Restriction map for
pFN21AB0465
(Circular) (Six-base) MAPSORT of: pFN21AB0465.seq Check: 9077 from: 1 to: 6139
pFN21AB0465 6139 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2199 3949 278
Size: 53 83 186 936 663 1750 2468
Fragments arranged by size:
2468 1750 936 663 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2925 3482 1937
Size: 988 557 4594
Fragments arranged by size:
4594 988 557
Acc65I G'GTAC_C
Cuts at: 2259 3460 2259
Size: 1201 4938
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 4202 5586 5854 1632
Size: 809 1761 1384 268 1917
Fragments arranged by size:
1917 1761 1384 809 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 2412 5038 6015 1203
Size: 1209 2626 977 1327
Fragments arranged by size:
2626 1327 1209 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2963 1184
Size: 1779 4360
AlwNI CAG_nnn'CTG
Cuts at: 2029 2242 2349 3268 3882 5454 2029
Size: 213 107 919 614 1572 2714
Fragments arranged by size:
2714 1572 919 614 213 107
ApaLI G'TGCA_C
Cuts at: 2993 4196 5352 2993
Size: 1203 1156 3780
Fragments arranged by size:
3780 1203 1156
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 3196 3228 3321 3464 1870
Size: 73 1253 32 93 143 4545
Fragments arranged by size:
4545 1253 143 93 73 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2169 2202 2169
Size: 33 6106
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 3460 4915 618
Size: 325 217 627 472 1201 1455 1842
Fragments arranged by size:
1842 1455 1201 627 472 325 217
BanII G_rGCy'C
Cuts at: 1391 3197 3344 3458 1391
Size: 1806 147 114 4072
Fragments arranged by size:
4072 1806 147 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2602 2854 928
Size: 971 703 252 4213
Fragments arranged by size:
4213 971 703 252
BbvCI CC'TCA_GC
Cuts at: 2108 2108
Size: 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4330 4364 1903
Size: 34 50 34 2309 34 3678
Fragments arranged by size:
3678 2309 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2197 4031 5241 2197
Size: 1834 1210 3095
Fragments arranged by size:
3095 1834 1210
BclI T'GATC_A
Cuts at: 1548 2868 1548
Size: 1320 4819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3028 3242 4750
Size: 107 122 71 631 605 1356 214 1508
Cuts at: 4750 136
Size: 1525
Fragments arranged by size:
1525 1508 1356 631 605 214 122 107
71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3139 3556 3139
Size: 417 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2997 4200 5356 1790
Size: 1207 1203 1156 2573
Fragments arranged by size:
2573 1207 1203 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3870 4828 442
Size: 416 53 83 399 24 2453 958 1753
Fragments arranged by size:
2453 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3383 1056
Size: 2327 3812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 3289 2842
Size: 447 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2273 3068 4799 1239
Size: 949 85 795 1731 2579
Fragments arranged by size:
2579 1731 949 795 85
Bpu10I CC'TnA_GC
Cuts at: 2108 2108
Size: 6139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2830 2963 4802 882
Size: 617 1331 133 1839 2219
Fragments arranged by size:
2219 1839 1331 617 133
BsaAI yAC'GTr
Cuts at: 493 1204 2413 3930 493
Size: 711 1209 1517 2702
Fragments arranged by size:
2702 1517 1209 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2196 3946 4328 4963
Size: 53 83 186 936 663 1750 382 635
Cuts at: 4963 275
Size: 1451
Fragments arranged by size:
1750 1451 936 663 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5914
Fragments arranged by size:
5914 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2092 2834 2901 1186
Size: 847 59 742 67 4424
Fragments arranged by size:
4424 847 742 67 59
BseYI C'CCAG_C
Cuts at: 2638 2759 3103 5342 5838 2638
Size: 121 344 2239 496 2939
Fragments arranged by size:
2939 2239 496 344 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 1163
Size: 573 789 4777
Fragments arranged by size:
4777 789 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2997 4200 4285 5356 2997
Size: 1203 85 1071 3780
Fragments arranged by size:
3780 1203 1071 85
BsiWI C'GTAC_G
Cuts at: 2409 2409
Size: 6139
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2997 3197 3344 3458 4200 4285 5356
Size: 399 1207 200 147 114 742 85 1071
Cuts at: 5356 1391
Size: 2174
Fragments arranged by size:
2174 1207 1071 742 399 200 147 114
85
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 2613 4028 5758 2613
Size: 1415 1730 2994
Fragments arranged by size:
2994 1730 1415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2808 3015 3384 3494 844
Size: 1964 207 369 110 3489
Fragments arranged by size:
3489 1964 369 207 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2102 4026 1394
Size: 429 279 1924 3507
Fragments arranged by size:
3507 1924 429 279
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2262 2721 2890 4783 4970 1700
Size: 110 452 459 169 1893 187 2869
Fragments arranged by size:
2869 1893 459 452 187 169 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 3007 3019 794
Size: 1235 978 12 3914
Fragments arranged by size:
3914 1235 978 12
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 3306 3469 3836 4221 4238 5690 5776 5788
Size: 1334 163 367 385 17 1452 86 12
Cuts at: 5788 6134 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1334 385 367 346 163 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 3074 2301
Size: 773 5366
BtgI C'CryG_G
Cuts at: 513 1065 2337 3144 513
Size: 552 1272 807 3508
Fragments arranged by size:
3508 1272 807 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5400
Fragments arranged by size:
5400 683 56
BtsI GCAGTG_nn'
Cuts at: 2895 3154 3655 4449 4469 2895
Size: 259 501 794 20 4565
Fragments arranged by size:
4565 794 501 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2201 2606 5146 809
Size: 507 885 405 2540 1802
Fragments arranged by size:
2540 1802 885 507 405
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2892 2964 3586
Size: 54 1425 50 275 18 1062 72 622
Cuts at: 3586 4475 6104 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1062 889 622 275 72 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 3185 4069 1353
Size: 1385 447 884 3423
Fragments arranged by size:
3423 1385 884 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2708 4726 5100 5246 387
Size: 1064 138 1119 2018 374 146 1280
Fragments arranged by size:
2018 1280 1119 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2273 2441 3068 4202 4799 5586
Size: 393 556 85 168 627 1134 597 787
Cuts at: 5586 5854 1239
Size: 268 1524
Fragments arranged by size:
1524 1134 787 627 597 556 393 268
168 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 6139
EcoO109I rG'GnC_Cy
Cuts at: 2353 3099 3173 2353
Size: 746 74 5319
Fragments arranged by size:
5319 746 74
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2030 2578 5286 1678
Size: 352 548 2708 2531
Fragments arranged by size:
2708 2531 548 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3122 3154 4780 4812 4854
Size: 32 135 32 1550 32 1626 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 1626 1550 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3483 3729 669
Size: 988 281 1149 396 246 3079
Fragments arranged by size:
3079 1149 988 396 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 2263 3464 2263
Size: 1201 4938
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 2622 5253 5437 1305
Size: 792 525 2631 184 2007
Fragments arranged by size:
2631 2007 792 525 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4317
Fragments arranged by size:
4317 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 4097 4456 4615 518
Size: 648 1611 1320 359 159 2042
Fragments arranged by size:
2042 1611 1320 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3555 4232 5380 5625 1606
Size: 1949 677 1148 245 2120
Fragments arranged by size:
2120 1949 1148 677 245
NaeI GCC'GGC
Cuts at: 2723 2892 2723
Size: 169 5970
NcoI C'CATG_G
Cuts at: 513 1065 3144 513
Size: 552 2079 3508
Fragments arranged by size:
3508 2079 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 2721 2890 2721
Size: 169 5970
NheI G'CTAG_C
Cuts at: 1052 3379 1052
Size: 2327 3812
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 3497 5042 1132
Size: 97 2268 1545 2229
Fragments arranged by size:
2268 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6139
PfoI T'CCnGG_A
Cuts at: 2355 2550 3288 2355
Size: 195 738 5206
Fragments arranged by size:
5206 738 195
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 3173 2353
Size: 820 5319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PstI C_TGCA'G
Cuts at: 830 3012 3161 3264 3491 830
Size: 2182 149 103 227 3478
Fragments arranged by size:
3478 2182 227 149 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SanDI GG'GwC_CC
Cuts at: 2353 2353
Size: 6139
SapI GCTCTTCn'nnn_
Cuts at: 3185 3185
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 2270 4387 6099 2270
Size: 2117 1712 2310
Fragments arranged by size:
2310 2117 1712
SexAI A'CCwGG_T
Cuts at: 3039 3039
Size: 6139
SfcI C'TryA_G
Cuts at: 826 1045 2137 2473 3008 3157 3260 3394 3487
Size: 219 1092 336 535 149 103 134 93
Cuts at: 3487 4622 5303 5494 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1092 681 535 336 219 191
149 134 103 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 2721 1700
Size: 1021 5118
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 5144 5406 820
Size: 197 926 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 3236 1517
Size: 1719 4420
StyI C'CwwG_G
Cuts at: 513 1065 3144 3578 513
Size: 552 2079 434 3074
Fragments arranged by size:
3074 2079 552 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2848 4166 4183 4336 4521 4966 325
Size: 1255 1268 1318 17 153 185 445 1498
Fragments arranged by size:
1498 1318 1268 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 4385 6097
Size: 274 80 33 51 1443 291 2117 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
2117 1712 1443 291 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2218 2218
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2197 3947 276
Size: 53 83 186 936 663 1750 2468
Fragments arranged by size:
2468 1750 936 663 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI
SwaI