Restriction map for
pFN21AB0468
(Circular) (Six-base) MAPSORT of: pFN21AB0468.seq Check: 2304 from: 1 to: 7657
pFN21AB0468 7657 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5467 278
Size: 53 83 186 936 3931 2468
Fragments arranged by size:
3931 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5000 1937
Size: 3063 4594
Acc65I G'GTAC_C
Cuts at: 4978 4978
Size: 7657
AclI AA'CG_TT
Cuts at: 1563 5784 6157 1563
Size: 4221 373 3063
Fragments arranged by size:
4221 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2210 3010 3819 3968 4559 4880 5720
Size: 423 155 800 809 149 591 321 840
Cuts at: 5720 7104 7372 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 840 809 800 591 423 321
268 155 149
AfeI AGC'GCT
Cuts at: 4203 4203
Size: 7657
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7460
AflIII A'CryG_T
Cuts at: 1203 6556 7533 1203
Size: 5353 977 1327
Fragments arranged by size:
5353 1327 977
AgeI A'CCGG_T
Cuts at: 6488 6488
Size: 7657
AhdI GACnn_n'nnGTC
Cuts at: 3703 6386 3703
Size: 2683 4974
AleI CACnn'nnGTG
Cuts at: 1166 2841 1166
Size: 1675 5982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2772 4548 1184
Size: 1588 1776 4293
Fragments arranged by size:
4293 1776 1588
AlwNI CAG_nnn'CTG
Cuts at: 2303 5400 6972 2303
Size: 3097 1572 2988
Fragments arranged by size:
3097 2988 1572
ApaLI G'TGCA_C
Cuts at: 2678 5714 6870 2678
Size: 3036 1156 3465
Fragments arranged by size:
3465 3036 1156
ApoI r'AATT_y
Cuts at: 1493 2429 3256 3417 3553 4009 4823 4966 5192
Size: 936 827 161 136 456 814 143 226
Cuts at: 5192 5344 7453 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 936 827 814 456 226 161
152 143 136
AseI AT'TA_AT
Cuts at: 160 1032 6211 160
Size: 872 5179 1606
Fragments arranged by size:
5179 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7657
AvaI C'yCGr_G
Cuts at: 1870 1943 4982 1870
Size: 73 3039 4545
Fragments arranged by size:
4545 3039 73
BamHI G'GATC_C
Cuts at: 3056 4987 5354 3056
Size: 1931 367 5359
Fragments arranged by size:
5359 1931 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3044 3317 4978 6433 618
Size: 325 217 627 1257 273 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 1257 627 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2516 2780 4976 1391
Size: 1125 264 2196 4072
Fragments arranged by size:
4072 2196 1125 264
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3986 928
Size: 971 2087 4599
Fragments arranged by size:
4599 2087 971
BbvCI CC'TCA_GC
Cuts at: 4179 4179
Size: 7657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5848 5882 1903
Size: 34 3911 34 3678
Fragments arranged by size:
3911 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3735 5549 6759 3735
Size: 1814 1210 4633
Fragments arranged by size:
4633 1814 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7657
BfrBI ATG'CAT
Cuts at: 4525 4798 4525
Size: 273 7384
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6268 136
Size: 107 122 71 631 605 4596 1525
Fragments arranged by size:
4596 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7652 7652
Size: 7657
BlpI GC'TnA_GC
Cuts at: 4318 5074 4318
Size: 756 6901
Bme1580I G_kGCm'C
Cuts at: 1790 2682 3438 5718 6874 1790
Size: 892 756 2280 1156 2573
Fragments arranged by size:
2573 2280 1156 892 756
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 3467 5388
Size: 416 53 83 399 24 1661 389 1921
Cuts at: 5388 6346 442
Size: 958 1753
Fragments arranged by size:
1921 1753 1661 958 416 399 389 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2769 1056
Size: 1713 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2725 2838 2845 4471 6317 1239
Size: 1486 113 7 1626 1846 2579
Fragments arranged by size:
2579 1846 1626 1486 113 7
Bpu10I CC'TnA_GC
Cuts at: 4179 4179
Size: 7657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2169 2506 2773 2905 3096 3576 4409 4465 5782
Size: 337 267 132 191 480 833 56 1317
Cuts at: 5782 6647 6945 2169
Size: 865 298 2881
Fragments arranged by size:
2881 1317 865 833 480 337 298 267
191 132 56
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 3618 6320 882
Size: 617 504 1615 2702 2219
Fragments arranged by size:
2702 2219 1615 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 5448 493
Size: 711 4244 2702
Fragments arranged by size:
4244 2702 711
BsaBI GATnn'nnATC
Cuts at: 3723 7651 3723
Size: 3928 3729
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2529 5464 5846 6481
Size: 53 83 186 936 996 2935 382 635
Cuts at: 6481 275
Size: 1451
Fragments arranged by size:
2935 1451 996 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2159 3727 4950 6090 6488 6762 6909 7320 2159
Size: 1568 1223 1140 398 274 147 411 2496
Fragments arranged by size:
2496 1568 1223 1140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7432
Fragments arranged by size:
7432 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2694 2772 4076 1186
Size: 1508 78 1304 4767
Fragments arranged by size:
4767 1508 1304 78
BseYI C'CCAG_C
Cuts at: 6860 7356 6860
Size: 496 7161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2742 1163
Size: 573 1006 6078
Fragments arranged by size:
6078 1006 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3617 5107 5868 6896 1540
Size: 275 186 1616 1490 761 1028 2301
Fragments arranged by size:
2301 1616 1490 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2516 2682 2930 3112 3592 4021 5718 5803 6874
Size: 166 248 182 480 429 1697 85 1071
Cuts at: 6874 2516
Size: 3299
Fragments arranged by size:
3299 1697 1071 480 429 248 182 166
85
BsmI GAATG_Cn'
Cuts at: 3304 5168 5261 3304
Size: 1864 93 5700
Fragments arranged by size:
5700 1864 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2516 2682 2780 2930 3112 3438 3592
Size: 399 726 166 98 150 182 326 154
Cuts at: 3592 4021 4976 5718 5803 6874 1391
Size: 429 955 742 85 1071 2174
Fragments arranged by size:
2174 1071 955 742 726 429 399 326
182 166 154 150 98 85
BspEI T'CCGG_A
Cuts at: 7320 7320
Size: 7657
BspHI T'CATG_A
Cuts at: 2566 4617 5546 7276 2566
Size: 2051 929 1730 2947
Fragments arranged by size:
2947 2051 1730 929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4824 5012 844
Size: 3980 188 3489
Fragments arranged by size:
3980 3489 188
BsrBI CCG'CTC
Cuts at: 1394 1823 4939 5544 1394
Size: 429 3116 605 3507
Fragments arranged by size:
3507 3116 605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2776 3065 4884 6152 6326 65
Size: 1183 1528 289 1819 1268 174 1396
Fragments arranged by size:
1819 1528 1396 1268 1183 289 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6301 6488 1700
Size: 110 4491 187 2869
Fragments arranged by size:
4491 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7657
BssHII G'CGCG_C
Cuts at: 1925 2536 1925
Size: 611 7046
BssSI C'ACGA_G
Cuts at: 4058 4197 5717 6729 4058
Size: 139 1520 1012 4986
Fragments arranged by size:
4986 1520 1012 139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7657
BstBI TT'CG_AA
Cuts at: 2945 6461 2945
Size: 3516 4141
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7657
BstXI CCAn_nnnn'nTGG
Cuts at: 2992 4786 2992
Size: 1794 5863
BstYI r'GATC_y
Cuts at: 1972 2250 2885 2960 2996 3056 3788 4987 5354
Size: 278 635 75 36 60 732 1199 367
Cuts at: 5354 5739 5756 7208 7294 7306 7652 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1199 732 635 385 367 346
278 86 75 60 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2316 513
Size: 552 939 312 5854
Fragments arranged by size:
5854 939 552 312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4120 487
Size: 683 56 2894 4024
Fragments arranged by size:
4024 2894 683 56
BtsI GCAGTG_nn'
Cuts at: 2836 2848 3033 3900 5173 5967 5987 2836
Size: 12 185 867 1273 794 20 4506
Fragments arranged by size:
4506 1273 867 794 185 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7657
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4866 4901 529
Size: 35 4302 35 3285
Fragments arranged by size:
4302 3285 35 35
DraI TTT'AAA
Cuts at: 3328 4375 4961 3328
Size: 1047 586 6024
Fragments arranged by size:
6024 1047 586
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2061 6664 809
Size: 507 745 4603 1802
Fragments arranged by size:
4603 1802 745 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2446 3172 5104
Size: 54 1425 50 275 18 616 726 1932
Cuts at: 5104 5993 7622 8
Size: 889 1629 43
Fragments arranged by size:
1932 1629 1425 889 726 616 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5104 1537
Size: 275 3292 4090
Fragments arranged by size:
4090 3292 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4509 5587 1353
Size: 3156 1078 3423
Fragments arranged by size:
3423 3156 1078
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6244 6618 6764 387
Size: 1064 138 4655 374 146 1280
Fragments arranged by size:
4655 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2514 2514
Size: 7657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2210 2725 2838 2845 3010 3819
Size: 393 423 155 515 113 7 165 809
Cuts at: 3819 3968 4471 4559 4880 5720 6317 7104 7372
Size: 149 503 88 321 840 597 787 268
Cuts at: 7372 1239
Size: 1524
Fragments arranged by size:
1524 840 809 787 597 515 503 423
393 321 268 165 155 149 113 88
7
EcoO109I rG'GnC_Cy
Cuts at: 2099 3118 2099
Size: 1019 6638
EcoRI G'AATT_C
Cuts at: 3553 4823 4966 5344 3553
Size: 1270 143 378 5866
Fragments arranged by size:
5866 1270 378 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3783 3783
Size: 7657
FspI TGC'GCA
Cuts at: 6163 6163
Size: 7657
HaeII r_GCGC'y
Cuts at: 1678 4205 6804 1678
Size: 2527 2599 2531
Fragments arranged by size:
2599 2531 2527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 3574 3606 3689
Size: 32 135 32 695 32 1275 32 83
Cuts at: 3689 3721 4578 4610 6298 6330 6372 6404 1373
Size: 32 857 32 1688 32 42 32 2626
Fragments arranged by size:
2626 1688 1275 857 695 135 83 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 4162 5001 5247 669
Size: 988 281 205 2019 839 246 3079
Fragments arranged by size:
3079 2019 988 839 281 246 205
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7657
HpaI GTT'AAC
Cuts at: 5247 5247
Size: 7657
KpnI G_GTAC'C
Cuts at: 4982 4982
Size: 7657
MfeI C'AATT_G
Cuts at: 5256 5256
Size: 7657
MluI A'CGCG_T
Cuts at: 7533 7533
Size: 7657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3362 3661 6771 6955 1305
Size: 2057 299 3110 184 2007
Fragments arranged by size:
3110 2057 2007 299 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2448 3174 10
Size: 54 1425 343 616 726 4493
Fragments arranged by size:
4493 1425 726 616 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2841 4616 5615 5974 6133 518
Size: 648 1675 1775 999 359 159 2042
Fragments arranged by size:
2042 1775 1675 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2422 3465 3870 4906 5073 5750 6898 7143
Size: 816 1043 405 1036 167 677 1148 245
Cuts at: 7143 1606
Size: 2120
Fragments arranged by size:
2120 1148 1043 1036 816 677 405 245
167
NcoI C'CATG_G
Cuts at: 513 1065 2004 2316 513
Size: 552 939 312 5854
Fragments arranged by size:
5854 939 552 312
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7657
NheI G'CTAG_C
Cuts at: 1052 2765 1052
Size: 1713 5944
NotI GC'GGCC_GC
Cuts at: 5104 5104
Size: 7657
NsiI A_TGCA'T
Cuts at: 4527 4800 4527
Size: 273 7384
NspI r_CATG'y
Cuts at: 1132 1229 2715 2765 5015 6560 1132
Size: 97 1486 50 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 1486 97 50
PasI CC'CwG_GG
Cuts at: 1348 2095 2695 1348
Size: 747 600 6310
Fragments arranged by size:
6310 747 600
PciI A'CATG_T
Cuts at: 6556 6556
Size: 7657
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4655 1108
Size: 3547 4110
PfoI T'CCnGG_A
Cuts at: 4366 4366
Size: 7657
PmeI GTTT'AAAC
Cuts at: 4961 4961
Size: 7657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5726 5758 7239 7271 5726
Size: 32 1481 32 6112
Fragments arranged by size:
6112 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2099 3118 2099
Size: 1019 6638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7657
PsiI TTA'TAA
Cuts at: 5227 5227
Size: 7657
PstI C_TGCA'G
Cuts at: 830 2843 4593 5009 830
Size: 2013 1750 416 3478
Fragments arranged by size:
3478 2013 1750 416
PvuI CG_AT'CG
Cuts at: 2001 3617 2001
Size: 1616 6041
PvuII CAG'CTG
Cuts at: 2422 3870 4906 2422
Size: 1448 1036 5173
Fragments arranged by size:
5173 1448 1036
SacI G_AGCT'C
Cuts at: 2516 2516
Size: 7657
SalI G'TCGA_C
Cuts at: 1936 4999 1936
Size: 3063 4594
SbfI CC_TGCA'GG
Cuts at: 5009 5009
Size: 7657
ScaI AGT'ACT
Cuts at: 2182 2989 5905 7617 2182
Size: 807 2916 1712 2222
Fragments arranged by size:
2916 2222 1712 807
SexAI A'CCwGG_T
Cuts at: 4803 4803
Size: 7657
SfcI C'TryA_G
Cuts at: 826 1045 2103 2304 2839 3004 3577 4589 5005
Size: 219 1058 201 535 165 573 1012 416
Cuts at: 5005 6140 6821 7012 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1058 1012 681 573 535 416
219 201 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7657
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7657
SmaI CCC'GGG
Cuts at: 1872 4984 1872
Size: 3112 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2184 2485 2788 2920 3111 3591
Size: 197 926 241 301 303 132 191 480
Cuts at: 3591 4388 4444 5761 6662 6924 820
Size: 797 56 1317 901 262 1553
Fragments arranged by size:
1553 1317 926 901 797 480 303 301
262 241 197 191 132 56
SnaBI TAC'GTA
Cuts at: 493 5448 493
Size: 4955 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7657
SphI G_CATG'C
Cuts at: 1132 5015 1132
Size: 3883 3774
SspI AAT'ATT
Cuts at: 5 52 2911 3184 5581 5
Size: 47 2859 273 2397 2081
Fragments arranged by size:
2859 2397 2081 273 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7657
StyI C'CwwG_G
Cuts at: 513 1065 2004 2316 4911 5096 513
Size: 552 939 312 2595 185 3074
Fragments arranged by size:
3074 2595 939 552 312 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 2708 4147 5684 5701 5854 6039
Size: 1255 549 579 1439 1537 17 153 185
Cuts at: 6039 6484 325
Size: 445 1498
Fragments arranged by size:
1537 1498 1439 1255 579 549 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2180 2987 3062
Size: 274 80 33 51 1443 203 807 75
Cuts at: 3062 3192 5903 7615 96
Size: 130 2711 1712 138
Fragments arranged by size:
2711 1712 1443 807 274 203 138 130
80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 3417 3449 3459 3491 3632 3664 7617
Size: 32 1343 32 10 32 141 32 3953
Cuts at: 7617 7649 2042
Size: 32 2050
Fragments arranged by size:
3953 2050 1343 141 32 32 32 32
32 10
Tth111I GACn'n_nGTC
Cuts at: 1316 4502 1316
Size: 3186 4471
XbaI T'CTAG_A
Cuts at: 2041 3707 4993 2041
Size: 1666 1286 4705
Fragments arranged by size:
4705 1666 1286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3581 4801 3581
Size: 1220 6437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7657
XmaI C'CCGG_G
Cuts at: 1870 4982 1870
Size: 3112 4545
XmnI GAAnn'nnTTC
Cuts at: 3741 3778 5786 3741
Size: 37 2008 5612
Fragments arranged by size:
5612 2008 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5465 276
Size: 53 83 186 936 3931 2468
Fragments arranged by size:
3931 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI