Restriction map for
pFN21AB0435
(Circular) (Six-base) MAPSORT of: pFN21AB0435.seq Check: 6581 from: 1 to: 6049
pFN21AB0435 6049 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3331 3331
Size: 6049
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2856 3392 1937
Size: 919 536 4594
Fragments arranged by size:
4594 919 536
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2445 2657 3173 4112 5496 5764 1632
Size: 813 212 516 939 1384 268 1917
Fragments arranged by size:
1917 1384 939 813 516 268 212
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 3009 4948 5925 1203
Size: 1806 1939 977 1327
Fragments arranged by size:
1939 1806 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AlwNI CAG_nnn'CTG
Cuts at: 2842 3792 5364 2842
Size: 950 1572 3527
Fragments arranged by size:
3527 1572 950
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 2765 3358 3584 3736 5845 1493
Size: 1272 593 226 152 2109 1697
Fragments arranged by size:
2109 1697 1272 593 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2491 2677 3062 3374 1870
Size: 73 133 415 186 385 312 4545
Fragments arranged by size:
4545 415 385 312 186 133 73
BamHI G'GATC_C
Cuts at: 3379 3746 3379
Size: 367 5682
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2425 2486 3126 3370
Size: 325 217 627 405 233 61 640 244
Cuts at: 3370 4825 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 640 627 405 325 244 233
217 61
BanII G_rGCy'C
Cuts at: 1391 2172 2874 2894 3368 1391
Size: 781 702 20 474 4072
Fragments arranged by size:
4072 781 702 474 20
BbeI G_GCGC'C
Cuts at: 3130 3130
Size: 6049
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2645 3074 928
Size: 971 746 429 3903
Fragments arranged by size:
3903 971 746 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2798 2832 2984 3018 4240 4274 1903
Size: 34 861 34 152 34 1222 34 3678
Fragments arranged by size:
3678 1222 861 152 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 2568 1548
Size: 1020 5029
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2358 4660 136
Size: 107 122 71 631 605 686 2302 1525
Fragments arranged by size:
2302 1525 686 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 2071 2843 3083 3466 2071
Size: 772 240 383 4654
Fragments arranged by size:
4654 772 383 240
Bme1580I G_kGCm'C
Cuts at: 1790 2147 4110 5266 1790
Size: 357 1963 1156 2573
Fragments arranged by size:
2573 1963 1156 357
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3780 4738 442
Size: 416 53 83 399 24 2363 958 1753
Fragments arranged by size:
2363 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 2772 3320 4709 1239
Size: 1154 379 548 1389 2579
Fragments arranged by size:
2579 1389 1154 548 379
Bpu10I CC'TnA_GC
Cuts at: 3231 3231
Size: 6049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4174 5039 5337 4174
Size: 865 298 4886
Fragments arranged by size:
4886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4712 882
Size: 617 3213 2219
Fragments arranged by size:
3213 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 3840 493
Size: 711 1889 747 2702
Fragments arranged by size:
2702 1889 747 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2033 2269 3127 3856
Size: 53 83 186 936 500 236 858 729
Cuts at: 3856 4238 4873 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 858 729 635 500 382 236
186 83 53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2494 2524 2770 2800 3269
Size: 30 165 30 1091 30 246 30 469
Cuts at: 3269 3299 1178
Size: 30 3928
Fragments arranged by size:
3928 1091 469 246 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2729 2882 1186
Size: 994 549 153 4353
Fragments arranged by size:
4353 994 549 153
BseYI C'CCAG_C
Cuts at: 2656 3330 5252 5748 2656
Size: 674 1922 496 2957
Fragments arranged by size:
2957 1922 674 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2008 3272 3309 1163
Size: 573 272 1264 37 3903
Fragments arranged by size:
3903 1264 573 272 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 4110 4195 5266 2072
Size: 2038 85 1071 2855
Fragments arranged by size:
2855 2038 1071 85
BsmI GAATG_Cn'
Cuts at: 3084 3560 3653 3084
Size: 476 93 5480
Fragments arranged by size:
5480 476 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2147 2172 2874 2894 3368 4110
Size: 399 282 75 25 702 20 474 742
Cuts at: 4110 4195 5266 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 474 399 282 85
75 25 20
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3331 3404 844
Size: 2487 73 3489
Fragments arranged by size:
3489 2487 73
BsrBI CCG'CTC
Cuts at: 1394 1823 2081 3262 3936 1394
Size: 429 258 1181 674 3507
Fragments arranged by size:
3507 1181 674 429 258
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 2583 4544 4718 65
Size: 1183 1323 12 1961 174 1396
Fragments arranged by size:
1961 1396 1323 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2406 794
Size: 1612 4437
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2988 3379 3746 4131 4148 5600 5686 5698
Size: 1016 391 367 385 17 1452 86 12
Cuts at: 5698 6044 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1016 391 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3039 513
Size: 552 939 1035 3523
Fragments arranged by size:
3523 1035 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2587 487
Size: 683 56 1361 3949
Fragments arranged by size:
3949 1361 683 56
BtsI GCAGTG_nn'
Cuts at: 2559 3565 4359 4379 2559
Size: 1006 794 20 4229
Fragments arranged by size:
4229 1006 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2995 3030 529
Size: 35 2431 35 3548
Fragments arranged by size:
3548 2431 35 35
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2556 2598 2928
Size: 54 1425 50 275 18 726 42 330
Cuts at: 2928 3496 4385 6014 8
Size: 568 889 1629 43
Fragments arranged by size:
1629 1425 889 726 568 330 275 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 3149 3979 1353
Size: 1325 471 830 3423
Fragments arranged by size:
3423 1325 830 471
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2246 4636 5010 5156 387
Size: 1064 138 657 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 657 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 2445 2657 2772 3173 3320 4112
Size: 393 761 52 212 115 401 147 792
Cuts at: 4112 4709 5496 5764 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 792 787 761 597 401 393 268
212 147 115 52
EcoO109I rG'GnC_Cy
Cuts at: 2728 2728
Size: 6049
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 3130 5196 1678
Size: 1452 2066 2531
Fragments arranged by size:
2531 2066 1452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2770 2802 2807 2839 4690
Size: 32 135 32 1198 32 5 32 1851
Cuts at: 4690 4722 4764 4796 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1851 1198 135 42 32 32 32
32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3393 3639 669
Size: 988 281 919 536 246 3079
Fragments arranged by size:
3079 988 919 536 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KasI G'GCGC_C
Cuts at: 3126 3126
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5163 5347 1305
Size: 3858 184 2007
Fragments arranged by size:
3858 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 2600 2930 10
Size: 54 1425 343 726 42 330 3129
Fragments arranged by size:
3129 1425 726 343 330 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 2621 4007 4366 4525 518
Size: 648 1410 45 1386 359 159 2042
Fragments arranged by size:
2042 1410 1386 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2839 3465 4142 5290 5535 1606
Size: 1233 626 677 1148 245 2120
Fragments arranged by size:
2120 1233 1148 677 626 245
NarI GG'CG_CC
Cuts at: 3127 3127
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4558
Fragments arranged by size:
4558 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2371 3190 3341 3407 4952 1132
Size: 97 1142 819 151 66 1545 2229
Fragments arranged by size:
2229 1545 1142 819 151 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6049
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 4118 4150 5631 5663 2804
Size: 32 1282 32 1481 32 3190
Fragments arranged by size:
3190 1481 1282 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2096 2128 2096
Size: 32 6017
PstI C_TGCA'G
Cuts at: 830 3401 830
Size: 2571 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2839 2839
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 2855 3391 1936
Size: 919 536 4594
Fragments arranged by size:
4594 919 536
SapI GCTCTTCn'nnn_
Cuts at: 2678 2678
Size: 6049
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 2111 4297 6009 2111
Size: 2186 1712 2151
Fragments arranged by size:
2186 2151 1712
SfcI C'TryA_G
Cuts at: 826 1045 3220 3397 4532 5213 5404 826
Size: 219 2175 177 1135 681 191 1471
Fragments arranged by size:
2175 1471 1135 681 219 191 177
SfoI GGC'GCC
Cuts at: 3128 3128
Size: 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 2679 3376 1872
Size: 807 697 4545
Fragments arranged by size:
4545 807 697
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3062 4153 5054 5316 820
Size: 197 926 1119 1091 901 262 1553
Fragments arranged by size:
1553 1119 1091 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3190 3407 1132
Size: 2058 217 3774
Fragments arranged by size:
3774 2058 217
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2004 2439 2545 3488 513
Size: 552 939 435 106 943 3074
Fragments arranged by size:
3074 943 939 552 435 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2398 4076 4093 4246 4431 4876 325
Size: 1255 818 1678 17 153 185 445 1498
Fragments arranged by size:
1678 1498 1255 818 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 4295 6007
Size: 274 80 33 51 1443 132 2186 1712
Cuts at: 6007 96
Size: 138
Fragments arranged by size:
2186 1712 1443 274 138 132 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2494 2526 3269 3301 6009 6041 2494
Size: 32 743 32 2708 32 2502
Fragments arranged by size:
2708 2502 743 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2446 2593 2011
Size: 435 147 5467
Fragments arranged by size:
5467 435 147
XhoI C'TCGA_G
Cuts at: 1943 3062 1943
Size: 1119 4930
XmaI C'CCGG_G
Cuts at: 1870 2677 3374 1870
Size: 807 697 4545
Fragments arranged by size:
4545 807 697
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI