Restriction map for
pFN21AB0471
(Circular) (Six-base) MAPSORT of: pFN21AB0471.seq Check: 9832 from: 1 to: 6250
pFN21AB0471 6250 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4060 278
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2213 3593 1937
Size: 276 1380 4594
Fragments arranged by size:
4594 1380 276
Acc65I G'GTAC_C
Cuts at: 3571 3571
Size: 6250
AclI AA'CG_TT
Cuts at: 1563 2357 4377 4750 1563
Size: 794 2020 373 3063
Fragments arranged by size:
3063 2020 794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2017 3444 4313 5697 5965 1632
Size: 385 1427 869 1384 268 1917
Fragments arranged by size:
1917 1427 1384 869 385 268
AflII C'TTAA_G
Cuts at: 820 1017 2818 820
Size: 197 1801 4252
Fragments arranged by size:
4252 1801 197
AflIII A'CryG_T
Cuts at: 1203 2397 5149 6126 1203
Size: 1194 2752 977 1327
Fragments arranged by size:
2752 1327 1194 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 3032 3091 4979 3032
Size: 59 1888 4303
Fragments arranged by size:
4303 1888 59
AleI CACnn'nnGTG
Cuts at: 1166 2786 1166
Size: 1620 4630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6250
AlwNI CAG_nnn'CTG
Cuts at: 2726 3993 5565 2726
Size: 1267 1572 3411
Fragments arranged by size:
3411 1572 1267
ApaI G_GGCC'C
Cuts at: 3186 3186
Size: 6250
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 2143 2462 2578 2845 3559 3785 3937 6046
Size: 650 319 116 267 714 226 152 2109
Cuts at: 6046 1493
Size: 1697
Fragments arranged by size:
2109 1697 714 650 319 267 226 152
116
AseI AT'TA_AT
Cuts at: 160 1032 2448 4804 160
Size: 872 1416 2356 1606
Fragments arranged by size:
2356 1606 1416 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 3575 1870
Size: 73 1632 4545
Fragments arranged by size:
4545 1632 73
BamHI G'GATC_C
Cuts at: 3385 3580 3947 3385
Size: 195 367 5688
Fragments arranged by size:
5688 367 195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3571 5026 618
Size: 325 217 627 1784 1455 1842
Fragments arranged by size:
1842 1784 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3135 3186 3569 1391
Size: 1744 51 383 4072
Fragments arranged by size:
4072 1744 383 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5279
BbvCI CC'TCA_GC
Cuts at: 3159 3159
Size: 6250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4441 4475 1903
Size: 34 50 34 2420 34 3678
Fragments arranged by size:
3678 2420 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4142 5352 4142
Size: 1210 5040
BclI T'GATC_A
Cuts at: 1548 3493 1548
Size: 1945 4305
BfrBI ATG'CAT
Cuts at: 3022 3022
Size: 6250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4861 136
Size: 107 122 71 631 605 3189 1525
Fragments arranged by size:
3189 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6245 6245
Size: 6250
BlpI GC'TnA_GC
Cuts at: 3667 3667
Size: 6250
Bme1580I G_kGCm'C
Cuts at: 1790 3186 4311 5467 1790
Size: 1396 1125 1156 2573
Fragments arranged by size:
2573 1396 1156 1125
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3070 3981 4939
Size: 416 53 83 399 24 1653 911 958
Cuts at: 4939 442
Size: 1753
Fragments arranged by size:
1753 1653 958 911 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3194 4910 1239
Size: 1955 1716 2579
Fragments arranged by size:
2579 1955 1716
Bpu10I CC'TnA_GC
Cuts at: 3159 3159
Size: 6250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3196 4375 5240 5538 3196
Size: 1179 865 298 3908
Fragments arranged by size:
3908 1179 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4913 882
Size: 617 3414 2219
Fragments arranged by size:
3414 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4041 493
Size: 711 2837 2702
Fragments arranged by size:
2837 2702 711
BsaBI GATnn'nnATC
Cuts at: 6244 6244
Size: 6250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4057 4439 5074 275
Size: 53 83 186 936 2524 382 635 1451
Fragments arranged by size:
2524 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2148 4683 5081 5355 5502 5913 2148
Size: 2535 398 274 147 411 2485
Fragments arranged by size:
2535 2485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3187 3217 3224 3254 1178
Size: 30 165 30 1784 30 7 30 4174
Fragments arranged by size:
4174 1784 165 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3113 3116 3208 1186
Size: 1927 3 92 4228
Fragments arranged by size:
4228 1927 92 3
BseYI C'CCAG_C
Cuts at: 3449 5453 5949 3449
Size: 2004 496 3750
Fragments arranged by size:
3750 2004 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5677
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 4461 5489 1540
Size: 275 186 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 3135 4311 4396 5467 2646
Size: 489 1176 85 1071 3429
Fragments arranged by size:
3429 1176 1071 489 85
BsmI GAATG_Cn'
Cuts at: 2026 3761 3854 2026
Size: 1735 93 4422
Fragments arranged by size:
4422 1735 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3360 3360
Size: 6250
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2646 3135 3186 3569 4311 4396 5467
Size: 399 856 489 51 383 742 85 1071
Cuts at: 5467 1391
Size: 2174
Fragments arranged by size:
2174 1071 856 742 489 399 383 85
51
BspEI T'CCGG_A
Cuts at: 5913 5913
Size: 6250
BspHI T'CATG_A
Cuts at: 2220 4139 5869 2220
Size: 1919 1730 2601
Fragments arranged by size:
2601 1919 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2889 3120 3605 844
Size: 2045 231 485 3489
Fragments arranged by size:
3489 2045 485 231
BsrBI CCG'CTC
Cuts at: 1394 1823 4137 1394
Size: 429 2314 3507
Fragments arranged by size:
3507 2314 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2135 2204 4745 4919 65
Size: 1183 887 69 2541 174 1396
Fragments arranged by size:
2541 1396 1183 887 174 69
BsrFI r'CCGG_y
Cuts at: 1700 1810 4894 5081 1700
Size: 110 3084 187 2869
Fragments arranged by size:
3084 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6250
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6250
BssSI C'ACGA_G
Cuts at: 2072 2312 4310 5322 2072
Size: 240 1998 1012 3000
Fragments arranged by size:
3000 1998 1012 240
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2726 794
Size: 1932 4318
BstBI TT'CG_AA
Cuts at: 5054 5054
Size: 6250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6250
BstXI CCAn_nnnn'nTGG
Cuts at: 2905 2905
Size: 6250
BstYI r'GATC_y
Cuts at: 1972 3385 3580 3947 4332 4349 5801 5887 5899
Size: 1413 195 367 385 17 1452 86 12
Cuts at: 5899 6245 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1413 385 367 346 195 86
17 12
BstZ17I GTA'TAC
Cuts at: 2214 2214
Size: 6250
Bsu36I CC'TnA_GG
Cuts at: 3117 3117
Size: 6250
BtgI C'CryG_G
Cuts at: 513 1065 2898 3243 513
Size: 552 1833 345 3520
Fragments arranged by size:
3520 1833 552 345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5511
Fragments arranged by size:
5511 683 56
BtsI GCAGTG_nn'
Cuts at: 3766 4560 4580 3766
Size: 794 20 5436
Fragments arranged by size:
5436 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6215
DraI TTT'AAA
Cuts at: 3554 3554
Size: 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5257 809
Size: 507 3941 1802
Fragments arranged by size:
3941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 2701 2867
Size: 54 1425 50 275 18 558 313 166
Cuts at: 2867 3417 3697 4586 6215 8
Size: 550 280 889 1629 43
Fragments arranged by size:
1629 1425 889 558 550 313 280 275
166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3697 1537
Size: 275 1885 4090
Fragments arranged by size:
4090 1885 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3222 3360 3463 4180 1353
Size: 1869 138 103 717 3423
Fragments arranged by size:
3423 1869 717 138 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4837 5211 5357 387
Size: 1064 138 3248 374 146 1280
Fragments arranged by size:
3248 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3133 3133
Size: 6250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 3194 3444 4313 4910 5697 5965
Size: 393 385 1177 250 869 597 787 268
Cuts at: 5965 1239
Size: 1524
Fragments arranged by size:
1524 1177 869 787 597 393 385 268
250
EcoNI CCTnn'n_nnAGG
Cuts at: 3116 3190 3116
Size: 74 6176
EcoO109I rG'GnC_Cy
Cuts at: 3183 3214 3466 3183
Size: 31 252 5967
Fragments arranged by size:
5967 252 31
EcoRI G'AATT_C
Cuts at: 3559 3937 3559
Size: 378 5872
EcoRV GAT'ATC
Cuts at: 2133 2133
Size: 6250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 3522 2692
Size: 830 5420
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 2993 5397 1678
Size: 1315 2404 2531
Fragments arranged by size:
2531 2404 1315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3018 3050 3077 3109 3187
Size: 32 135 32 1446 32 27 32 78
Cuts at: 3187 3219 3224 3256 3272 3304 3473 3505 4891
Size: 32 5 32 16 32 169 32 1386
Cuts at: 4891 4923 4965 4997 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1446 1386 169 135 78 42 32
32 32 32 32 32 32 32 32
32 27 16 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3376 3523 3594 3840 669
Size: 988 281 1438 147 71 246 3079
Fragments arranged by size:
3079 1438 988 281 246 147 71
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6250
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KpnI G_GTAC'C
Cuts at: 3575 3575
Size: 6250
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2871 3147 5364 5548 1305
Size: 1566 276 2217 184 2007
Fragments arranged by size:
2217 2007 1566 276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2390 2703 2869 3419 10
Size: 54 1425 343 558 313 166 550 2841
Fragments arranged by size:
2841 1425 558 550 343 313 166 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 2448 2450 2649 2786 2797 2903
Size: 648 1230 52 2 199 137 11 106
Cuts at: 2903 4208 4567 4726 518
Size: 1305 359 159 2042
Fragments arranged by size:
2042 1305 1230 648 359 199 159 137
106 52 11 2
MspA1I CmG'CkG
Cuts at: 1606 2346 3423 3666 4343 5491 5736 1606
Size: 740 1077 243 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 740 677 245 243
NcoI C'CATG_G
Cuts at: 513 1065 2898 513
Size: 552 1833 3865
Fragments arranged by size:
3865 1833 552
NdeI CA'TA_TG
Cuts at: 387 2089 387
Size: 1702 4548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NsiI A_TGCA'T
Cuts at: 3024 3024
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 2401 3608 5153 1132
Size: 97 1172 1207 1545 2229
Fragments arranged by size:
2229 1545 1207 1172 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6250
PciI A'CATG_T
Cuts at: 2397 5149 2397
Size: 2752 3498
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3415 1108
Size: 2307 3943
PfoI T'CCnGG_A
Cuts at: 3210 3210
Size: 6250
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 5832 5864 4319
Size: 32 1481 32 4705
Fragments arranged by size:
4705 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3214 3214
Size: 6250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 2952 3820 2952
Size: 868 5382
PspOMI G'GGCC_C
Cuts at: 3182 3182
Size: 6250
PstI C_TGCA'G
Cuts at: 830 3602 830
Size: 2772 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
SacI G_AGCT'C
Cuts at: 3135 3135
Size: 6250
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 4498 6210 4498
Size: 1712 4538
SfcI C'TryA_G
Cuts at: 826 1045 3066 3235 3598 4733 5414 5605 826
Size: 219 2021 169 363 1135 681 191 1471
Fragments arranged by size:
2021 1471 1135 681 363 219 191 169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2818 3175 4354 5255 5517 820
Size: 197 926 875 357 1179 901 262 1553
Fragments arranged by size:
1553 1179 926 901 875 357 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6250
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 4174 5
Size: 47 4122 2081
Fragments arranged by size:
4122 2081 47
StuI AGG'CCT
Cuts at: 1517 2318 3040 3053 1517
Size: 801 722 13 4714
Fragments arranged by size:
4714 801 722 13
StyI C'CwwG_G
Cuts at: 513 1065 2898 3526 3689 513
Size: 552 1833 628 163 3074
Fragments arranged by size:
3074 1833 628 552 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4277 4294 4447 4632 5077 325
Size: 1255 2697 17 153 185 445 1498
Fragments arranged by size:
2697 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4496 6208 96
Size: 274 80 33 51 1443 2519 1712 138
Fragments arranged by size:
2519 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2677 2709 6210 6242 2677
Size: 32 3501 32 2685
Fragments arranged by size:
3501 2685 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3586 3586
Size: 6250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2905 2905
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 2024 4379 2024
Size: 2355 3895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4058 276
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI DraIII FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI