Restriction map for
pFN21AB0472
(Circular) (Six-base) MAPSORT of: pFN21AB0472.seq Check: 3296 from: 1 to: 7684
pFN21AB0472 7684 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2582 4078 4700 4788 2582
Size: 1496 622 88 5478
Fragments arranged by size:
5478 1496 622 88
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5494 278
Size: 53 83 186 936 3958 2468
Fragments arranged by size:
3958 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5027 1937
Size: 3090 4594
Acc65I G'GTAC_C
Cuts at: 5005 5005
Size: 7684
AclI AA'CG_TT
Cuts at: 1563 5811 6184 1563
Size: 4248 373 3063
Fragments arranged by size:
4248 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 3014 3107 3194 4137 5747 7131 7399
Size: 536 846 93 87 943 1610 1384 268
Cuts at: 7399 1632
Size: 1917
Fragments arranged by size:
1917 1610 1384 943 846 536 268 93
87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7487
AflIII A'CryG_T
Cuts at: 1203 6583 7560 1203
Size: 5380 977 1327
Fragments arranged by size:
5380 1327 977
AgeI A'CCGG_T
Cuts at: 4400 6515 4400
Size: 2115 5569
AhdI GACnn_n'nnGTC
Cuts at: 3523 6413 3523
Size: 2890 4794
AleI CACnn'nnGTG
Cuts at: 1166 2672 1166
Size: 1506 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4244 1184
Size: 3060 4624
AlwNI CAG_nnn'CTG
Cuts at: 2094 3577 4070 5427 6999 2094
Size: 1483 493 1357 1572 2779
Fragments arranged by size:
2779 1572 1483 1357 493
ApaI G_GGCC'C
Cuts at: 4363 4906 4363
Size: 543 7141
ApaLI G'TGCA_C
Cuts at: 3662 4708 5741 6897 3662
Size: 1046 1033 1156 4449
Fragments arranged by size:
4449 1156 1046 1033
ApoI r'AATT_y
Cuts at: 1493 2398 4993 5219 5371 7480 1493
Size: 905 2595 226 152 2109 1697
Fragments arranged by size:
2595 2109 1697 905 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6238 160
Size: 872 5206 1606
Fragments arranged by size:
5206 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7684
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2325 3020 3098 3146 4371 4784
Size: 73 117 265 695 78 48 1225 413
Cuts at: 4784 5009 1870
Size: 225 4545
Fragments arranged by size:
4545 1225 695 413 265 225 117 78
73 48
BamHI G'GATC_C
Cuts at: 3162 4880 5014 5381 3162
Size: 1718 134 367 5465
Fragments arranged by size:
5465 1718 367 134
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3411 3669 5005 6460 618
Size: 325 217 627 1624 258 1336 1455 1842
Fragments arranged by size:
1842 1624 1455 1336 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2061 2124 2211 4363 4813 4906 4944 5003
Size: 670 63 87 2152 450 93 38 59
Cuts at: 5003 1391
Size: 4072
Fragments arranged by size:
4072 2152 670 450 93 87 63 59
38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3881 928
Size: 971 1982 4731
Fragments arranged by size:
4731 1982 971
BbvCI CC'TCA_GC
Cuts at: 3285 3780 4304 4769 3285
Size: 495 524 465 6200
Fragments arranged by size:
6200 524 495 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3289 3323 5875 5909 1903
Size: 34 1352 34 2552 34 3678
Fragments arranged by size:
3678 2552 1352 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2170 5576 6786 2170
Size: 3406 1210 3068
Fragments arranged by size:
3406 3068 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7684
BfrBI ATG'CAT
Cuts at: 3866 3866
Size: 7684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3149 4176 4679
Size: 107 122 71 631 605 1477 1027 503
Cuts at: 4679 4901 6295 136
Size: 222 1394 1525
Fragments arranged by size:
1525 1477 1394 1027 631 605 503 222
122 107 71
BglII A'GATC_T
Cuts at: 2522 4630 7679 2522
Size: 2108 3049 2527
Fragments arranged by size:
3049 2527 2108
BlpI GC'TnA_GC
Cuts at: 4943 5101 4943
Size: 158 7526
Bme1580I G_kGCm'C
Cuts at: 1790 2875 3139 3465 3666 3817 4363 4431 4712
Size: 1085 264 326 201 151 546 68 281
Cuts at: 4712 4906 5745 6901 1790
Size: 194 839 1156 2573
Fragments arranged by size:
2573 1156 1085 839 546 326 281 264
201 194 151 68
BmgBI CAC'GTC
Cuts at: 1318 3115 1318
Size: 1797 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 2235 2322
Size: 416 53 83 399 24 767 51 87
Cuts at: 2322 2526 2667 5415 6373 442
Size: 204 141 2748 958 1753
Fragments arranged by size:
2748 1753 958 767 416 399 204 141
87 83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7684
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 7684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2108 2840 3527 3617 4403 4529 6344
Size: 836 33 732 687 90 786 126 1815
Cuts at: 6344 1239
Size: 2579
Fragments arranged by size:
2579 1815 836 786 732 687 126 90
33
Bpu10I CC'TnA_GC
Cuts at: 3285 3675 3780 4304 4769 3285
Size: 390 105 524 465 6200
Fragments arranged by size:
6200 524 465 390 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5809 6674 6972 5809
Size: 865 298 6521
Fragments arranged by size:
6521 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3622 6347 882
Size: 617 2123 2725 2219
Fragments arranged by size:
2725 2219 2123 617
BsaAI yAC'GTr
Cuts at: 493 1204 3826 5475 493
Size: 711 2622 1649 2702
Fragments arranged by size:
2702 2622 1649 711
BsaBI GATnn'nnATC
Cuts at: 7678 7678
Size: 7684
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 4719 5491 5873
Size: 53 83 186 936 2287 899 772 382
Cuts at: 5873 6508 275
Size: 635 1451
Fragments arranged by size:
2287 1451 936 899 772 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3786 4028 4400 6117 6515 6789 6936 7347 3786
Size: 242 372 1717 398 274 147 411 4123
Fragments arranged by size:
4123 1717 411 398 372 274 242 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 2854 2884 1178
Size: 30 165 30 672 30 749 30 5978
Fragments arranged by size:
5978 749 672 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2485 3302 3524 3527 4307 4348 4658
Size: 910 389 817 222 3 780 41 310
Cuts at: 4658 4930 1186
Size: 272 3940
Fragments arranged by size:
3940 910 817 780 389 310 272 222
41 3
BseYI C'CCAG_C
Cuts at: 2488 3354 4097 4133 4390 4408 4891 4905 6887
Size: 866 743 36 257 18 483 14 1982
Cuts at: 6887 7383 2488
Size: 496 2789
Fragments arranged by size:
2789 1982 866 743 496 483 257 36
18 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3344 4487 1163
Size: 573 1608 1143 4360
Fragments arranged by size:
4360 1608 1143 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5134 5895 6923 1540
Size: 275 186 3133 761 1028 2301
Fragments arranged by size:
3133 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2124 2514 3666 4299 4712 4944 5745 5830
Size: 63 390 1152 633 413 232 801 85
Cuts at: 5830 6901 2061
Size: 1071 2844
Fragments arranged by size:
2844 1152 1071 801 633 413 390 232
85 63
BsmI GAATG_Cn'
Cuts at: 5195 5288 5195
Size: 93 7591
BsmBI CGTCTCn'nnnn_
Cuts at: 2061 3446 2061
Size: 1385 6299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2124 2211 2514 2875 3139 3465
Size: 399 271 63 87 303 361 264 326
Cuts at: 3465 3666 3817 4299 4363 4431 4712 4813 4906
Size: 201 151 482 64 68 281 101 93
Cuts at: 4906 4944 5003 5745 5830 6901 1391
Size: 38 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 361 326 303
281 271 264 201 151 101 93 87
85 68 64 63 59 38
BspEI T'CCGG_A
Cuts at: 7347 7347
Size: 7684
BspHI T'CATG_A
Cuts at: 5573 7303 5573
Size: 1730 5954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2079 2146 2582 3651 4018 4078 4700
Size: 1214 21 67 436 1069 367 60 622
Cuts at: 4700 4788 5039 844
Size: 88 251 3489
Fragments arranged by size:
3489 1214 1069 622 436 367 251 88
67 60 21
BsrBI CCG'CTC
Cuts at: 1394 1823 5571 1394
Size: 429 3748 3507
Fragments arranged by size:
3748 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3606 6179 6353 65
Size: 1183 2358 2573 174 1396
Fragments arranged by size:
2573 2358 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3485 4093 4400 4715 4859 6328 6515
Size: 110 1675 608 307 315 144 1469 187
Cuts at: 6515 1700
Size: 2869
Fragments arranged by size:
2869 1675 1469 608 315 307 187 144
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7684
BssHII G'CGCG_C
Cuts at: 1925 4533 1925
Size: 2608 5076
BssSI C'ACGA_G
Cuts at: 5744 6756 5744
Size: 1012 6672
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4070 794
Size: 3276 4408
BstBI TT'CG_AA
Cuts at: 2962 6488 2962
Size: 3526 4158
BstEII G'GTnAC_C
Cuts at: 1184 2197 2329 1184
Size: 1013 132 6539
Fragments arranged by size:
6539 1013 132
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 2174 2674 4204 2044
Size: 130 500 1530 5524
Fragments arranged by size:
5524 1530 500 130
BstYI r'GATC_y
Cuts at: 1972 2316 2522 3162 3746 4630 4788 4880 5014
Size: 344 206 640 584 884 158 92 134
Cuts at: 5014 5381 5766 5783 7235 7321 7333 7679 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 884 640 584 385 367 346
344 206 158 134 92 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3419 4570 3419
Size: 1151 6533
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 2641 2745 3402 4638 4792
Size: 552 939 222 415 104 657 1236 154
Cuts at: 4792 513
Size: 3405
Fragments arranged by size:
3405 1236 939 657 552 415 222 154
104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2725 487
Size: 683 56 1499 5446
Fragments arranged by size:
5446 1499 683 56
BtsI GCAGTG_nn'
Cuts at: 5200 5994 6014 5200
Size: 794 20 6870
Fragments arranged by size:
6870 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7649
DraI TTT'AAA
Cuts at: 4988 4988
Size: 7684
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3119 3941 6691 809
Size: 507 1803 822 2750 1802
Fragments arranged by size:
2750 1803 1802 822 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 4215 4623
Size: 54 1425 50 275 18 477 1908 408
Cuts at: 4623 5131 6020 7649 8
Size: 508 889 1629 43
Fragments arranged by size:
1908 1629 1425 889 508 477 408 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5131 1537
Size: 275 3319 4090
Fragments arranged by size:
4090 3319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 3403 3444 4325 5614 1353
Size: 888 1162 41 881 1289 3423
Fragments arranged by size:
3423 1289 1162 888 881 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3539 6271 6645 6791 387
Size: 1064 138 1950 2732 374 146 1280
Fragments arranged by size:
2732 1950 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 2122 4942 2059
Size: 63 2820 4801
Fragments arranged by size:
4801 2820 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2108 2168 2840 3014 3107 3194
Size: 393 443 33 60 672 174 93 87
Cuts at: 3194 3527 3617 4137 4403 4529 5747 6344 7131
Size: 333 90 520 266 126 1218 597 787
Cuts at: 7131 7399 1239
Size: 268 1524
Fragments arranged by size:
1524 1218 787 672 597 520 443 393
333 268 266 174 126 93 90 87
60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3173 3284 3173
Size: 111 7573
EcoO109I rG'GnC_Cy
Cuts at: 2091 3142 3424 4007 4359 4360 4385 4657 4849
Size: 1051 282 583 352 1 25 272 192
Cuts at: 4849 4902 2091
Size: 53 4873
Fragments arranged by size:
4873 1051 583 352 282 272 192 53
25 1
EcoRI G'AATT_C
Cuts at: 2398 4993 5371 2398
Size: 2595 378 4711
Fragments arranged by size:
4711 2595 378
EcoRV GAT'ATC
Cuts at: 2266 2890 2266
Size: 624 7060
FspI TGC'GCA
Cuts at: 4443 6190 4443
Size: 1747 5937
HaeII r_GCGC'y
Cuts at: 1678 2767 6831 1678
Size: 1089 4064 2531
Fragments arranged by size:
4064 2531 1089
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 3246 3278 3509
Size: 32 135 32 503 32 1139 32 231
Cuts at: 3509 3541 6325 6357 6399 6431 1373
Size: 32 2784 32 42 32 2626
Fragments arranged by size:
2784 2626 1139 503 231 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3856 5028 5274 669
Size: 988 281 1918 1172 246 3079
Fragments arranged by size:
3079 1918 1172 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7684
HpaI GTT'AAC
Cuts at: 5274 5274
Size: 7684
KpnI G_GTAC'C
Cuts at: 5009 5009
Size: 7684
MfeI C'AATT_G
Cuts at: 5283 5283
Size: 7684
MluI A'CGCG_T
Cuts at: 7560 7560
Size: 7684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2760 3774 4215 6798 6982 1305
Size: 1455 1014 441 2583 184 2007
Fragments arranged by size:
2583 2007 1455 1014 441 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 4217 10
Size: 54 1425 343 477 1908 3477
Fragments arranged by size:
3477 1908 1425 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 2672 2744 3611 4202 4346 5642
Size: 648 1341 165 72 867 591 144 1296
Cuts at: 5642 6001 6160 518
Size: 359 159 2042
Fragments arranged by size:
2042 1341 1296 867 648 591 359 165
159 144 72
MspA1I CmG'CkG
Cuts at: 1606 2018 2228 2305 2557 2785 3214 4039 4866
Size: 412 210 77 252 228 429 825 827
Cuts at: 4866 4909 5100 5777 6925 7170 1606
Size: 43 191 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 825 677 429 412 252
245 228 210 191 77 43
NaeI GCC'GGC
Cuts at: 3487 4095 4717 4861 3487
Size: 608 622 144 6310
Fragments arranged by size:
6310 622 608 144
NcoI C'CATG_G
Cuts at: 513 1065 2004 2641 4792 513
Size: 552 939 637 2151 3405
Fragments arranged by size:
3405 2151 939 637 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7684
NgoMIV G'CCGG_C
Cuts at: 3485 4093 4715 4859 3485
Size: 608 622 144 6310
Fragments arranged by size:
6310 622 608 144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7684
NotI GC'GGCC_GC
Cuts at: 5131 5131
Size: 7684
NsiI A_TGCA'T
Cuts at: 3868 3868
Size: 7684
NspI r_CATG'y
Cuts at: 1132 1229 3337 5042 6587 1132
Size: 97 2108 1705 1545 2229
Fragments arranged by size:
2229 2108 1705 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3973 4003 4381 4461 4729 1348
Size: 2625 30 378 80 268 4303
Fragments arranged by size:
4303 2625 378 268 80 30
PciI A'CATG_T
Cuts at: 6583 6583
Size: 7684
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3577 1108
Size: 2469 5215
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 7684
PmeI GTTT'AAAC
Cuts at: 4988 4988
Size: 7684
PmlI CAC'GTG
Cuts at: 3826 3826
Size: 7684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5753 5785 7266 7298 5753
Size: 32 1481 32 6139
Fragments arranged by size:
6139 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 3424 4007 4385 4849 2091
Size: 1333 583 378 464 4926
Fragments arranged by size:
4926 1333 583 464 378
PshAI GACnn'nnGTC
Cuts at: 1613 2327 1613
Size: 714 6970
PsiI TTA'TAA
Cuts at: 5254 5254
Size: 7684
PspOMI G'GGCC_C
Cuts at: 4359 4902 4359
Size: 543 7141
PspXI vC'TCGA_Gb
Cuts at: 3146 3146
Size: 7684
PstI C_TGCA'G
Cuts at: 830 2157 4066 4521 4754 4914 5036 830
Size: 1327 1909 455 233 160 122 3478
Fragments arranged by size:
3478 1909 1327 455 233 160 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7684
PvuII CAG'CTG
Cuts at: 2018 2305 3214 4909 2018
Size: 287 909 1695 4793
Fragments arranged by size:
4793 1695 909 287
SacI G_AGCT'C
Cuts at: 2061 2124 4944 2061
Size: 63 2820 4801
Fragments arranged by size:
4801 2820 63
SacII CC_GC'GG
Cuts at: 2229 2229
Size: 7684
SalI G'TCGA_C
Cuts at: 1936 5026 1936
Size: 3090 4594
SanDI GG'GwC_CC
Cuts at: 4385 4385
Size: 7684
SapI GCTCTTCn'nnn_
Cuts at: 2241 2241
Size: 7684
SbfI CC_TGCA'GG
Cuts at: 5036 5036
Size: 7684
ScaI AGT'ACT
Cuts at: 5932 7644 5932
Size: 1712 5972
SexAI A'CCwGG_T
Cuts at: 2907 2907
Size: 7684
SfcI C'TryA_G
Cuts at: 826 1045 2153 2404 4062 4517 4750 4910 5032
Size: 219 1108 251 1658 455 233 160 122
Cuts at: 5032 6167 6848 7039 826
Size: 1135 681 191 1471
Fragments arranged by size:
1658 1471 1135 1108 681 455 251 233
219 191 160 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7684
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7684
SmaI CCC'GGG
Cuts at: 1872 2327 5011 1872
Size: 455 2684 4545
Fragments arranged by size:
4545 2684 455
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 3020 3146 5788 6689 6951
Size: 197 926 117 960 126 2642 901 262
Cuts at: 6951 820
Size: 1553
Fragments arranged by size:
2642 1553 960 926 901 262 197 126
117
SnaBI TAC'GTA
Cuts at: 493 5475 493
Size: 4982 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7684
SphI G_CATG'C
Cuts at: 1132 3337 5042 1132
Size: 2205 1705 3774
Fragments arranged by size:
3774 2205 1705
SspI AAT'ATT
Cuts at: 5 52 5608 5
Size: 47 5556 2081
Fragments arranged by size:
5556 2081 47
StuI AGG'CCT
Cuts at: 1517 4568 1517
Size: 3051 4633
StyI C'CwwG_G
Cuts at: 513 1065 2004 2641 3376 3706 4792 5123 513
Size: 552 939 637 735 330 1086 331 3074
Fragments arranged by size:
3074 1086 939 735 637 552 331 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2332 2348 2406 5711 5728 5881 6066
Size: 1255 752 16 58 3305 17 153 185
Cuts at: 6066 6511 325
Size: 445 1498
Fragments arranged by size:
3305 1498 1255 752 445 185 153 58
17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5930 7642 96
Size: 274 80 33 51 1443 3953 1712 138
Fragments arranged by size:
3953 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2854 2886 7644 7676 2854
Size: 32 4758 32 2862
Fragments arranged by size:
4758 2862 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2909 1316
Size: 1593 6091
XbaI T'CTAG_A
Cuts at: 5020 5020
Size: 7684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2648 2743 2648
Size: 95 7589
XhoI C'TCGA_G
Cuts at: 1943 2060 3020 3146 1943
Size: 117 960 126 6481
Fragments arranged by size:
6481 960 126 117
XmaI C'CCGG_G
Cuts at: 1870 2325 5009 1870
Size: 455 2684 4545
Fragments arranged by size:
4545 2684 455
XmnI GAAnn'nnTTC
Cuts at: 5813 5813
Size: 7684
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5492 276
Size: 53 83 186 936 3958 2468
Fragments arranged by size:
3958 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
DraIII FalI FseI FspAI KasI NarI NruI PacI
PsrI RsrII SfiI SfoI SrfI SwaI