Restriction map for
pFN21AB0447
(Circular) (Six-base) MAPSORT of: pFN21AB0447.seq Check: 7554 from: 1 to: 6208
pFN21AB0447 6208 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 2683 2770 4271 5655 5923 1632
Size: 833 218 87 1501 1384 268 1917
Fragments arranged by size:
1917 1501 1384 833 268 218 87
AfeI AGC'GCT
Cuts at: 2053 3474 2053
Size: 1421 4787
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 2539 5107 6084 1203
Size: 1336 2568 977 1327
Fragments arranged by size:
2568 1336 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 3323 3371 1166
Size: 2157 48 4003
Fragments arranged by size:
4003 2157 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2384 2560 2864 1184
Size: 1200 176 304 4528
Fragments arranged by size:
4528 1200 304 176
AlwNI CAG_nnn'CTG
Cuts at: 2221 3951 5523 2221
Size: 1730 1572 2906
Fragments arranged by size:
2906 1730 1572
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 2295 2978 3446 3517 3743 3895 6004 1493
Size: 802 683 468 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 802 683 468 226 152 71
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2751 3533 1870
Size: 73 808 782 4545
Fragments arranged by size:
4545 808 782 73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2119 3529 4984 618
Size: 325 217 627 278 54 1410 1455 1842
Fragments arranged by size:
1842 1455 1410 627 325 278 217 54
BanII G_rGCy'C
Cuts at: 1391 2253 2454 2603 2730 2760 2871 3141 3527
Size: 862 201 149 127 30 111 270 386
Cuts at: 3527 1391
Size: 4072
Fragments arranged by size:
4072 862 386 270 201 149 127 111
30
BbeI G_GCGC'C
Cuts at: 2069 2069
Size: 6208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2174 3303 928
Size: 971 275 1129 3833
Fragments arranged by size:
3833 1129 971 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4399 4433 1903
Size: 34 50 34 2378 34 3678
Fragments arranged by size:
3678 2378 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2758 4100 5310 2758
Size: 1342 1210 3656
Fragments arranged by size:
3656 1342 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2568 4819 136
Size: 107 122 71 631 605 896 2251 1525
Fragments arranged by size:
2251 1525 896 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3081 6203 3081
Size: 3122 3086
BlpI GC'TnA_GC
Cuts at: 2372 2922 3123 3625 2372
Size: 550 201 502 4955
Fragments arranged by size:
4955 550 502 201
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2115 4269 5425 1790
Size: 290 35 2154 1156 2573
Fragments arranged by size:
2573 2154 1156 290 35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2067 3271 3939
Size: 416 53 83 399 24 650 1204 668
Cuts at: 3939 4897 442
Size: 958 1753
Fragments arranged by size:
1753 1204 958 668 650 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2131 2237 2669 3137 3257 3415 4868 1239
Size: 892 106 432 468 120 158 1453 2579
Fragments arranged by size:
2579 1453 892 468 432 158 120 106
Bpu10I CC'TnA_GC
Cuts at: 2557 2557
Size: 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2924 4333 5198 5496 2600
Size: 324 1409 865 298 3312
Fragments arranged by size:
3312 1409 865 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2242 2414 2465 3449 4871 882
Size: 617 743 172 51 984 1422 2219
Fragments arranged by size:
2219 1422 984 743 617 172 51
BsaAI yAC'GTr
Cuts at: 493 1204 3999 493
Size: 711 2795 2702
Fragments arranged by size:
2795 2702 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2090 4015 4397
Size: 53 83 186 936 533 24 1925 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1925 1451 936 635 533 382 186 83
53 24
BsaWI w'CCGG_w
Cuts at: 2799 3003 3359 4641 5039 5313 5460 5871 2799
Size: 204 356 1282 398 274 147 411 3136
Fragments arranged by size:
3136 1282 411 398 356 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2053 2083 3071 3098 3101
Size: 30 165 30 650 30 988 27 3
Cuts at: 3101 3128 3212 3242 1178
Size: 27 84 30 4144
Fragments arranged by size:
4144 988 650 165 84 30 30 30
30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2115 2597 3217 3518 1186
Size: 929 482 620 301 3876
Fragments arranged by size:
3876 929 620 482 301
BseYI C'CCAG_C
Cuts at: 5411 5907 5411
Size: 496 5712
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5635
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 3658 4419 5447 1540
Size: 275 186 658 999 761 1028 2301
Fragments arranged by size:
2301 1028 999 761 658 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 2454 2603 2730 3141 4269 4354 5425 2253
Size: 201 149 127 411 1128 85 1071 3036
Fragments arranged by size:
3036 1128 1071 411 201 149 127 85
BsiWI C'GTAC_G
Cuts at: 2862 2862
Size: 6208
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2115 2253 2454 2603 2730 2760
Size: 399 290 35 138 201 149 127 30
Cuts at: 2760 2871 3141 3527 4269 4354 5425 1391
Size: 111 270 386 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 386 290 270 201
149 138 127 111 85 35 30
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1999 2206 2528 2787 3563 844
Size: 1155 207 322 259 776 3489
Fragments arranged by size:
3489 1155 776 322 259 207
BsrBI CCG'CTC
Cuts at: 1394 1823 4095 1394
Size: 429 2272 3507
Fragments arranged by size:
3507 2272 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2473 4703 4877 65
Size: 1183 1225 2230 174 1396
Fragments arranged by size:
2230 1396 1225 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2658 4852 5039 1700
Size: 110 848 2194 187 2869
Fragments arranged by size:
2869 2194 848 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 2105 1925
Size: 180 6028
BssSI C'ACGA_G
Cuts at: 2915 4268 5280 2915
Size: 1353 1012 3843
Fragments arranged by size:
3843 1353 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2119 794
Size: 1325 4883
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 2627 3316 1184
Size: 1443 689 4076
Fragments arranged by size:
4076 1443 689
BstYI r'GATC_y
Cuts at: 1972 3021 3081 3538 3905 4290 4307 5759 5845
Size: 1049 60 457 367 385 17 1452 86
Cuts at: 5845 5857 6203 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1049 457 385 367 346 86
60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2931 3036 3321 3459 513
Size: 552 1866 105 285 138 3262
Fragments arranged by size:
3262 1866 552 285 138 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 2366 3724 4518 4538 2366
Size: 1358 794 20 4036
Fragments arranged by size:
4036 1358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DraIII CAC_nnn'GTG
Cuts at: 2119 2119
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2234 5215 809
Size: 507 918 2981 1802
Fragments arranged by size:
2981 1802 918 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2569 3655 4544
Size: 54 1425 50 275 18 739 1086 889
Cuts at: 4544 6173 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1086 889 739 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 2487 2735 4138 1353
Size: 996 138 248 1403 3423
Fragments arranged by size:
3423 1403 996 248 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3014 3479 4795 5169 5315 387
Size: 1064 138 1425 465 1316 374 146 1280
Fragments arranged by size:
1425 1316 1280 1064 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2452 2601 2728 3139 2251
Size: 201 149 127 411 5320
Fragments arranged by size:
5320 411 201 149 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2131 2237 2465 2669 2683 2770 3137
Size: 393 499 106 228 204 14 87 367
Cuts at: 3137 3257 3415 4271 4868 5655 5923 1239
Size: 120 158 856 597 787 268 1524
Fragments arranged by size:
1524 856 787 597 499 393 367 268
228 204 158 120 106 87 14
EcoO109I rG'GnC_Cy
Cuts at: 2218 2218
Size: 6208
EcoRI G'AATT_C
Cuts at: 2978 3517 3895 2978
Size: 539 378 5291
Fragments arranged by size:
5291 539 378
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2055 2069 3476 5355 1678
Size: 377 14 1407 1879 2531
Fragments arranged by size:
2531 1879 1407 377 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 2317 2349 3071
Size: 32 135 32 583 32 130 32 722
Cuts at: 3071 3103 4849 4881 4923 4955 1373
Size: 32 1746 32 42 32 2626
Fragments arranged by size:
2626 1746 722 583 135 130 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 3171 3552 3798 669
Size: 988 281 373 860 381 246 3079
Fragments arranged by size:
3079 988 860 381 373 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 2065 2065
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 2539 6084 2539
Size: 3545 2663
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4386
Fragments arranged by size:
4386 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3323 3371 3407 4166 4525 4684 518
Size: 648 2157 48 36 759 359 159 2042
Fragments arranged by size:
2157 2042 759 648 359 159 48 36
MspA1I CmG'CkG
Cuts at: 1606 2125 3244 3624 4301 5449 5694 1606
Size: 519 1119 380 677 1148 245 2120
Fragments arranged by size:
2120 1148 1119 677 519 380 245
NarI GG'CG_CC
Cuts at: 2066 2066
Size: 6208
NcoI C'CATG_G
Cuts at: 513 1065 2931 513
Size: 552 1866 3790
Fragments arranged by size:
3790 1866 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2677 3566 5111 1132
Size: 97 1448 889 1545 2229
Fragments arranged by size:
2229 1545 1448 889 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PfoI T'CCnGG_A
Cuts at: 2253 2733 3431 2253
Size: 480 698 5030
Fragments arranged by size:
5030 698 480
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2585 2617 4277 4309 5790 5822 2585
Size: 32 1660 32 1481 32 2971
Fragments arranged by size:
2971 1660 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 2218
Size: 6208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2549 3480 3560 830
Size: 1719 931 80 3478
Fragments arranged by size:
3478 1719 931 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 3244 3244
Size: 6208
SacI G_AGCT'C
Cuts at: 2253 2454 2603 2730 3141 2253
Size: 201 149 127 411 5320
Fragments arranged by size:
5320 411 201 149 127
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SapI GCTCTTCn'nnn_
Cuts at: 2349 2735 2349
Size: 386 5822
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 3305 4456 6168 3305
Size: 1151 1712 3345
Fragments arranged by size:
3345 1712 1151
SfcI C'TryA_G
Cuts at: 826 1045 2545 3420 3476 3556 4691 5372 5563
Size: 219 1500 875 56 80 1135 681 191
Cuts at: 5563 826
Size: 1471
Fragments arranged by size:
1500 1471 1135 875 681 219 191 80
56
SfiI GGCCn_nnn'nGGCC
Cuts at: 2568 2568
Size: 6208
SfoI GGC'GCC
Cuts at: 2067 2067
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2753 3535 1872
Size: 881 782 4545
Fragments arranged by size:
4545 881 782
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 2903 4312 5213 5475 820
Size: 197 926 636 324 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 636 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 2562 2806 1517
Size: 1045 244 4919
Fragments arranged by size:
4919 1045 244
StyI C'CwwG_G
Cuts at: 513 1065 2931 3647 513
Size: 552 1866 716 3074
Fragments arranged by size:
3074 1866 716 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2144 3253 4235 4252 4405 4590 5035
Size: 1255 564 1109 982 17 153 185 445
Cuts at: 5035 325
Size: 1498
Fragments arranged by size:
1498 1255 1109 982 564 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3303 4454 6166
Size: 274 80 33 51 1443 1326 1151 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
1712 1443 1326 1151 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2611 2643 6168 6200 2611
Size: 32 3525 32 2619
Fragments arranged by size:
3525 2619 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2751 3533 1870
Size: 881 782 4545
Fragments arranged by size:
4545 881 782
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SrfI
SwaI XcmI