Restriction map for
pFN21AB0410
(Circular) (Six-base) MAPSORT of: pFN21AB0410.seq Check: 2044 from: 1 to: 7171
pFN21AB0410 7171 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2529 2529
Size: 7171
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4981 278
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4514 1937
Size: 2577 4594
Acc65I G'GTAC_C
Cuts at: 4492 4492
Size: 7171
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3573 3765 5234 6618 6886 1632
Size: 1941 192 1469 1384 268 1917
Fragments arranged by size:
1941 1917 1469 1384 268 192
AfeI AGC'GCT
Cuts at: 2137 2137
Size: 7171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6974
AflIII A'CryG_T
Cuts at: 1203 6070 7047 1203
Size: 4867 977 1327
Fragments arranged by size:
4867 1327 977
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 5900 5900
Size: 7171
AleI CACnn'nnGTG
Cuts at: 1166 2327 1166
Size: 1161 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2282 2345 1184
Size: 1098 63 6010
Fragments arranged by size:
6010 1098 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 2438
Size: 32 7139
AlwNI CAG_nnn'CTG
Cuts at: 2308 3580 4914 6486 2308
Size: 1272 1334 1572 2993
Fragments arranged by size:
2993 1572 1334 1272
ApaI G_GGCC'C
Cuts at: 2037 2133 2037
Size: 96 7075
ApaLI G'TGCA_C
Cuts at: 2288 3116 5228 6384 2288
Size: 828 2112 1156 3075
Fragments arranged by size:
3075 2112 1156 828
ApoI r'AATT_y
Cuts at: 1493 2399 3027 4480 4706 4858 6967 1493
Size: 906 628 1453 226 152 2109 1697
Fragments arranged by size:
2109 1697 1453 906 628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5725 160
Size: 872 4693 1606
Fragments arranged by size:
4693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2125 2296 2702 4496 1870
Size: 73 82 100 171 406 1794 4545
Fragments arranged by size:
4545 1794 406 171 100 82 73
BamHI G'GATC_C
Cuts at: 4501 4868 4501
Size: 367 6804
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 2541 4492 5947 618
Size: 325 217 627 542 212 1951 1455 1842
Fragments arranged by size:
1951 1842 1455 627 542 325 217 212
BanII G_rGCy'C
Cuts at: 1391 2037 2133 2277 3455 3948 4490 1391
Size: 646 96 144 1178 493 542 4072
Fragments arranged by size:
4072 1178 646 542 493 144 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3203 4459 928
Size: 971 1304 1256 3640
Fragments arranged by size:
3640 1304 1256 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2033 2067 2960 2994 5362 5396 1903
Size: 34 96 34 893 34 2368 34 3678
Fragments arranged by size:
3678 2368 893 96 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5063 6273 5063
Size: 1210 5961
BclI T'GATC_A
Cuts at: 1548 3495 1548
Size: 1947 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2021 5782 136
Size: 107 122 71 631 605 349 3761 1525
Fragments arranged by size:
3761 1525 631 605 349 122 107 71
BglII A'GATC_T
Cuts at: 2634 4260 7166 2634
Size: 1626 2906 2639
Fragments arranged by size:
2906 2639 1626
BlpI GC'TnA_GC
Cuts at: 3573 4588 3573
Size: 1015 6156
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2133 2292 2334 3120 5232 6388 1790
Size: 247 96 159 42 786 2112 1156 2573
Fragments arranged by size:
2573 2112 1156 786 247 159 96 42
BmgBI CAC'GTC
Cuts at: 1318 2287 1318
Size: 969 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2741 2937 4902
Size: 416 53 83 399 24 1324 196 1965
Cuts at: 4902 5860 442
Size: 958 1753
Fragments arranged by size:
1965 1753 1324 958 416 399 196 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 3313 3993 4002 4386 5831 1239
Size: 1052 1022 680 9 384 1445 2579
Fragments arranged by size:
2579 1445 1052 1022 680 384 9
Bpu10I CC'TnA_GC
Cuts at: 4236 4236
Size: 7171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5296 6161 6459 5296
Size: 865 298 6008
Fragments arranged by size:
6008 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2517 2651 5834 882
Size: 617 1018 134 3183 2219
Fragments arranged by size:
3183 2219 1018 617 134
BsaAI yAC'GTr
Cuts at: 493 1204 4962 493
Size: 711 3758 2702
Fragments arranged by size:
3758 2702 711
BsaBI GATnn'nnATC
Cuts at: 7165 7165
Size: 7171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4978 5360 5995 275
Size: 53 83 186 936 3445 382 635 1451
Fragments arranged by size:
3445 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4317 5604 6002 6276 6423 6834 4317
Size: 1287 398 274 147 411 4654
Fragments arranged by size:
4654 1287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6946
Fragments arranged by size:
6946 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2084 2345 2740 3934 3956 1186
Size: 895 3 261 395 1194 22 4401
Fragments arranged by size:
4401 1194 895 395 261 22 3
BseYI C'CCAG_C
Cuts at: 2132 2781 6374 6870 2132
Size: 649 3593 496 2433
Fragments arranged by size:
3593 2433 649 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4079 4167 1163
Size: 573 2343 88 4167
Fragments arranged by size:
4167 2343 573 88
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2175 4621 5382 6410 1540
Size: 275 186 15 159 2446 761 1028 2301
Fragments arranged by size:
2446 2301 1028 761 275 186 159 15
BsiHKAI G_wGCw'C
Cuts at: 2277 2292 2829 2973 3120 4242 5232 5317 6388
Size: 15 537 144 147 1122 990 85 1071
Cuts at: 6388 2277
Size: 3060
Fragments arranged by size:
3060 1122 1071 990 537 147 144 85
15
BsmI GAATG_Cn'
Cuts at: 2141 2810 4682 4775 2141
Size: 669 1872 93 4537
Fragments arranged by size:
4537 1872 669 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2133 2277 2292 2334 2829 2973
Size: 399 247 96 144 15 42 495 144
Cuts at: 2973 3120 3455 3948 4242 4490 5232 5317 6388
Size: 147 335 493 294 248 742 85 1071
Cuts at: 6388 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 495 493 399 335 294
248 247 147 144 144 96 85 42
15
BspEI T'CCGG_A
Cuts at: 6834 6834
Size: 7171
BspHI T'CATG_A
Cuts at: 5060 6790 5060
Size: 1730 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2529 2776 3601 4526 844
Size: 1685 247 825 925 3489
Fragments arranged by size:
3489 1685 925 825 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2199 2341 5058 1394
Size: 429 376 142 2717 3507
Fragments arranged by size:
3507 2717 429 376 142
BsrDI GCAATG_nn'
Cuts at: 65 1248 3856 4383 5666 5840 65
Size: 1183 2608 527 1283 174 1396
Fragments arranged by size:
2608 1396 1283 1183 527 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2170 5815 6002 1700
Size: 110 360 3645 187 2869
Fragments arranged by size:
3645 2869 360 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 5231 6243 5231
Size: 1012 6159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3910 794
Size: 3116 4055
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7171
BstXI CCAn_nnnn'nTGG
Cuts at: 3895 3895
Size: 7171
BstYI r'GATC_y
Cuts at: 1972 2634 4260 4408 4501 4868 5253 5270 6722
Size: 662 1626 148 93 367 385 17 1452
Cuts at: 6722 6808 6820 7166 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1626 1452 662 385 367 346 148
93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2463 3746 2463
Size: 1283 5888
BtgI C'CryG_G
Cuts at: 513 1065 2037 2247 2421 2517 2562 4132 513
Size: 552 972 210 174 96 45 1570 3552
Fragments arranged by size:
3552 1570 972 552 210 174 96 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2245 2541 487
Size: 683 56 1019 296 5117
Fragments arranged by size:
5117 1019 683 296 56
BtsI GCAGTG_nn'
Cuts at: 2612 2827 3272 3453 3712 4687 5481 5501 2612
Size: 215 445 181 259 975 794 20 4282
Fragments arranged by size:
4282 975 794 445 259 215 181 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7136
DraI TTT'AAA
Cuts at: 3648 4475 3648
Size: 827 6344
DraIII CAC_nnn'GTG
Cuts at: 2236 2236
Size: 7171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2423 6178 809
Size: 507 1107 3755 1802
Fragments arranged by size:
3755 1802 1107 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2172 3471
Size: 54 1425 50 275 18 183 159 1299
Cuts at: 3471 4303 4618 5507 7136 8
Size: 832 315 889 1629 43
Fragments arranged by size:
1629 1425 1299 889 832 315 275 183
159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2172 4618 1537
Size: 275 201 159 2446 4090
Fragments arranged by size:
4090 2446 275 201 159
EarI CTCTTCn'nnn_
Cuts at: 1353 2911 3996 4158 5101 1353
Size: 1558 1085 162 943 3423
Fragments arranged by size:
3423 1558 1085 943 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2135 5758 6132 6278 387
Size: 1064 138 546 3623 374 146 1280
Fragments arranged by size:
3623 1280 1064 546 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 7171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 3313 3573 3765 3993 4002 4386
Size: 393 659 1022 260 192 228 9 384
Cuts at: 4386 5234 5831 6618 6886 1239
Size: 848 597 787 268 1524
Fragments arranged by size:
1524 1022 848 787 659 597 393 384
268 260 228 192 9
EcoO109I rG'GnC_Cy
Cuts at: 2033 2129 2305 2416 2686 2033
Size: 96 176 111 270 6518
Fragments arranged by size:
6518 270 176 111 96
EcoRI G'AATT_C
Cuts at: 4480 4858 4480
Size: 378 6793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3210 4093 3210
Size: 883 6288
FspI TGC'GCA
Cuts at: 5677 5677
Size: 7171
HaeII r_GCGC'y
Cuts at: 1678 2139 6318 1678
Size: 461 4179 2531
Fragments arranged by size:
4179 2531 461
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3742 3774 5812 5844 5886
Size: 32 135 32 2170 32 2038 32 42
Cuts at: 5886 5918 1373
Size: 32 2626
Fragments arranged by size:
2626 2170 2038 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2623 3517 4515 4761 669
Size: 988 281 685 894 998 246 3079
Fragments arranged by size:
3079 998 988 894 685 281 246
HindIII A'AGCT_T
Cuts at: 748 3681 3734 748
Size: 2933 53 4185
Fragments arranged by size:
4185 2933 53
HpaI GTT'AAC
Cuts at: 3517 4761 3517
Size: 1244 5927
KpnI G_GTAC'C
Cuts at: 4496 4496
Size: 7171
MfeI C'AATT_G
Cuts at: 2919 3279 4770 2919
Size: 360 1491 5320
Fragments arranged by size:
5320 1491 360
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 6285 6469 1305
Size: 1119 3861 184 2007
Fragments arranged by size:
3861 2007 1119 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3473 4305 10
Size: 54 1425 343 1641 832 2876
Fragments arranged by size:
2876 1641 1425 832 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2327 5129 5488 5647 518
Size: 648 1098 63 2802 359 159 2042
Fragments arranged by size:
2802 2042 1098 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2039 2114 2212 2349 2508 2617 2878 3352
Size: 433 75 98 137 159 109 261 474
Cuts at: 3352 3569 4134 4587 5264 6412 6657 1606
Size: 217 565 453 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 474 453 433 261
245 217 159 137 109 98 75
NaeI GCC'GGC
Cuts at: 2172 2172
Size: 7171
NcoI C'CATG_G
Cuts at: 513 1065 2517 2562 513
Size: 552 1452 45 5122
Fragments arranged by size:
5122 1452 552 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7171
NgoMIV G'CCGG_C
Cuts at: 2170 2170
Size: 7171
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 2740 4529 6074 1132
Size: 97 1511 1789 1545 2229
Fragments arranged by size:
2229 1789 1545 1511 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7171
PciI A'CATG_T
Cuts at: 6070 6070
Size: 7171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2308 1108
Size: 1200 5971
PfoI T'CCnGG_A
Cuts at: 3579 4011 3579
Size: 432 6739
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5240 5272 6753 6785 5240
Size: 32 1481 32 5626
Fragments arranged by size:
5626 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2416 2305
Size: 111 7060
PshAI GACnn'nnGTC
Cuts at: 1613 2747 1613
Size: 1134 6037
PsiI TTA'TAA
Cuts at: 4741 4741
Size: 7171
PspOMI G'GGCC_C
Cuts at: 2033 2129 2033
Size: 96 7075
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3304 3336 3304
Size: 32 7139
PstI C_TGCA'G
Cuts at: 830 2187 2607 3298 3915 4523 830
Size: 1357 420 691 617 608 3478
Fragments arranged by size:
3478 1357 691 617 608 420
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 2349 2508 2617 2878 3352 3569 2349
Size: 159 109 261 474 217 5951
Fragments arranged by size:
5951 474 261 217 159 109
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 7171
SacII CC_GC'GG
Cuts at: 2040 4135 2040
Size: 2095 5076
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SbfI CC_TGCA'GG
Cuts at: 3915 4523 3915
Size: 608 6563
ScaI AGT'ACT
Cuts at: 5419 7131 5419
Size: 1712 5459
SexAI A'CCwGG_T
Cuts at: 2307 3528 2307
Size: 1221 5950
SfcI C'TryA_G
Cuts at: 826 1045 2183 2603 3294 3911 4243 4519 5654
Size: 219 1138 420 691 617 332 276 1135
Cuts at: 5654 6335 6526 826
Size: 681 191 1471
Fragments arranged by size:
1471 1138 1135 691 681 617 420 332
276 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2021 2021
Size: 7171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 2027 2127 2298 4498 1872
Size: 155 100 171 2200 4545
Fragments arranged by size:
4545 2200 171 155 100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5275 6176 6438 820
Size: 197 926 3332 901 262 1553
Fragments arranged by size:
3332 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 3826 152
Size: 3674 3497
SphI G_CATG'C
Cuts at: 1132 2740 4529 1132
Size: 1608 1789 3774
Fragments arranged by size:
3774 1789 1608
SspI AAT'ATT
Cuts at: 5 52 5095 5
Size: 47 5043 2081
Fragments arranged by size:
5043 2081 47
StuI AGG'CCT
Cuts at: 1517 3264 3909 1517
Size: 1747 645 4779
Fragments arranged by size:
4779 1747 645
StyI C'CwwG_G
Cuts at: 513 1065 2412 2517 2562 4021 4610 513
Size: 552 1347 105 45 1459 589 3074
Fragments arranged by size:
3074 1459 1347 589 552 105 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 3818 5198 5215 5368 5553 5998
Size: 1255 1215 1023 1380 17 153 185 445
Cuts at: 5998 325
Size: 1498
Fragments arranged by size:
1498 1380 1255 1215 1023 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2499 5417 7129
Size: 274 80 33 51 1443 522 2918 1712
Cuts at: 7129 96
Size: 138
Fragments arranged by size:
2918 1712 1443 522 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7131 7163 7131
Size: 32 7139
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7171
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 2025 2125 2296 4496 1870
Size: 155 100 171 2200 4545
Fragments arranged by size:
4545 2200 171 155 100
XmnI GAAnn'nnTTC
Cuts at: 5300 5300
Size: 7171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4979 276
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI RsrII SanDI SapI
SfoI SrfI SwaI XcmI