Restriction map for
pFN21AB0441
(Circular) (Six-base) MAPSORT of: pFN21AB0441.seq Check: 9896 from: 1 to: 6037
pFN21AB0441 6037 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3116 3847 278
Size: 53 83 186 936 1580 731 2468
Fragments arranged by size:
2468 1580 936 731 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3380 1937
Size: 1443 4594
Acc65I G'GTAC_C
Cuts at: 2232 3358 2232
Size: 1126 4911
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2855 4100 5484 5752 1632
Size: 1223 1245 1384 268 1917
Fragments arranged by size:
1917 1384 1245 1223 268
AfeI AGC'GCT
Cuts at: 3070 3070
Size: 6037
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 4936 5913 1203
Size: 3733 977 1327
Fragments arranged by size:
3733 1327 977
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 2047 2289 4766 2047
Size: 242 2477 3318
Fragments arranged by size:
3318 2477 242
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6037
AlwNI CAG_nnn'CTG
Cuts at: 2537 3780 5352 2537
Size: 1243 1572 3222
Fragments arranged by size:
3222 1572 1243
ApaI G_GGCC'C
Cuts at: 2029 2029
Size: 6037
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 2167 2855 3346 3572 3724 5833 1493
Size: 674 688 491 226 152 2109 1697
Fragments arranged by size:
2109 1697 688 674 491 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 3362 1870
Size: 73 293 1126 4545
Fragments arranged by size:
4545 1126 293 73
AvrII C'CTAG_G
Cuts at: 2197 2197
Size: 6037
BamHI G'GATC_C
Cuts at: 3367 3734 3367
Size: 367 5670
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 2725 3358 4813 618
Size: 325 217 627 445 493 633 1455 1842
Fragments arranged by size:
1842 1455 633 627 493 445 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 2305 2899 3356 1391
Size: 638 276 594 457 4072
Fragments arranged by size:
4072 638 594 457 276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5066
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4228 4262 1903
Size: 34 2291 34 3678
Fragments arranged by size:
3678 2291 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 2595 1548
Size: 1047 4990
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 2796 3272
Size: 107 122 71 631 605 637 487 476
Cuts at: 3272 4648 136
Size: 1376 1525
Fragments arranged by size:
1525 1376 637 631 605 487 476 122
107 71
BglII A'GATC_T
Cuts at: 6032 6032
Size: 6037
BlpI GC'TnA_GC
Cuts at: 3454 3454
Size: 6037
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2730 4098 5254 1790
Size: 239 701 1368 1156 2573
Fragments arranged by size:
2573 1368 1156 701 239
BmgBI CAC'GTC
Cuts at: 1318 2493 1318
Size: 1175 4862
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 3768 4726
Size: 416 53 83 399 24 1658 693 958
Cuts at: 4726 442
Size: 1753
Fragments arranged by size:
1753 1658 958 693 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2582 4697 1239
Size: 1061 282 2115 2579
Fragments arranged by size:
2579 2115 1061 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4162 5027 5325 4162
Size: 865 298 4874
Fragments arranged by size:
4874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2276 4700 882
Size: 617 777 2424 2219
Fragments arranged by size:
2424 2219 777 617
BsaAI yAC'GTr
Cuts at: 493 1204 3828 493
Size: 711 2624 2702
Fragments arranged by size:
2702 2624 711
BsaBI GATnn'nnATC
Cuts at: 6031 6031
Size: 6037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3113 3844 4226 4861
Size: 53 83 186 936 1580 731 382 635
Cuts at: 4861 275
Size: 1451
Fragments arranged by size:
1580 1451 936 731 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2434 4470 4868 5142 5289 5700 2434
Size: 2036 398 274 147 411 2771
Fragments arranged by size:
2771 2036 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 1178
Size: 30 165 30 945 30 4837
Fragments arranged by size:
4837 945 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 3299 1186
Size: 1393 720 3924
Fragments arranged by size:
3924 1393 720
BseYI C'CCAG_C
Cuts at: 2334 2920 3094 3103 5240 5736 2334
Size: 586 174 9 2137 496 2635
Fragments arranged by size:
2635 2137 586 496 174 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3290 1163
Size: 573 1554 3910
Fragments arranged by size:
3910 1554 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3267 3487 4248 5276 1540
Size: 275 186 1266 220 761 1028 2301
Fragments arranged by size:
2301 1266 1028 761 275 220 186
BsiHKAI G_wGCw'C
Cuts at: 4098 4183 5254 4098
Size: 85 1071 4881
Fragments arranged by size:
4881 1071 85
BsmI GAATG_Cn'
Cuts at: 3548 3641 3548
Size: 93 5944
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2305 2730 2899 3356 4098 4183
Size: 399 239 276 425 169 457 742 85
Cuts at: 4183 5254 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 457 425 399 276 239
169 85
BspEI T'CCGG_A
Cuts at: 2434 5700 2434
Size: 3266 2771
BspHI T'CATG_A
Cuts at: 2463 3926 5656 2463
Size: 1463 1730 2844
Fragments arranged by size:
2844 1730 1463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3392 844
Size: 2548 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3924 1394
Size: 429 2101 3507
Fragments arranged by size:
3507 2101 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4532 4706 65
Size: 1183 3284 174 1396
Fragments arranged by size:
3284 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 4681 4868 1700
Size: 110 320 2551 187 2869
Fragments arranged by size:
2869 2551 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 2385 96
Size: 2289 3748
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 4097 5109 4097
Size: 1012 5025
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2378 794
Size: 1584 4453
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 2755 1184
Size: 1571 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 2334 2334
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2971 3189 3367 3734 4119 4136 5588 5674
Size: 999 218 178 367 385 17 1452 86
Cuts at: 5674 5686 6032 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 999 385 367 346 218 178
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2407 2455 3118 513
Size: 552 1342 48 663 3432
Fragments arranged by size:
3432 1342 663 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2584 487
Size: 683 56 1358 3940
Fragments arranged by size:
3940 1358 683 56
BtsI GCAGTG_nn'
Cuts at: 2388 2743 3553 4347 4367 2388
Size: 355 810 794 20 4058
Fragments arranged by size:
4058 810 794 355 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 2860 3341 2860
Size: 481 5556
DraIII CAC_nnn'GTG
Cuts at: 2878 2878
Size: 6037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3206 5044 809
Size: 507 1890 1838 1802
Fragments arranged by size:
1890 1838 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2883 3264 3484
Size: 54 1425 50 275 18 1053 381 220
Cuts at: 3484 4373 6002 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1053 889 381 275 220 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3264 3484 1537
Size: 275 1452 220 4090
Fragments arranged by size:
4090 1452 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2585 3314 3967 1353
Size: 1232 729 653 3423
Fragments arranged by size:
3423 1232 729 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2699 4624 4998 5144 387
Size: 1064 138 1110 1925 374 146 1280
Fragments arranged by size:
1925 1280 1110 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2582 2855 4100 4697 5484 5752
Size: 393 668 282 273 1245 597 787 268
Cuts at: 5752 1239
Size: 1524
Fragments arranged by size:
1524 1245 787 668 597 393 282 273
268
EcoO109I rG'GnC_Cy
Cuts at: 2025 2194 2908 3099 2025
Size: 169 714 191 4963
Fragments arranged by size:
4963 714 191 169
EcoRI G'AATT_C
Cuts at: 3346 3724 3346
Size: 378 5659
FspI TGC'GCA
Cuts at: 4543 4543
Size: 6037
HaeII r_GCGC'y
Cuts at: 1678 3072 5184 1678
Size: 1394 2112 2531
Fragments arranged by size:
2531 2112 1394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2275 2307 4678
Size: 32 135 32 461 32 210 32 2371
Cuts at: 4678 4710 4752 4784 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2371 461 210 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2020 2344 3381 3627 669
Size: 988 281 82 324 1037 246 3079
Fragments arranged by size:
3079 1037 988 324 281 246 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KpnI G_GTAC'C
Cuts at: 2236 3362 2236
Size: 1126 4911
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2122 5151 5335 1305
Size: 817 3029 184 2007
Fragments arranged by size:
3029 2007 817 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2885 10
Size: 54 1425 343 1053 3162
Fragments arranged by size:
3162 1425 1053 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2034 2748 3995 4354 4513 518
Size: 648 843 25 714 1247 359 159 2042
Fragments arranged by size:
2042 1247 843 714 648 359 159 25
MspA1I CmG'CkG
Cuts at: 1606 2560 2613 2703 3453 4130 5278 5523 1606
Size: 954 53 90 750 677 1148 245 2120
Fragments arranged by size:
2120 1148 954 750 677 245 90 53
NcoI C'CATG_G
Cuts at: 513 1065 2407 2455 513
Size: 552 1342 48 4095
Fragments arranged by size:
4095 1342 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 3395 4940 1132
Size: 97 2166 1545 2229
Fragments arranged by size:
2229 2166 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 4936 4936
Size: 6037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2537 2881 1108
Size: 1429 344 4264
Fragments arranged by size:
4264 1429 344
PfoI T'CCnGG_A
Cuts at: 3279 3279
Size: 6037
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4106 4138 5619 5651 4106
Size: 32 1481 32 4492
Fragments arranged by size:
4492 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3099 3099
Size: 6037
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 3607 3607
Size: 6037
PspOMI G'GGCC_C
Cuts at: 2025 2025
Size: 6037
PstI C_TGCA'G
Cuts at: 830 2310 2894 3189 3389 830
Size: 1480 584 295 200 3478
Fragments arranged by size:
3478 1480 584 295 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
PvuII CAG'CTG
Cuts at: 2560 2613 2560
Size: 53 5984
SalI G'TCGA_C
Cuts at: 1936 3379 1936
Size: 1443 4594
SanDI GG'GwC_CC
Cuts at: 3099 3099
Size: 6037
SbfI CC_TGCA'GG
Cuts at: 2310 3389 2310
Size: 1079 4958
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SexAI A'CCwGG_T
Cuts at: 2874 2874
Size: 6037
SfcI C'TryA_G
Cuts at: 826 1045 2306 2809 2890 3185 3385 4520 5201
Size: 219 1261 503 81 295 200 1135 681
Cuts at: 5201 5392 826
Size: 191 1471
Fragments arranged by size:
1471 1261 1135 681 503 295 219 200
191 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 2238 3364 1872
Size: 366 1126 4545
Fragments arranged by size:
4545 1126 366
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4141 5042 5304 820
Size: 197 926 2198 901 262 1553
Fragments arranged by size:
2198 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 3961 5
Size: 47 3909 2081
Fragments arranged by size:
3909 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 1517
Size: 795 5242
StyI C'CwwG_G
Cuts at: 513 1065 2197 2407 2455 3476 513
Size: 552 1132 210 48 1021 3074
Fragments arranged by size:
3074 1132 1021 552 210 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4064 4081 4234 4419 4864 325
Size: 1255 2484 17 153 185 445 1498
Fragments arranged by size:
2484 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 4283 5995
Size: 274 80 33 51 1443 408 1898 1712
Cuts at: 5995 96
Size: 138
Fragments arranged by size:
1898 1712 1443 408 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2565 2597 5997 6029 2315
Size: 32 218 32 3400 32 2323
Fragments arranged by size:
3400 2323 218 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6037
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2249 2453 3200 2249
Size: 204 747 5086
Fragments arranged by size:
5086 747 204
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 2236 3362 1870
Size: 366 1126 4545
Fragments arranged by size:
4545 1126 366
XmnI GAAnn'nnTTC
Cuts at: 3227 4166 3227
Size: 939 5098
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3114 3845 276
Size: 53 83 186 936 1580 731 2468
Fragments arranged by size:
2468 1580 936 731 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI