Restriction map for
pFN21AB0463
(Circular) (Six-base) MAPSORT of: pFN21AB0463.seq Check: 6756 from: 1 to: 6232
pFN21AB0463 6232 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2514 2514
Size: 6232
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 2804 3220 4295 5679 5947 1632
Size: 1124 48 416 1075 1384 268 1917
Fragments arranged by size:
1917 1384 1124 1075 416 268 48
AflII C'TTAA_G
Cuts at: 820 1017 3400 820
Size: 197 2383 3652
Fragments arranged by size:
3652 2383 197
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 2572 2839 2866 3975 5547 2572
Size: 267 27 1109 1572 3257
Fragments arranged by size:
3257 1572 1109 267 27
ApaI G_GGCC'C
Cuts at: 2251 3345 2251
Size: 1094 5138
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 3541 3767 3919 6028 1493
Size: 2048 226 152 2109 1697
Fragments arranged by size:
2109 2048 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 2711 3417 3557 1870
Size: 73 732 36 706 140 4545
Fragments arranged by size:
4545 732 706 140 73 36
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2441 2470 2513 3048
Size: 325 217 627 441 213 29 43 535
Cuts at: 3048 3553 5008 618
Size: 505 1455 1842
Fragments arranged by size:
1842 1455 627 535 505 441 325 217
213 43 29
BanII G_rGCy'C
Cuts at: 1391 2251 2684 3234 3345 3551 1391
Size: 860 433 550 111 206 4072
Fragments arranged by size:
4072 860 550 433 206 111
BbeI G_GCGC'C
Cuts at: 2445 2517 3052 2445
Size: 72 535 5625
Fragments arranged by size:
5625 535 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3001 3343 928
Size: 971 1102 342 3817
Fragments arranged by size:
3817 1102 971 342
BbvCI CC'TCA_GC
Cuts at: 2052 2052
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2542 2576 2702 2736 4423
Size: 34 50 34 521 34 126 34 1687
Cuts at: 4423 4457 1903
Size: 34 3678
Fragments arranged by size:
3678 1687 521 126 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 2868 1548
Size: 1320 4912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2382 2748 3078
Size: 107 122 71 631 605 710 366 330
Cuts at: 3078 4843 136
Size: 1765 1525
Fragments arranged by size:
1765 1525 710 631 605 366 330 122
107 71
BglII A'GATC_T
Cuts at: 3438 6227 3438
Size: 2789 3443
BlpI GC'TnA_GC
Cuts at: 2778 2891 3649 2778
Size: 113 758 5361
Fragments arranged by size:
5361 758 113
Bme1580I G_kGCm'C
Cuts at: 1790 2251 2475 2597 3345 4293 5449 1790
Size: 461 224 122 748 948 1156 2573
Fragments arranged by size:
2573 1156 948 748 461 224 122
BmgBI CAC'GTC
Cuts at: 1318 2181 1318
Size: 863 5369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2076 3963 4921
Size: 416 53 83 399 24 659 1887 958
Cuts at: 4921 442
Size: 1753
Fragments arranged by size:
1887 1753 958 659 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 2787 2816 2825 3146 4892 1239
Size: 969 579 29 9 321 1746 2579
Fragments arranged by size:
2579 1746 969 579 321 29 9
Bpu10I CC'TnA_GC
Cuts at: 2052 2052
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3524 4357 5222 5520 3524
Size: 833 865 298 4236
Fragments arranged by size:
4236 865 833 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3025 4895 882
Size: 617 1526 1870 2219
Fragments arranged by size:
2219 1870 1526 617
BsaAI yAC'GTr
Cuts at: 493 1204 2482 4023 493
Size: 711 1278 1541 2702
Fragments arranged by size:
2702 1541 1278 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 2514 3049 4039
Size: 53 83 186 936 909 72 535 990
Cuts at: 4039 4421 5056 275
Size: 382 635 1451
Fragments arranged by size:
1451 990 936 909 635 535 382 186
83 72 53
BsaWI w'CCGG_w
Cuts at: 3456 4665 5063 5337 5484 5895 3456
Size: 1209 398 274 147 411 3793
Fragments arranged by size:
3793 1209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2628 2658 2979 3009 1178
Size: 30 165 30 1225 30 321 30 4401
Fragments arranged by size:
4401 1225 321 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6232
BseYI C'CCAG_C
Cuts at: 2185 2210 2365 2572 2751 3376 3491 5435 5931
Size: 25 155 207 179 625 115 1944 496
Cuts at: 5931 2185
Size: 2486
Fragments arranged by size:
2486 1944 625 496 207 179 155 115
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5659
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2880 3682 4443 5471 1540
Size: 275 186 879 802 761 1028 2301
Fragments arranged by size:
2301 1028 879 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3234 4293 4378 5449 3234
Size: 1059 85 1071 4017
Fragments arranged by size:
4017 1071 1059 85
BsmI GAATG_Cn'
Cuts at: 2553 3743 3836 2553
Size: 1190 93 4949
Fragments arranged by size:
4949 1190 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2171 3157 2171
Size: 986 5246
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2251 2475 2597 2684 3234 3345 3551
Size: 399 461 224 122 87 550 111 206
Cuts at: 3551 4293 4378 5449 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 461 399 224 206
122 111 87 85
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 4121 5851 4121
Size: 1730 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2514 2647 3587 844
Size: 1670 133 940 3489
Fragments arranged by size:
3489 1670 940 133
BsrBI CCG'CTC
Cuts at: 1394 1823 2815 3040 4119 1394
Size: 429 992 225 1079 3507
Fragments arranged by size:
3507 1079 992 429 225
BsrDI GCAATG_nn'
Cuts at: 65 1248 2871 4727 4901 65
Size: 1183 1623 1856 174 1396
Fragments arranged by size:
1856 1623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2856 4876 5063 1700
Size: 110 1046 2020 187 2869
Fragments arranged by size:
2869 2020 1046 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6232
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 3268 2482
Size: 786 5446
BstYI r'GATC_y
Cuts at: 1972 3438 3562 3929 4314 4331 5783 5869 5881
Size: 1466 124 367 385 17 1452 86 12
Cuts at: 5881 6227 1972
Size: 346 1977
Fragments arranged by size:
1977 1466 1452 385 367 346 124 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2202 2202
Size: 6232
BtgI C'CryG_G
Cuts at: 513 1065 2256 2271 3261 513
Size: 552 1191 15 990 3484
Fragments arranged by size:
3484 1191 990 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 2595 2612 3748 4542 4562 2595
Size: 17 1136 794 20 4265
Fragments arranged by size:
4265 1136 794 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2388 2538
Size: 54 1425 50 275 18 429 129 150
Cuts at: 2538 2609 2816 3162 3225 3679 4568 6197 8
Size: 71 207 346 63 454 889 1629 43
Fragments arranged by size:
1629 1425 889 454 429 346 275 207
150 129 71 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3239 4162 1353
Size: 1886 923 3423
Fragments arranged by size:
3423 1886 923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3232 3232
Size: 6232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2756 2787 2804 2816 2825 3146
Size: 393 576 548 31 17 12 9 321
Cuts at: 3146 3220 4295 4892 5679 5947 1239
Size: 74 1075 597 787 268 1524
Fragments arranged by size:
1524 1075 787 597 576 548 393 321
268 74 31 17 12 9
EcoO109I rG'GnC_Cy
Cuts at: 2070 2198 2207 2248 2717 3245 3341 3342 2070
Size: 128 9 41 469 528 96 1 4960
Fragments arranged by size:
4960 528 469 128 96 41 9 1
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2445 2517 2743 3052 5379 1678
Size: 767 72 226 309 2327 2531
Fragments arranged by size:
2531 2327 767 309 226 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2418 2450 3046 3078 3097
Size: 32 135 32 846 32 596 32 19
Cuts at: 3097 3129 3425 3451 3457 3483 4873 4905 4947
Size: 32 296 26 6 26 1390 32 42
Cuts at: 4947 4979 1373
Size: 32 2626
Fragments arranged by size:
2626 1390 846 596 296 135 42 32
32 32 32 32 32 32 26 26
19 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 3001 3576 3822 669
Size: 988 281 409 654 575 246 3079
Fragments arranged by size:
3079 988 654 575 409 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KasI G'GCGC_C
Cuts at: 2441 2513 3048 2441
Size: 72 535 5625
Fragments arranged by size:
5625 535 72
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 3306 5346 5530 1305
Size: 739 1262 2040 184 2007
Fragments arranged by size:
2040 2007 1262 739 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2390 2540 2611 3164
Size: 54 1425 343 429 129 150 71 553
Cuts at: 3164 10
Size: 3078
Fragments arranged by size:
3078 1425 553 429 343 150 129 71
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3101 3266 3308 4190 4549 4708
Size: 648 1314 621 165 42 882 359 159
Cuts at: 4708 518
Size: 2042
Fragments arranged by size:
2042 1314 882 648 621 359 165 159
42
MspA1I CmG'CkG
Cuts at: 1606 2653 2890 3446 3648 4325 5473 5718 1606
Size: 1047 237 556 202 677 1148 245 2120
Fragments arranged by size:
2120 1148 1047 677 556 245 237 202
NarI GG'CG_CC
Cuts at: 2442 2514 3049 2442
Size: 72 535 5625
Fragments arranged by size:
5625 535 72
NcoI C'CATG_G
Cuts at: 513 1065 3261 513
Size: 552 2196 3484
Fragments arranged by size:
3484 2196 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3085 3590 5135 1132
Size: 97 1856 505 1545 2229
Fragments arranged by size:
2229 1856 1545 505 97
PasI CC'CwG_GG
Cuts at: 1348 2518 1348
Size: 1170 5062
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2244 2572 2607 2839 3067 3213 1108
Size: 1136 328 35 232 228 146 4127
Fragments arranged by size:
4127 1136 328 232 228 146 35
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2070 2207 3245 2070
Size: 137 1038 5057
Fragments arranged by size:
5057 1038 137
PshAI GACnn'nnGTC
Cuts at: 1613 2075 1613
Size: 462 5770
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 2247 3341 2247
Size: 1094 5138
PspXI vC'TCGA_Gb
Cuts at: 3417 3417
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2115 2652 2889 3584 830
Size: 1285 537 237 695 3478
Fragments arranged by size:
3478 1285 695 537 237
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2653 2890 3446 2653
Size: 237 556 5439
Fragments arranged by size:
5439 556 237
SacI G_AGCT'C
Cuts at: 3234 3234
Size: 6232
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SanDI GG'GwC_CC
Cuts at: 2070 2207 3245 2070
Size: 137 1038 5057
Fragments arranged by size:
5057 1038 137
SapI GCTCTTCn'nnn_
Cuts at: 3239 3239
Size: 6232
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SfcI C'TryA_G
Cuts at: 826 1045 2111 2648 2885 3580 4715 5396 5587
Size: 219 1066 537 237 695 1135 681 191
Cuts at: 5587 826
Size: 1471
Fragments arranged by size:
1471 1135 1066 695 681 537 237 219
191
SfoI GGC'GCC
Cuts at: 2443 2515 3050 2443
Size: 72 535 5625
Fragments arranged by size:
5625 535 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2711 3400 3417 3503 4336 5237
Size: 197 926 768 689 17 86 833 901
Cuts at: 5237 5499 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 833 768 689 262 197
86 17
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2422 3015 3212 3261 3671 513
Size: 552 1357 593 197 49 410 3074
Fragments arranged by size:
3074 1357 593 552 410 197 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4478 6190 96
Size: 274 80 33 51 1443 2501 1712 138
Fragments arranged by size:
2501 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2979 3011 6192 6224 2979
Size: 32 3181 32 2987
Fragments arranged by size:
3181 2987 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2903 2269
Size: 634 5598
XhoI C'TCGA_G
Cuts at: 1943 2711 3417 1943
Size: 768 706 4758
Fragments arranged by size:
4758 768 706
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII
SacII SexAI SfiI SrfI SwaI