Restriction map for
pFN21AB0431
(Circular) (Six-base) MAPSORT of: pFN21AB0431.seq Check: 5328 from: 1 to: 6133
pFN21AB0431 6133 bp
With 182 enzymes: SgfI *
January 27, 2011 14:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3014 3943 278
Size: 53 83 186 936 1478 929 2468
Fragments arranged by size:
2468 1478 936 929 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 2746 4260 4633 1563
Size: 1183 1514 373 3063
Fragments arranged by size:
3063 1514 1183 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3093 4196 5580 5848 1632
Size: 1461 1103 1384 268 1917
Fragments arranged by size:
1917 1461 1384 1103 268
AflII C'TTAA_G
Cuts at: 820 1017 2855 820
Size: 197 1838 4098
Fragments arranged by size:
4098 1838 197
AflIII A'CryG_T
Cuts at: 1203 5032 6009 1203
Size: 3829 977 1327
Fragments arranged by size:
3829 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 3876 5448 3876
Size: 1572 4561
ApaI G_GGCC'C
Cuts at: 2063 2063
Size: 6133
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 2694 3442 3668 3820 5929 1493
Size: 1201 748 226 152 2109 1697
Fragments arranged by size:
2109 1697 1201 748 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2352 4687 160
Size: 872 1320 2335 1606
Fragments arranged by size:
2335 1606 1320 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 3458 1870
Size: 73 119 1396 4545
Fragments arranged by size:
4545 1396 119 73
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3454 4909 618
Size: 325 217 627 1667 1455 1842
Fragments arranged by size:
1842 1667 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2063 2631 3003 3118 3223 3343 3452 1391
Size: 672 568 372 115 105 120 109 4072
Fragments arranged by size:
4072 672 568 372 120 115 109 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2579 2624 928
Size: 971 680 45 4437
Fragments arranged by size:
4437 971 680 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2678 2712 3378 3412 4324
Size: 34 50 34 657 34 666 34 912
Cuts at: 4324 4358 1903
Size: 34 3678
Fragments arranged by size:
3678 912 666 657 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3133 4744 136
Size: 107 122 71 631 605 1461 1611 1525
Fragments arranged by size:
1611 1525 1461 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3053 6128 3053
Size: 3075 3058
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2953 4194 5350 1790
Size: 273 890 1241 1156 2573
Fragments arranged by size:
2573 1241 1156 890 273
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3254 3864 4822
Size: 416 53 83 399 24 1837 610 958
Cuts at: 4822 442
Size: 1753
Fragments arranged by size:
1837 1753 958 610 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2946 3014 4793 1239
Size: 1037 670 68 1779 2579
Fragments arranged by size:
2579 1779 1037 670 68
Bpu10I CC'TnA_GC
Cuts at: 2204 2204
Size: 6133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 3042 4258 5123 5421 2323
Size: 719 1216 865 298 3035
Fragments arranged by size:
3035 1216 865 719 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3924 493
Size: 711 2720 2702
Fragments arranged by size:
2720 2702 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2135 3011 3940 4322
Size: 53 83 186 936 602 876 929 382
Cuts at: 4322 4957 275
Size: 635 1451
Fragments arranged by size:
1451 936 929 876 635 602 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2153 4566 4964 5238 5385 5796 2153
Size: 2413 398 274 147 411 2490
Fragments arranged by size:
2490 2413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 1178
Size: 30 165 30 663 30 5215
Fragments arranged by size:
5215 663 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2273 2737 2740 2922 2927 3011 1186
Size: 1015 72 464 3 182 5 84 4308
Fragments arranged by size:
4308 1015 464 182 84 72 5 3
BseYI C'CCAG_C
Cuts at: 2599 5336 5832 2599
Size: 2737 496 2900
Fragments arranged by size:
2900 2737 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 2979 1163
Size: 573 381 862 4317
Fragments arranged by size:
4317 862 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 3583 4344 5372 1540
Size: 275 186 45 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 2631 3039 3118 3343 4194 4279 5350 2631
Size: 408 79 225 851 85 1071 3414
Fragments arranged by size:
3414 1071 851 408 225 85 79
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
BsmBI CGTCTCn'nnnn_
Cuts at: 2126 3002 2126
Size: 876 5257
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2631 2953 3003 3039 3118 3223
Size: 399 273 568 322 50 36 79 105
Cuts at: 3223 3343 3452 4194 4279 5350 1391
Size: 120 109 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 568 399 322 273 120
109 105 85 79 50 36
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3488 844
Size: 2644 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2067 4020 1394
Size: 429 244 1953 3507
Fragments arranged by size:
3507 1953 429 244
BsrDI GCAATG_nn'
Cuts at: 65 1248 2631 4628 4802 65
Size: 1183 1383 1997 174 1396
Fragments arranged by size:
1997 1396 1383 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3309 4777 4964 1700
Size: 110 1499 1468 187 2869
Fragments arranged by size:
2869 1499 1468 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 3122 4193 5205 3122
Size: 1071 1012 4050
Fragments arranged by size:
4050 1071 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstXI CCAn_nnnn'nTGG
Cuts at: 2980 2980
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 3053 3378 3463 3830 4215 4232 5684 5770
Size: 1081 325 85 367 385 17 1452 86
Cuts at: 5770 5782 6128 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1081 385 367 346 325 86
85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2979 3234 513
Size: 552 1914 255 3412
Fragments arranged by size:
3412 1914 552 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2448 3031 3649 4443 4463 2448
Size: 583 618 794 20 4118
Fragments arranged by size:
4118 794 618 583 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 3181 3311
Size: 54 1425 50 275 18 213 1138 130
Cuts at: 3311 3580 4469 6098 8
Size: 269 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 275 269 213 130
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 3580 1537
Size: 275 231 1537 4090
Fragments arranged by size:
4090 1537 275 231
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2562 2697 2751 2770 3244 3410 3417
Size: 833 376 135 54 19 474 166 7
Cuts at: 3417 4063 1353
Size: 646 3423
Fragments arranged by size:
3423 833 646 474 376 166 135 54
19 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2210 3381 4720 5094 5240 387
Size: 1064 138 621 1171 1339 374 146 1280
Fragments arranged by size:
1339 1280 1171 1064 621 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 3116 3341 2629
Size: 487 225 5421
Fragments arranged by size:
5421 487 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2946 3014 3093 4196 4793 5580
Size: 393 644 670 68 79 1103 597 787
Cuts at: 5580 5848 1239
Size: 268 1524
Fragments arranged by size:
1524 1103 787 670 644 597 393 268
79 68
EcoNI CCTnn'n_nnAGG
Cuts at: 2207 2243 2207
Size: 36 6097
EcoO109I rG'GnC_Cy
Cuts at: 2059 3176 2059
Size: 1117 5016
EcoRI G'AATT_C
Cuts at: 2694 3442 3820 2694
Size: 748 378 5007
Fragments arranged by size:
5007 748 378
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 5280 1678
Size: 3602 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2308 2340 4774
Size: 32 135 32 494 32 210 32 2434
Cuts at: 4774 4806 4848 4880 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2434 494 210 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3723 669
Size: 988 281 1539 246 3079
Fragments arranged by size:
3079 1539 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2849 748
Size: 2101 4032
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5247 5431 1305
Size: 3942 184 2007
Fragments arranged by size:
3942 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3183 10
Size: 54 1425 343 1351 2960
Fragments arranged by size:
2960 1425 1351 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3042 3093 4091 4450 4609 518
Size: 648 1876 51 998 359 159 2042
Fragments arranged by size:
2042 1876 998 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2254 2266 2275 3021 3216 3325 3549 4226
Size: 648 12 9 746 195 109 224 677
Cuts at: 4226 5374 5619 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 746 677 648 245 224 195
109 12 9
NcoI C'CATG_G
Cuts at: 513 1065 2979 3234 513
Size: 552 1914 255 3412
Fragments arranged by size:
3412 1914 552 255
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NruI TCG'CGA
Cuts at: 2184 2184
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 3491 5036 1132
Size: 97 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PfoI T'CCnGG_A
Cuts at: 3334 3334
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2667 4202 4234 5715 5747 2635
Size: 32 1535 32 1481 32 3021
Fragments arranged by size:
3021 1535 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3176 3176
Size: 6133
PshAI GACnn'nnGTC
Cuts at: 1613 2316 3270 1613
Size: 703 954 4476
Fragments arranged by size:
4476 954 703
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PspOMI G'GGCC_C
Cuts at: 2059 2059
Size: 6133
PstI C_TGCA'G
Cuts at: 830 2443 3485 830
Size: 1613 1042 3478
Fragments arranged by size:
3478 1613 1042
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2254 2266 3325 2254
Size: 12 1059 5062
Fragments arranged by size:
5062 1059 12
SacI G_AGCT'C
Cuts at: 2631 3118 3343 2631
Size: 487 225 5421
Fragments arranged by size:
5421 487 225
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SapI GCTCTTCn'nnn_
Cuts at: 3244 3244
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SfcI C'TryA_G
Cuts at: 826 1045 2439 3481 4616 5297 5488 826
Size: 219 1394 1042 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 1042 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 2855 3057 4237 5138 5400
Size: 197 926 395 517 202 1180 901 262
Cuts at: 5400 820
Size: 1553
Fragments arranged by size:
1553 1180 926 901 517 395 262 202
197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 2034 2427 1517
Size: 517 393 5223
Fragments arranged by size:
5223 517 393
StyI C'CwwG_G
Cuts at: 513 1065 2244 2979 3234 3572 513
Size: 552 1179 735 255 338 3074
Fragments arranged by size:
3074 1179 735 552 338 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3295 4160 4177 4330 4515 4960 325
Size: 1255 1715 865 17 153 185 445 1498
Fragments arranged by size:
1715 1498 1255 865 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4379 6091 96
Size: 274 80 33 51 1443 2402 1712 138
Fragments arranged by size:
2402 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6093 6125 6093
Size: 32 6101
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2653 3241 2251
Size: 402 588 5143
Fragments arranged by size:
5143 588 402
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3012 3941 276
Size: 53 83 186 936 1478 929 2468
Fragments arranged by size:
2468 1478 936 929 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI