Restriction map for
pFN21AB0442
(Circular) (Six-base) MAPSORT of: pFN21AB0442.seq Check: 6794 from: 1 to: 7411
pFN21AB0442 7411 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4345 4345
Size: 7411
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5221 278
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3996 4754 1937
Size: 2059 758 4594
Fragments arranged by size:
4594 2059 758
Acc65I G'GTAC_C
Cuts at: 4313 4732 4313
Size: 419 6992
AclI AA'CG_TT
Cuts at: 1563 5538 5911 1563
Size: 3975 373 3063
Fragments arranged by size:
3975 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2488 2823 3181 3605 4095 5474 6858 7126
Size: 856 335 358 424 490 1379 1384 268
Cuts at: 7126 1632
Size: 1917
Fragments arranged by size:
1917 1384 1379 856 490 424 358 335
268
AfeI AGC'GCT
Cuts at: 2394 3743 2394
Size: 1349 6062
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7214
AflIII A'CryG_T
Cuts at: 1203 3647 3649 3673 6310 7287 1203
Size: 2444 2 24 2637 977 1327
Fragments arranged by size:
2637 2444 1327 977 24 2
AgeI A'CCGG_T
Cuts at: 6242 6242
Size: 7411
AhdI GACnn_n'nnGTC
Cuts at: 6140 6140
Size: 7411
AleI CACnn'nnGTG
Cuts at: 1166 3518 1166
Size: 2352 5059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7411
AlwNI CAG_nnn'CTG
Cuts at: 3757 3789 5154 6726 3757
Size: 32 1365 1572 4442
Fragments arranged by size:
4442 1572 1365 32
ApaI G_GGCC'C
Cuts at: 2769 2965 3298 4266 2769
Size: 196 333 968 5914
Fragments arranged by size:
5914 968 333 196
ApaLI G'TGCA_C
Cuts at: 3455 5468 6624 3455
Size: 2013 1156 4242
Fragments arranged by size:
4242 2013 1156
ApoI r'AATT_y
Cuts at: 1493 2039 3595 4720 4946 5098 7207 1493
Size: 546 1556 1125 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 1125 546 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5965 160
Size: 872 4933 1606
Fragments arranged by size:
4933 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
AvaI C'yCGr_G
Cuts at: 1870 1943 2336 2658 2824 2922 2929 3956 4736
Size: 73 393 322 166 98 7 1027 780
Cuts at: 4736 1870
Size: 4545
Fragments arranged by size:
4545 1027 780 393 322 166 98 73
7
AvrII C'CTAG_G
Cuts at: 3367 3367
Size: 7411
BamHI G'GATC_C
Cuts at: 4741 5108 4741
Size: 367 7044
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 3512 3721 4313 4732
Size: 325 217 627 465 1260 209 592 419
Cuts at: 4732 6187 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1260 627 592 465 419 325
217 209
BanII G_rGCy'C
Cuts at: 1391 2157 2642 2769 2936 2965 3286 3298 4080
Size: 766 485 127 167 29 321 12 782
Cuts at: 4080 4266 4394 4564 4730 1391
Size: 186 128 170 166 4072
Fragments arranged by size:
4072 782 766 485 321 186 170 167
166 128 127 29 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2996 4194 928
Size: 971 1097 1198 4145
Fragments arranged by size:
4145 1198 1097 971
BbvCI CC'TCA_GC
Cuts at: 3260 4515 3260
Size: 1255 6156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5602 5636 1903
Size: 34 3665 34 3678
Fragments arranged by size:
3678 3665 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4514 5303 6513 4514
Size: 789 1210 5412
Fragments arranged by size:
5412 1210 789
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7411
BfrBI ATG'CAT
Cuts at: 2218 2218
Size: 7411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2721 6022
Size: 107 122 71 631 605 337 712 3301
Cuts at: 6022 136
Size: 1525
Fragments arranged by size:
3301 1525 712 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 7406 7406
Size: 7411
BlpI GC'TnA_GC
Cuts at: 3745 4828 3745
Size: 1083 6328
Bme1580I G_kGCm'C
Cuts at: 1790 2769 2965 3147 3298 3459 3681 3726 4266
Size: 979 196 182 151 161 222 45 540
Cuts at: 4266 4330 5472 6628 1790
Size: 64 1142 1156 2573
Fragments arranged by size:
2573 1156 1142 979 540 222 196 182
161 151 64 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3777 5142 6100
Size: 416 53 83 399 24 2360 1365 958
Cuts at: 6100 442
Size: 1753
Fragments arranged by size:
2360 1753 1365 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7411
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 3000 3064 2638
Size: 362 64 6985
Fragments arranged by size:
6985 362 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2716 3081 3275 4658 4713 6071 1239
Size: 1178 299 365 194 1383 55 1358 2579
Fragments arranged by size:
2579 1383 1358 1178 365 299 194 55
Bpu10I CC'TnA_GC
Cuts at: 3260 4515 3260
Size: 1255 6156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5536 6401 6699 5536
Size: 865 298 6248
Fragments arranged by size:
6248 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4659 6074 882
Size: 617 3160 1415 2219
Fragments arranged by size:
3160 2219 1415 617
BsaAI yAC'GTr
Cuts at: 493 1204 3650 3676 5202 493
Size: 711 2446 26 1526 2702
Fragments arranged by size:
2702 2446 1526 711 26
BsaBI GATnn'nnATC
Cuts at: 3404 4500 7405 3404
Size: 1096 2905 3410
Fragments arranged by size:
3410 2905 1096
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5218 5600 6235 275
Size: 53 83 186 936 3685 382 635 1451
Fragments arranged by size:
3685 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4084 4564 5844 6242 6516 6663 7074 4084
Size: 480 1280 398 274 147 411 4421
Fragments arranged by size:
4421 1280 480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2918 2948 1178
Size: 30 165 30 1515 30 5641
Fragments arranged by size:
5641 1515 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2741 3053 3084 3095 3816 3911 3914
Size: 859 696 312 31 11 721 95 3
Cuts at: 3914 4088 4384 4437 1186
Size: 174 296 53 4160
Fragments arranged by size:
4160 859 721 696 312 296 174 95
53 31 11 3
BseYI C'CCAG_C
Cuts at: 2080 2287 2309 2318 2345 2773 2848 3236 3738
Size: 207 22 9 27 428 75 388 502
Cuts at: 3738 6614 7110 2080
Size: 2876 496 2381
Fragments arranged by size:
2876 2381 502 496 428 388 207 75
27 22 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2157 4668 1163
Size: 573 421 2511 3906
Fragments arranged by size:
3906 2511 573 421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4442 4861 5622 6650 1540
Size: 275 186 2441 419 761 1028 2301
Fragments arranged by size:
2441 2301 1028 761 419 275 186
BsiHKAI G_wGCw'C
Cuts at: 2761 3459 3737 5472 5557 6628 2761
Size: 698 278 1735 85 1071 3544
Fragments arranged by size:
3544 1735 1071 698 278 85
BsmI GAATG_Cn'
Cuts at: 2218 4922 5015 2218
Size: 2704 93 4614
Fragments arranged by size:
4614 2704 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3617 3706 3617
Size: 89 7322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2642 2761 2769 2936 2965 3147
Size: 399 367 485 119 8 167 29 182
Cuts at: 3147 3286 3298 3459 3681 3726 3737 4080 4266
Size: 139 12 161 222 45 11 343 186
Cuts at: 4266 4330 4394 4564 4730 5472 5557 6628 1391
Size: 64 64 170 166 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 367 343 222
186 182 170 167 166 161 139 119
85 64 64 45 29 12 11 8
BspEI T'CCGG_A
Cuts at: 4564 7074 4564
Size: 2510 4901
BspHI T'CATG_A
Cuts at: 5300 7030 5300
Size: 1730 5681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3438 4345 4519 4766 844
Size: 2594 907 174 247 3489
Fragments arranged by size:
3489 2594 907 247 174
BsrBI CCG'CTC
Cuts at: 1394 1823 5298 1394
Size: 429 3475 3507
Fragments arranged by size:
3507 3475 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2909 3061 3896 5906 6080 65
Size: 1183 1661 152 835 2010 174 1396
Fragments arranged by size:
2010 1661 1396 1183 835 174 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 6055 6242 1700
Size: 110 4245 187 2869
Fragments arranged by size:
4245 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7411
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7411
BssSI C'ACGA_G
Cuts at: 2536 5471 6483 2536
Size: 2935 1012 3464
Fragments arranged by size:
3464 2935 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7411
BstBI TT'CG_AA
Cuts at: 6215 6215
Size: 7411
BstEII G'GTnAC_C
Cuts at: 1184 3347 1184
Size: 2163 5248
BstXI CCAn_nnnn'nTGG
Cuts at: 3159 3225 3523 3159
Size: 66 298 7047
Fragments arranged by size:
7047 298 66
BstYI r'GATC_y
Cuts at: 1972 2689 4741 5108 5493 5510 6962 7048 7060
Size: 717 2052 367 385 17 1452 86 12
Cuts at: 7060 7406 1972
Size: 346 1977
Fragments arranged by size:
2052 1977 1452 717 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2675 2783 2863 3319 3483 513
Size: 552 939 671 108 80 456 164 4441
Fragments arranged by size:
4441 939 671 552 456 164 108 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6672
Fragments arranged by size:
6672 683 56
BtsI GCAGTG_nn'
Cuts at: 3024 3147 3411 4927 5721 5741 3024
Size: 123 264 1516 794 20 4694
Fragments arranged by size:
4694 1516 794 264 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2892 2927 529
Size: 35 2328 35 5013
Fragments arranged by size:
5013 2328 35 35
DraI TTT'AAA
Cuts at: 4715 4715
Size: 7411
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6418 809
Size: 507 5102 1802
Fragments arranged by size:
5102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2326 2672
Size: 54 1425 50 275 18 183 313 346
Cuts at: 2672 3480 3486 3558 3983 4439 4660 4858 5747
Size: 808 6 72 425 456 221 198 889
Cuts at: 5747 7376 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 808 456 425 346 313
275 221 198 183 72 54 50 43
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 4439 4858 1537
Size: 275 2627 419 4090
Fragments arranged by size:
4090 2627 419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2874 4455 4692 5341 1353
Size: 1521 1581 237 649 3423
Fragments arranged by size:
3423 1581 1521 649 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3013 4033 4413 5998 6372 6518
Size: 1064 138 1424 1020 380 1585 374 146
Cuts at: 6518 387
Size: 1280
Fragments arranged by size:
1585 1424 1280 1064 1020 380 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2488 2716 2823 3081 3181 3275
Size: 393 785 71 228 107 258 100 94
Cuts at: 3275 3605 4095 4658 4713 5474 6071 6858 7126
Size: 330 490 563 55 761 597 787 268
Cuts at: 7126 1239
Size: 1524
Fragments arranged by size:
1524 787 785 761 597 563 490 393
330 268 258 228 107 100 94 71
55
EcoNI CCTnn'n_nnAGG
Cuts at: 3099 3099
Size: 7411
EcoO109I rG'GnC_Cy
Cuts at: 2071 2496 2926 2961 2962 3201 3872 4161 4262
Size: 425 430 35 1 239 671 289 101
Cuts at: 4262 4263 4475 2071
Size: 1 212 5007
Fragments arranged by size:
5007 671 430 425 289 239 212 101
35 1 1
EcoRI G'AATT_C
Cuts at: 3595 4720 5098 3595
Size: 1125 378 5908
Fragments arranged by size:
5908 1125 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 7411
FspI TGC'GCA
Cuts at: 5917 5917
Size: 7411
HaeII r_GCGC'y
Cuts at: 1678 2172 2396 2815 3745 6558 1678
Size: 494 224 419 930 2813 2531
Fragments arranged by size:
2813 2531 930 494 419 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 2918 2950 4599
Size: 32 135 32 1013 32 301 32 1649
Cuts at: 4599 4631 6052 6084 6126 6158 1373
Size: 32 1421 32 42 32 2626
Fragments arranged by size:
2626 1649 1421 1013 301 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4755 5001 669
Size: 988 281 2817 246 3079
Fragments arranged by size:
3079 2817 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3587 748
Size: 2839 4572
HpaI GTT'AAC
Cuts at: 5001 5001
Size: 7411
KpnI G_GTAC'C
Cuts at: 4317 4736 4317
Size: 419 6992
MfeI C'AATT_G
Cuts at: 5010 5010
Size: 7411
MluI A'CGCG_T
Cuts at: 7287 7287
Size: 7411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3504 3855 6525 6709 1305
Size: 2199 351 2670 184 2007
Fragments arranged by size:
2670 2199 2007 351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3482 10
Size: 54 1425 343 1650 3939
Fragments arranged by size:
3939 1650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3518 4139 5369 5728 5887 518
Size: 648 2352 621 1230 359 159 2042
Fragments arranged by size:
2352 2042 1230 648 621 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2080 2647 2785 2972 3253 4599 4827
Size: 413 61 567 138 187 281 1346 228
Cuts at: 4827 5504 6652 6897 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1346 1148 677 567 413 281 245
228 187 138 61
NcoI C'CATG_G
Cuts at: 513 1065 2004 2675 513
Size: 552 939 671 5249
Fragments arranged by size:
5249 939 671 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7411
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7411
NotI GC'GGCC_GC
Cuts at: 4858 4858
Size: 7411
NsiI A_TGCA'T
Cuts at: 2220 2220
Size: 7411
NspI r_CATG'y
Cuts at: 1132 1229 2218 4240 4769 6314 1132
Size: 97 989 2022 529 1545 2229
Fragments arranged by size:
2229 2022 1545 989 529 97
PasI CC'CwG_GG
Cuts at: 1348 3682 4405 4550 1348
Size: 2334 723 145 4209
Fragments arranged by size:
4209 2334 723 145
PciI A'CATG_T
Cuts at: 6310 6310
Size: 7411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2516 4544 1108
Size: 1408 2028 3975
Fragments arranged by size:
3975 2028 1408
PfoI T'CCnGG_A
Cuts at: 3097 3107 4690 3097
Size: 10 1583 5818
Fragments arranged by size:
5818 1583 10
PmeI GTTT'AAAC
Cuts at: 4715 4715
Size: 7411
PmlI CAC'GTG
Cuts at: 3650 3676 3650
Size: 26 7385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5480 5512 6993 7025 5480
Size: 32 1481 32 5866
Fragments arranged by size:
5866 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2926 3201 3872 2926
Size: 275 671 6465
Fragments arranged by size:
6465 671 275
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7411
PsiI TTA'TAA
Cuts at: 4981 4981
Size: 7411
PspOMI G'GGCC_C
Cuts at: 2765 2961 3294 4262 2765
Size: 196 333 968 5914
Fragments arranged by size:
5914 968 333 196
PstI C_TGCA'G
Cuts at: 830 3418 3924 4342 4763 830
Size: 2588 506 418 421 3478
Fragments arranged by size:
3478 2588 506 421 418
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7411
PvuII CAG'CTG
Cuts at: 2019 2972 3253 2019
Size: 953 281 6177
Fragments arranged by size:
6177 953 281
SacII CC_GC'GG
Cuts at: 2786 2786
Size: 7411
SalI G'TCGA_C
Cuts at: 1936 4753 1936
Size: 2817 4594
SanDI GG'GwC_CC
Cuts at: 2926 2926
Size: 7411
SapI GCTCTTCn'nnn_
Cuts at: 4692 4692
Size: 7411
SbfI CC_TGCA'GG
Cuts at: 4763 4763
Size: 7411
ScaI AGT'ACT
Cuts at: 5659 7371 5659
Size: 1712 5699
SfcI C'TryA_G
Cuts at: 826 1045 2467 3373 3395 3414 3775 3920 4276
Size: 219 1422 906 22 19 361 145 356
Cuts at: 4276 4338 4591 4759 5894 6575 6766 826
Size: 62 253 168 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 906 681 361 356 253
219 191 168 145 62 22 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7411
SmaI CCC'GGG
Cuts at: 1872 2660 2924 2931 4738 1872
Size: 788 264 7 1807 4545
Fragments arranged by size:
4545 1807 788 264 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 5515 6416 6678 820
Size: 197 926 393 3179 901 262 1553
Fragments arranged by size:
3179 1553 926 901 393 262 197
SnaBI TAC'GTA
Cuts at: 493 5202 493
Size: 4709 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7411
SphI G_CATG'C
Cuts at: 1132 2218 4240 4769 1132
Size: 1086 2022 529 3774
Fragments arranged by size:
3774 2022 1086 529
SspI AAT'ATT
Cuts at: 5 52 5335 5
Size: 47 5283 2081
Fragments arranged by size:
5283 2081 47
StuI AGG'CCT
Cuts at: 1517 3773 1517
Size: 2256 5155
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2675 3069 3165 3367 3516
Size: 552 939 141 530 394 96 202 149
Cuts at: 3516 3849 4850 513
Size: 333 1001 3074
Fragments arranged by size:
3074 1001 939 552 530 394 333 202
149 141 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3021 4171 5438 5455 5608 5793 6238
Size: 1255 1441 1150 1267 17 153 185 445
Cuts at: 6238 325
Size: 1498
Fragments arranged by size:
1498 1441 1267 1255 1150 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3706 3960 5657
Size: 274 80 33 51 1443 1729 254 1697
Cuts at: 5657 7369 96
Size: 1712 138
Fragments arranged by size:
1729 1712 1697 1443 274 254 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7371 7403 7371
Size: 32 7379
Tth111I GACn'n_nGTC
Cuts at: 1316 4613 1316
Size: 3297 4114
XbaI T'CTAG_A
Cuts at: 4747 4747
Size: 7411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2739 2739
Size: 7411
XhoI C'TCGA_G
Cuts at: 1943 2336 1943
Size: 393 7018
XmaI C'CCGG_G
Cuts at: 1870 2658 2922 2929 4736 1870
Size: 788 264 7 1807 4545
Fragments arranged by size:
4545 1807 788 264 7
XmnI GAAnn'nnTTC
Cuts at: 5540 5540
Size: 7411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5219 276
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspXI PsrI RsrII SacI SexAI SfiI
SfoI SrfI SwaI