Restriction map for
pFN21AB0445
(Circular) (Six-base) MAPSORT of: pFN21AB0445.seq Check: 272 from: 1 to: 6178
pFN21AB0445 6178 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2122 2318 2122
Size: 196 5982
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3521 1937
Size: 1584 4594
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 3214 4241 5625 5893 1632
Size: 581 1001 1027 1384 268 1917
Fragments arranged by size:
1917 1384 1027 1001 581 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 2385 2931 5077 6054 1203
Size: 1182 546 2146 977 1327
Fragments arranged by size:
2146 1327 1182 977 546
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 6146
AlwNI CAG_nnn'CTG
Cuts at: 2398 2410 2467 2944 3121 3382 3921 5493 2398
Size: 12 57 477 177 261 539 1572 3083
Fragments arranged by size:
3083 1572 539 477 261 177 57 12
ApaI G_GGCC'C
Cuts at: 2430 2474 2430
Size: 44 6134
ApaLI G'TGCA_C
Cuts at: 3321 4235 5391 3321
Size: 914 1156 4108
Fragments arranged by size:
4108 1156 914
ApoI r'AATT_y
Cuts at: 1493 2657 3487 3713 3865 5974 1493
Size: 1164 830 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 830 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2253 2743 3355 3503 1870
Size: 73 310 490 612 148 4545
Fragments arranged by size:
4545 612 490 310 148 73
BamHI G'GATC_C
Cuts at: 3508 3875 3508
Size: 367 5811
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2791 3499 4954 618
Size: 325 217 627 1004 708 1455 1842
Fragments arranged by size:
1842 1455 1004 708 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 2094 2430 2474 3162 3336 3497 1391
Size: 634 69 336 44 688 174 161 4072
Fragments arranged by size:
4072 688 634 336 174 161 69 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 928
Size: 971 786 4421
Fragments arranged by size:
4421 971 786
BbvCI CC'TCA_GC
Cuts at: 3056 3056
Size: 6178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 2264 3066
Size: 107 122 71 631 605 380 212 802
Cuts at: 3066 3387 4789 136
Size: 321 1402 1525
Fragments arranged by size:
1525 1402 802 631 605 380 321 212
122 107 71
BglII A'GATC_T
Cuts at: 6173 6173
Size: 6178
BlpI GC'TnA_GC
Cuts at: 2033 2447 3161 3595 2033
Size: 414 714 434 4616
Fragments arranged by size:
4616 714 434 414
Bme1580I G_kGCm'C
Cuts at: 1790 2430 2474 2796 3325 3438 4239 5395 1790
Size: 640 44 322 529 113 801 1156 2573
Fragments arranged by size:
2573 1156 801 640 529 322 113 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 3454 3909
Size: 416 53 83 399 24 1895 142 455
Cuts at: 3909 4867 442
Size: 958 1753
Fragments arranged by size:
1895 1753 958 455 416 399 142 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2746 2864 3176 3359 4838 1239
Size: 1507 118 312 183 1479 2579
Fragments arranged by size:
2579 1507 1479 312 183 118
Bpu10I CC'TnA_GC
Cuts at: 2422 3015 3056 2422
Size: 593 41 5544
Fragments arranged by size:
5544 593 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3320 4303 5168 5466 2138
Size: 1182 983 865 298 2850
Fragments arranged by size:
2850 1182 983 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2214 4841 882
Size: 617 715 2627 2219
Fragments arranged by size:
2627 2219 715 617
BsaAI yAC'GTr
Cuts at: 493 1204 2820 3969 493
Size: 711 1616 1149 2702
Fragments arranged by size:
2702 1616 1149 711
BsaBI GATnn'nnATC
Cuts at: 6172 6172
Size: 6178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2133 2150 2163 2270
Size: 30 165 30 717 13 17 13 107
Cuts at: 2270 2300 1178
Size: 30 5056
Fragments arranged by size:
5056 717 165 107 30 30 30 17
13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2687 3461 1186
Size: 955 546 774 3903
Fragments arranged by size:
3903 955 774 546
BseYI C'CCAG_C
Cuts at: 3064 5381 5877 3064
Size: 2317 496 3365
Fragments arranged by size:
3365 2317 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2396 2954 3158 1163
Size: 573 660 558 204 4183
Fragments arranged by size:
4183 660 573 558 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2377 3162 3325 3336 4239 4324 5395 2034
Size: 343 785 163 11 903 85 1071 2817
Fragments arranged by size:
2817 1071 903 785 343 163 85 11
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2034 2094 2377 2430 2474 2796
Size: 399 235 9 60 283 53 44 322
Cuts at: 2796 3162 3325 3336 3438 3497 4239 4324 5395
Size: 366 163 11 102 59 742 85 1071
Cuts at: 5395 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 366 322 283 235
163 102 85 60 59 53 44 11
9
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 4067 5797 4067
Size: 1730 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 2318 2514 3105 3533 844
Size: 1278 196 196 591 428 3489
Fragments arranged by size:
3489 1278 591 428 196 196
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3198 4673 4847 65
Size: 1183 1950 1475 174 1396
Fragments arranged by size:
1950 1475 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 4822 5009 1700
Size: 110 290 2722 187 2869
Fragments arranged by size:
2869 2722 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2372 2398 2407 794
Size: 1578 26 9 4565
Fragments arranged by size:
4565 1578 26 9
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstXI CCAn_nnnn'nTGG
Cuts at: 2174 2174
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2856 3105 3508 3875 4260 4277 5729 5815
Size: 884 249 403 367 385 17 1452 86
Cuts at: 5815 5827 6173 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 884 403 385 367 346 249
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 2637 2796 3326 3397 3441
Size: 552 939 81 552 159 530 71 44
Cuts at: 3441 513
Size: 3250
Fragments arranged by size:
3250 939 552 552 530 159 81 71
44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 3694 4488 4508 2271
Size: 1423 794 20 3941
Fragments arranged by size:
3941 1423 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2902 2937 3181 3216 529
Size: 35 2338 35 244 35 3491
Fragments arranged by size:
3491 2338 244 35 35 35
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DraIII CAC_nnn'GTG
Cuts at: 2372 2372
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2179 2946 3048
Size: 54 1425 50 275 18 349 767 102
Cuts at: 3048 3189 3625 4514 6143 8
Size: 141 436 889 1629 43
Fragments arranged by size:
1629 1425 889 767 436 349 275 141
102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 3257 3293 4108 1353
Size: 836 1068 36 815 3423
Fragments arranged by size:
3423 1068 836 815 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3160 3334 3160
Size: 174 6004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2746 2864 3176 3214 3359 4241
Size: 393 581 533 118 312 38 145 882
Cuts at: 4241 4838 5625 5893 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 882 787 597 581 533 393 312
268 145 118 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2459 2459
Size: 6178
EcoO109I rG'GnC_Cy
Cuts at: 2054 2426 2427 2464 2471 3088 2054
Size: 372 1 37 7 617 5144
Fragments arranged by size:
5144 617 372 37 7 1
EcoRI G'AATT_C
Cuts at: 2657 3487 3865 2657
Size: 830 378 4970
Fragments arranged by size:
4970 830 378
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 2012 2085 2354 5325 1678
Size: 334 73 269 2971 2531
Fragments arranged by size:
2971 2531 334 269 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2270 2302 2508
Size: 32 135 32 548 32 118 32 206
Cuts at: 2508 2540 4819 4851 4893 4925 1373
Size: 32 2279 32 42 32 2626
Fragments arranged by size:
2626 2279 548 206 135 118 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3522 3768 669
Size: 988 281 1584 246 3079
Fragments arranged by size:
3079 1584 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3407 748
Size: 2659 3519
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2509 5292 5476 1305
Size: 1204 2783 184 2007
Fragments arranged by size:
2783 2007 1204 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2181 2948 3050 10
Size: 54 1425 343 349 767 102 3138
Fragments arranged by size:
3138 1425 767 349 343 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 4136 4495 4654 518
Size: 648 2037 933 359 159 2042
Fragments arranged by size:
2042 2037 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2827 3064 3328 3399 3594 4271 5419
Size: 431 790 237 264 71 195 677 1148
Cuts at: 5419 5664 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 790 677 431 264 245 237
195 71
NaeI GCC'GGC
Cuts at: 2102 2102
Size: 6178
NcoI C'CATG_G
Cuts at: 513 1065 2004 2085 3441 513
Size: 552 939 81 1356 3250
Fragments arranged by size:
3250 1356 939 552 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NgoMIV G'CCGG_C
Cuts at: 2100 2100
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 3217 3536 5081 1132
Size: 97 1988 319 1545 2229
Fragments arranged by size:
2229 1988 1545 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 2467 2944 1108
Size: 1140 219 477 4342
Fragments arranged by size:
4342 1140 477 219
PfoI T'CCnGG_A
Cuts at: 2538 2538
Size: 6178
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2464 2464
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PspOMI G'GGCC_C
Cuts at: 2426 2470 2426
Size: 44 6134
PstI C_TGCA'G
Cuts at: 830 2216 2511 3075 3530 830
Size: 1386 295 564 455 3478
Fragments arranged by size:
3478 1386 564 455 295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
PvuII CAG'CTG
Cuts at: 2037 3064 2037
Size: 1027 5151
SacI G_AGCT'C
Cuts at: 3162 3336 3162
Size: 174 6004
SacII CC_GC'GG
Cuts at: 3329 3400 3329
Size: 71 6107
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SbfI CC_TGCA'GG
Cuts at: 3075 3530 3075
Size: 455 5723
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SexAI A'CCwGG_T
Cuts at: 3042 3042
Size: 6178
SfcI C'TryA_G
Cuts at: 826 1045 2212 2507 3071 3526 4661 5342 5533
Size: 219 1167 295 564 455 1135 681 191
Cuts at: 5533 826
Size: 1471
Fragments arranged by size:
1471 1167 1135 681 564 455 295 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2264 2264
Size: 6178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 2255 3357 3505 1872
Size: 383 1102 148 4545
Fragments arranged by size:
4545 1102 383 148
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3335 4282 5183 5445 820
Size: 197 926 210 1182 947 901 262 1553
Fragments arranged by size:
1553 1182 947 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SrfI GCCC'GGGC
Cuts at: 2255 2255
Size: 6178
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 3279 1517
Size: 1762 4416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2094 3000 3441 3474 3617
Size: 552 939 81 9 906 441 33 143
Cuts at: 3617 513
Size: 3074
Fragments arranged by size:
3074 939 906 552 441 143 81 33
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2614 2930 2988 4205 4222 4375 4560
Size: 1255 1034 316 58 1217 17 153 185
Cuts at: 4560 5005 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1217 1034 445 316 185 153
58 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4424 6136 96
Size: 274 80 33 51 1443 2447 1712 138
Fragments arranged by size:
2447 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 3265 3297 6138 6170 2133
Size: 32 1100 32 2841 32 2141
Fragments arranged by size:
2841 2141 1100 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2092 2174 2982 2092
Size: 82 808 5288
Fragments arranged by size:
5288 808 82
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 2253 3355 3503 1870
Size: 383 1102 148 4545
Fragments arranged by size:
4545 1102 383 148
XmnI GAAnn'nnTTC
Cuts at: 3389 4307 3389
Size: 918 5260
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SapI SfoI SwaI