Restriction map for
pFN21AB0421
(Circular) (Six-base) MAPSORT of: pFN21AB0421.seq Check: 4048 from: 1 to: 5935
pFN21AB0421 5935 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2982 3278 1937
Size: 1045 296 4594
Fragments arranged by size:
4594 1045 296
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2509 3051 3998 5382 5650 1632
Size: 877 542 947 1384 268 1917
Fragments arranged by size:
1917 1384 947 877 542 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 2591 4664 2591
Size: 2073 3862
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2532 3071 3678 5250 2532
Size: 539 607 1572 3217
Fragments arranged by size:
3217 1572 607 539
ApaI G_GGCC'C
Cuts at: 3148 3148
Size: 5935
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2382 3260 1870
Size: 73 195 244 878 4545
Fragments arranged by size:
4545 878 244 195 73
BamHI G'GATC_C
Cuts at: 2142 3265 3632 2142
Size: 1123 367 4445
Fragments arranged by size:
4445 1123 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3256 4711 618
Size: 325 217 627 1469 1455 1842
Fragments arranged by size:
1842 1469 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 2517 3148 3223 3254 1391
Size: 838 288 631 75 31 4072
Fragments arranged by size:
4072 838 631 288 75 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2143 2844 3039 3129 928
Size: 971 244 701 195 90 3734
Fragments arranged by size:
3734 971 701 244 195 90
BbvCI CC'TCA_GC
Cuts at: 3108 3108
Size: 5935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4126 4160 1903
Size: 34 2189 34 3678
Fragments arranged by size:
3678 2189 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 3227 4546
Size: 107 122 71 631 605 363 1192 1319
Cuts at: 4546 136
Size: 1525
Fragments arranged by size:
1525 1319 1192 631 605 363 122 107
71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 3352 3352
Size: 5935
Bme1580I G_kGCm'C
Cuts at: 1790 3148 3996 5152 1790
Size: 1358 848 1156 2573
Fragments arranged by size:
2573 1358 1156 848
BmgBI CAC'GTC
Cuts at: 1318 2149 1318
Size: 831 5104
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2833 2842 3666
Size: 416 53 83 399 24 1416 9 824
Cuts at: 3666 4624 442
Size: 958 1753
Fragments arranged by size:
1753 1416 958 824 416 399 83 53
24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2769 4595 1239
Size: 1530 1826 2579
Fragments arranged by size:
2579 1826 1530
Bpu10I CC'TnA_GC
Cuts at: 3108 3108
Size: 5935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3026 4060 4925 5223 2414
Size: 612 1034 865 298 3126
Fragments arranged by size:
3126 1034 865 612 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2385 2578 2599 2629 2952 4598 882
Size: 617 886 193 21 30 323 1646 2219
Fragments arranged by size:
2219 1646 886 617 323 193 30 21
BsaAI yAC'GTr
Cuts at: 493 1204 3726 493
Size: 711 2522 2702
Fragments arranged by size:
2702 2522 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2249 4368 4766 5040 5187 5598 2249
Size: 2119 398 274 147 411 2586
Fragments arranged by size:
2586 2119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5710
Fragments arranged by size:
5710 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2457 2732 1186
Size: 922 349 275 4389
Fragments arranged by size:
4389 922 349 275
BseYI C'CCAG_C
Cuts at: 2363 3086 5138 5634 2363
Size: 723 2052 496 2664
Fragments arranged by size:
2664 2052 723 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2805 3996 4081 5152 2805
Size: 1191 85 1071 3588
Fragments arranged by size:
3588 1191 1071 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 3208 3208
Size: 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2517 2805 3148 3223 3254 3996
Size: 399 439 288 288 343 75 31 742
Cuts at: 3996 4081 5152 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 343 288 288
85 75 31
BspEI T'CCGG_A
Cuts at: 2249 5598 2249
Size: 3349 2586
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3290 844
Size: 2446 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 3822 1394
Size: 429 212 1787 3507
Fragments arranged by size:
3507 1787 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 4579 4766 1700
Size: 110 242 2527 187 2869
Fragments arranged by size:
2869 2527 242 187 110
BsrGI T'GTAC_A
Cuts at: 96 2734 96
Size: 2638 3297
BssHII G'CGCG_C
Cuts at: 1925 2166 1925
Size: 241 5694
BssSI C'ACGA_G
Cuts at: 2081 2228 2449 3995 5007 2081
Size: 147 221 1546 1012 3009
Fragments arranged by size:
3009 1546 1012 221 147
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5935
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 3100 1184
Size: 1916 4019
BstXI CCAn_nnnn'nTGG
Cuts at: 2758 2758
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 2142 3265 3632 4017 4034 5486 5572 5584
Size: 170 1123 367 385 17 1452 86 12
Cuts at: 5584 5930 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1123 385 367 346 170 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2017 2484 2017
Size: 467 5468
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2678 487
Size: 683 56 1452 3744
Fragments arranged by size:
3744 1452 683 56
BtsI GCAGTG_nn'
Cuts at: 3451 4245 4265 3451
Size: 794 20 5121
Fragments arranged by size:
5121 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2657 2692 529
Size: 35 2093 35 3772
Fragments arranged by size:
3772 2093 35 35
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DraIII CAC_nnn'GTG
Cuts at: 2732 2732
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4942 809
Size: 507 3626 1802
Fragments arranged by size:
3626 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2503 2534 2748
Size: 54 1425 50 275 18 673 31 214
Cuts at: 2748 2964 3382 4271 5900 8
Size: 216 418 889 1629 43
Fragments arranged by size:
1629 1425 889 673 418 275 216 214
54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2484 3865 1353
Size: 1131 1381 3423
Fragments arranged by size:
3423 1381 1131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4522 4896 5042 387
Size: 1064 138 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2509 2769 3051 3998 4595 5382 5650
Size: 393 877 260 282 947 597 787 268
Cuts at: 5650 1239
Size: 1524
Fragments arranged by size:
1524 947 877 787 597 393 282 268
260
EcoO109I rG'GnC_Cy
Cuts at: 2275 2275
Size: 5935
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2577 2609 4576 4608 4650
Size: 32 135 32 1005 32 1967 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 1967 1005 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3279 3525 669
Size: 988 281 1341 246 3079
Fragments arranged by size:
3079 1341 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2310 5049 5233 1305
Size: 1005 2739 184 2007
Fragments arranged by size:
2739 2007 1005 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2536 2750 2966 10
Size: 54 1425 343 704 214 216 2979
Fragments arranged by size:
2979 1425 704 343 216 214 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 3893 4252 4411 518
Size: 648 1230 1497 359 159 2042
Fragments arranged by size:
2042 1497 1230 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2163 2743 2789 3351 4028 5176 5421 1606
Size: 557 580 46 562 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 580 562 557 245 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 3293 4838 1132
Size: 97 2064 1545 2229
Fragments arranged by size:
2229 2064 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2554 1108
Size: 1446 4489
PfoI T'CCnGG_A
Cuts at: 2256 2256
Size: 5935
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2275
Size: 5935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PspOMI G'GGCC_C
Cuts at: 3144 3144
Size: 5935
PstI C_TGCA'G
Cuts at: 830 3165 3287 830
Size: 2335 122 3478
Fragments arranged by size:
3478 2335 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2163 2743 2789 2163
Size: 580 46 5309
Fragments arranged by size:
5309 580 46
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3165 3287 3165
Size: 122 5813
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SfcI C'TryA_G
Cuts at: 826 1045 2335 3161 3283 4418 5099 5290 826
Size: 219 1290 826 122 1135 681 191 1471
Fragments arranged by size:
1471 1290 1135 826 681 219 191 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 3005 4039 4940 5202 820
Size: 197 926 486 576 1034 901 262 1553
Fragments arranged by size:
1553 1034 926 901 576 486 262 197
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 2004 2751 3042 3374 513
Size: 552 939 747 291 332 3074
Fragments arranged by size:
3074 939 747 552 332 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2933 3962 3979 4132 4317 4762 325
Size: 1255 1353 1029 17 153 185 445 1498
Fragments arranged by size:
1498 1353 1255 1029 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2734 4181 5893
Size: 274 80 33 51 1443 757 1447 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
1712 1447 1443 757 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2197 2229 2440 2472 2634 2666 5895 5927 2197
Size: 32 211 32 162 32 3229 32 2205
Fragments arranged by size:
3229 2205 211 162 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2180 2605 1316
Size: 864 425 4646
Fragments arranged by size:
4646 864 425
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 4064 4064
Size: 5935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI