Restriction map for
pFN21AB0419
(Circular) (Six-base) MAPSORT of: pFN21AB0419.seq Check: 1178 from: 1 to: 6121
pFN21AB0419 6121 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 2274 4248 4621 1563
Size: 711 1974 373 3063
Fragments arranged by size:
3063 1974 711 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2592 4184 5568 5836 1632
Size: 960 1592 1384 268 1917
Fragments arranged by size:
1917 1592 1384 960 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 3250 3864 5436 3250
Size: 614 1572 3935
Fragments arranged by size:
3935 1572 614
ApaI G_GGCC'C
Cuts at: 3236 3236
Size: 6121
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2109 3088 3148 3430 3656 3808 5917 1493
Size: 616 979 60 282 226 152 2109 1697
Fragments arranged by size:
2109 1697 979 616 282 226 152 60
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3442 4897 618
Size: 325 217 627 1655 1455 1842
Fragments arranged by size:
1842 1655 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2125 3236 3440 1391
Size: 734 1111 204 4072
Fragments arranged by size:
4072 1111 734 204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4013 5223 3293
Size: 720 1210 4191
Fragments arranged by size:
4191 1210 720
BclI T'GATC_A
Cuts at: 1548 2821 1548
Size: 1273 4848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2538 3236 4182 5338 1790
Size: 228 520 698 946 1156 2573
Fragments arranged by size:
2573 1156 946 698 520 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3852 4810 442
Size: 416 53 83 399 24 2435 958 1753
Fragments arranged by size:
2435 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4781 1239
Size: 3542 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2109 4246 5111 5409 2109
Size: 2137 865 298 2821
Fragments arranged by size:
2821 2137 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2784 4784 882
Size: 617 1285 2000 2219
Fragments arranged by size:
2219 2000 1285 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5896
Fragments arranged by size:
5896 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2467 2691 1186
Size: 1281 224 4616
Fragments arranged by size:
4616 1281 224
BseYI C'CCAG_C
Cuts at: 3235 5324 5820 3235
Size: 2089 496 3536
Fragments arranged by size:
3536 2089 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 2495 1163
Size: 573 369 390 4789
Fragments arranged by size:
4789 573 390 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 3571 4332 5360 1540
Size: 275 186 75 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186 75
BsiHKAI G_wGCw'C
Cuts at: 2861 4182 4267 5338 2861
Size: 1321 85 1071 3644
Fragments arranged by size:
3644 1321 1071 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2125 2538 2861 3236 3440 4182
Size: 399 228 107 413 323 375 204 742
Cuts at: 4182 4267 5338 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 413 399 375 323 228
204 107 85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 3016 4010 5740 3016
Size: 994 1730 3397
Fragments arranged by size:
3397 1730 994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3476 844
Size: 2632 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2938 4008 1394
Size: 429 1115 1070 3507
Fragments arranged by size:
3507 1115 1070 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2429 4616 4790 65
Size: 1183 1181 2187 174 1396
Fragments arranged by size:
2187 1396 1183 1181 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2434 4765 4952 1700
Size: 110 624 2331 187 2869
Fragments arranged by size:
2869 2331 624 187 110
BsrGI T'GTAC_A
Cuts at: 96 2875 96
Size: 2779 3342
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 3305 1184
Size: 2121 4000
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116
Size: 1479 367 385 17 1452 86 12 346
Cuts at: 6116 1972
Size: 1977
Fragments arranged by size:
1977 1479 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5569
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 3324 3637 4431 4451 3324
Size: 313 794 20 4994
Fragments arranged by size:
4994 794 313 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2325 2610
Size: 54 1425 50 275 18 243 252 285
Cuts at: 2610 2621 3280 3568 4457 6086 8
Size: 11 659 288 889 1629 43
Fragments arranged by size:
1629 1425 889 659 288 285 275 252
243 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2073 3568 1537
Size: 275 261 1495 4090
Fragments arranged by size:
4090 1495 275 261
EarI CTCTTCn'nnn_
Cuts at: 1353 2782 3086 3146 4051 1353
Size: 1429 304 60 905 3423
Fragments arranged by size:
3423 1429 905 304 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2592 4184 4781 5568 5836 1239
Size: 393 960 1592 597 787 268 1524
Fragments arranged by size:
1592 1524 960 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2627 3232 2627
Size: 605 5516
EcoRI G'AATT_C
Cuts at: 3088 3148 3430 3808 3088
Size: 60 282 378 5401
Fragments arranged by size:
5401 378 282 60
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 4762 4794 4836
Size: 32 135 32 1256 32 1902 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2626 1902 1256 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2603 748
Size: 1855 4266
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 5235 5419 1305
Size: 1080 2850 184 2007
Fragments arranged by size:
2850 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 3282 10
Size: 54 1425 343 780 670 2849
Fragments arranged by size:
2849 1425 780 670 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 3021 3069 3129 4079 4438 4597
Size: 648 1081 774 48 60 950 359 159
Cuts at: 4597 518
Size: 2042
Fragments arranged by size:
2042 1081 950 774 648 359 159 60
48
MspA1I CmG'CkG
Cuts at: 1606 2620 3250 3537 4214 5362 5607 1606
Size: 1014 630 287 677 1148 245 2120
Fragments arranged by size:
2120 1148 1014 677 630 287 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5569
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 2884 3394 3479 5024 1132
Size: 97 1655 510 85 1545 2229
Fragments arranged by size:
2229 1655 1545 510 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3193 1108
Size: 2085 4036
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2627 2627
Size: 6121
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 2922 3691 2922
Size: 769 5352
PspOMI G'GGCC_C
Cuts at: 3232 3232
Size: 6121
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 6089
PstI C_TGCA'G
Cuts at: 830 3473 830
Size: 2643 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 3250 3250
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 3093 3153 3334 3469 4604 5285 5476
Size: 219 2048 60 181 135 1135 681 191
Cuts at: 5476 826
Size: 1471
Fragments arranged by size:
2048 1471 1135 681 219 191 181 135
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2124 4225 5126 5388 820
Size: 197 926 181 2101 901 262 1553
Fragments arranged by size:
2101 1553 926 901 262 197 181
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 2468 1517
Size: 951 5170
StyI C'CwwG_G
Cuts at: 513 1065 3310 3560 513
Size: 552 2245 250 3074
Fragments arranged by size:
3074 2245 552 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 4148 4165 4318 4503 4948 325
Size: 1255 698 1870 17 153 185 445 1498
Fragments arranged by size:
1870 1498 1255 698 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2875 2953 4367
Size: 274 80 33 51 1443 898 78 1414
Cuts at: 4367 6079 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1414 898 274 138 80 78
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2025 2057 2686 2718 6081 6113 2025
Size: 32 629 32 3363 32 2033
Fragments arranged by size:
3363 2033 629 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3251 3251
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 2385 4250 2385
Size: 1865 4256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI