 4865 882
Size: 617 1563 1803 2219
Fragments arranged by size:
2219 1803 1563 617
BsaAI yAC'GTr
Cuts at: 493 1204 2221 2512 3993 493
Size: 711 1017 291 1481 2702
Fragments arranged by size:
2702 1481 1017 711 291
BsaBI GATnn'nnATC
Cuts at: 6196 6196
Size: 6202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 4009 4391 5026
Size: 53 83 186 936 521 1955 382 635
Cuts at: 5026 275
Size: 1451
Fragments arranged by size:
1955 1451 936 635 521 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4635 5033 5307 5454 5865 4635
Size: 398 274 147 411 4972
Fragments arranged by size:
4972 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2838 2868 1178
Size: 30 165 30 1435 30 4512
Fragments arranged by size:
4512 1435 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 3104 3293 3353 3356 1186
Size: 1312 606 189 60 3 4032
Fragments arranged by size:
4032 1312 606 189 60 3
BseYI C'CCAG_C
Cuts at: 2035 2242 2407 2438 2457 2857 2890 2907 2977
Size: 207 165 31 19 400 33 17 70
Cuts at: 2977 3052 5405 5901 2035
Size: 75 2353 496 2336
Fragments arranged by size:
2353 2336 496 400 207 165 75 70
33 31 19 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2355 2572 1163
Size: 573 619 217 4793
Fragments arranged by size:
4793 619 573 217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3652 4413 5441 1540
Size: 275 186 1651 761 1028 2301
Fragments arranged by size:
2301 1651 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2526 3141 4263 4348 5419 2024
Size: 502 615 1122 85 1071 2807
Fragments arranged by size:
2807 1122 1071 615 502 85
BsmI GAATG_Cn'
Cuts at: 3713 3806 3713
Size: 93 6109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2228 2526 2873 2921 3069 3141
Size: 399 234 204 298 347 48 148 72
Cuts at: 3141 3521 4263 4348 5419 1391
Size: 380 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 347 298 234
204 148 85 72 48
BspEI T'CCGG_A
Cuts at: 5865 5865
Size: 6202
BspHI T'CATG_A
Cuts at: 4091 5821 4091
Size: 1730 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2791 3557 844
Size: 1331 616 766 3489
Fragments arranged by size:
3489 1331 766 616
BsrBI CCG'CTC
Cuts at: 1394 1823 3112 4089 1394
Size: 429 1289 977 3507
Fragments arranged by size:
3507 1289 977 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4697 4871 65
Size: 1183 3449 174 1396
Fragments arranged by size:
3449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4846 5033 1700
Size: 110 3036 187 2869
Fragments arranged by size:
3036 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6202
BssSI C'ACGA_G
Cuts at: 4262 5274 4262
Size: 1012 5190
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2804 2813 3187 794
Size: 2010 9 374 3809
Fragments arranged by size:
3809 2010 374 9
BstBI TT'CG_AA
Cuts at: 5006 5006
Size: 6202
BstEII G'GTnAC_C
Cuts at: 1184 2998 1184
Size: 1814 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 2954 2954
Size: 6202
BstYI r'GATC_y
Cuts at: 1972 3366 3532 3899 4284 4301 5753 5839 5851
Size: 1394 166 367 385 17 1452 86 12
Cuts at: 5851 6197 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1394 385 367 346 166 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 513
Size: 552 939 93 4618
Fragments arranged by size:
4618 939 552 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 487
Size: 683 56 896 4567
Fragments arranged by size:
4567 896 683 56
BtsI GCAGTG_nn'
Cuts at: 2811 2844 3718 4512 4532 2811
Size: 33 874 794 20 4481
Fragments arranged by size:
4481 874 794 33 20
ClaI AT'CG_AT
Cuts at: 1554 3249 1554
Size: 1695 4507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6167
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DraIII CAC_nnn'GTG
Cuts at: 3175 3175
Size: 6202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5209 809
Size: 507 3893 1802
Fragments arranged by size:
3893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2704 2841
Size: 54 1425 50 275 18 369 505 137
Cuts at: 2841 3036 3113 3424 3439 3649 4538 6167 8
Size: 195 77 311 15 210 889 1629 43
Fragments arranged by size:
1629 1425 889 505 369 311 275 210
195 137 77 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3649 1537
Size: 275 1837 4090
Fragments arranged by size:
4090 1837 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3081 4132 1353
Size: 1728 1051 3423
Fragments arranged by size:
3423 1728 1051
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 4789 5163 5309 387
Size: 1064 138 660 2540 374 146 1280
Fragments arranged by size:
2540 1280 1064 660 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2790 2976 3083 3477 4265 4862
Size: 393 428 730 186 107 394 788 597
Cuts at: 4862 5649 5917 1239
Size: 787 268 1524
Fragments arranged by size:
1524 788 787 730 597 428 394 393
268 186 107
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2078
Size: 6202
EcoO109I rG'GnC_Cy
Cuts at: 2071 2359 2530 2602 3415 2071
Size: 288 171 72 813 4858
Fragments arranged by size:
4858 813 288 171 72
EcoRI G'AATT_C
Cuts at: 3511 3889 3511
Size: 378 5824
FspI TGC'GCA
Cuts at: 3224 4708 3224
Size: 1484 4718
HaeII r_GCGC'y
Cuts at: 1678 2198 3165 5349 1678
Size: 520 967 2184 2531
Fragments arranged by size:
2531 2184 967 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 2660 2692 2812
Size: 32 135 32 592 32 464 32 120
Cuts at: 2812 2844 4843 4875 4917 4949 1373
Size: 32 1999 32 42 32 2626
Fragments arranged by size:
2626 1999 592 464 135 120 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3546 3792 669
Size: 988 281 1608 246 3079
Fragments arranged by size:
3079 1608 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6202
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 6202
KpnI G_GTAC'C
Cuts at: 2865 3527 2865
Size: 662 5540
MfeI C'AATT_G
Cuts at: 3801 3801
Size: 6202
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3071 5316 5500 1305
Size: 1766 2245 184 2007
Fragments arranged by size:
2245 2007 1766 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2706 3038 10
Size: 54 1425 343 369 505 332 3174
Fragments arranged by size:
3174 1425 505 369 343 332 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 2426 2510 2969 3176 4160 4519
Size: 648 1134 126 84 459 207 984 359
Cuts at: 4519 4678 518
Size: 159 2042
Fragments arranged by size:
2042 1134 984 648 459 359 207 159
126 84
MspA1I CmG'CkG
Cuts at: 1606 2185 2191 2377 2789 2801 2852 3429 3618
Size: 579 6 186 412 12 51 577 189
Cuts at: 3618 4295 5443 5688 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 577 412 245 189
186 51 12 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 2097 513
Size: 552 939 93 4618
Fragments arranged by size:
4618 939 552 93
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3649 3649
Size: 6202
NspI r_CATG'y
Cuts at: 1132 1229 2305 3560 5105 1132
Size: 97 1076 1255 1545 2229
Fragments arranged by size:
2229 1545 1255 1076 97
PasI CC'CwG_GG
Cuts at: 1348 2866 2914 1348
Size: 1518 48 4636
Fragments arranged by size:
4636 1518 48
PciI A'CATG_T
Cuts at: 2301 5101 2301
Size: 2800 3402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2914 1108
Size: 1806 4396
PfoI T'CCnGG_A
Cuts at: 3060 3060
Size: 6202
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 5784 5816 4271
Size: 32 1481 32 4657
Fragments arranged by size:
4657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2530 2602 2359
Size: 171 72 5959
Fragments arranged by size:
5959 171 72
PshAI GACnn'nnGTC
Cuts at: 1613 2884 1613
Size: 1271 4931
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PstI C_TGCA'G
Cuts at: 830 2172 2809 3554 830
Size: 1342 637 745 3478
Fragments arranged by size:
3478 1342 745 637
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6202
PvuII CAG'CTG
Cuts at: 2377 2801 2377
Size: 424 5778
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SanDI GG'GwC_CC
Cuts at: 2602 2602
Size: 6202
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 2711 4450 6162 2711
Size: 1739 1712 2751
Fragments arranged by size:
2751 1739 1712
SfcI C'TryA_G
Cuts at: 826 1045 2168 2805 3207 3550 4685 5366 5557
Size: 219 1123 637 402 343 1135 681 191
Cuts at: 5557 826
Size: 1471
Fragments arranged by size:
1471 1135 1123 681 637 402 343 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3413 3413
Size: 6202
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 2600 3529 1872
Size: 728 929 4545
Fragments arranged by size:
4545 929 728
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4306 5207 5469 820
Size: 197 926 2363 901 262 1553
Fragments arranged by size:
2363 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 3560 1132
Size: 2428 3774
SspI AAT'ATT
Cuts at: 5 52 4126 5
Size: 47 4074 2081
Fragments arranged by size:
4074 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 2567 2775 1517
Size: 750 300 208 4944
Fragments arranged by size:
4944 750 300 208
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2382 3240 3641 513
Size: 552 939 93 285 858 401 3074
Fragments arranged by size:
3074 939 858 552 401 285 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2690 4229 4246 4399 4584 5029 325
Size: 1255 1110 1539 17 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 1110 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2709 4448 6160
Size: 274 80 33 51 1443 732 1739 1712
Cuts at: 6160 96
Size: 138
Fragments arranged by size:
1739 1712 1443 732 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2838 2870 6162 6194 2838
Size: 32 3292 32 2846
Fragments arranged by size:
3292 2846 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2315 1316
Size: 999 5203
XbaI T'CTAG_A
Cuts at: 3538 3538
Size: 6202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 3010 3419 2170
Size: 840 409 4953
Fragments arranged by size:
4953 840 409
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6202
XmaI C'CCGG_G
Cuts at: 1870 2598 3527 1870
Size: 728 929 4545
Fragments arranged by size:
4545 929 728
XmnI GAAnn'nnTTC
Cuts at: 4331 4331
Size: 6202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfoI SrfI SwaI