Restriction map for
pFN21AB0453
(Circular) (Six-base) MAPSORT of: pFN21AB0453.seq Check: 7448 from: 1 to: 6220
pFN21AB0453 6220 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 3563 1937
Size: 784 842 4594
Fragments arranged by size:
4594 842 784
Acc65I G'GTAC_C
Cuts at: 3033 3541 3033
Size: 508 5712
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 4283 5667 5935 1632
Size: 965 1686 1384 268 1917
Fragments arranged by size:
1917 1686 1384 965 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2163 5119 6096 1203
Size: 960 2956 977 1327
Fragments arranged by size:
2956 1327 977 960
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 2213 1166
Size: 1047 5173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 3109 3121 3963 5535 3109
Size: 12 842 1572 3794
Fragments arranged by size:
3794 1572 842 12
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2443 2864 3529 3755 3907 6016 1493
Size: 950 421 665 226 152 2109 1697
Fragments arranged by size:
2109 1697 950 665 421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2403 2945 3545 1870
Size: 73 205 255 542 600 4545
Fragments arranged by size:
4545 600 542 255 205 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2950 3033 3541 4996 618
Size: 325 217 627 1163 83 508 1455 1842
Fragments arranged by size:
1842 1455 1163 627 508 325 217 83
BanII G_rGCy'C
Cuts at: 1391 3539 1391
Size: 2148 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5249
BbvCI CC'TCA_GC
Cuts at: 3479 3479
Size: 6220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3145 3179 4411 4445 1903
Size: 34 1208 34 1232 34 3678
Fragments arranged by size:
3678 1232 1208 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2955 3496 4281 5437 1790
Size: 1165 541 785 1156 2573
Fragments arranged by size:
2573 1165 1156 785 541
BmgBI CAC'GTC
Cuts at: 1318 2743 1318
Size: 1425 4795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3267 3267
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 2891 3143 4880 1239
Size: 1292 360 252 1737 2579
Fragments arranged by size:
2579 1737 1292 360 252
Bpu10I CC'TnA_GC
Cuts at: 3479 3479
Size: 6220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3262 3455 4345 5210 5508 3262
Size: 193 890 865 298 3974
Fragments arranged by size:
3974 890 865 298 193
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2164 4011 493
Size: 711 960 1847 2702
Fragments arranged by size:
2702 1847 960 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3436 4027 4409 5044
Size: 53 83 186 936 1903 591 382 635
Cuts at: 5044 275
Size: 1451
Fragments arranged by size:
1903 1451 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5995
Fragments arranged by size:
5995 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 2375 2425 1186
Size: 1120 69 50 4981
Fragments arranged by size:
4981 1120 69 50
BseYI C'CCAG_C
Cuts at: 5423 5919 5423
Size: 496 5724
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3134 1163
Size: 573 1398 4249
Fragments arranged by size:
4249 1398 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2323 4281 4366 5437 2323
Size: 1958 85 1071 3106
Fragments arranged by size:
3106 1958 1071 85
BsmI GAATG_Cn'
Cuts at: 2594 3731 3824 2594
Size: 1137 93 4990
Fragments arranged by size:
4990 1137 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2323 2955 3496 3539 4281 4366 5437
Size: 399 533 632 541 43 742 85 1071
Cuts at: 5437 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 632 541 533 399 85
43
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 2730 4109 5839 2730
Size: 1379 1730 3111
Fragments arranged by size:
3111 1730 1379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3575 844
Size: 2731 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 3163 4715 4889 65
Size: 1183 1245 670 1552 174 1396
Fragments arranged by size:
1552 1396 1245 1183 670 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2769 3462 4864 5051 1700
Size: 110 959 693 1402 187 2869
Fragments arranged by size:
2869 1402 959 693 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 2068 3422 5024 2068
Size: 1354 1602 3264
Fragments arranged by size:
3264 1602 1354
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 2509 2509
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2304 2638 3550 3917 4302 4319 5771 5857
Size: 332 334 912 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 912 385 367 346 334 332
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2969 3736 4530 4550 2969
Size: 767 794 20 4639
Fragments arranged by size:
4639 794 767 20
ClaI AT'CG_AT
Cuts at: 1554 3079 1554
Size: 1525 4695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3667 4556 6185
Size: 54 1425 50 275 18 1837 889 1629
Cuts at: 6185 8
Size: 43
Fragments arranged by size:
1837 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2573 2912 2997 3036 4150 1353
Size: 1220 339 85 39 1114 3423
Fragments arranged by size:
3423 1220 1114 339 85 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2531 2597 2891 3143 4283 4880 5667
Size: 393 899 66 294 252 1140 597 787
Cuts at: 5667 5935 1239
Size: 268 1524
Fragments arranged by size:
1524 1140 899 787 597 393 294 268
252 66
EcoO109I rG'GnC_Cy
Cuts at: 3106 3106
Size: 6220
EcoRI G'AATT_C
Cuts at: 2443 3529 3907 2443
Size: 1086 378 4756
Fragments arranged by size:
4756 1086 378
EcoRV GAT'ATC
Cuts at: 3083 3083
Size: 6220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2333 5367 1678
Size: 655 3034 2531
Fragments arranged by size:
3034 2531 655
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2667 2699 3237 3269 4861
Size: 32 135 32 1095 32 538 32 1592
Cuts at: 4861 4893 4935 4967 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1592 1095 538 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 3564 3810 669
Size: 988 281 748 878 246 3079
Fragments arranged by size:
3079 988 878 748 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3037 3545 3037
Size: 508 5712
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4398
Fragments arranged by size:
4398 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2286 2654 4178 4537 4696 518
Size: 648 1047 73 368 1524 359 159 2042
Fragments arranged by size:
2042 1524 1047 648 368 359 159 73
MspA1I CmG'CkG
Cuts at: 1606 3118 3636 4313 5461 5706 1606
Size: 1512 518 677 1148 245 2120
Fragments arranged by size:
2120 1512 1148 677 518 245
NaeI GCC'GGC
Cuts at: 2771 3464 2771
Size: 693 5527
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NgoMIV G'CCGG_C
Cuts at: 2769 3462 2769
Size: 693 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3505 3578 5123 1132
Size: 97 2276 73 1545 2229
Fragments arranged by size:
2276 2229 1545 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3109 1108
Size: 2001 4219
PfoI T'CCnGG_A
Cuts at: 2694 2694
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3106 3106
Size: 6220
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PstI C_TGCA'G
Cuts at: 830 3572 830
Size: 2742 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SexAI A'CCwGG_T
Cuts at: 3108 3108
Size: 6220
SfcI C'TryA_G
Cuts at: 826 1045 3568 4703 5384 5575 826
Size: 219 2523 1135 681 191 1471
Fragments arranged by size:
2523 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 2150 2405 3547 1872
Size: 278 255 1142 4545
Fragments arranged by size:
4545 1142 278 255
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3241 3470 4324 5225 5487 820
Size: 197 926 1298 229 854 901 262 1553
Fragments arranged by size:
1553 1298 926 901 854 262 229 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 2506 4144 5
Size: 47 2454 1638 2081
Fragments arranged by size:
2454 2081 1638 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 3659 513
Size: 552 2594 3074
Fragments arranged by size:
3074 2594 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3279 4247 4264 4417 4602 5047 325
Size: 1255 1699 968 17 153 185 445 1498
Fragments arranged by size:
1699 1498 1255 968 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 2148 2403 3545 1870
Size: 278 255 1142 4545
Fragments arranged by size:
4545 1142 278 255
XmnI GAAnn'nnTTC
Cuts at: 2447 4349 2447
Size: 1902 4318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI