Restriction map for
pFN21AB0428
(Circular) (Six-base) MAPSORT of: pFN21AB0428.seq Check: 7371 from: 1 to: 7261
pFN21AB0428 7261 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 7261
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5071 278
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4604 1937
Size: 2667 4594
Acc65I G'GTAC_C
Cuts at: 4582 4582
Size: 7261
AclI AA'CG_TT
Cuts at: 1563 5388 5761 1563
Size: 3825 373 3063
Fragments arranged by size:
3825 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3102 3585 4048 4468 5324 6708 6976 1632
Size: 1470 483 463 420 856 1384 268 1917
Fragments arranged by size:
1917 1470 1384 856 483 463 420 268
AflII C'TTAA_G
Cuts at: 820 1017 3907 820
Size: 197 2890 4174
Fragments arranged by size:
4174 2890 197
AflIII A'CryG_T
Cuts at: 1203 6160 7137 1203
Size: 4957 977 1327
Fragments arranged by size:
4957 1327 977
AgeI A'CCGG_T
Cuts at: 6092 6092
Size: 7261
AhdI GACnn_n'nnGTC
Cuts at: 5990 5990
Size: 7261
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7261
AlwNI CAG_nnn'CTG
Cuts at: 2203 2333 2817 3341 3653 5004 6576 2203
Size: 130 484 524 312 1351 1572 2888
Fragments arranged by size:
2888 1572 1351 524 484 312 130
ApaLI G'TGCA_C
Cuts at: 2741 4074 4338 4422 5318 6474 2741
Size: 1333 264 84 896 1156 3528
Fragments arranged by size:
3528 1333 1156 896 264 84
ApoI r'AATT_y
Cuts at: 1493 3792 4162 4570 4796 4948 7057 1493
Size: 2299 370 408 226 152 2109 1697
Fragments arranged by size:
2299 2109 1697 408 370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5815 160
Size: 872 4783 1606
Fragments arranged by size:
4783 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
AvaI C'yCGr_G
Cuts at: 1870 1943 4586 1870
Size: 73 2643 4545
Fragments arranged by size:
4545 2643 73
BamHI G'GATC_C
Cuts at: 2583 4591 4958 2583
Size: 2008 367 4886
Fragments arranged by size:
4886 2008 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3442 4582 6037 618
Size: 325 217 627 1655 1140 1455 1842
Fragments arranged by size:
1842 1655 1455 1140 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2603 4244 4580 1391
Size: 1212 1641 336 4072
Fragments arranged by size:
4072 1641 1212 336
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2314 3853 928
Size: 971 415 1539 4336
Fragments arranged by size:
4336 1539 971 415
BbvCI CC'TCA_GC
Cuts at: 3582 3582
Size: 7261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5452 5486 1903
Size: 34 3515 34 3678
Fragments arranged by size:
3678 3515 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3625 4024 4344 5153 6363 3625
Size: 399 320 809 1210 4523
Fragments arranged by size:
4523 1210 809 399 320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7261
BfrBI ATG'CAT
Cuts at: 4006 4006
Size: 7261
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3063 5872
Size: 107 122 71 631 605 337 1054 2809
Cuts at: 5872 136
Size: 1525
Fragments arranged by size:
2809 1525 1054 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2315 2590 3779 7256 2315
Size: 275 1189 3477 2320
Fragments arranged by size:
3477 2320 1189 275
BlpI GC'TnA_GC
Cuts at: 2853 4678 2853
Size: 1825 5436
Bme1580I G_kGCm'C
Cuts at: 1790 2745 4078 4342 4426 5322 6478 1790
Size: 955 1333 264 84 896 1156 2573
Fragments arranged by size:
2573 1333 1156 955 896 264 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7261
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 3015 3244
Size: 416 53 83 399 24 1338 260 229
Cuts at: 3244 3277 4992 5950 442
Size: 33 1715 958 1753
Fragments arranged by size:
1753 1715 1338 958 416 399 260 229
83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2542 2731 2903 3654 4128 5921 1239
Size: 1303 189 172 751 474 1793 2579
Fragments arranged by size:
2579 1793 1303 751 474 189 172
Bpu10I CC'TnA_GC
Cuts at: 2144 2861 3003 3138 3582 2144
Size: 717 142 135 444 5823
Fragments arranged by size:
5823 717 444 142 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4228 5386 6251 6549 4228
Size: 1158 865 298 4940
Fragments arranged by size:
4940 1158 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2560 2753 2921 2954 3242 3272
Size: 617 543 518 193 168 33 288 30
Cuts at: 3272 3443 3925 3943 4360 5924 882
Size: 171 482 18 417 1564 2219
Fragments arranged by size:
2219 1564 617 543 518 482 417 288
193 171 168 33 30 18
BsaAI yAC'GTr
Cuts at: 493 1204 4421 5052 493
Size: 711 3217 631 2702
Fragments arranged by size:
3217 2702 711 631
BsaBI GATnn'nnATC
Cuts at: 7255 7255
Size: 7261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5068 5450 6085 275
Size: 53 83 186 936 3535 382 635 1451
Fragments arranged by size:
3535 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2546 2880 3300 4092 5694 6092 6366 6513 6924
Size: 334 420 792 1602 398 274 147 411
Cuts at: 6924 2546
Size: 2883
Fragments arranged by size:
2883 1602 792 420 411 398 334 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3652 3682 3876 3906 1178
Size: 30 165 30 2249 30 194 30 4533
Fragments arranged by size:
4533 2249 194 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 2762 3128 1186
Size: 1093 483 366 5319
Fragments arranged by size:
5319 1093 483 366
BseYI C'CCAG_C
Cuts at: 2093 2424 2596 2691 6464 6960 2093
Size: 331 172 95 3773 496 2394
Fragments arranged by size:
3773 2394 496 331 172 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3183 1163
Size: 573 1447 5241
Fragments arranged by size:
5241 1447 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4711 5472 6500 1540
Size: 275 186 2710 761 1028 2301
Fragments arranged by size:
2710 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2745 3004 3670 4078 4244 4342 4426 5322 5407
Size: 259 666 408 166 98 84 896 85
Cuts at: 5407 6478 2745
Size: 1071 3528
Fragments arranged by size:
3528 1071 896 666 408 259 166 98
85 84
BsmI GAATG_Cn'
Cuts at: 4772 4865 4772
Size: 93 7168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2603 2745 3004 3670 4078 4244 4342
Size: 399 813 142 259 666 408 166 98
Cuts at: 4342 4426 4580 5322 5407 6478 1391
Size: 84 154 742 85 1071 2174
Fragments arranged by size:
2174 1071 813 742 666 408 399 259
166 154 142 98 85 84
BspEI T'CCGG_A
Cuts at: 2880 3300 6924 2880
Size: 420 3624 3217
Fragments arranged by size:
3624 3217 420
BspHI T'CATG_A
Cuts at: 4465 5150 6880 4465
Size: 685 1730 4846
Fragments arranged by size:
4846 1730 685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 2972 4616 844
Size: 1882 246 1644 3489
Fragments arranged by size:
3489 1882 1644 246
BsrBI CCG'CTC
Cuts at: 1394 1823 2526 2857 2946 5148 1394
Size: 429 703 331 89 2202 3507
Fragments arranged by size:
3507 2202 703 429 331 89
BsrDI GCAATG_nn'
Cuts at: 65 1248 3009 3829 5756 5930 65
Size: 1183 1761 820 1927 174 1396
Fragments arranged by size:
1927 1761 1396 1183 820 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5905 6092 1700
Size: 110 4095 187 2869
Fragments arranged by size:
4095 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3229 96
Size: 3133 4128
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7261
BssSI C'ACGA_G
Cuts at: 2279 5321 6333 2279
Size: 3042 1012 3207
Fragments arranged by size:
3207 3042 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2350 794
Size: 1556 5705
BstBI TT'CG_AA
Cuts at: 6065 6065
Size: 7261
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7261
BstXI CCAn_nnnn'nTGG
Cuts at: 2840 3329 2840
Size: 489 6772
BstYI r'GATC_y
Cuts at: 1972 2223 2315 2583 2590 3163 3779 4591 4958
Size: 251 92 268 7 573 616 812 367
Cuts at: 4958 5343 5360 6812 6898 6910 7256 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 812 616 573 385 367 346
268 251 92 86 17 12 7
Bsu36I CC'TnA_GG
Cuts at: 3865 3865
Size: 7261
BtgI C'CryG_G
Cuts at: 513 1065 2099 2579 3691 513
Size: 552 1034 480 1112 4083
Fragments arranged by size:
4083 1112 1034 552 480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3084 487
Size: 683 56 1858 4664
Fragments arranged by size:
4664 1858 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 3342 4777 5571 5591 2277
Size: 1065 1435 794 20 3947
Fragments arranged by size:
3947 1435 1065 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7226
DraI TTT'AAA
Cuts at: 3400 4565 3400
Size: 1165 6096
DraIII CAC_nnn'GTG
Cuts at: 4024 4024
Size: 7261
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6268 809
Size: 507 4952 1802
Fragments arranged by size:
4952 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2947 3436
Size: 54 1425 50 275 18 180 937 489
Cuts at: 3436 3517 4708 5597 7226 8
Size: 81 1191 889 1629 43
Fragments arranged by size:
1629 1425 1191 937 889 489 275 180
81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4708 1537
Size: 275 2896 4090
Fragments arranged by size:
4090 2896 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2751 2918 2973 3078 3120 3240 3462 3519
Size: 1398 167 55 105 42 120 222 57
Cuts at: 3519 3621 3896 4293 5191 1353
Size: 102 275 397 898 3423
Fragments arranged by size:
3423 1398 898 397 275 222 167 120
105 102 57 55 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5848 6222 6368 387
Size: 1064 138 4259 374 146 1280
Fragments arranged by size:
4259 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4242 4242
Size: 7261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2542 2731 2903 3102 3585 3654 4048
Size: 393 910 189 172 199 483 69 394
Cuts at: 4048 4128 4468 5324 5921 6708 6976 1239
Size: 80 340 856 597 787 268 1524
Fragments arranged by size:
1524 910 856 787 597 483 394 393
340 268 199 189 172 80 69
EcoO109I rG'GnC_Cy
Cuts at: 3184 3372 3184
Size: 188 7073
EcoRI G'AATT_C
Cuts at: 4570 4948 4570
Size: 378 6883
FspI TGC'GCA
Cuts at: 2159 5767 2159
Size: 3608 3653
FspAI rTGC'GCAy
Cuts at: 2159 2159
Size: 7261
HaeII r_GCGC'y
Cuts at: 1678 6408 1678
Size: 4730 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2307 2339 2575 2607 2986
Size: 32 135 32 735 32 236 32 379
Cuts at: 2986 3018 3176 3208 3824 3856 5902 5934 5976
Size: 32 158 32 616 32 2046 32 42
Cuts at: 5976 6008 1373
Size: 32 2626
Fragments arranged by size:
2626 2046 735 616 379 236 158 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3926 4605 4851 669
Size: 988 281 1988 679 246 3079
Fragments arranged by size:
3079 1988 988 679 281 246
HindIII A'AGCT_T
Cuts at: 748 3979 4060 4312 4535 748
Size: 3231 81 252 223 3474
Fragments arranged by size:
3474 3231 252 223 81
HpaI GTT'AAC
Cuts at: 4851 4851
Size: 7261
KpnI G_GTAC'C
Cuts at: 4586 4586
Size: 7261
MfeI C'AATT_G
Cuts at: 4860 4860
Size: 7261
MluI A'CGCG_T
Cuts at: 7137 7137
Size: 7261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2080 2544 2879 3368 4049 6375 6559 1305
Size: 775 464 335 489 681 2326 184 2007
Fragments arranged by size:
2326 2007 775 681 489 464 335 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 3438 3519 10
Size: 54 1425 343 180 1426 81 3752
Fragments arranged by size:
3752 1426 1425 343 180 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4039 4335 4470 5219 5578 5737 518
Size: 648 2873 296 135 749 359 159 2042
Fragments arranged by size:
2873 2042 749 648 359 296 159 135
MspA1I CmG'CkG
Cuts at: 1606 2101 3197 3325 4677 5354 6502 6747 1606
Size: 495 1096 128 1352 677 1148 245 2120
Fragments arranged by size:
2120 1352 1148 1096 677 495 245 128
NcoI C'CATG_G
Cuts at: 513 1065 2579 513
Size: 552 1514 5195
Fragments arranged by size:
5195 1514 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7261
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7261
NotI GC'GGCC_GC
Cuts at: 4708 4708
Size: 7261
NsiI A_TGCA'T
Cuts at: 4008 4008
Size: 7261
NspI r_CATG'y
Cuts at: 1132 1229 2657 4619 6164 1132
Size: 97 1428 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 2430 3647 1348
Size: 1082 1217 4962
Fragments arranged by size:
4962 1217 1082
PciI A'CATG_T
Cuts at: 6160 6160
Size: 7261
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 2258 4024 1108
Size: 981 169 1766 4345
Fragments arranged by size:
4345 1766 981 169
PfoI T'CCnGG_A
Cuts at: 3894 3894
Size: 7261
PmeI GTTT'AAAC
Cuts at: 4565 4565
Size: 7261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5330 5362 6843 6875 5330
Size: 32 1481 32 5716
Fragments arranged by size:
5716 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3184 3372 3184
Size: 188 7073
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7261
PsiI TTA'TAA
Cuts at: 4122 4831 4122
Size: 709 6552
PstI C_TGCA'G
Cuts at: 830 4613 830
Size: 3783 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7261
PvuII CAG'CTG
Cuts at: 3197 3325 3197
Size: 128 7133
SacI G_AGCT'C
Cuts at: 4244 4244
Size: 7261
SacII CC_GC'GG
Cuts at: 2102 2102
Size: 7261
SalI G'TCGA_C
Cuts at: 1936 4603 1936
Size: 2667 4594
SapI GCTCTTCn'nnn_
Cuts at: 2918 3078 3896 2918
Size: 160 818 6283
Fragments arranged by size:
6283 818 160
SbfI CC_TGCA'GG
Cuts at: 4613 4613
Size: 7261
ScaI AGT'ACT
Cuts at: 5509 7221 5509
Size: 1712 5549
SexAI A'CCwGG_T
Cuts at: 4020 4020
Size: 7261
SfcI C'TryA_G
Cuts at: 826 1045 3835 4609 5744 6425 6616 826
Size: 219 2790 774 1135 681 191 1471
Fragments arranged by size:
2790 1471 1135 774 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7261
SmaI CCC'GGG
Cuts at: 1872 4588 1872
Size: 2716 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4243 5365 6266 6528 820
Size: 197 926 1964 336 1122 901 262 1553
Fragments arranged by size:
1964 1553 1122 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 5052 493
Size: 4559 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7261
SphI G_CATG'C
Cuts at: 1132 2657 4619 1132
Size: 1525 1962 3774
Fragments arranged by size:
3774 1962 1525
SspI AAT'ATT
Cuts at: 5 52 5185 5
Size: 47 5133 2081
Fragments arranged by size:
5133 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7261
StyI C'CwwG_G
Cuts at: 513 1065 2163 2579 4557 4700 513
Size: 552 1098 416 1978 143 3074
Fragments arranged by size:
3074 1978 1098 552 416 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2311 2683 2967 3523 4187 4449 5288
Size: 1255 731 372 284 556 664 262 839
Cuts at: 5288 5305 5458 5643 6088 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 839 731 664 556 445 372
284 262 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3229 3751 5507
Size: 274 80 33 51 1443 1252 522 1756
Cuts at: 5507 7219 96
Size: 1712 138
Fragments arranged by size:
1756 1712 1443 1252 522 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7221 7253 7221
Size: 32 7229
Tth111I GACn'n_nGTC
Cuts at: 1316 4546 1316
Size: 3230 4031
XbaI T'CTAG_A
Cuts at: 3166 4597 3166
Size: 1431 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 3028 3689 2090
Size: 938 661 5662
Fragments arranged by size:
5662 938 661
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7261
XmaI C'CCGG_G
Cuts at: 1870 4586 1870
Size: 2716 4545
XmnI GAAnn'nnTTC
Cuts at: 4061 4229 4536 5390 4061
Size: 168 307 854 5932
Fragments arranged by size:
5932 854 307 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5069 276
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SanDI SfiI SfoI SrfI SwaI