Restriction map for
pFN21AB0430
(Circular) (Six-base) MAPSORT of: pFN21AB0430.seq Check: 8593 from: 1 to: 7261
pFN21AB0430 7261 bp
With 182 enzymes: SgfI *
April 18, 2011 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2622 2622
Size: 7261
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5071 278
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3070 4604 1937
Size: 1133 1534 4594
Fragments arranged by size:
4594 1534 1133
Acc65I G'GTAC_C
Cuts at: 4222 4582 4222
Size: 360 6901
AclI AA'CG_TT
Cuts at: 1563 5388 5761 1563
Size: 3825 373 3063
Fragments arranged by size:
3825 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2956 2980 3162 5324 6708 6976 1632
Size: 1324 24 182 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 1324 268 182 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7064
AflIII A'CryG_T
Cuts at: 1203 4260 6160 7137 1203
Size: 3057 1900 977 1327
Fragments arranged by size:
3057 1900 1327 977
AgeI A'CCGG_T
Cuts at: 6092 6092
Size: 7261
AhdI GACnn_n'nnGTC
Cuts at: 5990 5990
Size: 7261
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4140 1184
Size: 2956 4305
AlwNI CAG_nnn'CTG
Cuts at: 2731 3689 3872 4153 4207 5004 6576 2731
Size: 958 183 281 54 797 1572 3416
Fragments arranged by size:
3416 1572 958 797 281 183 54
ApaI G_GGCC'C
Cuts at: 2226 3017 2226
Size: 791 6470
ApaLI G'TGCA_C
Cuts at: 4376 5318 6474 4376
Size: 942 1156 5163
Fragments arranged by size:
5163 1156 942
ApoI r'AATT_y
Cuts at: 1493 2326 3692 4570 4796 4948 7057 1493
Size: 833 1366 878 226 152 2109 1697
Fragments arranged by size:
2109 1697 1366 878 833 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5815 160
Size: 872 4783 1606
Fragments arranged by size:
4783 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 4498 4586 1870
Size: 73 155 2400 88 4545
Fragments arranged by size:
4545 2400 155 88 73
AvrII C'CTAG_G
Cuts at: 3503 3503
Size: 7261
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3940 3973 4206 4239 3940
Size: 33 233 33 6962
Fragments arranged by size:
6962 233 33 33
BamHI G'GATC_C
Cuts at: 4591 4958 4591
Size: 367 6894
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3022 3214 3259 4222 4541
Size: 325 217 627 1235 192 45 963 319
Cuts at: 4541 4582 6037 618
Size: 41 1455 1842
Fragments arranged by size:
1842 1455 1235 963 627 325 319 217
192 45 41
BanII G_rGCy'C
Cuts at: 1391 2226 2426 2964 3017 3231 4029 4176 4580
Size: 835 200 538 53 214 798 147 404
Cuts at: 4580 1391
Size: 4072
Fragments arranged by size:
4072 835 798 538 404 214 200 147
53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3150 4094 928
Size: 971 1251 944 4095
Fragments arranged by size:
4095 1251 971 944
BbvCI CC'TCA_GC
Cuts at: 3854 3914 3854
Size: 60 7201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5452 5486 1903
Size: 34 3515 34 3678
Fragments arranged by size:
3678 3515 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3652 4496 5153 6363 3652
Size: 844 657 1210 4550
Fragments arranged by size:
4550 1210 844 657
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7261
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2197 2719 3764
Size: 107 122 71 631 605 525 522 1045
Cuts at: 3764 4470 4526 5872 136
Size: 706 56 1346 1525
Fragments arranged by size:
1525 1346 1045 706 631 605 525 522
122 107 71 56
BglII A'GATC_T
Cuts at: 7256 7256
Size: 7261
BlpI GC'TnA_GC
Cuts at: 3609 4678 3609
Size: 1069 6192
Bme1580I G_kGCm'C
Cuts at: 1790 2226 3017 3027 4048 4380 4475 4544 5322
Size: 436 791 10 1021 332 95 69 778
Cuts at: 5322 6478 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1021 791 778 436 332 95
69 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7261
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4992 5950 442
Size: 416 53 83 399 24 3575 958 1753
Fragments arranged by size:
3575 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3823 3823
Size: 7261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2404 2519 2656 3091 3245 3647 3732 5921
Size: 1165 115 137 435 154 402 85 2189
Cuts at: 5921 1239
Size: 2579
Fragments arranged by size:
2579 2189 1165 435 402 154 137 115
85
Bpu10I CC'TnA_GC
Cuts at: 2616 3532 3854 3914 4512 2616
Size: 916 322 60 598 5365
Fragments arranged by size:
5365 916 598 322 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2302 2324 4043 5386 6251 6549 2302
Size: 22 1719 1343 865 298 3014
Fragments arranged by size:
3014 1719 1343 865 298 22
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5924 882
Size: 617 4425 2219
Fragments arranged by size:
4425 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5052 493
Size: 711 3848 2702
Fragments arranged by size:
3848 2702 711
BsaBI GATnn'nnATC
Cuts at: 7255 7255
Size: 7261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2864 5068 5450 6085
Size: 53 83 186 936 1331 2204 382 635
Cuts at: 6085 275
Size: 1451
Fragments arranged by size:
2204 1451 1331 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2475 3657 5694 6092 6366 6513 6924 2475
Size: 1182 2037 398 274 147 411 2812
Fragments arranged by size:
2812 2037 1182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2669 3310 3340 3842
Size: 30 165 30 1236 30 641 30 502
Cuts at: 3842 3872 1178
Size: 30 4567
Fragments arranged by size:
4567 1236 641 502 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 2723 3644 4154 4184 1186
Size: 1534 3 921 510 30 4263
Fragments arranged by size:
4263 1534 921 510 30 3
BseYI C'CCAG_C
Cuts at: 2060 2588 2692 2966 3392 3397 3814 3821 4003
Size: 528 104 274 426 5 417 7 182
Cuts at: 4003 4047 4052 4146 4468 6464 6960 2060
Size: 44 5 94 322 1996 496 2361
Fragments arranged by size:
2361 1996 528 496 426 417 322 274
182 104 94 44 7 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3685 4277 1163
Size: 573 1949 592 4147
Fragments arranged by size:
4147 1949 592 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4711 5472 6500 1540
Size: 275 186 2710 761 1028 2301
Fragments arranged by size:
2710 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4037 4176 4380 5322 5407 6478 4037
Size: 139 204 942 85 1071 4820
Fragments arranged by size:
4820 1071 942 204 139 85
BsiWI C'GTAC_G
Cuts at: 4286 4286
Size: 7261
BsmI GAATG_Cn'
Cuts at: 4772 4865 4772
Size: 93 7168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2426 2964 3017 3027 3231 4029
Size: 399 436 200 538 53 10 204 798
Cuts at: 4029 4037 4048 4176 4380 4475 4544 4580 5322
Size: 8 11 128 204 95 69 36 742
Cuts at: 5322 5407 6478 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 798 742 538 436 399 204
204 200 128 95 85 69 53 36
11 10 8
BspEI T'CCGG_A
Cuts at: 6924 6924
Size: 7261
BspHI T'CATG_A
Cuts at: 5150 6880 5150
Size: 1730 5531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2012 2032 2128 2622 4616 844
Size: 1168 20 96 494 1994 3489
Fragments arranged by size:
3489 1994 1168 494 96 20
BsrBI CCG'CTC
Cuts at: 1394 1823 2133 3802 4433 5148 1394
Size: 429 310 1669 631 715 3507
Fragments arranged by size:
3507 1669 715 631 429 310
BsrDI GCAATG_nn'
Cuts at: 65 1248 2657 2842 5756 5930 65
Size: 1183 1409 185 2914 174 1396
Fragments arranged by size:
2914 1409 1396 1183 185 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 3951 5905 6092 1700
Size: 110 199 1942 1954 187 2869
Fragments arranged by size:
2869 1954 1942 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7261
BssHII G'CGCG_C
Cuts at: 1925 2013 1925
Size: 88 7173
BssSI C'ACGA_G
Cuts at: 4028 5321 6333 4028
Size: 1293 1012 4956
Fragments arranged by size:
4956 1293 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4207 794
Size: 3413 3848
BstBI TT'CG_AA
Cuts at: 6065 6065
Size: 7261
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7261
BstXI CCAn_nnnn'nTGG
Cuts at: 3056 3056
Size: 7261
BstYI r'GATC_y
Cuts at: 1972 2361 4591 4958 5343 5360 6812 6898 6910
Size: 389 2230 367 385 17 1452 86 12
Cuts at: 6910 7256 1972
Size: 346 1977
Fragments arranged by size:
2230 1977 1452 389 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2460 2817 2460
Size: 357 6904
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2048 2217 2490 2979 3255
Size: 552 939 27 17 169 273 489 276
Cuts at: 3255 3972 4365 513
Size: 717 393 3409
Fragments arranged by size:
3409 939 717 552 489 393 276 273
169 27 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6522
Fragments arranged by size:
6522 683 56
BtsI GCAGTG_nn'
Cuts at: 2867 3007 3267 3543 3561 3835 4777 5571 5591
Size: 140 260 276 18 274 942 794 20
Cuts at: 5591 2867
Size: 4537
Fragments arranged by size:
4537 942 794 276 274 260 140 20
18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7226
DraI TTT'AAA
Cuts at: 4565 4565
Size: 7261
DraIII CAC_nnn'GTG
Cuts at: 4043 4043
Size: 7261
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3066 6268 809
Size: 507 1750 3202 1802
Fragments arranged by size:
3202 1802 1750 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2028 2636
Size: 54 1425 50 275 18 177 21 608
Cuts at: 2636 3039 3740 3756 4439 4708 5597 7226 8
Size: 403 701 16 683 269 889 1629 43
Fragments arranged by size:
1629 1425 889 701 683 608 403 275
269 177 54 50 43 21 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 4708 1537
Size: 275 2896 4090
Fragments arranged by size:
4090 2896 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2414 2672 2777 2999 3279 3299 5191
Size: 923 138 258 105 222 280 20 1892
Cuts at: 5191 1353
Size: 3423
Fragments arranged by size:
3423 1892 923 280 258 222 138 105
20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 2639 4376 5848 6222 6368
Size: 1064 138 765 285 1737 1472 374 146
Cuts at: 6368 387
Size: 1280
Fragments arranged by size:
1737 1472 1280 1064 765 374 285 146
138
EcoICRI GAG'CTC
Cuts at: 4174 4174
Size: 7261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2404 2519 2656 2956 2980 3091 3162
Size: 393 772 115 137 300 24 111 71
Cuts at: 3162 3245 3647 3732 5324 5921 6708 6976 1239
Size: 83 402 85 1592 597 787 268 1524
Fragments arranged by size:
1592 1524 787 772 597 402 393 300
268 137 115 111 85 83 71 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3333 3333
Size: 7261
EcoO109I rG'GnC_Cy
Cuts at: 2044 2095 2222 2223 2388 2456 2698 3006 3669
Size: 51 127 1 165 68 242 308 663
Cuts at: 3669 3777 3850 3869 2044
Size: 108 73 19 5436
Fragments arranged by size:
5436 663 308 242 165 127 108 73
68 51 19 1
EcoRI G'AATT_C
Cuts at: 3692 4570 4948 3692
Size: 878 378 6005
Fragments arranged by size:
6005 878 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4006 4006
Size: 7261
FspI TGC'GCA
Cuts at: 2472 5767 2472
Size: 3295 3966
FspAI rTGC'GCAy
Cuts at: 2472 2472
Size: 7261
HaeII r_GCGC'y
Cuts at: 1678 3114 3821 3991 6408 1678
Size: 1436 707 170 2417 2531
Fragments arranged by size:
2531 2417 1436 707 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2072 2092 2104 2346
Size: 32 135 32 488 12 20 12 242
Cuts at: 2346 2378 3842 3874 5902 5934 5976 6008 1373
Size: 32 1464 32 2028 32 42 32 2626
Fragments arranged by size:
2626 2028 1464 488 242 135 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 4605 4851 669
Size: 988 281 1048 1619 246 3079
Fragments arranged by size:
3079 1619 1048 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7261
HpaI GTT'AAC
Cuts at: 4851 4851
Size: 7261
KpnI G_GTAC'C
Cuts at: 4226 4586 4226
Size: 360 6901
MfeI C'AATT_G
Cuts at: 2740 4860 2740
Size: 2120 5141
MluI A'CGCG_T
Cuts at: 7137 7137
Size: 7261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 2778 6375 6559 1305
Size: 948 525 3597 184 2007
Fragments arranged by size:
3597 2007 948 525 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5439
Fragments arranged by size:
5439 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 4106 5219 5578 5737 518
Size: 648 1362 1578 1113 359 159 2042
Fragments arranged by size:
2042 1578 1362 1113 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2050 2151 2809 3087 3349 3595 3825
Size: 427 17 101 658 278 262 246 230
Cuts at: 3825 3974 4094 4153 4207 4468 4677 5354 6502
Size: 149 120 59 54 261 209 677 1148
Cuts at: 6502 6747 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 658 427 278 262 261
246 245 230 209 149 120 101 59
54 17
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 7261
NcoI C'CATG_G
Cuts at: 513 1065 2004 3255 513
Size: 552 939 1251 4519
Fragments arranged by size:
4519 1251 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7261
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 7261
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7261
NotI GC'GGCC_GC
Cuts at: 4708 4708
Size: 7261
NspI r_CATG'y
Cuts at: 1132 1229 4264 4619 6164 1132
Size: 97 3035 355 1545 2229
Fragments arranged by size:
3035 2229 1545 355 97
PasI CC'CwG_GG
Cuts at: 1348 3010 3665 3872 1348
Size: 1662 655 207 4737
Fragments arranged by size:
4737 1662 655 207
PciI A'CATG_T
Cuts at: 4260 6160 4260
Size: 1900 5361
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3872 1108
Size: 2764 4497
PfoI T'CCnGG_A
Cuts at: 2496 2730 2496
Size: 234 7027
PmeI GTTT'AAAC
Cuts at: 4565 4565
Size: 7261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5330 5362 6843 6875 5330
Size: 32 1481 32 5716
Fragments arranged by size:
5716 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2095 2698 3006 3669 3850 2044
Size: 51 603 308 663 181 5455
Fragments arranged by size:
5455 663 603 308 181 51
PshAI GACnn'nnGTC
Cuts at: 1613 2100 1613
Size: 487 6774
PsiI TTA'TAA
Cuts at: 4831 4831
Size: 7261
PspOMI G'GGCC_C
Cuts at: 2222 3013 2222
Size: 791 6470
PstI C_TGCA'G
Cuts at: 830 2354 2454 2808 2874 3282 3364 4086 4613
Size: 1524 100 354 66 408 82 722 527
Cuts at: 4613 830
Size: 3478
Fragments arranged by size:
3478 1524 722 527 408 354 100 82
66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7261
PvuII CAG'CTG
Cuts at: 2809 3087 3595 4094 4153 4207 4468 2809
Size: 278 508 499 59 54 261 5602
Fragments arranged by size:
5602 508 499 278 261 59 54
SacI G_AGCT'C
Cuts at: 4176 4176
Size: 7261
SacII CC_GC'GG
Cuts at: 2034 2051 3975 2034
Size: 17 1924 5320
Fragments arranged by size:
5320 1924 17
SalI G'TCGA_C
Cuts at: 1936 4603 1936
Size: 2667 4594
SanDI GG'GwC_CC
Cuts at: 2698 3006 3669 2698
Size: 308 663 6290
Fragments arranged by size:
6290 663 308
SapI GCTCTTCn'nnn_
Cuts at: 2414 2672 3279 2414
Size: 258 607 6396
Fragments arranged by size:
6396 607 258
SbfI CC_TGCA'GG
Cuts at: 2454 4613 2454
Size: 2159 5102
ScaI AGT'ACT
Cuts at: 4418 5509 7221 4418
Size: 1091 1712 4458
Fragments arranged by size:
4458 1712 1091
SfcI C'TryA_G
Cuts at: 826 1045 2293 2350 2450 2804 2870 3071 3278
Size: 219 1248 57 100 354 66 201 207
Cuts at: 3278 3360 4082 4112 4609 5744 6425 6616 826
Size: 82 722 30 497 1135 681 191 1471
Fragments arranged by size:
1471 1248 1135 722 681 497 354 219
207 201 191 100 82 66 57 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3764 3764
Size: 7261
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7261
SmaI CCC'GGG
Cuts at: 1872 4500 4588 1872
Size: 2628 88 4545
Fragments arranged by size:
4545 2628 88
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2281 2339 4058 5365 6266 6528
Size: 197 926 338 58 1719 1307 901 262
Cuts at: 6528 820
Size: 1553
Fragments arranged by size:
1719 1553 1307 926 901 338 262 197
58
SnaBI TAC'GTA
Cuts at: 493 5052 493
Size: 4559 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7261
SphI G_CATG'C
Cuts at: 1132 4619 1132
Size: 3487 3774
SspI AAT'ATT
Cuts at: 5 52 5185 5
Size: 47 5133 2081
Fragments arranged by size:
5133 2081 47
StuI AGG'CCT
Cuts at: 1517 2337 3222 1517
Size: 820 885 5556
Fragments arranged by size:
5556 885 820
StyI C'CwwG_G
Cuts at: 513 1065 2004 3099 3210 3255 3432 3503 4546
Size: 552 939 1095 111 45 177 71 1043
Cuts at: 4546 4700 513
Size: 154 3074
Fragments arranged by size:
3074 1095 1043 939 552 177 154 111
71 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3280 3388 3959 4300 4508 5288 5305
Size: 1255 1700 108 571 341 208 780 17
Cuts at: 5305 5458 5643 6088 325
Size: 153 185 445 1498
Fragments arranged by size:
1700 1498 1255 780 571 445 341 208
185 153 108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4098 4416 5507
Size: 274 80 33 51 1443 2121 318 1091
Cuts at: 5507 7219 96
Size: 1712 138
Fragments arranged by size:
2121 1712 1443 1091 318 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2618 2639 2650 2671 3231 3263 3310 3342 3372
Size: 21 11 21 560 32 47 32 30
Cuts at: 3372 3404 3535 3567 3694 3726 7221 7253 2618
Size: 32 131 32 127 32 3495 32 2626
Fragments arranged by size:
3495 2626 560 131 127 47 32 32
32 32 32 32 30 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 2785 4274 1316
Size: 1469 1489 4303
Fragments arranged by size:
4303 1489 1469
XbaI T'CTAG_A
Cuts at: 4597 4597
Size: 7261
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7261
XmaI C'CCGG_G
Cuts at: 1870 4498 4586 1870
Size: 2628 88 4545
Fragments arranged by size:
4545 2628 88
XmnI GAAnn'nnTTC
Cuts at: 2327 5390 2327
Size: 3063 4198
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5069 276
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BsmBI BstZ17I EcoRV
FseI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SexAI SfoI SrfI SwaI XcmI