Restriction map for
pFN21AB0424
(Circular) (Six-base) MAPSORT of: pFN21AB0424.seq Check: 2410 from: 1 to: 7222
pFN21AB0424 7222 bp
With 182 enzymes: SgfI *
March 28, 2012 13:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5032 278
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4565 1937
Size: 2628 4594
Acc65I G'GTAC_C
Cuts at: 4543 4543
Size: 7222
AclI AA'CG_TT
Cuts at: 1563 2138 5349 5722 1563
Size: 575 3211 373 3063
Fragments arranged by size:
3211 3063 575 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2172 2334 2612 3346 3829 4376 5285
Size: 514 26 162 278 734 483 547 909
Cuts at: 5285 6669 6937 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 909 734 547 514 483 278
268 162 26
AfeI AGC'GCT
Cuts at: 2159 2159
Size: 7222
AflII C'TTAA_G
Cuts at: 820 1017 4498 820
Size: 197 3481 3544
Fragments arranged by size:
3544 3481 197
AflIII A'CryG_T
Cuts at: 1203 3769 6121 7098 1203
Size: 2566 2352 977 1327
Fragments arranged by size:
2566 2352 1327 977
AgeI A'CCGG_T
Cuts at: 6053 6053
Size: 7222
AhdI GACnn_n'nnGTC
Cuts at: 5951 5951
Size: 7222
AleI CACnn'nnGTG
Cuts at: 1166 3267 3804 1166
Size: 2101 537 4584
Fragments arranged by size:
4584 2101 537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 2439 1184
Size: 948 307 5967
Fragments arranged by size:
5967 948 307
AlwNI CAG_nnn'CTG
Cuts at: 2272 2299 2608 4965 6537 2272
Size: 27 309 2357 1572 2957
Fragments arranged by size:
2957 2357 1572 309 27
ApaLI G'TGCA_C
Cuts at: 3164 3687 3773 3881 5279 6435 3164
Size: 523 86 108 1398 1156 3951
Fragments arranged by size:
3951 1398 1156 523 108 86
ApoI r'AATT_y
Cuts at: 1493 3062 3429 3549 4429 4531 4757 4909 7018
Size: 1569 367 120 880 102 226 152 2109
Cuts at: 7018 1493
Size: 1697
Fragments arranged by size:
2109 1697 1569 880 367 226 152 120
102
AseI AT'TA_AT
Cuts at: 160 1032 5776 160
Size: 872 4744 1606
Fragments arranged by size:
4744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2196 2246 3972 4547 1870
Size: 73 79 174 50 1726 575 4545
Fragments arranged by size:
4545 1726 575 174 79 73 50
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2912 2945 2912
Size: 33 7189
BamHI G'GATC_C
Cuts at: 2881 4552 4919 2881
Size: 1671 367 5184
Fragments arranged by size:
5184 1671 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2848 4543 5998 618
Size: 325 217 627 1061 1695 1455 1842
Fragments arranged by size:
1842 1695 1455 1061 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2805 4541 1391
Size: 1414 1736 4072
Fragments arranged by size:
4072 1736 1414
BbeI G_GCGC'C
Cuts at: 2852 2852
Size: 7222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 2109 4128 928
Size: 971 180 30 2019 4022
Fragments arranged by size:
4022 2019 971 180 30
BbvCI CC'TCA_GC
Cuts at: 2240 2240
Size: 7222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5413 5447 1903
Size: 34 3476 34 3678
Fragments arranged by size:
3678 3476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2689 5114 6324 2689
Size: 2425 1210 3587
Fragments arranged by size:
3587 2425 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7222
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5833 136
Size: 107 122 71 631 605 4161 1525
Fragments arranged by size:
4161 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7217 7217
Size: 7222
BlpI GC'TnA_GC
Cuts at: 4639 4639
Size: 7222
Bme1580I G_kGCm'C
Cuts at: 1790 3042 3168 3691 3777 3885 5283 6439 1790
Size: 1252 126 523 86 108 1398 1156 2573
Fragments arranged by size:
2573 1398 1252 1156 523 126 108 86
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2640 2851 3729
Size: 416 53 83 399 24 1223 211 878
Cuts at: 3729 4953 5911 442
Size: 1224 958 1753
Fragments arranged by size:
1753 1224 1223 958 878 416 399 211
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2892 2892
Size: 7222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2309 2484 2567 2711 3132 3657 4291
Size: 1055 15 175 83 144 421 525 634
Cuts at: 4291 4459 5882 1239
Size: 168 1423 2579
Fragments arranged by size:
2579 1423 1055 634 525 421 175 168
144 83 15
Bpu10I CC'TnA_GC
Cuts at: 2240 2517 3012 2240
Size: 277 495 6450
Fragments arranged by size:
6450 495 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 3474 5347 6212 6510 2420
Size: 1054 1873 865 298 3132
Fragments arranged by size:
3132 1873 1054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3637 3889 4518 5885 882
Size: 617 2138 252 629 1367 2219
Fragments arranged by size:
2219 2138 1367 629 617 252
BsaAI yAC'GTr
Cuts at: 493 1204 5013 493
Size: 711 3809 2702
Fragments arranged by size:
3809 2702 711
BsaBI GATnn'nnATC
Cuts at: 7216 7216
Size: 7222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2849 5029 5411 6046
Size: 53 83 186 936 1316 2180 382 635
Cuts at: 6046 275
Size: 1451
Fragments arranged by size:
2180 1451 1316 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5655 6053 6327 6474 6885 5655
Size: 398 274 147 411 5992
Fragments arranged by size:
5992 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6997
Fragments arranged by size:
6997 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7222
BseYI C'CCAG_C
Cuts at: 2047 2511 3174 6425 6921 2047
Size: 464 663 3251 496 2348
Fragments arranged by size:
3251 2348 663 496 464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2092 2237 2492 1163
Size: 573 356 145 255 5893
Fragments arranged by size:
5893 573 356 255 145
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4672 5433 6461 1540
Size: 275 186 1324 1347 761 1028 2301
Fragments arranged by size:
2301 1347 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2805 3168 3691 3777 3885 4050 5283 5368
Size: 579 363 523 86 108 165 1233 85
Cuts at: 5368 6439 2226
Size: 1071 3009
Fragments arranged by size:
3009 1233 1071 579 523 363 165 108
86 85
BsmI GAATG_Cn'
Cuts at: 4733 4826 4733
Size: 93 7129
BsmBI CGTCTCn'nnnn_
Cuts at: 2546 2546
Size: 7222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2805 3042 3168 3691 3777 3885
Size: 399 436 579 237 126 523 86 108
Cuts at: 3885 4050 4541 5283 5368 6439 1391
Size: 165 491 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 523 491 436 399
237 165 126 108 86 85
BspEI T'CCGG_A
Cuts at: 6885 6885
Size: 7222
BspHI T'CATG_A
Cuts at: 4330 5111 6841 4330
Size: 781 1730 4711
Fragments arranged by size:
4711 1730 781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2923 4577 844
Size: 2079 1654 3489
Fragments arranged by size:
3489 2079 1654
BsrBI CCG'CTC
Cuts at: 1394 1823 5109 1394
Size: 429 3286 3507
Fragments arranged by size:
3507 3286 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3307 5717 5891 65
Size: 1183 2059 2410 174 1396
Fragments arranged by size:
2410 2059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5866 6053 1700
Size: 110 4056 187 2869
Fragments arranged by size:
4056 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7222
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7222
BssSI C'ACGA_G
Cuts at: 5282 6294 5282
Size: 1012 6210
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7222
BstBI TT'CG_AA
Cuts at: 6026 6026
Size: 7222
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7222
BstXI CCAn_nnnn'nTGG
Cuts at: 3950 3950
Size: 7222
BstYI r'GATC_y
Cuts at: 1972 2268 2881 2983 4483 4552 4919 5304 5321
Size: 296 613 102 1500 69 367 385 17
Cuts at: 5321 6773 6859 6871 7217 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1500 1452 613 385 367 346 296
102 86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 2877 2877
Size: 7222
BtgI C'CryG_G
Cuts at: 513 1065 2502 2772 513
Size: 552 1437 270 4963
Fragments arranged by size:
4963 1437 552 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6483
Fragments arranged by size:
6483 683 56
BtsI GCAGTG_nn'
Cuts at: 2475 3391 4738 5532 5552 2475
Size: 916 1347 794 20 4145
Fragments arranged by size:
4145 1347 916 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3289 3324 529
Size: 35 2725 35 4427
Fragments arranged by size:
4427 2725 35 35
DraI TTT'AAA
Cuts at: 4456 4526 4456
Size: 70 7152
DraIII CAC_nnn'GTG
Cuts at: 2845 2845
Size: 7222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6229 809
Size: 507 4913 1802
Fragments arranged by size:
4913 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 4267 4669
Size: 54 1425 50 275 18 540 1897 402
Cuts at: 4669 5558 7187 8
Size: 889 1629 43
Fragments arranged by size:
1897 1629 1425 889 540 402 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4669 1537
Size: 275 2857 4090
Fragments arranged by size:
4090 2857 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 2355 2652 3140 5152 1353
Size: 957 45 297 488 2012 3423
Fragments arranged by size:
3423 2012 957 488 297 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5809 6183 6329 387
Size: 1064 138 4220 374 146 1280
Fragments arranged by size:
4220 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2803 2803
Size: 7222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2172 2294 2309 2334 2484 2567
Size: 393 514 26 122 15 25 150 83
Cuts at: 2567 2612 2711 3132 3346 3657 3829 4291 4376
Size: 45 99 421 214 311 172 462 85
Cuts at: 4376 4459 5285 5882 6669 6937 1239
Size: 83 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 597 514 462 421 393
311 268 214 172 150 122 99 85
83 83 45 26 25 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2332 2332
Size: 7222
EcoO109I rG'GnC_Cy
Cuts at: 2192 2634 3288 3855 2192
Size: 442 654 567 5559
Fragments arranged by size:
5559 654 567 442
EcoRI G'AATT_C
Cuts at: 3549 4531 4909 3549
Size: 982 378 5862
Fragments arranged by size:
5862 982 378
EcoRV GAT'ATC
Cuts at: 4165 4165
Size: 7222
FspI TGC'GCA
Cuts at: 5728 5728
Size: 7222
HaeII r_GCGC'y
Cuts at: 1678 2161 2528 2852 6369 1678
Size: 483 367 324 3517 2531
Fragments arranged by size:
3517 2531 483 367 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2326 2358 2819 2851 3139
Size: 32 135 32 754 32 461 32 288
Cuts at: 3139 3171 3676 3708 4468 4500 5863 5895 5937
Size: 32 505 32 760 32 1363 32 42
Cuts at: 5937 5969 1373
Size: 32 2626
Fragments arranged by size:
2626 1363 760 754 505 461 288 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2938 4566 4812 669
Size: 988 281 1000 1628 246 3079
Fragments arranged by size:
3079 1628 1000 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3673 748
Size: 2925 4297
HpaI GTT'AAC
Cuts at: 4812 4812
Size: 7222
KasI G'GCGC_C
Cuts at: 2848 2848
Size: 7222
KpnI G_GTAC'C
Cuts at: 4547 4547
Size: 7222
MfeI C'AATT_G
Cuts at: 4821 4821
Size: 7222
MluI A'CGCG_T
Cuts at: 3769 7098 3769
Size: 3329 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4082 6336 6520 1305
Size: 2777 2254 184 2007
Fragments arranged by size:
2777 2254 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4269 10
Size: 54 1425 343 2437 2963
Fragments arranged by size:
2963 2437 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2114 3267 3652 3736 3804 4335
Size: 648 843 105 1153 385 84 68 531
Cuts at: 4335 4392 5180 5539 5698 518
Size: 57 788 359 159 2042
Fragments arranged by size:
2042 1153 843 788 648 531 385 359
159 105 84 68 57
MspA1I CmG'CkG
Cuts at: 1606 2051 3160 3279 4437 4638 5315 6463 6708
Size: 445 1109 119 1158 201 677 1148 245
Cuts at: 6708 1606
Size: 2120
Fragments arranged by size:
2120 1158 1148 1109 677 445 245 201
119
NarI GG'CG_CC
Cuts at: 2849 2849
Size: 7222
NcoI C'CATG_G
Cuts at: 513 1065 2772 513
Size: 552 1707 4963
Fragments arranged by size:
4963 1707 552
NdeI CA'TA_TG
Cuts at: 387 3613 387
Size: 3226 3996
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7222
NotI GC'GGCC_GC
Cuts at: 4669 4669
Size: 7222
NspI r_CATG'y
Cuts at: 1132 1229 4580 6125 1132
Size: 97 3351 1545 2229
Fragments arranged by size:
3351 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2328 1348
Size: 980 6242
PciI A'CATG_T
Cuts at: 6121 6121
Size: 7222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 1108
Size: 1536 5686
PfoI T'CCnGG_A
Cuts at: 2271 2544 2688 2271
Size: 273 144 6805
Fragments arranged by size:
6805 273 144
PmeI GTTT'AAAC
Cuts at: 4526 4526
Size: 7222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5291 5323 6804 6836 5291
Size: 32 1481 32 5677
Fragments arranged by size:
5677 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2192 2634 3855 2192
Size: 442 1221 5559
Fragments arranged by size:
5559 1221 442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7222
PsiI TTA'TAA
Cuts at: 4792 4792
Size: 7222
PstI C_TGCA'G
Cuts at: 830 2486 4574 830
Size: 1656 2088 3478
Fragments arranged by size:
3478 2088 1656
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7222
PvuII CAG'CTG
Cuts at: 2051 4437 2051
Size: 2386 4836
SacI G_AGCT'C
Cuts at: 2805 2805
Size: 7222
SalI G'TCGA_C
Cuts at: 1936 4564 1936
Size: 2628 4594
SanDI GG'GwC_CC
Cuts at: 2634 2634
Size: 7222
SapI GCTCTTCn'nnn_
Cuts at: 3140 3140
Size: 7222
SbfI CC_TGCA'GG
Cuts at: 4574 4574
Size: 7222
ScaI AGT'ACT
Cuts at: 5470 7182 5470
Size: 1712 5510
SexAI A'CCwGG_T
Cuts at: 3252 3252
Size: 7222
SfcI C'TryA_G
Cuts at: 826 1045 2482 3598 4570 5705 6386 6577 826
Size: 219 1437 1116 972 1135 681 191 1471
Fragments arranged by size:
1471 1437 1135 1116 972 681 219 191
SfoI GGC'GCC
Cuts at: 2850 2850
Size: 7222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7222
SmaI CCC'GGG
Cuts at: 1872 2198 4549 1872
Size: 326 2351 4545
Fragments arranged by size:
4545 2351 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 3489 4498 5326 6227 6489
Size: 197 926 456 1090 1009 828 901 262
Cuts at: 6489 820
Size: 1553
Fragments arranged by size:
1553 1090 1009 926 901 828 456 262
197
SnaBI TAC'GTA
Cuts at: 493 5013 493
Size: 4520 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7222
SphI G_CATG'C
Cuts at: 1132 4580 1132
Size: 3448 3774
SspI AAT'ATT
Cuts at: 5 52 3440 5146 5
Size: 47 3388 1706 2081
Fragments arranged by size:
3388 2081 1706 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7222
StyI C'CwwG_G
Cuts at: 513 1065 2772 4661 513
Size: 552 1707 1889 3074
Fragments arranged by size:
3074 1889 1707 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2320 2525 2833 3188 3311 5249
Size: 1255 450 290 205 308 355 123 1938
Cuts at: 5249 5266 5419 5604 6049 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1938 1498 1255 450 445 355 308 290
205 185 153 123 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 5468 7180
Size: 274 80 33 51 1443 147 3344 1712
Cuts at: 7180 96
Size: 138
Fragments arranged by size:
3344 1712 1443 274 147 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3611 3643 7182 7214 3611
Size: 32 3539 32 3619
Fragments arranged by size:
3619 3539 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7222
XbaI T'CTAG_A
Cuts at: 4558 4558
Size: 7222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2794 2794
Size: 7222
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7222
XmaI C'CCGG_G
Cuts at: 1870 2196 4547 1870
Size: 326 2351 4545
Fragments arranged by size:
4545 2351 326
XmnI GAAnn'nnTTC
Cuts at: 3317 3484 3674 3731 3842 5351 3317
Size: 167 190 57 111 1509 5188
Fragments arranged by size:
5188 1509 190 167 111 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5030 276
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BfrBI BsiWI BstZ17I
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SfiI SrfI
SwaI