Restriction map for
pFN21AB0415
(Circular) (Six-base) MAPSORT of: pFN21AB0415.seq Check: 3499 from: 1 to: 6109
pFN21AB0415 6109 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3022 3069 3215 4172 5556 5824 1632
Size: 1390 47 146 957 1384 268 1917
Fragments arranged by size:
1917 1390 1384 957 268 146 47
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 5008 5985 1203
Size: 3805 977 1327
Fragments arranged by size:
3805 1327 977
AgeI A'CCGG_T
Cuts at: 3295 4940 3295
Size: 1645 4464
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 2681 3269 1166
Size: 1515 588 4006
Fragments arranged by size:
4006 1515 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2898 1184
Size: 1714 4395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 6077
AlwNI CAG_nnn'CTG
Cuts at: 2678 3852 5424 2678
Size: 1174 1572 3363
Fragments arranged by size:
3363 1572 1174
ApaLI G'TGCA_C
Cuts at: 2321 4166 5322 2321
Size: 1845 1156 3108
Fragments arranged by size:
3108 1845 1156
ApoI r'AATT_y
Cuts at: 1493 3418 3644 3796 5905 1493
Size: 1925 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2146 2345 3331 3434 1870
Size: 73 203 199 986 103 4545
Fragments arranged by size:
4545 986 203 199 103 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3430 4885 618
Size: 325 217 627 1643 1455 1842
Fragments arranged by size:
1842 1643 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2355 2381 2769 2927 3193 3371 3428 1391
Size: 964 26 388 158 266 178 57 4072
Fragments arranged by size:
4072 964 388 266 178 158 57 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2169 2487 3179 928
Size: 971 270 318 692 3858
Fragments arranged by size:
3858 971 692 318 270
BbvCI CC'TCA_GC
Cuts at: 2252 2384 2252
Size: 132 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2086 2120 4300 4334 1903
Size: 34 50 34 65 34 2180 34 3678
Fragments arranged by size:
3678 2180 65 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BfrBI ATG'CAT
Cuts at: 3217 3217
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2042 4720
Size: 107 122 71 631 605 337 33 2678
Cuts at: 4720 136
Size: 1525
Fragments arranged by size:
2678 1525 631 605 337 122 107 71
33
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 2325 2887 2942 4170 5326 1790
Size: 535 562 55 1228 1156 2573
Fragments arranged by size:
2573 1228 1156 562 535 55
BmgBI CAC'GTC
Cuts at: 1318 3177 1318
Size: 1859 4250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 3062 3263
Size: 416 53 83 399 24 774 871 201
Cuts at: 3263 3840 4798 442
Size: 577 958 1753
Fragments arranged by size:
1753 958 871 774 577 416 399 201
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2807 2872 2952 3135 4769 1239
Size: 1568 65 80 183 1634 2579
Fragments arranged by size:
2579 1634 1568 183 80 65
Bpu10I CC'TnA_GC
Cuts at: 2189 2252 2384 2189
Size: 63 132 5914
Fragments arranged by size:
5914 132 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4234 5099 5397 4234
Size: 865 298 4946
Fragments arranged by size:
4946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4772 882
Size: 617 3273 2219
Fragments arranged by size:
3273 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2869 3900 493
Size: 711 1665 1031 2702
Fragments arranged by size:
2702 1665 1031 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2847 3916 4298 4933
Size: 53 83 186 936 1314 1069 382 635
Cuts at: 4933 275
Size: 1451
Fragments arranged by size:
1451 1314 1069 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2370 3295 4542 4940 5214 5361 5772 2370
Size: 925 1247 398 274 147 411 2707
Fragments arranged by size:
2707 1247 925 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2115 2145 2714 2744 1178
Size: 30 165 30 712 30 569 30 4543
Fragments arranged by size:
4543 712 569 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2906 3131 1186
Size: 1177 543 225 4164
Fragments arranged by size:
4164 1177 543 225
BseYI C'CCAG_C
Cuts at: 2216 2308 2312 2355 3305 5312 5808 2216
Size: 92 4 43 950 2007 496 2517
Fragments arranged by size:
2517 2007 950 496 92 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2894 1163
Size: 573 546 612 4378
Fragments arranged by size:
4378 612 573 546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 2478 3559 4320 5348 1540
Size: 275 186 304 173 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 304 275 186 173
BsiHKAI G_wGCw'C
Cuts at: 2190 2325 2420 2448 2769 4170 4255 5326 2190
Size: 135 95 28 321 1401 85 1071 2973
Fragments arranged by size:
2973 1401 1071 321 135 95 85 28
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2325 2355 2381 2420 2448 2769
Size: 399 400 135 30 26 39 28 321
Cuts at: 2769 2887 2927 2942 3193 3371 3428 4170 4255
Size: 118 40 15 251 178 57 742 85
Cuts at: 4255 5326 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 321 251 178
135 118 85 57 40 39 30 28
26 15
BspEI T'CCGG_A
Cuts at: 2370 5772 2370
Size: 3402 2707
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2150 3464 844
Size: 1306 1314 3489
Fragments arranged by size:
3489 1314 1306
BsrBI CCG'CTC
Cuts at: 1394 1823 2057 2856 3996 1394
Size: 429 234 799 1140 3507
Fragments arranged by size:
3507 1140 799 429 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 2395 3147 4604 4778 65
Size: 1183 1147 752 1457 174 1396
Fragments arranged by size:
1457 1396 1183 1147 752 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2197 2300 3295 4753 4940 1700
Size: 110 387 103 995 1458 187 2869
Fragments arranged by size:
2869 1458 995 387 187 110 103
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 2022 1925
Size: 97 6012
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2872 794
Size: 2078 4031
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 2276 3106 3313 2276
Size: 830 207 5072
Fragments arranged by size:
5072 830 207
BstYI r'GATC_y
Cuts at: 1972 3439 3806 4191 4208 5660 5746 5758 6104
Size: 1467 367 385 17 1452 86 12 346
Cuts at: 6104 1972
Size: 1977
Fragments arranged by size:
1977 1467 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2861 2861
Size: 6109
BtgI C'CryG_G
Cuts at: 513 1065 2682 2934 3362 513
Size: 552 1617 252 428 3260
Fragments arranged by size:
3260 1617 552 428 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5370
Fragments arranged by size:
5370 683 56
BtsI GCAGTG_nn'
Cuts at: 2676 3092 3625 4419 4439 2676
Size: 416 533 794 20 4346
Fragments arranged by size:
4346 794 533 416 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2891 2926 529
Size: 35 2327 35 3712
Fragments arranged by size:
3712 2327 35 35
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DraIII CAC_nnn'GTG
Cuts at: 2185 2872 2185
Size: 687 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2043 2302
Size: 54 1425 50 275 18 180 33 259
Cuts at: 2302 2475 2685 3556 4445 6074 8
Size: 173 210 871 889 1629 43
Fragments arranged by size:
1629 1425 889 871 275 259 210 180
173 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2302 2475 3556 1537
Size: 275 490 173 1081 4090
Fragments arranged by size:
4090 1081 490 275 173
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 3041 4039 1353
Size: 1016 672 998 3423
Fragments arranged by size:
3423 1016 998 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2646 3210 4696 5070 5216 387
Size: 1064 138 1057 564 1486 374 146 1280
Fragments arranged by size:
1486 1280 1064 1057 564 374 146 138
EcoICRI GAG'CTC
Cuts at: 2767 2767
Size: 6109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2807 2872 2952 3022 3069 3135 3215
Size: 393 1175 65 80 70 47 66 80
Cuts at: 3215 4172 4769 5556 5824 1239
Size: 957 597 787 268 1524
Fragments arranged by size:
1524 1175 957 787 597 393 268 80
80 70 66 65 47
EcoO109I rG'GnC_Cy
Cuts at: 2408 2818 3358 2408
Size: 410 540 5159
Fragments arranged by size:
5159 540 410
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
EcoRV GAT'ATC
Cuts at: 2774 2774
Size: 6109
FseI GG_CCGG'CC
Cuts at: 2304 2304
Size: 6109
FspI TGC'GCA
Cuts at: 2085 4615 2085
Size: 2530 3579
HaeII r_GCGC'y
Cuts at: 1678 2063 2077 5256 1678
Size: 385 14 3179 2531
Fragments arranged by size:
3179 2531 385 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2714 2746 3314 3346 4750
Size: 32 135 32 1142 32 568 32 1404
Cuts at: 4750 4782 4824 4856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1404 1142 568 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5223 5407 1305
Size: 3918 184 2007
Fragments arranged by size:
3918 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 10
Size: 54 1425 343 855 3432
Fragments arranged by size:
3432 1425 855 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2681 2832 2993 3269 4067 4426 4585
Size: 648 1515 151 161 276 798 359 159
Cuts at: 4585 518
Size: 2042
Fragments arranged by size:
2042 1515 798 648 359 276 161 159
151
MspA1I CmG'CkG
Cuts at: 1606 2312 2700 2972 3241 3525 4202 5350 5595
Size: 706 388 272 269 284 677 1148 245
Cuts at: 5595 1606
Size: 2120
Fragments arranged by size:
2120 1148 706 677 388 284 272 269
245
NaeI GCC'GGC
Cuts at: 2199 2302 2199
Size: 103 6006
NcoI C'CATG_G
Cuts at: 513 1065 2934 513
Size: 552 1869 3688
Fragments arranged by size:
3688 1869 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NgoMIV G'CCGG_C
Cuts at: 2197 2300 2197
Size: 103 6006
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NsiI A_TGCA'T
Cuts at: 3219 3219
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3217 3467 5012 1132
Size: 97 1988 250 1545 2229
Fragments arranged by size:
2229 1988 1545 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2223 1108
Size: 1115 4994
PfoI T'CCnGG_A
Cuts at: 2487 2544 2732 2957 3246 2487
Size: 57 188 225 289 5350
Fragments arranged by size:
5350 289 225 188 57
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PmlI CAC'GTG
Cuts at: 2869 2869
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2408 2818 3358 2408
Size: 410 540 5159
Fragments arranged by size:
5159 540 410
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PspXI vC'TCGA_Gb
Cuts at: 3331 3331
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2206 3461 830
Size: 1376 1255 3478
Fragments arranged by size:
3478 1376 1255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2312 2972 3241 2312
Size: 660 269 5180
Fragments arranged by size:
5180 660 269
SacI G_AGCT'C
Cuts at: 2769 2769
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SanDI GG'GwC_CC
Cuts at: 3358 3358
Size: 6109
SapI GCTCTTCn'nnn_
Cuts at: 2369 2369
Size: 6109
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 2202 2749 3457 4592 5273 5464 826
Size: 219 1157 547 708 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 708 681 547 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 3295 1700
Size: 1595 4514
SmaI CCC'GGG
Cuts at: 1872 2148 3436 1872
Size: 276 1288 4545
Fragments arranged by size:
4545 1288 276
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3331 4213 5114 5376 820
Size: 197 926 1388 882 901 262 1553
Fragments arranged by size:
1553 1388 926 901 882 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3217 3467 1132
Size: 2085 250 3774
Fragments arranged by size:
3774 2085 250
SspI AAT'ATT
Cuts at: 5 52 3031 4033 5
Size: 47 2979 1002 2081
Fragments arranged by size:
2979 2081 1002 47
StuI AGG'CCT
Cuts at: 1517 3256 1517
Size: 1739 4370
StyI C'CwwG_G
Cuts at: 513 1065 2069 2934 2945 3548 513
Size: 552 1004 865 11 603 3074
Fragments arranged by size:
3074 1004 865 603 552 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 4136 4153 4306 4491 4936 325
Size: 1255 593 1963 17 153 185 445 1498
Fragments arranged by size:
1963 1498 1255 593 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4355 6067 96
Size: 274 80 33 51 1443 2378 1712 138
Fragments arranged by size:
2378 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2115 2147 6069 6101 2115
Size: 32 3922 32 2123
Fragments arranged by size:
3922 2123 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 3331 1943
Size: 1388 4721
XmaI C'CCGG_G
Cuts at: 1870 2146 3434 1870
Size: 276 1288 4545
Fragments arranged by size:
4545 1288 276
XmnI GAAnn'nnTTC
Cuts at: 2284 4238 2284
Size: 1954 4155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I EcoNI FalI FspAI KasI NarI
NruI PacI PspOMI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI XcmI