Restriction map for
pFN21AB0413
(Circular) (Six-base) MAPSORT of: pFN21AB0413.seq Check: 6645 from: 1 to: 6106
pFN21AB0413 6106 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2121 2390 2121
Size: 269 5837
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3033 3449 1937
Size: 1096 416 4594
Fragments arranged by size:
4594 1096 416
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4169 5553 5821 1632
Size: 2537 1384 268 1917
Fragments arranged by size:
2537 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 3158 1166
Size: 1992 4114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 2161 2711 2779 3849 5421 2161
Size: 550 68 1070 1572 2846
Fragments arranged by size:
2846 1572 1070 550 68
ApaI G_GGCC'C
Cuts at: 3290 3290
Size: 6106
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2580 3415 3641 3793 5902 1493
Size: 1087 835 226 152 2109 1697
Fragments arranged by size:
2109 1697 1087 835 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2890 3431 1870
Size: 73 947 541 4545
Fragments arranged by size:
4545 947 541 73
BamHI G'GATC_C
Cuts at: 2431 3436 3803 2431
Size: 1005 367 4734
Fragments arranged by size:
4734 1005 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2085 2207 2540 2834
Size: 325 217 627 279 19 122 333 294
Cuts at: 2834 3427 4882 618
Size: 593 1455 1842
Fragments arranged by size:
1842 1455 627 593 333 325 294 279
217 122 19
BanII G_rGCy'C
Cuts at: 1391 3290 3425 1391
Size: 1899 135 4072
Fragments arranged by size:
4072 1899 135
BbeI G_GCGC'C
Cuts at: 2089 2211 2089
Size: 122 5984
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2171 2205 2312 2346 4297 4331 1903
Size: 34 234 34 107 34 1951 34 3678
Fragments arranged by size:
3678 1951 234 107 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 2442 1548
Size: 894 5212
BfrBI ATG'CAT
Cuts at: 2323 2575 2323
Size: 252 5854
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2627 3381 4717
Size: 107 122 71 631 605 955 754 1336
Cuts at: 4717 136
Size: 1525
Fragments arranged by size:
1525 1336 955 754 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2194 2543 3290 4167 5323 1790
Size: 281 123 349 747 877 1156 2573
Fragments arranged by size:
2573 1156 877 747 349 281 123
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2030 2368 3837
Size: 416 53 83 399 24 613 338 1469
Cuts at: 3837 4795 442
Size: 958 1753
Fragments arranged by size:
1753 1469 958 613 416 399 338 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2099 1056
Size: 1043 5063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4766 1239
Size: 3527 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4231 5096 5394 4231
Size: 865 298 4943
Fragments arranged by size:
4943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2251 4769 882
Size: 617 752 2518 2219
Fragments arranged by size:
2518 2219 752 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2086 2208 2756 2824
Size: 53 83 186 936 553 122 548 68
Cuts at: 2824 3330 3366 3913 4295 4930 275
Size: 506 36 547 382 635 1451
Fragments arranged by size:
1451 936 635 553 548 547 506 382
186 122 83 68 53 36
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5881
Fragments arranged by size:
5881 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3245 1186
Size: 2059 4047
BseYI C'CCAG_C
Cuts at: 2070 2480 3289 3361 5309 5805 2070
Size: 410 809 72 1948 496 2371
Fragments arranged by size:
2371 1948 809 496 410 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 2703 1163
Size: 573 357 610 4566
Fragments arranged by size:
4566 610 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2176 2725 3556 4317 5345 1540
Size: 275 186 175 549 831 761 1028 2301
Fragments arranged by size:
2301 1028 831 761 549 275 186 175
BsiHKAI G_wGCw'C
Cuts at: 4167 4252 5323 4167
Size: 85 1071 4950
Fragments arranged by size:
4950 1071 85
BsmI GAATG_Cn'
Cuts at: 2523 3617 3710 2523
Size: 1094 93 4919
Fragments arranged by size:
4919 1094 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2831 2898 3227 2831
Size: 67 329 5710
Fragments arranged by size:
5710 329 67
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2194 2543 3290 3425 4167 4252
Size: 399 281 123 349 747 135 742 85
Cuts at: 4252 5323 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 747 742 399 349 281 135
123 85
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 2565 2656 3063 3995 5725 2565
Size: 91 407 932 1730 2946
Fragments arranged by size:
2946 1730 932 407 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2390 2787 3461 844
Size: 1277 269 397 674 3489
Fragments arranged by size:
3489 1277 674 397 269
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4601 4775 65
Size: 1183 3353 174 1396
Fragments arranged by size:
3353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 3189 4750 4937 1700
Size: 110 329 1050 1561 187 2869
Fragments arranged by size:
2869 1561 1050 329 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2431 3436 3803 4188 4205 5657 5743 5755
Size: 459 1005 367 385 17 1452 86 12
Cuts at: 5755 6101 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1005 459 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2170 2815 2949 513
Size: 552 1105 645 134 3670
Fragments arranged by size:
3670 1105 645 552 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2026 487
Size: 683 56 800 4567
Fragments arranged by size:
4567 800 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 2411 2875 2973 2980 3622 4416 4436 2405
Size: 6 464 98 7 642 794 20 4075
Fragments arranged by size:
4075 794 642 464 98 20 7 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2173 3273
Size: 54 1425 50 275 18 220 123 1100
Cuts at: 3273 3553 4442 6071 8
Size: 280 889 1629 43
Fragments arranged by size:
1629 1425 1100 889 280 275 220 123
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2173 3553 1537
Size: 275 361 1380 4090
Fragments arranged by size:
4090 1380 361 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2234 4036 1353
Size: 881 1802 3423
Fragments arranged by size:
3423 1802 881
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2537 4693 5067 5213 387
Size: 1064 138 948 2156 374 146 1280
Fragments arranged by size:
2156 1280 1064 948 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4169 4766 5553 5821 1239
Size: 393 2537 597 787 268 1524
Fragments arranged by size:
2537 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2516 3104 3131 2516
Size: 588 27 5491
Fragments arranged by size:
5491 588 27
EcoO109I rG'GnC_Cy
Cuts at: 2158 3198 3286 2158
Size: 1040 88 4978
Fragments arranged by size:
4978 1040 88
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2089 2211 2496 2968 5253 1678
Size: 411 122 285 472 2285 2531
Fragments arranged by size:
2531 2285 472 411 285 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2319 2351 2714 2746 4747
Size: 32 135 32 747 32 363 32 2001
Cuts at: 4747 4779 4821 4853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2001 747 363 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KasI G'GCGC_C
Cuts at: 2085 2207 2085
Size: 122 5984
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 3072 5220 5404 1305
Size: 969 798 2148 184 2007
Fragments arranged by size:
2148 2007 969 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2052 10
Size: 54 1425 343 220 4064
Fragments arranged by size:
4064 1425 343 220 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 3107 3158 4064 4423 4582 518
Size: 648 1404 537 51 906 359 159 2042
Fragments arranged by size:
2042 1404 906 648 537 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2044 2172 2711 2817 3522 4199 5347 5592
Size: 438 128 539 106 705 677 1148 245
Cuts at: 5592 1606
Size: 2120
Fragments arranged by size:
2120 1148 705 677 539 438 245 128
106
NaeI GCC'GGC
Cuts at: 3191 3191
Size: 6106
NarI GG'CG_CC
Cuts at: 2086 2208 2086
Size: 122 5984
NcoI C'CATG_G
Cuts at: 513 1065 2949 513
Size: 552 1884 3670
Fragments arranged by size:
3670 1884 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NgoMIV G'CCGG_C
Cuts at: 3189 3189
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 2095 1052
Size: 1043 5063
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NsiI A_TGCA'T
Cuts at: 2325 2577 2325
Size: 252 5854
NspI r_CATG'y
Cuts at: 1132 1229 2575 3464 5009 1132
Size: 97 1346 889 1545 2229
Fragments arranged by size:
2229 1545 1346 889 97
PasI CC'CwG_GG
Cuts at: 1348 2600 1348
Size: 1252 4854
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2743 3282 1108
Size: 1635 539 3932
Fragments arranged by size:
3932 1635 539
PfoI T'CCnGG_A
Cuts at: 2124 2124
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 3198 2158
Size: 1040 5066
PshAI GACnn'nnGTC
Cuts at: 1613 2759 3396 1613
Size: 1146 637 4323
Fragments arranged by size:
4323 1146 637
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 3286 3286
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3458 830
Size: 2628 3478
PvuI CG_AT'CG
Cuts at: 2001 2725 2001
Size: 724 5382
PvuII CAG'CTG
Cuts at: 2711 2711
Size: 6106
SacII CC_GC'GG
Cuts at: 2173 2818 2173
Size: 645 5461
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SanDI GG'GwC_CC
Cuts at: 3198 3198
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SexAI A'CCwGG_T
Cuts at: 3018 3018
Size: 6106
SfcI C'TryA_G
Cuts at: 826 1045 3145 3454 4589 5270 5461 826
Size: 219 2100 309 1135 681 191 1471
Fragments arranged by size:
2100 1471 1135 681 309 219 191
SfoI GGC'GCC
Cuts at: 2087 2209 2087
Size: 122 5984
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4210 5111 5373 820
Size: 197 926 2267 901 262 1553
Fragments arranged by size:
2267 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 2630 1517
Size: 1113 4993
StyI C'CwwG_G
Cuts at: 513 1065 2311 2671 2949 3166 3545 513
Size: 552 1246 360 278 217 379 3074
Fragments arranged by size:
3074 1246 552 379 360 278 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 2551 2810 4133 4150 4303 4488
Size: 1255 474 497 259 1323 17 153 185
Cuts at: 4488 4933 325
Size: 445 1498
Fragments arranged by size:
1498 1323 1255 497 474 445 259 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4352 6064 96
Size: 274 80 33 51 1443 2375 1712 138
Fragments arranged by size:
2375 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6066 6098 6066
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2078 2465 2710 2078
Size: 387 245 5474
Fragments arranged by size:
5474 387 245
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI PacI PmlI PspXI PsrI RsrII
SacI SapI SfiI SrfI SwaI