Restriction map for
pFN21AB0426
(Circular) (Six-base) MAPSORT of: pFN21AB0426.seq Check: 2513 from: 1 to: 7240
pFN21AB0426 7240 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2255 2255
Size: 7240
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5050 278
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4583 1937
Size: 2646 4594
Acc65I G'GTAC_C
Cuts at: 3617 4561 3617
Size: 944 6296
AclI AA'CG_TT
Cuts at: 1563 5367 5740 1563
Size: 3804 373 3063
Fragments arranged by size:
3804 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3363 5303 6687 6955 1632
Size: 1731 1940 1384 268 1917
Fragments arranged by size:
1940 1917 1731 1384 268
AfeI AGC'GCT
Cuts at: 2588 2588
Size: 7240
AflII C'TTAA_G
Cuts at: 820 1017 2219 820
Size: 197 1202 5841
Fragments arranged by size:
5841 1202 197
AflIII A'CryG_T
Cuts at: 1203 3411 6139 7116 1203
Size: 2208 2728 977 1327
Fragments arranged by size:
2728 2208 1327 977
AgeI A'CCGG_T
Cuts at: 6071 6071
Size: 7240
AhdI GACnn_n'nnGTC
Cuts at: 5969 5969
Size: 7240
AleI CACnn'nnGTG
Cuts at: 1166 3242 1166
Size: 2076 5164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7240
AlwNI CAG_nnn'CTG
Cuts at: 2212 3031 3193 3607 4000 4983 6555 2212
Size: 819 162 414 393 983 1572 2897
Fragments arranged by size:
2897 1572 983 819 414 393 162
ApaLI G'TGCA_C
Cuts at: 5297 6453 5297
Size: 1156 6084
ApoI r'AATT_y
Cuts at: 1493 4115 4549 4775 4927 7036 1493
Size: 2622 434 226 152 2109 1697
Fragments arranged by size:
2622 2109 1697 434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5794 160
Size: 872 4762 1606
Fragments arranged by size:
4762 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
AvaI C'yCGr_G
Cuts at: 1870 1943 2904 4389 4565 1870
Size: 73 961 1485 176 4545
Fragments arranged by size:
4545 1485 961 176 73
BamHI G'GATC_C
Cuts at: 4570 4937 4570
Size: 367 6873
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2780 2986 3437 3617
Size: 325 217 627 229 764 206 451 180
Cuts at: 3617 3648 4561 6016 618
Size: 31 913 1455 1842
Fragments arranged by size:
1842 1455 913 764 627 451 325 229
217 206 180 31
BanII G_rGCy'C
Cuts at: 1391 2089 2283 2550 2648 3757 3885 4559 1391
Size: 698 194 267 98 1109 128 674 4072
Fragments arranged by size:
4072 1109 698 674 267 194 128 98
BbeI G_GCGC'C
Cuts at: 2020 2020
Size: 7240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2427 2803 928
Size: 971 528 376 5365
Fragments arranged by size:
5365 971 528 376
BbvCI CC'TCA_GC
Cuts at: 3894 3894
Size: 7240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5431 5465 1903
Size: 34 3494 34 3678
Fragments arranged by size:
3678 3494 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5132 6342 5132
Size: 1210 6030
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4491 5851 136
Size: 107 122 71 631 605 2819 1360 1525
Fragments arranged by size:
2819 1525 1360 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2520 4476 7235 2520
Size: 1956 2759 2525
Fragments arranged by size:
2759 2525 1956
BlpI GC'TnA_GC
Cuts at: 4657 4657
Size: 7240
Bme1580I G_kGCm'C
Cuts at: 1790 2783 3442 5301 6457 1790
Size: 993 659 1859 1156 2573
Fragments arranged by size:
2573 1859 1156 993 659
BmgBI CAC'GTC
Cuts at: 1318 2914 1318
Size: 1596 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2200 4971 5929
Size: 416 53 83 399 24 783 2771 958
Cuts at: 5929 442
Size: 1753
Fragments arranged by size:
2771 1753 958 783 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3705 3705
Size: 7240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2948 3008 3855 3888 5900 1239
Size: 1709 60 847 33 2012 2579
Fragments arranged by size:
2579 2012 1709 847 60 33
Bpu10I CC'TnA_GC
Cuts at: 2088 3756 3894 2088
Size: 1668 138 5434
Fragments arranged by size:
5434 1668 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2679 3215 3317 4397 5365 6230 6528 2252
Size: 427 536 102 1080 968 865 298 2964
Fragments arranged by size:
2964 1080 968 865 536 427 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3378 3572 4386 5903 882
Size: 617 1879 194 814 1517 2219
Fragments arranged by size:
2219 1879 1517 814 617 194
BsaAI yAC'GTr
Cuts at: 493 1204 4513 5031 493
Size: 711 3309 518 2702
Fragments arranged by size:
3309 2702 711 518
BsaBI GATnn'nnATC
Cuts at: 3509 7234 3509
Size: 3725 3515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2017 5047 5429 6064
Size: 53 83 186 936 484 3030 382 635
Cuts at: 6064 275
Size: 1451
Fragments arranged by size:
3030 1451 936 635 484 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3526 4185 5673 6071 6345 6492 6903 3526
Size: 659 1488 398 274 147 411 3863
Fragments arranged by size:
3863 1488 659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 2612 2642 3861
Size: 30 165 30 1134 30 45 30 1219
Cuts at: 3861 3891 1178
Size: 30 4527
Fragments arranged by size:
4527 1219 1134 165 45 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2417 2480 2516 2636 3590 3981 1186
Size: 1228 3 63 36 120 954 391 4445
Fragments arranged by size:
4445 1228 954 391 120 63 36 3
BseYI C'CCAG_C
Cuts at: 2060 2641 3778 6443 6939 2060
Size: 581 1137 2665 496 2361
Fragments arranged by size:
2665 2361 1137 581 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 2936 3335 3645 1163
Size: 573 594 606 399 310 4758
Fragments arranged by size:
4758 606 594 573 399 310
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2364 4690 5451 6479 1540
Size: 275 186 363 2326 761 1028 2301
Fragments arranged by size:
2326 2301 1028 761 363 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 3885 5301 5386 6457 2283
Size: 1602 1416 85 1071 3066
Fragments arranged by size:
3066 1602 1416 1071 85
BsmI GAATG_Cn'
Cuts at: 3538 4751 4844 3538
Size: 1213 93 5934
Fragments arranged by size:
5934 1213 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2283 2550 2648 2783 3442 3757
Size: 399 299 194 267 98 135 659 315
Cuts at: 3757 3885 4559 5301 5386 6457 1391
Size: 128 674 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 659 399 315 299
267 194 135 128 98 85
BspEI T'CCGG_A
Cuts at: 3526 6903 3526
Size: 3377 3863
BspHI T'CATG_A
Cuts at: 4164 5129 6859 4164
Size: 965 1730 4545
Fragments arranged by size:
4545 1730 965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2181 2196 2255 2739 2985 3078 3357
Size: 1316 21 15 59 484 246 93 279
Cuts at: 3357 4595 844
Size: 1238 3489
Fragments arranged by size:
3489 1316 1238 484 279 246 93 59
21 15
BsrBI CCG'CTC
Cuts at: 1394 1823 4036 4068 5127 1394
Size: 429 2213 32 1059 3507
Fragments arranged by size:
3507 2213 1059 429 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 5735 5909 65
Size: 1183 4487 174 1396
Fragments arranged by size:
4487 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3207 5884 6071 1700
Size: 110 1397 2677 187 2869
Fragments arranged by size:
2869 2677 1397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7240
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7240
BssSI C'ACGA_G
Cuts at: 5300 6312 5300
Size: 1012 6228
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4118 794
Size: 3324 3916
BstBI TT'CG_AA
Cuts at: 2286 6044 2286
Size: 3758 3482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7240
BstXI CCAn_nnnn'nTGG
Cuts at: 4513 4513
Size: 7240
BstYI r'GATC_y
Cuts at: 1972 2520 2607 4434 4476 4570 4937 5322 5339
Size: 548 87 1827 42 94 367 385 17
Cuts at: 5339 6791 6877 6889 7235 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1827 1452 548 385 367 346 94
87 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 3993 3993
Size: 7240
BtgI C'CryG_G
Cuts at: 513 1065 2004 3982 4029 513
Size: 552 939 1978 47 3724
Fragments arranged by size:
3724 1978 939 552 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3068 3731 487
Size: 683 56 1842 663 3996
Fragments arranged by size:
3996 1842 683 663 56
BtsI GCAGTG_nn'
Cuts at: 3140 4341 4756 5550 5570 3140
Size: 1201 415 794 20 4810
Fragments arranged by size:
4810 1201 794 415 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4445 4480 529
Size: 35 3881 35 3289
Fragments arranged by size:
3881 3289 35 35
DraI TTT'AAA
Cuts at: 4544 4544
Size: 7240
DraIII CAC_nnn'GTG
Cuts at: 2071 2071
Size: 7240
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6247 809
Size: 507 4931 1802
Fragments arranged by size:
4931 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2845 2895 3205
Size: 54 1425 50 275 18 1015 50 310
Cuts at: 3205 3507 3697 4059 4483 4687 5576 7205 8
Size: 302 190 362 424 204 889 1629 43
Fragments arranged by size:
1629 1425 1015 889 424 362 310 302
275 204 190 54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4687 1537
Size: 275 2875 4090
Fragments arranged by size:
4090 2875 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2288 2306 3382 5170 1353
Size: 935 18 1076 1788 3423
Fragments arranged by size:
3423 1788 1076 935 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2522 5827 6201 6347 387
Size: 1064 138 933 3305 374 146 1280
Fragments arranged by size:
3305 1280 1064 933 374 146 138
EcoICRI GAG'CTC
Cuts at: 2281 3883 2281
Size: 1602 5638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2948 3008 3363 3855 3888 5303 5900
Size: 393 1316 60 355 492 33 1415 597
Cuts at: 5900 6687 6955 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1415 1316 787 597 492 393 355
268 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 3023 3425 2177
Size: 846 402 5992
Fragments arranged by size:
5992 846 402
EcoO109I rG'GnC_Cy
Cuts at: 2110 2476 2530 2728 2974 3097 3685 3997 4025
Size: 366 54 198 246 123 588 312 28
Cuts at: 4025 2110
Size: 5325
Fragments arranged by size:
5325 588 366 312 246 198 123 54
28
EcoRI G'AATT_C
Cuts at: 4549 4927 4549
Size: 378 6862
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4279 4279
Size: 7240
FseI GG_CCGG'CC
Cuts at: 3211 3211
Size: 7240
FspI TGC'GCA
Cuts at: 5746 5746
Size: 7240
HaeII r_GCGC'y
Cuts at: 1678 2020 2590 3256 6387 1678
Size: 342 570 666 3131 2531
Fragments arranged by size:
3131 2531 666 570 342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 3569 3601 4461
Size: 32 135 32 965 32 1000 32 860
Cuts at: 4461 4493 5881 5913 5955 5987 1373
Size: 32 1388 32 42 32 2626
Fragments arranged by size:
2626 1388 1000 965 860 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2185 4584 4830 669
Size: 988 281 247 2399 246 3079
Fragments arranged by size:
3079 2399 988 281 247 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7240
HpaI GTT'AAC
Cuts at: 4830 4830
Size: 7240
KasI G'GCGC_C
Cuts at: 2016 2016
Size: 7240
KpnI G_GTAC'C
Cuts at: 3621 4565 3621
Size: 944 6296
MfeI C'AATT_G
Cuts at: 4839 4839
Size: 7240
MluI A'CGCG_T
Cuts at: 7116 7116
Size: 7240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 3540 6354 6538 1305
Size: 1161 1074 2814 184 2007
Fragments arranged by size:
2814 2007 1161 1074 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 4061 10
Size: 54 1425 343 1677 552 3189
Fragments arranged by size:
3189 1677 1425 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3242 5198 5557 5716 518
Size: 648 2076 1956 359 159 2042
Fragments arranged by size:
2076 2042 1956 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2140 3166 3639 4656 5333 6481 6726 1606
Size: 534 1026 473 1017 677 1148 245 2120
Fragments arranged by size:
2120 1148 1026 1017 677 534 473 245
NaeI GCC'GGC
Cuts at: 3209 3209
Size: 7240
NarI GG'CG_CC
Cuts at: 2017 2017
Size: 7240
NcoI C'CATG_G
Cuts at: 513 1065 2004 3982 4029 513
Size: 552 939 1978 47 3724
Fragments arranged by size:
3724 1978 939 552 47
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7240
NgoMIV G'CCGG_C
Cuts at: 3207 3207
Size: 7240
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7240
NotI GC'GGCC_GC
Cuts at: 4687 4687
Size: 7240
NspI r_CATG'y
Cuts at: 1132 1229 3415 4598 6143 1132
Size: 97 2186 1183 1545 2229
Fragments arranged by size:
2229 2186 1545 1183 97
PasI CC'CwG_GG
Cuts at: 1348 2776 3442 1348
Size: 1428 666 5146
Fragments arranged by size:
5146 1428 666
PciI A'CATG_T
Cuts at: 3411 6139 3411
Size: 2728 4512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3031 3193 3833 1108
Size: 1923 162 640 4515
Fragments arranged by size:
4515 1923 640 162
PfoI T'CCnGG_A
Cuts at: 2115 2351 3024 2115
Size: 236 673 6331
Fragments arranged by size:
6331 673 236
PmeI GTTT'AAAC
Cuts at: 4544 4544
Size: 7240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5309 5341 6822 6854 5309
Size: 32 1481 32 5695
Fragments arranged by size:
5695 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 2530 2728 2974 3097 3685 3997 2476
Size: 54 198 246 123 588 312 5719
Fragments arranged by size:
5719 588 312 246 198 123 54
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7240
PsiI TTA'TAA
Cuts at: 4810 4810
Size: 7240
PstI C_TGCA'G
Cuts at: 830 2858 4592 830
Size: 2028 1734 3478
Fragments arranged by size:
3478 2028 1734
PvuI CG_AT'CG
Cuts at: 2001 2364 2001
Size: 363 6877
PvuII CAG'CTG
Cuts at: 2140 3166 2140
Size: 1026 6214
SacI G_AGCT'C
Cuts at: 2283 3885 2283
Size: 1602 5638
SalI G'TCGA_C
Cuts at: 1936 4582 1936
Size: 2646 4594
SanDI GG'GwC_CC
Cuts at: 3685 3685
Size: 7240
SapI GCTCTTCn'nnn_
Cuts at: 2288 2288
Size: 7240
SbfI CC_TGCA'GG
Cuts at: 4592 4592
Size: 7240
ScaI AGT'ACT
Cuts at: 5488 7200 5488
Size: 1712 5528
SexAI A'CCwGG_T
Cuts at: 2805 2805
Size: 7240
SfcI C'TryA_G
Cuts at: 826 1045 2428 2854 4588 5723 6404 6595 826
Size: 219 1383 426 1734 1135 681 191 1471
Fragments arranged by size:
1734 1471 1383 1135 681 426 219 191
SfoI GGC'GCC
Cuts at: 2018 2018
Size: 7240
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7240
SmaI CCC'GGG
Cuts at: 1872 2906 4567 1872
Size: 1034 1661 4545
Fragments arranged by size:
4545 1661 1034
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2219 2267 2694 3230 3332 4412
Size: 197 926 276 48 427 536 102 1080
Cuts at: 4412 5344 6245 6507 820
Size: 932 901 262 1553
Fragments arranged by size:
1553 1080 932 926 901 536 427 276
262 197 102 48
SnaBI TAC'GTA
Cuts at: 493 5031 493
Size: 4538 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7240
SphI G_CATG'C
Cuts at: 1132 4598 1132
Size: 3466 3774
SspI AAT'ATT
Cuts at: 5 52 5164 5
Size: 47 5112 2081
Fragments arranged by size:
5112 2081 47
StuI AGG'CCT
Cuts at: 1517 3596 1517
Size: 2079 5161
StyI C'CwwG_G
Cuts at: 513 1065 2004 3433 3982 4029 4679 513
Size: 552 939 1429 549 47 650 3074
Fragments arranged by size:
3074 1429 939 650 552 549 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 3402 4410 5267 5284 5437 5622
Size: 1255 462 1360 1008 857 17 153 185
Cuts at: 5622 6067 325
Size: 445 1498
Fragments arranged by size:
1498 1360 1255 1008 857 462 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2628 5486 7198
Size: 274 80 33 51 1443 651 2858 1712
Cuts at: 7198 96
Size: 138
Fragments arranged by size:
2858 1712 1443 651 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3861 3893 7200 7232 3861
Size: 32 3307 32 3869
Fragments arranged by size:
3869 3307 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7240
XbaI T'CTAG_A
Cuts at: 4576 4576
Size: 7240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 3097 3178 2881
Size: 216 81 6943
Fragments arranged by size:
6943 216 81
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7240
XmaI C'CCGG_G
Cuts at: 1870 2904 4565 1870
Size: 1034 1661 4545
Fragments arranged by size:
4545 1661 1034
XmnI GAAnn'nnTTC
Cuts at: 5369 5369
Size: 7240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5048 276
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SfiI SrfI SwaI