Restriction map for
pFN21AB0438
(Circular) (Six-base) MAPSORT of: pFN21AB0438.seq Check: 4370 from: 1 to: 7312
pFN21AB0438 7312 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2995 3054 4357 2995
Size: 59 1303 5950
Fragments arranged by size:
5950 1303 59
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2070 5122 278
Size: 53 83 186 936 534 3052 2468
Fragments arranged by size:
3052 2468 936 534 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2694 4655 1937
Size: 757 1961 4594
Fragments arranged by size:
4594 1961 757
Acc65I G'GTAC_C
Cuts at: 2274 4633 2274
Size: 2359 4953
AclI AA'CG_TT
Cuts at: 1563 5439 5812 1563
Size: 3876 373 3063
Fragments arranged by size:
3876 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3458 3944 5375 6759 7027 1632
Size: 1826 486 1431 1384 268 1917
Fragments arranged by size:
1917 1826 1431 1384 486 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7115
AflIII A'CryG_T
Cuts at: 1203 3313 6211 7188 1203
Size: 2110 2898 977 1327
Fragments arranged by size:
2898 2110 1327 977
AgeI A'CCGG_T
Cuts at: 2277 6143 2277
Size: 3866 3446
AhdI GACnn_n'nnGTC
Cuts at: 3702 6041 3702
Size: 2339 4973
AleI CACnn'nnGTG
Cuts at: 1166 2558 3224 1166
Size: 1392 666 5254
Fragments arranged by size:
5254 1392 666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4025 1184
Size: 2841 4471
AlwNI CAG_nnn'CTG
Cuts at: 3334 3517 3553 3922 3994 4039 4078 5055 6627
Size: 183 36 369 72 45 39 977 1572
Cuts at: 6627 3334
Size: 4019
Fragments arranged by size:
4019 1572 977 369 183 72 45 39
36
ApaI G_GGCC'C
Cuts at: 2543 3251 2543
Size: 708 6604
ApaLI G'TGCA_C
Cuts at: 5369 6525 5369
Size: 1156 6156
ApoI r'AATT_y
Cuts at: 1493 2389 4621 4847 4999 7108 1493
Size: 896 2232 226 152 2109 1697
Fragments arranged by size:
2232 2109 1697 896 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5866 160
Size: 872 4834 1606
Fragments arranged by size:
4834 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7312
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2295 2534 3050 3076 3195 4065
Size: 73 307 45 239 516 26 119 870
Cuts at: 4065 4130 4232 4637 1870
Size: 65 102 405 4545
Fragments arranged by size:
4545 870 516 405 307 239 119 102
73 65 45 26
BamHI G'GATC_C
Cuts at: 4303 4642 5009 4303
Size: 339 367 6606
Fragments arranged by size:
6606 367 339
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 2708 3427 3580 4633
Size: 325 217 627 487 434 719 153 1053
Cuts at: 4633 6088 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1053 719 627 487 434 325
217 153
BanII G_rGCy'C
Cuts at: 1391 2543 3101 3251 4100 4233 4631 1391
Size: 1152 558 150 849 133 398 4072
Fragments arranged by size:
4072 1152 849 558 398 150 133
BbeI G_GCGC'C
Cuts at: 2712 2712
Size: 7312
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2435 3778 928
Size: 971 536 1343 4462
Fragments arranged by size:
4462 1343 971 536
BbvCI CC'TCA_GC
Cuts at: 2504 2917 3035 3733 2504
Size: 413 118 698 6083
Fragments arranged by size:
6083 698 413 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3276 3310 5503 5537 1903
Size: 34 1339 34 2193 34 3678
Fragments arranged by size:
3678 2193 1339 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5204 6414 5204
Size: 1210 6102
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7312
BfrBI ATG'CAT
Cuts at: 4409 4409
Size: 7312
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3255 3594 5923
Size: 107 122 71 631 605 1583 339 2329
Cuts at: 5923 136
Size: 1525
Fragments arranged by size:
2329 1583 1525 631 605 339 122 107
71
BglII A'GATC_T
Cuts at: 7307 7307
Size: 7312
BlpI GC'TnA_GC
Cuts at: 4729 4729
Size: 7312
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2543 2555 3251 3342 3585 3945 5373
Size: 362 391 12 696 91 243 360 1428
Cuts at: 5373 6529 1790
Size: 1156 2573
Fragments arranged by size:
2573 1428 1156 696 391 362 360 243
91 12
BmgBI CAC'GTC
Cuts at: 1318 2556 1318
Size: 1238 6074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2649 2671 3322
Size: 416 53 83 399 24 1232 22 651
Cuts at: 3322 3382 3598 5043 6001 442
Size: 60 216 1445 958 1753
Fragments arranged by size:
1753 1445 1232 958 651 416 399 216
83 60 53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4091
Size: 7312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2420 2441 2724 2780 3328 3671 3802
Size: 1166 15 21 283 56 548 343 131
Cuts at: 3802 4510 5972 1239
Size: 708 1462 2579
Fragments arranged by size:
2579 1462 1166 708 548 343 283 131
56 21 15
Bpu10I CC'TnA_GC
Cuts at: 2504 2917 3035 3733 2504
Size: 413 118 698 6083
Fragments arranged by size:
6083 698 413 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5437 6302 6600 5437
Size: 865 298 6149
Fragments arranged by size:
6149 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3131 4083 5975 882
Size: 617 1632 952 1892 2219
Fragments arranged by size:
2219 1892 1632 952 617
BsaAI yAC'GTr
Cuts at: 493 1204 5103 493
Size: 711 3899 2702
Fragments arranged by size:
3899 2702 711
BsaBI GATnn'nnATC
Cuts at: 7306 7306
Size: 7312
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2709 3575 4422
Size: 53 83 186 936 534 642 866 847
Cuts at: 4422 5119 5501 6136 275
Size: 697 382 635 1451
Fragments arranged by size:
1451 936 866 847 697 642 635 534
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2277 3221 4402 4587 5745 6143 6417 6564 6975
Size: 944 1181 185 1158 398 274 147 411
Cuts at: 6975 2277
Size: 2614
Fragments arranged by size:
2614 1181 1158 944 411 398 274 185
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4082 4112 4307 4337 4492
Size: 30 165 30 2679 30 195 30 155
Cuts at: 4492 4522 1178
Size: 30 3968
Fragments arranged by size:
3968 2679 195 165 155 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 2905 3148 3512 3942 1186
Size: 1420 299 243 364 430 4556
Fragments arranged by size:
4556 1420 430 364 299 243
BseYI C'CCAG_C
Cuts at: 2015 2124 3500 3836 3994 4039 4266 4548 6515
Size: 109 1376 336 158 45 227 282 1967
Cuts at: 6515 7011 2015
Size: 496 2316
Fragments arranged by size:
2316 1967 1376 496 336 282 227 158
109 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2110 2570 2620 3530 3539 4110 1163
Size: 573 374 460 50 910 9 571 4365
Fragments arranged by size:
4365 910 573 571 460 374 50 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4762 5523 6551 1540
Size: 275 186 2761 761 1028 2301
Fragments arranged by size:
2761 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3759 4050 5373 5458 6529 3759
Size: 291 1323 85 1071 4542
Fragments arranged by size:
4542 1323 1071 291 85
BsmI GAATG_Cn'
Cuts at: 4823 4916 4823
Size: 93 7219
BsmBI CGTCTCn'nnnn_
Cuts at: 2094 2343 2094
Size: 249 7063
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2543 2555 3101 3251 3342 3585
Size: 399 362 391 12 546 150 91 243
Cuts at: 3585 3759 3945 4050 4100 4233 4631 5373 5458
Size: 174 186 105 50 133 398 742 85
Cuts at: 5458 6529 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 398 391 362
243 186 174 150 133 105 91 85
50 12
BspEI T'CCGG_A
Cuts at: 4402 4587 6975 4402
Size: 185 2388 4739
Fragments arranged by size:
4739 2388 185
BspHI T'CATG_A
Cuts at: 5201 6931 5201
Size: 1730 5582
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2265 2891 2995 3054 4357 4667 844
Size: 1334 87 626 104 59 1303 310 3489
Fragments arranged by size:
3489 1334 1303 626 310 104 87 59
BsrBI CCG'CTC
Cuts at: 1394 1823 2408 2937 3075 3466 5199 1394
Size: 429 585 529 138 391 1733 3507
Fragments arranged by size:
3507 1733 585 529 429 391 138
BsrDI GCAATG_nn'
Cuts at: 65 1248 3006 5807 5981 65
Size: 1183 1758 2801 174 1396
Fragments arranged by size:
2801 1758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2182 2277 2328 3905 5956 6143
Size: 110 235 137 95 51 1577 2051 187
Cuts at: 6143 1700
Size: 2869
Fragments arranged by size:
2869 2051 1577 235 187 137 110 95
51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7312
BssHII G'CGCG_C
Cuts at: 1925 2646 1925
Size: 721 6591
BssSI C'ACGA_G
Cuts at: 2588 3143 5372 6384 2588
Size: 555 2229 1012 3516
Fragments arranged by size:
3516 2229 1012 555
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3001 3517 3553 4078 794
Size: 2207 516 36 525 4028
Fragments arranged by size:
4028 2207 525 516 36
BstBI TT'CG_AA
Cuts at: 6116 6116
Size: 7312
BstEII G'GTnAC_C
Cuts at: 1184 3212 1184
Size: 2028 5284
BstXI CCAn_nnnn'nTGG
Cuts at: 2771 3706 2771
Size: 935 6377
BstYI r'GATC_y
Cuts at: 1972 3660 4303 4642 5009 5394 5411 6863 6949
Size: 1688 643 339 367 385 17 1452 86
Cuts at: 6949 6961 7307 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1688 1452 643 385 367 346 339
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3102 3102
Size: 7312
BtgI C'CryG_G
Cuts at: 513 1065 2004 2266 3021 3207 3448 4059 513
Size: 552 939 262 755 186 241 611 3766
Fragments arranged by size:
3766 939 755 611 552 262 241 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6573
Fragments arranged by size:
6573 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 3183 3900 4828 5622 5642 2150
Size: 1033 717 928 794 20 3820
Fragments arranged by size:
3820 1033 928 794 717 20
ClaI AT'CG_AT
Cuts at: 1554 2223 1554
Size: 669 6643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2988 3023 529
Size: 35 2424 35 4818
Fragments arranged by size:
4818 2424 35 35
DraI TTT'AAA
Cuts at: 4616 4616
Size: 7312
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4270 6319 809
Size: 507 2954 2049 1802
Fragments arranged by size:
2954 2049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2928 3060 4294
Size: 54 1425 50 275 18 1098 132 1234
Cuts at: 4294 4343 4461 4759 5648 7277 8
Size: 49 118 298 889 1629 43
Fragments arranged by size:
1629 1425 1234 1098 889 298 275 132
118 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4759 1537
Size: 275 2947 4090
Fragments arranged by size:
4090 2947 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3166 3375 3615 3764 4017 5242 1353
Size: 1813 209 240 149 253 1225 3423
Fragments arranged by size:
3423 1813 1225 253 240 209 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2015 3480 3791 5899 6273 6419
Size: 1064 138 426 1465 311 2108 374 146
Cuts at: 6419 387
Size: 1280
Fragments arranged by size:
2108 1465 1280 1064 426 374 311 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2420 2441 2724 2780 3328 3458
Size: 393 773 15 21 283 56 548 130
Cuts at: 3458 3671 3802 3944 4510 5375 5972 6759 7027
Size: 213 131 142 566 865 597 787 268
Cuts at: 7027 1239
Size: 1524
Fragments arranged by size:
1524 865 787 773 597 566 548 393
283 268 213 142 131 130 56 21
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2703 2993 2703
Size: 290 7022
EcoO109I rG'GnC_Cy
Cuts at: 2167 2233 2254 2461 2539 2540 2801 2848 3130
Size: 66 21 207 78 1 261 47 282
Cuts at: 3130 3247 3596 3928 4221 2167
Size: 117 349 332 293 5258
Fragments arranged by size:
5258 349 332 293 282 261 207 117
78 66 47 21 1
EcoRI G'AATT_C
Cuts at: 4621 4999 4621
Size: 378 6934
FspI TGC'GCA
Cuts at: 3189 5818 3189
Size: 2629 4683
HaeII r_GCGC'y
Cuts at: 1678 2521 2712 3418 3685 3740 4165 4261 6459
Size: 843 191 706 267 55 425 96 2198
Cuts at: 6459 1678
Size: 2531
Fragments arranged by size:
2531 2198 843 706 425 267 191 96
55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3688 3720 4082 4114 4307
Size: 32 135 32 2116 32 362 32 193
Cuts at: 4307 4339 5953 5985 6027 6059 1373
Size: 32 1614 32 42 32 2626
Fragments arranged by size:
2626 2116 1614 362 193 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4398 4656 4902 669
Size: 988 281 2460 258 246 3079
Fragments arranged by size:
3079 2460 988 281 258 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7312
HpaI GTT'AAC
Cuts at: 4902 4902
Size: 7312
KasI G'GCGC_C
Cuts at: 2708 2708
Size: 7312
KpnI G_GTAC'C
Cuts at: 2278 4637 2278
Size: 2359 4953
MfeI C'AATT_G
Cuts at: 4911 4911
Size: 7312
MluI A'CGCG_T
Cuts at: 7188 7188
Size: 7312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6426 6610 1305
Size: 5121 184 2007
Fragments arranged by size:
5121 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3062 4296 4345 4463 10
Size: 54 1425 343 1230 1234 49 118 2859
Fragments arranged by size:
2859 1425 1234 1230 343 118 54 49
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 3224 5270 5629 5788 518
Size: 648 1392 666 2046 359 159 2042
Fragments arranged by size:
2046 2042 1392 666 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2268 2617 2689 3504 3544 3550 4036
Size: 488 174 349 72 815 40 6 486
Cuts at: 4036 4270 4438 4728 5405 6553 6798 1606
Size: 234 168 290 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 677 488 486 349 290
245 234 174 168 72 40 6
NaeI GCC'GGC
Cuts at: 2184 3907 2184
Size: 1723 5589
NarI GG'CG_CC
Cuts at: 2709 2709
Size: 7312
NcoI C'CATG_G
Cuts at: 513 1065 2004 3448 4059 513
Size: 552 939 1444 611 3766
Fragments arranged by size:
3766 1444 939 611 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7312
NgoMIV G'CCGG_C
Cuts at: 2182 3905 2182
Size: 1723 5589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7312
NotI GC'GGCC_GC
Cuts at: 4759 4759
Size: 7312
NsiI A_TGCA'T
Cuts at: 4411 4411
Size: 7312
NspI r_CATG'y
Cuts at: 1132 1229 2159 2826 4413 4670 6215 1132
Size: 97 930 667 1587 257 1545 2229
Fragments arranged by size:
2229 1587 1545 930 667 257 97
PasI CC'CwG_GG
Cuts at: 1348 2192 2229 2368 2481 2493 4417 1348
Size: 844 37 139 113 12 1924 4243
Fragments arranged by size:
4243 1924 844 139 113 37 12
PciI A'CATG_T
Cuts at: 6211 6211
Size: 7312
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3334 3994 1108
Size: 2226 660 4426
Fragments arranged by size:
4426 2226 660
PfoI T'CCnGG_A
Cuts at: 2457 2662 3150 3617 3884 2457
Size: 205 488 467 267 5885
Fragments arranged by size:
5885 488 467 267 205
PmeI GTTT'AAAC
Cuts at: 4616 4616
Size: 7312
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5381 5413 6894 6926 5381
Size: 32 1481 32 5767
Fragments arranged by size:
5767 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2167 2233 2254 2461 2801 3130 4221 2167
Size: 66 21 207 340 329 1091 5258
Fragments arranged by size:
5258 1091 340 329 207 66 21
PshAI GACnn'nnGTC
Cuts at: 1613 2435 1613
Size: 822 6490
PsiI TTA'TAA
Cuts at: 4882 4882
Size: 7312
PspOMI G'GGCC_C
Cuts at: 2539 3247 2539
Size: 708 6604
PstI C_TGCA'G
Cuts at: 830 2997 3071 3549 3678 3914 4086 4664 830
Size: 2167 74 478 129 236 172 578 3478
Fragments arranged by size:
3478 2167 578 478 236 172 129 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7312
PvuII CAG'CTG
Cuts at: 2094 2617 2689 3504 3544 3550 4270 2094
Size: 523 72 815 40 6 720 5136
Fragments arranged by size:
5136 815 720 523 72 40 6
SacII CC_GC'GG
Cuts at: 2269 2269
Size: 7312
SalI G'TCGA_C
Cuts at: 1936 4654 1936
Size: 2718 4594
SanDI GG'GwC_CC
Cuts at: 2233 3130 2233
Size: 897 6415
SapI GCTCTTCn'nnn_
Cuts at: 3764 3764
Size: 7312
SbfI CC_TGCA'GG
Cuts at: 4664 4664
Size: 7312
ScaI AGT'ACT
Cuts at: 5560 7272 5560
Size: 1712 5600
SfcI C'TryA_G
Cuts at: 826 1045 2993 3067 3124 3545 3674 3910 4082
Size: 219 1948 74 57 421 129 236 172
Cuts at: 4082 4660 5795 6476 6667 826
Size: 578 1135 681 191 1471
Fragments arranged by size:
1948 1471 1135 681 578 421 236 219
191 172 129 74 57
SfoI GGC'GCC
Cuts at: 2710 2710
Size: 7312
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7312
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7312
SmaI CCC'GGG
Cuts at: 1872 2252 3052 4234 4639 1872
Size: 380 800 1182 405 4545
Fragments arranged by size:
4545 1182 800 405 380
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5416 6317 6579 820
Size: 197 926 3473 901 262 1553
Fragments arranged by size:
3473 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5103 493
Size: 4610 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7312
SphI G_CATG'C
Cuts at: 1132 4413 4670 1132
Size: 3281 257 3774
Fragments arranged by size:
3774 3281 257
SspI AAT'ATT
Cuts at: 5 52 5236 5
Size: 47 5184 2081
Fragments arranged by size:
5184 2081 47
StuI AGG'CCT
Cuts at: 1517 2633 3750 4140 4523 1517
Size: 1116 1117 390 383 4306
Fragments arranged by size:
4306 1117 1116 390 383
StyI C'CwwG_G
Cuts at: 513 1065 2004 2053 2163 2770 3448 4059 4751
Size: 552 939 49 110 607 678 611 692
Cuts at: 4751 513
Size: 3074
Fragments arranged by size:
3074 939 692 678 611 607 552 110
49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3200 3869 3985 3995 4031 5339 5356
Size: 1255 1620 669 116 10 36 1308 17
Cuts at: 5356 5509 5694 6139 325
Size: 153 185 445 1498
Fragments arranged by size:
1620 1498 1308 1255 669 445 185 153
116 36 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5558 7270 96
Size: 274 80 33 51 1443 3581 1712 138
Fragments arranged by size:
3581 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 4492 4524 7272 7304 2159
Size: 32 2301 32 2748 32 2167
Fragments arranged by size:
2748 2301 2167 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7312
XbaI T'CTAG_A
Cuts at: 4648 4648
Size: 7312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2465 3748 2465
Size: 1283 6029
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7312
XmaI C'CCGG_G
Cuts at: 1870 2250 3050 4232 4637 1870
Size: 380 800 1182 405 4545
Fragments arranged by size:
4545 1182 800 405 380
XmnI GAAnn'nnTTC
Cuts at: 5441 5441
Size: 7312
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2068 5120 276
Size: 53 83 186 936 534 3052 2468
Fragments arranged by size:
3052 2468 936 534 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NruI PacI PmlI
PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI