Restriction map for
pFN21AB0455
(Circular) (Six-base) MAPSORT of: pFN21AB0455.seq Check: 8447 from: 1 to: 6220
pFN21AB0455 6220 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3389 3389
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2264 2288 2837 2996 3060 4283 5667
Size: 470 162 24 549 159 64 1223 1384
Cuts at: 5667 5935 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1223 549 470 268 162 159
64 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 3021 3255 5119 6096 1203
Size: 1818 234 1864 977 1327
Fragments arranged by size:
1864 1818 1327 977 234
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 3259 4949 3259
Size: 1690 4530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2436 1184
Size: 1252 4968
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2360 2392 2818 2850 2360
Size: 32 426 32 5730
Fragments arranged by size:
5730 426 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2242 2266 2605 2650 3963 5535 2242
Size: 24 339 45 1313 1572 2927
Fragments arranged by size:
2927 1572 1313 339 45 24
ApaI G_GGCC'C
Cuts at: 2695 2695
Size: 6220
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 3067 3529 3755 3907 6016 1493
Size: 1574 462 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 462 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2346 2744 3329 3389 3434 3545 1870
Size: 73 403 398 585 60 45 111 4545
Fragments arranged by size:
4545 585 403 398 111 73 60 45
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2477 3304 3541 4996 618
Size: 325 217 627 690 827 237 1455 1842
Fragments arranged by size:
1842 1455 827 690 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2078 2695 2925 3539 1391
Size: 687 617 230 614 4072
Fragments arranged by size:
4072 687 617 614 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2125 2184 2984 3229 928
Size: 971 226 59 800 245 3919
Fragments arranged by size:
3919 971 800 245 226 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 2523 1548
Size: 975 5245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2695 4281 5437 1790
Size: 541 364 1586 1156 2573
Fragments arranged by size:
2573 1586 1156 541 364
BmgBI CAC'GTC
Cuts at: 1318 3256 1318
Size: 1938 4282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 3951 4909
Size: 416 53 83 399 24 908 1626 958
Cuts at: 4909 442
Size: 1753
Fragments arranged by size:
1753 1626 958 908 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 2582 2820 3368 3494 4880 1239
Size: 1059 284 238 548 126 1386 2579
Fragments arranged by size:
2579 1386 1059 548 284 238 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4345 5210 5508 4345
Size: 865 298 5057
Fragments arranged by size:
5057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 3405 4027 4409
Size: 53 83 186 936 573 1299 622 382
Cuts at: 4409 5044 275
Size: 635 1451
Fragments arranged by size:
1451 1299 936 635 622 573 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 2818 2848 1178
Size: 30 165 30 1152 30 233 30 4550
Fragments arranged by size:
4550 1152 233 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2139 3452 1186
Size: 953 1313 3954
Fragments arranged by size:
3954 1313 953
BseYI C'CCAG_C
Cuts at: 5423 5919 5423
Size: 496 5724
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2630 1163
Size: 573 894 4753
Fragments arranged by size:
4753 894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4281 4366 5437 4281
Size: 85 1071 5064
Fragments arranged by size:
5064 1071 85
BsmI GAATG_Cn'
Cuts at: 3731 3824 3731
Size: 93 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2331 2695 2925 3539 4281 4366
Size: 399 288 253 364 230 614 742 85
Cuts at: 4366 5437 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 614 399 364 288 253
230 85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 3093 3389 3575 844
Size: 1397 852 296 186 3489
Fragments arranged by size:
3489 1397 852 296 186
BsrBI CCG'CTC
Cuts at: 1394 1823 2887 3442 3499 4107 1394
Size: 429 1064 555 57 608 3507
Fragments arranged by size:
3507 1064 608 555 429 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2438 3429 4864 5051 1700
Size: 110 628 991 1435 187 2869
Fragments arranged by size:
2869 1435 991 628 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 2882 4280 5292 2882
Size: 1398 1012 3810
Fragments arranged by size:
3810 1398 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3469 794
Size: 2675 3545
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 2911 1184
Size: 1727 4493
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3103 2011
Size: 1092 5128
BstYI r'GATC_y
Cuts at: 1972 2538 2709 3550 3917 4302 4319 5771 5857
Size: 566 171 841 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 841 566 385 367 346 171
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3400 513
Size: 552 939 1396 3333
Fragments arranged by size:
3333 1396 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3394 487
Size: 683 56 2168 3313
Fragments arranged by size:
3313 2168 683 56
BtsI GCAGTG_nn'
Cuts at: 2114 2913 3736 4530 4550 2114
Size: 799 823 794 20 3784
Fragments arranged by size:
3784 823 799 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 2802 2949
Size: 54 1425 50 275 18 462 510 147
Cuts at: 2949 3093 3489 3667 4556 6185 8
Size: 144 396 178 889 1629 43
Fragments arranged by size:
1629 1425 889 510 462 396 275 178
147 144 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2308 4150 1353
Size: 955 1842 3423
Fragments arranged by size:
3423 1842 955
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2264 2288 2298 2582 2820 2837
Size: 393 470 162 24 10 284 238 17
Cuts at: 2837 2996 3060 3368 3494 4283 4880 5667 5935
Size: 159 64 308 126 789 597 787 268
Cuts at: 5935 1239
Size: 1524
Fragments arranged by size:
1524 789 787 597 470 393 308 284
268 238 162 159 126 64 24 17
10
EcoNI CCTnn'n_nnAGG
Cuts at: 2852 2852
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2230 2263 2602 2230
Size: 33 339 5848
Fragments arranged by size:
5848 339 33
EcoRI G'AATT_C
Cuts at: 3067 3529 3907 3067
Size: 462 378 5380
Fragments arranged by size:
5380 462 378
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2094 5367 1678
Size: 416 3273 2531
Fragments arranged by size:
3273 2531 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2857 2889 2988 3020 3245
Size: 32 135 32 1285 32 99 32 225
Cuts at: 3245 3277 4861 4893 4935 4967 1373
Size: 32 1584 32 42 32 2626
Fragments arranged by size:
2626 1584 1285 225 135 99 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2375 2920 5334 5518 1305
Size: 1070 545 2414 184 2007
Fragments arranged by size:
2414 2007 1070 545 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 2804 2951 10
Size: 54 1425 343 462 510 147 3279
Fragments arranged by size:
3279 1425 510 462 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2216 2798 3104 4178 4537 4696
Size: 648 1006 44 582 306 1074 359 159
Cuts at: 4696 518
Size: 2042
Fragments arranged by size:
2042 1074 1006 648 582 359 306 159
44
MspA1I CmG'CkG
Cuts at: 1606 2544 3402 3636 4313 5461 5706 1606
Size: 938 858 234 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 858 677 245 234
NaeI GCC'GGC
Cuts at: 2440 3431 2440
Size: 991 5229
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4729
Fragments arranged by size:
4729 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NgoMIV G'CCGG_C
Cuts at: 2438 3429 2438
Size: 991 5229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3025 3367 3578 5123 1132
Size: 97 1796 342 211 1545 2229
Fragments arranged by size:
2229 1796 1545 342 211 97
PasI CC'CwG_GG
Cuts at: 1348 2512 2688 3314 1348
Size: 1164 176 626 4254
Fragments arranged by size:
4254 1164 626 176
PciI A'CATG_T
Cuts at: 3021 5119 3021
Size: 2098 4122
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 3204 1108
Size: 1497 599 4124
Fragments arranged by size:
4124 1497 599
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2850 4289 4321 5802 5834 2818
Size: 32 1439 32 1481 32 3204
Fragments arranged by size:
3204 1481 1439 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2263 2230
Size: 33 6187
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2691 2691
Size: 6220
PstI C_TGCA'G
Cuts at: 830 2238 2880 3465 3516 3572 830
Size: 1408 642 585 51 56 3478
Fragments arranged by size:
3478 1408 642 585 56 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
SacII CC_GC'GG
Cuts at: 3403 3403
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2234 2258 2876 3461 3512 3568 4703
Size: 219 1189 24 618 585 51 56 1135
Cuts at: 4703 5384 5575 826
Size: 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 618 585 219 191
56 51 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4324 5225 5487 820
Size: 197 926 2381 901 262 1553
Fragments arranged by size:
2381 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3367 3578 1132
Size: 2235 211 3774
Fragments arranged by size:
3774 2235 211
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 3116 1517
Size: 1599 4621
StyI C'CwwG_G
Cuts at: 513 1065 2004 2916 3008 3659 513
Size: 552 939 912 92 651 3074
Fragments arranged by size:
3074 939 912 651 552 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2525 2840 4247 4264 4417 4602 5047
Size: 1255 945 315 1407 17 153 185 445
Cuts at: 5047 325
Size: 1498
Fragments arranged by size:
1498 1407 1255 945 445 315 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2943 4466 6178
Size: 274 80 33 51 1443 966 1523 1712
Cuts at: 6178 96
Size: 138
Fragments arranged by size:
1712 1523 1443 966 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2338
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 4349 4349
Size: 6220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI