Restriction map for
pFN21AB0444
(Circular) (Six-base) MAPSORT of: pFN21AB0444.seq Check: 90 from: 1 to: 7420
pFN21AB0444 7420 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2294 2989 2294
Size: 695 6725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5230 278
Size: 53 83 186 936 3694 2468
Fragments arranged by size:
3694 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4763 1937
Size: 2826 4594
Acc65I G'GTAC_C
Cuts at: 4741 4741
Size: 7420
AclI AA'CG_TT
Cuts at: 1563 5547 5920 1563
Size: 3984 373 3063
Fragments arranged by size:
3984 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2736 2907 3099 3432 3482 4082 4529 5483
Size: 1104 171 192 333 50 600 447 954
Cuts at: 5483 6867 7135 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1104 954 600 447 333 268
192 171 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7223
AflIII A'CryG_T
Cuts at: 1203 6319 7296 1203
Size: 5116 977 1327
Fragments arranged by size:
5116 1327 977
AgeI A'CCGG_T
Cuts at: 6251 6251
Size: 7420
AhdI GACnn_n'nnGTC
Cuts at: 4257 6149 4257
Size: 1892 5528
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3371 1184
Size: 2187 5233
AlwNI CAG_nnn'CTG
Cuts at: 2048 2220 2642 2981 5163 6735 2048
Size: 172 422 339 2182 1572 2733
Fragments arranged by size:
2733 2182 1572 422 339 172
ApaLI G'TGCA_C
Cuts at: 5477 6633 5477
Size: 1156 6264
ApoI r'AATT_y
Cuts at: 1493 2109 2435 2864 3032 3581 3608 4233 4412
Size: 616 326 429 168 549 27 625 179
Cuts at: 4412 4729 4955 5107 7216 1493
Size: 317 226 152 2109 1697
Fragments arranged by size:
2109 1697 625 616 549 429 326 317
226 179 168 152 27
AseI AT'TA_AT
Cuts at: 160 1032 5974 160
Size: 872 4942 1606
Fragments arranged by size:
4942 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
AvaI C'yCGr_G
Cuts at: 1870 1943 3062 4745 1870
Size: 73 1119 1683 4545
Fragments arranged by size:
4545 1683 1119 73
BamHI G'GATC_C
Cuts at: 4750 5117 4750
Size: 367 7053
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3281 3364 4657 4741 6196
Size: 325 217 627 1494 83 1293 84 1455
Cuts at: 6196 618
Size: 1842
Fragments arranged by size:
1842 1494 1455 1293 627 325 217 84
83
BanII G_rGCy'C
Cuts at: 1391 4739 1391
Size: 3348 4072
BbeI G_GCGC'C
Cuts at: 3285 3368 4661 3285
Size: 83 1293 6044
Fragments arranged by size:
6044 1293 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3666 3929 4617 928
Size: 971 1767 263 688 3731
Fragments arranged by size:
3731 1767 971 688 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5611 5645 1903
Size: 34 3674 34 3678
Fragments arranged by size:
3678 3674 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5312 6522 5312
Size: 1210 6210
BclI T'GATC_A
Cuts at: 1548 3738 1548
Size: 2190 5230
BfrBI ATG'CAT
Cuts at: 4665 4665
Size: 7420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3341 4292 6031
Size: 107 122 71 631 605 1669 951 1739
Cuts at: 6031 136
Size: 1525
Fragments arranged by size:
1739 1669 1525 951 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4363 7415 4363
Size: 3052 4368
BlpI GC'TnA_GC
Cuts at: 4837 4837
Size: 7420
Bme1580I G_kGCm'C
Cuts at: 1790 3205 3234 5481 6637 1790
Size: 1415 29 2247 1156 2573
Fragments arranged by size:
2573 2247 1415 1156 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7420
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4366 5151
Size: 416 53 83 399 24 1811 1138 785
Cuts at: 5151 6109 442
Size: 958 1753
Fragments arranged by size:
1811 1753 1138 958 785 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2025 6080 1239
Size: 786 4055 2579
Fragments arranged by size:
4055 2579 786
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3953 5545 6410 6708 3953
Size: 1592 865 298 4665
Fragments arranged by size:
4665 1592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6083 882
Size: 617 4584 2219
Fragments arranged by size:
4584 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3355 5211 493
Size: 711 2151 1856 2702
Fragments arranged by size:
2702 2151 1856 711
BsaBI GATnn'nnATC
Cuts at: 3433 7414 3433
Size: 3981 3439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3282 3365 4658 5227
Size: 53 83 186 936 1749 83 1293 569
Cuts at: 5227 5609 6244 275
Size: 382 635 1451
Fragments arranged by size:
1749 1451 1293 936 635 569 382 186
83 83 53
BsaWI w'CCGG_w
Cuts at: 5853 6251 6525 6672 7083 5853
Size: 398 274 147 411 6190
Fragments arranged by size:
6190 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7195
Fragments arranged by size:
7195 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2113 2603 1186
Size: 927 490 6003
Fragments arranged by size:
6003 927 490
BseYI C'CCAG_C
Cuts at: 2763 6623 7119 2763
Size: 3860 496 3064
Fragments arranged by size:
3860 3064 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 3711 1163
Size: 573 729 1246 4872
Fragments arranged by size:
4872 1246 729 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4870 5631 6659 1540
Size: 275 186 2869 761 1028 2301
Fragments arranged by size:
2869 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3254 5481 5566 6637 3254
Size: 2227 85 1071 4037
Fragments arranged by size:
4037 2227 1071 85
BsmI GAATG_Cn'
Cuts at: 4288 4931 5024 4288
Size: 643 93 6684
Fragments arranged by size:
6684 643 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4484 4484
Size: 7420
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3205 3234 3254 4739 5481 5566 6637
Size: 399 1415 29 20 1485 742 85 1071
Cuts at: 6637 1391
Size: 2174
Fragments arranged by size:
2174 1485 1415 1071 742 399 85 29
20
BspEI T'CCGG_A
Cuts at: 7083 7083
Size: 7420
BspHI T'CATG_A
Cuts at: 5309 7039 5309
Size: 1730 5690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 2989 3388 3849 4775 844
Size: 1450 695 399 461 926 3489
Fragments arranged by size:
3489 1450 926 695 461 399
BsrBI CCG'CTC
Cuts at: 1394 1823 5307 1394
Size: 429 3484 3507
Fragments arranged by size:
3507 3484 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3789 5915 6089 65
Size: 1183 2541 2126 174 1396
Fragments arranged by size:
2541 2126 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4707 6064 6251 1700
Size: 110 2897 1357 187 2869
Fragments arranged by size:
2897 2869 1357 187 110
BsrGI T'GTAC_A
Cuts at: 96 3705 96
Size: 3609 3811
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7420
BssSI C'ACGA_G
Cuts at: 3204 5480 6492 3204
Size: 2276 1012 4132
Fragments arranged by size:
4132 2276 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7420
BstBI TT'CG_AA
Cuts at: 6224 6224
Size: 7420
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7420
BstXI CCAn_nnnn'nTGG
Cuts at: 4693 4693
Size: 7420
BstYI r'GATC_y
Cuts at: 1972 4363 4750 5117 5502 5519 6971 7057 7069
Size: 2391 387 367 385 17 1452 86 12
Cuts at: 7069 7415 1972
Size: 346 1977
Fragments arranged by size:
2391 1977 1452 387 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3723 3723
Size: 7420
BtgI C'CryG_G
Cuts at: 513 1065 2004 3960 513
Size: 552 939 1956 3973
Fragments arranged by size:
3973 1956 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6681
Fragments arranged by size:
6681 683 56
BtsI GCAGTG_nn'
Cuts at: 2911 3048 3103 4217 4936 5730 5750 2911
Size: 137 55 1114 719 794 20 4581
Fragments arranged by size:
4581 1114 794 719 137 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4691 4726 529
Size: 35 4127 35 3223
Fragments arranged by size:
4127 3223 35 35
DraI TTT'AAA
Cuts at: 3586 4724 3586
Size: 1138 6282
DraIII CAC_nnn'GTG
Cuts at: 3400 3596 3400
Size: 196 7224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6427 809
Size: 507 5111 1802
Fragments arranged by size:
5111 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2706 2877 3045
Size: 54 1425 50 275 18 876 171 168
Cuts at: 3045 4867 5756 7385 8
Size: 1822 889 1629 43
Fragments arranged by size:
1822 1629 1425 889 876 275 171 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4867 1537
Size: 275 3055 4090
Fragments arranged by size:
4090 3055 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5350 1353
Size: 3997 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6007 6381 6527 387
Size: 1064 138 4418 374 146 1280
Fragments arranged by size:
4418 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2736 2907 3099 3432 3482 4082
Size: 393 393 711 171 192 333 50 600
Cuts at: 4082 4529 5483 6080 6867 7135 1239
Size: 447 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 711 600 597 447 393
393 333 268 192 171 50
EcoO109I rG'GnC_Cy
Cuts at: 2807 3312 2807
Size: 505 6915
EcoRI G'AATT_C
Cuts at: 4729 5107 4729
Size: 378 7042
EcoRV GAT'ATC
Cuts at: 3719 3719
Size: 7420
FspI TGC'GCA
Cuts at: 5926 5926
Size: 7420
HaeII r_GCGC'y
Cuts at: 1678 3285 3368 4661 6567 1678
Size: 1607 83 1293 1906 2531
Fragments arranged by size:
2531 1906 1607 1293 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2016 2048 3716 3748 3973
Size: 32 135 32 444 32 1668 32 225
Cuts at: 3973 4005 4243 4275 6061 6093 6135 6167 1373
Size: 32 238 32 1786 32 42 32 2626
Fragments arranged by size:
2626 1786 1668 444 238 225 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3844 4764 5010 669
Size: 988 281 1906 920 246 3079
Fragments arranged by size:
3079 1906 988 920 281 246
HindIII A'AGCT_T
Cuts at: 748 3182 748
Size: 2434 4986
HpaI GTT'AAC
Cuts at: 5010 5010
Size: 7420
KasI G'GCGC_C
Cuts at: 3281 3364 4657 3281
Size: 83 1293 6044
Fragments arranged by size:
6044 1293 83
KpnI G_GTAC'C
Cuts at: 4745 4745
Size: 7420
MfeI C'AATT_G
Cuts at: 4134 5019 4134
Size: 885 6535
MluI A'CGCG_T
Cuts at: 7296 7296
Size: 7420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3174 6534 6718 1305
Size: 1869 3360 184 2007
Fragments arranged by size:
3360 2007 1869 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2708 2879 3047 10
Size: 54 1425 343 876 171 168 4383
Fragments arranged by size:
4383 1425 876 343 171 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5378 5737 5896 518
Size: 648 4212 359 159 2042
Fragments arranged by size:
4212 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2548 3410 4836 5513 6661 6906 1606
Size: 942 862 1426 677 1148 245 2120
Fragments arranged by size:
2120 1426 1148 942 862 677 245
NarI GG'CG_CC
Cuts at: 3282 3365 4658 3282
Size: 83 1293 6044
Fragments arranged by size:
6044 1293 83
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5929
Fragments arranged by size:
5929 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7420
NotI GC'GGCC_GC
Cuts at: 4867 4867
Size: 7420
NsiI A_TGCA'T
Cuts at: 4667 4667
Size: 7420
NspI r_CATG'y
Cuts at: 1132 1229 2462 4778 6323 1132
Size: 97 1233 2316 1545 2229
Fragments arranged by size:
2316 2229 1545 1233 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7420
PciI A'CATG_T
Cuts at: 6319 6319
Size: 7420
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4203 1108
Size: 3095 4325
PmeI GTTT'AAAC
Cuts at: 4724 4724
Size: 7420
PmlI CAC'GTG
Cuts at: 3355 3355
Size: 7420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5489 5521 7002 7034 5489
Size: 32 1481 32 5875
Fragments arranged by size:
5875 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2807 2807
Size: 7420
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7420
PsiI TTA'TAA
Cuts at: 2988 4990 2988
Size: 2002 5418
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2394 2426 2394
Size: 32 7388
PstI C_TGCA'G
Cuts at: 830 2968 3442 4567 4772 830
Size: 2138 474 1125 205 3478
Fragments arranged by size:
3478 2138 1125 474 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7420
PvuII CAG'CTG
Cuts at: 3410 3410
Size: 7420
SalI G'TCGA_C
Cuts at: 1936 4762 1936
Size: 2826 4594
SbfI CC_TGCA'GG
Cuts at: 4772 4772
Size: 7420
ScaI AGT'ACT
Cuts at: 4690 5668 7380 4690
Size: 978 1712 4730
Fragments arranged by size:
4730 1712 978
SfcI C'TryA_G
Cuts at: 826 1045 2964 3438 4563 4768 5903 6584 6775
Size: 219 1919 474 1125 205 1135 681 191
Cuts at: 6775 826
Size: 1471
Fragments arranged by size:
1919 1471 1135 1125 681 474 219 205
191
SfoI GGC'GCC
Cuts at: 3283 3366 4659 3283
Size: 83 1293 6044
Fragments arranged by size:
6044 1293 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7420
SmaI CCC'GGG
Cuts at: 1872 4747 1872
Size: 2875 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3062 3968 5524 6425 6687 820
Size: 197 926 1119 906 1556 901 262 1553
Fragments arranged by size:
1556 1553 1119 926 906 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5211 493
Size: 4718 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7420
SphI G_CATG'C
Cuts at: 1132 4778 1132
Size: 3646 3774
SspI AAT'ATT
Cuts at: 5 52 2338 5344 5
Size: 47 2286 3006 2081
Fragments arranged by size:
3006 2286 2081 47
StuI AGG'CCT
Cuts at: 1517 3335 1517
Size: 1818 5602
StyI C'CwwG_G
Cuts at: 513 1065 2004 3368 3907 4296 4702 4859 513
Size: 552 939 1364 539 389 406 157 3074
Fragments arranged by size:
3074 1364 939 552 539 406 389 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4585 5447 5464 5617 5802 6247 325
Size: 1255 3005 862 17 153 185 445 1498
Fragments arranged by size:
3005 1498 1255 862 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3122 3155 3705
Size: 274 80 33 51 1443 1145 33 550
Cuts at: 3705 4688 5666 7378 96
Size: 983 978 1712 138
Fragments arranged by size:
1712 1443 1145 983 978 550 274 138
80 51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2883 2915 3075 3107 7380 7412 2883
Size: 32 160 32 4273 32 2891
Fragments arranged by size:
4273 2891 160 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7420
XbaI T'CTAG_A
Cuts at: 4756 4756
Size: 7420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3905 3905
Size: 7420
XhoI C'TCGA_G
Cuts at: 1943 3062 1943
Size: 1119 6301
XmaI C'CCGG_G
Cuts at: 1870 4745 1870
Size: 2875 4545
XmnI GAAnn'nnTTC
Cuts at: 3663 3833 4056 5549 3663
Size: 170 223 1493 5534
Fragments arranged by size:
5534 1493 223 170
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5228 276
Size: 53 83 186 936 3694 2468
Fragments arranged by size:
3694 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI
NaeI NgoMIV NruI PacI PfoI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI