Restriction map for
pFN21AB0460
(Circular) (Six-base) MAPSORT of: pFN21AB0460.seq Check: 917 from: 1 to: 7603
pFN21AB0460 7603 bp
With 182 enzymes: SgfI *
March 28, 2012 12:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5413 278
Size: 53 83 186 936 3877 2468
Fragments arranged by size:
3877 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2775 3343 4946 1937
Size: 838 568 1603 4594
Fragments arranged by size:
4594 1603 838 568
Acc65I G'GTAC_C
Cuts at: 4924 4924
Size: 7603
AclI AA'CG_TT
Cuts at: 1563 5730 6103 1563
Size: 4167 373 3063
Fragments arranged by size:
4167 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 2243 2364 3063 4187 4824 5666 7050
Size: 409 202 121 699 1124 637 842 1384
Cuts at: 7050 7318 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1124 842 699 637 409 268
202 121
AfeI AGC'GCT
Cuts at: 4404 4404
Size: 7603
AflII C'TTAA_G
Cuts at: 820 1017 2416 820
Size: 197 1399 6007
Fragments arranged by size:
6007 1399 197
AflIII A'CryG_T
Cuts at: 1203 6502 7479 1203
Size: 5299 977 1327
Fragments arranged by size:
5299 1327 977
AgeI A'CCGG_T
Cuts at: 3348 6434 3348
Size: 3086 4517
AhdI GACnn_n'nnGTC
Cuts at: 4669 6332 4669
Size: 1663 5940
AleI CACnn'nnGTG
Cuts at: 1166 2204 3686 1166
Size: 1038 1482 5083
Fragments arranged by size:
5083 1482 1038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7603
AlwNI CAG_nnn'CTG
Cuts at: 2524 4353 4527 5346 6918 2524
Size: 1829 174 819 1572 3209
Fragments arranged by size:
3209 1829 1572 819 174
ApaLI G'TGCA_C
Cuts at: 2183 5660 6816 2183
Size: 3477 1156 2970
Fragments arranged by size:
3477 2970 1156
ApoI r'AATT_y
Cuts at: 1493 2886 3844 4343 4487 4558 4912 5138 5290
Size: 1393 958 499 144 71 354 226 152
Cuts at: 5290 7399 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1393 958 499 354 226 152
144 71
AseI AT'TA_AT
Cuts at: 160 1032 6157 160
Size: 872 5125 1606
Fragments arranged by size:
5125 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7603
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 4084 4928 1870
Size: 73 189 1952 844 4545
Fragments arranged by size:
4545 1952 844 189 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2911 2944 2911
Size: 33 7570
BamHI G'GATC_C
Cuts at: 3672 4933 5300 3672
Size: 1261 367 5975
Fragments arranged by size:
5975 1261 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4376 4391 4924 6379
Size: 325 217 627 1178 1411 15 533 1455
Cuts at: 6379 618
Size: 1842
Fragments arranged by size:
1842 1455 1411 1178 627 533 325 217
15
BanII G_rGCy'C
Cuts at: 1391 2097 2539 3390 3504 4049 4091 4268 4722
Size: 706 442 851 114 545 42 177 454
Cuts at: 4722 4922 1391
Size: 200 4072
Fragments arranged by size:
4072 851 706 545 454 442 200 177
114 42
BbeI G_GCGC'C
Cuts at: 2969 2969
Size: 7603
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 2542 4504 4644 4760 928
Size: 971 453 190 1962 140 116 3771
Fragments arranged by size:
3771 1962 971 453 190 140 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5794 5828 1903
Size: 34 3857 34 3678
Fragments arranged by size:
3857 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3546 5495 6705 3546
Size: 1949 1210 4444
Fragments arranged by size:
4444 1949 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7603
BfrBI ATG'CAT
Cuts at: 3220 3220
Size: 7603
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6214 136
Size: 107 122 71 631 605 4542 1525
Fragments arranged by size:
4542 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2505 7598 2505
Size: 5093 2510
BlpI GC'TnA_GC
Cuts at: 4248 5020 4248
Size: 772 6831
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2716 5664 6820 1790
Size: 397 529 2948 1156 2573
Fragments arranged by size:
2948 2573 1156 529 397
BmgBI CAC'GTC
Cuts at: 1318 3171 1318
Size: 1853 5750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5334 6292 442
Size: 416 53 83 399 24 3917 958 1753
Fragments arranged by size:
3917 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7603
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 3201 3404 4579 2524
Size: 677 203 1175 5548
Fragments arranged by size:
5548 1175 677 203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4147 4383 6263 1239
Size: 2908 236 1880 2579
Fragments arranged by size:
2908 2579 1880 236
Bpu10I CC'TnA_GC
Cuts at: 2027 2264 3389 2027
Size: 237 1125 6241
Fragments arranged by size:
6241 1125 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3452 5728 6593 6891 3452
Size: 2276 865 298 4164
Fragments arranged by size:
4164 2276 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6266 882
Size: 617 4767 2219
Fragments arranged by size:
4767 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5394 493
Size: 711 4190 2702
Fragments arranged by size:
4190 2702 711
BsaBI GATnn'nnATC
Cuts at: 7597 7597
Size: 7603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2966 4679 5410 5792
Size: 53 83 186 936 1433 1713 731 382
Cuts at: 5792 6427 275
Size: 635 1451
Fragments arranged by size:
1713 1451 1433 936 731 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3348 3713 6036 6434 6708 6855 7266 3348
Size: 365 2323 398 274 147 411 3685
Fragments arranged by size:
3685 2323 411 398 365 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4085 4115 1178
Size: 30 165 30 2682 30 4666
Fragments arranged by size:
4666 2682 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2516 4276 4438 1186
Size: 840 490 1760 162 4351
Fragments arranged by size:
4351 1760 840 490 162
BseYI C'CCAG_C
Cuts at: 2524 4027 6806 7302 2524
Size: 1503 2779 496 2825
Fragments arranged by size:
2825 2779 1503 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3851 4228 1163
Size: 573 2115 377 4538
Fragments arranged by size:
4538 2115 573 377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2955 5053 5814 6842 1540
Size: 275 186 954 2098 761 1028 2301
Fragments arranged by size:
2301 2098 1028 954 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2097 2187 2333 3390 3504 5664 5749 6820
Size: 72 90 146 1057 114 2160 85 1071
Cuts at: 6820 2025
Size: 2808
Fragments arranged by size:
2808 2160 1071 1057 146 114 90 85
72
BsmI GAATG_Cn'
Cuts at: 2657 5114 5207 2657
Size: 2457 93 5053
Fragments arranged by size:
5053 2457 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2279 2603 2279
Size: 324 7279
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2097 2187 2333 2539 2716 3390
Size: 399 235 72 90 146 206 177 674
Cuts at: 3390 3504 4049 4091 4268 4722 4922 5664 5749
Size: 114 545 42 177 454 200 742 85
Cuts at: 5749 6820 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 674 545 454 399 235
206 200 177 177 146 114 90 85
72 42
BspEI T'CCGG_A
Cuts at: 3713 7266 3713
Size: 3553 4050
BspHI T'CATG_A
Cuts at: 3600 5492 7222 3600
Size: 1892 1730 3981
Fragments arranged by size:
3981 1892 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 3873 4007 4674 4958 844
Size: 1514 1515 134 667 284 3489
Fragments arranged by size:
3489 1515 1514 667 284 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2546 3424 3436 5490 1394
Size: 429 723 878 12 2054 3507
Fragments arranged by size:
3507 2054 878 723 429 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 3228 6098 6272 65
Size: 1183 1980 2870 174 1396
Fragments arranged by size:
2870 1980 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3348 6247 6434 1700
Size: 110 1538 2899 187 2869
Fragments arranged by size:
2899 2869 1538 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7603
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7603
BssSI C'ACGA_G
Cuts at: 5663 6675 5663
Size: 1012 6591
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7603
BstBI TT'CG_AA
Cuts at: 3370 6407 3370
Size: 3037 4566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7603
BstXI CCAn_nnnn'nTGG
Cuts at: 3589 3688 4111 3589
Size: 99 423 7081
Fragments arranged by size:
7081 423 99
BstYI r'GATC_y
Cuts at: 1972 2505 3054 3672 4933 5300 5685 5702 7154
Size: 533 549 618 1261 367 385 17 1452
Cuts at: 7154 7240 7252 7598 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1261 618 549 533 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3344 3344
Size: 7603
Bsu36I CC'TnA_GG
Cuts at: 4013 4013
Size: 7603
BtgI C'CryG_G
Cuts at: 513 1065 2004 2716 3588 4110 513
Size: 552 939 712 872 522 4006
Fragments arranged by size:
4006 939 872 712 552 522
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2638 4496 487
Size: 683 56 1412 1858 3594
Fragments arranged by size:
3594 1858 1412 683 56
BtsI GCAGTG_nn'
Cuts at: 2383 2389 2525 3528 3696 5119 5913 5933 2383
Size: 6 136 1003 168 1423 794 20 4053
Fragments arranged by size:
4053 1423 1003 794 168 136 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7603
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2773 2808 529
Size: 35 2209 35 5324
Fragments arranged by size:
5324 2209 35 35
DraI TTT'AAA
Cuts at: 4907 4907
Size: 7603
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2293 4762 6610 809
Size: 507 977 2469 1848 1802
Fragments arranged by size:
2469 1848 1802 977 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2580 3647
Size: 54 1425 50 275 18 177 573 1067
Cuts at: 3647 5050 5939 7568 8
Size: 1403 889 1629 43
Fragments arranged by size:
1629 1425 1403 1067 889 573 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5050 1537
Size: 275 3238 4090
Fragments arranged by size:
4090 3238 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2044 2568 3378 3900 4427 4449 4575 4843
Size: 691 524 810 522 527 22 126 268
Cuts at: 4843 5533 1353
Size: 690 3423
Fragments arranged by size:
3423 810 691 690 527 524 522 268
126 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2585 6190 6564 6710 387
Size: 1064 138 996 3605 374 146 1280
Fragments arranged by size:
3605 1280 1064 996 374 146 138
EcoICRI GAG'CTC
Cuts at: 2095 3388 3502 2095
Size: 1293 114 6196
Fragments arranged by size:
6196 1293 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2243 2364 3063 4147 4187 4383
Size: 393 409 202 121 699 1084 40 196
Cuts at: 4383 4824 5666 6263 7050 7318 1239
Size: 441 842 597 787 268 1524
Fragments arranged by size:
1524 1084 842 787 699 597 441 409
393 268 202 196 121 40
EcoO109I rG'GnC_Cy
Cuts at: 2680 3543 4227 4506 2680
Size: 863 684 279 5777
Fragments arranged by size:
5777 863 684 279
EcoRI G'AATT_C
Cuts at: 4343 4912 5290 4343
Size: 569 378 6656
Fragments arranged by size:
6656 569 378
EcoRV GAT'ATC
Cuts at: 2617 3755 2617
Size: 1138 6465
FspI TGC'GCA
Cuts at: 6109 6109
Size: 7603
HaeII r_GCGC'y
Cuts at: 1678 2969 4406 6750 1678
Size: 1291 1437 2344 2531
Fragments arranged by size:
2531 2344 1437 1291
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2746 2778 2831 2863 2999
Size: 32 135 32 1174 32 53 32 136
Cuts at: 2999 3031 3138 3170 3218 3250 3739 3771 3946
Size: 32 107 32 48 32 489 32 175
Cuts at: 3946 3978 4085 4117 4226 4258 4433 4465 4495
Size: 32 107 32 109 32 175 32 30
Cuts at: 4495 4527 4655 4687 4688 4706 4720 4738 4820
Size: 32 128 32 1 18 14 18 82
Cuts at: 4820 4852 6244 6276 6318 6350 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 1174 489 175 175 136 135
128 109 107 107 82 53 48 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 30 18 18 14 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2211 4947 5193 669
Size: 988 281 273 2736 246 3079
Fragments arranged by size:
3079 2736 988 281 273 246
HindIII A'AGCT_T
Cuts at: 748 3428 748
Size: 2680 4923
HpaI GTT'AAC
Cuts at: 5193 5193
Size: 7603
KasI G'GCGC_C
Cuts at: 2965 2965
Size: 7603
KpnI G_GTAC'C
Cuts at: 4928 4928
Size: 7603
MfeI C'AATT_G
Cuts at: 5202 5202
Size: 7603
MluI A'CGCG_T
Cuts at: 7479 7479
Size: 7603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3079 6717 6901 1305
Size: 1774 3638 184 2007
Fragments arranged by size:
3638 2007 1774 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2582 10
Size: 54 1425 343 177 573 5031
Fragments arranged by size:
5031 1425 573 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2204 2874 3582 3686 4109 4713
Size: 648 936 102 670 708 104 423 604
Cuts at: 4713 5561 5920 6079 518
Size: 848 359 159 2042
Fragments arranged by size:
2042 936 848 708 670 648 604 423
359 159 104 102
MspA1I CmG'CkG
Cuts at: 1606 4075 4350 4527 5019 5696 6844 7089 1606
Size: 2469 275 177 492 677 1148 245 2120
Fragments arranged by size:
2469 2120 1148 677 492 275 245 177
NarI GG'CG_CC
Cuts at: 2966 2966
Size: 7603
NcoI C'CATG_G
Cuts at: 513 1065 2004 2716 3588 4110 513
Size: 552 939 712 872 522 4006
Fragments arranged by size:
4006 939 872 712 552 522
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7603
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7603
NotI GC'GGCC_GC
Cuts at: 5050 5050
Size: 7603
NsiI A_TGCA'T
Cuts at: 3222 3222
Size: 7603
NspI r_CATG'y
Cuts at: 1132 1229 3398 4961 6506 1132
Size: 97 2169 1563 1545 2229
Fragments arranged by size:
2229 2169 1563 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4164 1348
Size: 2816 4787
PciI A'CATG_T
Cuts at: 6502 6502
Size: 7603
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3090 1108
Size: 1982 5621
PmeI GTTT'AAAC
Cuts at: 4907 4907
Size: 7603
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5672 5704 7185 7217 5672
Size: 32 1481 32 6058
Fragments arranged by size:
6058 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3543 4227 3543
Size: 684 6919
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7603
PsiI TTA'TAA
Cuts at: 5173 5173
Size: 7603
PspXI vC'TCGA_Gb
Cuts at: 2132 4084 2132
Size: 1952 5651
PstI C_TGCA'G
Cuts at: 830 2520 3241 3480 3918 4120 4431 4955 830
Size: 1690 721 239 438 202 311 524 3478
Fragments arranged by size:
3478 1690 721 524 438 311 239 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7603
PvuII CAG'CTG
Cuts at: 4075 4527 4075
Size: 452 7151
SacI G_AGCT'C
Cuts at: 2097 3390 3504 2097
Size: 1293 114 6196
Fragments arranged by size:
6196 1293 114
SalI G'TCGA_C
Cuts at: 1936 4945 1936
Size: 3009 4594
SapI GCTCTTCn'nnn_
Cuts at: 3378 3378
Size: 7603
SbfI CC_TGCA'GG
Cuts at: 4955 4955
Size: 7603
ScaI AGT'ACT
Cuts at: 5851 7563 5851
Size: 1712 5891
SfcI C'TryA_G
Cuts at: 826 1045 2516 3237 3476 3914 4116 4354 4427
Size: 219 1471 721 239 438 202 238 73
Cuts at: 4427 4885 4951 6086 6767 6958 826
Size: 458 66 1135 681 191 1471
Fragments arranged by size:
1471 1471 1135 721 681 458 438 239
238 219 202 191 73 66
SfoI GGC'GCC
Cuts at: 2967 2967
Size: 7603
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7603
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7603
SmaI CCC'GGG
Cuts at: 1872 4930 1872
Size: 3058 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2416 3431 4084 5707 6608
Size: 197 926 189 284 1015 653 1623 901
Cuts at: 6608 6870 820
Size: 262 1553
Fragments arranged by size:
1623 1553 1015 926 901 653 284 262
197 189
SnaBI TAC'GTA
Cuts at: 493 5394 493
Size: 4901 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7603
SphI G_CATG'C
Cuts at: 1132 3398 4961 1132
Size: 2266 1563 3774
Fragments arranged by size:
3774 2266 1563
SspI AAT'ATT
Cuts at: 5 52 5527 5
Size: 47 5475 2081
Fragments arranged by size:
5475 2081 47
StuI AGG'CCT
Cuts at: 1517 2422 4423 1517
Size: 905 2001 4697
Fragments arranged by size:
4697 2001 905
StyI C'CwwG_G
Cuts at: 513 1065 2004 2539 2716 3588 4110 5042 513
Size: 552 939 535 177 872 522 932 3074
Fragments arranged by size:
3074 939 932 872 552 535 522 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5630 5647 5800 5985 6430 325
Size: 1255 4050 17 153 185 445 1498
Fragments arranged by size:
4050 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2672 3244 5849
Size: 274 80 33 51 1443 695 572 2605
Cuts at: 5849 7561 96
Size: 1712 138
Fragments arranged by size:
2605 1712 1443 695 572 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7563 7595 7563
Size: 32 7571
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7603
XbaI T'CTAG_A
Cuts at: 4939 4939
Size: 7603
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3685 3685
Size: 7603
XhoI C'TCGA_G
Cuts at: 1943 2132 4084 1943
Size: 189 1952 5462
Fragments arranged by size:
5462 1952 189
XmaI C'CCGG_G
Cuts at: 1870 4928 1870
Size: 3058 4545
XmnI GAAnn'nnTTC
Cuts at: 3366 5732 3366
Size: 2366 5237
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5411 276
Size: 53 83 186 936 3877 2468
Fragments arranged by size:
3877 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BsiWI DraIII
EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI
PfoI PmlI PspOMI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI