Restriction map for
pFN21AB0406
(Circular) (Six-base) MAPSORT of: pFN21AB0406.seq Check: 9515 from: 1 to: 7132
pFN21AB0406 7132 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2077 2077
Size: 7132
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4942 278
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4475 1937
Size: 2538 4594
Acc65I G'GTAC_C
Cuts at: 4453 4453
Size: 7132
AclI AA'CG_TT
Cuts at: 1563 5259 5632 1563
Size: 3696 373 3063
Fragments arranged by size:
3696 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 2799 2850 2966 5195 6579 6847 1632
Size: 896 271 51 116 2229 1384 268 1917
Fragments arranged by size:
2229 1917 1384 896 271 268 116 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6935
AflIII A'CryG_T
Cuts at: 1203 6031 7008 1203
Size: 4828 977 1327
Fragments arranged by size:
4828 1327 977
AgeI A'CCGG_T
Cuts at: 5963 5963
Size: 7132
AhdI GACnn_n'nnGTC
Cuts at: 5861 5861
Size: 7132
AleI CACnn'nnGTG
Cuts at: 1166 2961 1166
Size: 1795 5337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7132
AlwNI CAG_nnn'CTG
Cuts at: 2027 2281 2488 3466 4344 4875 6447 2027
Size: 254 207 978 878 531 1572 2712
Fragments arranged by size:
2712 1572 978 878 531 254 207
ApaI G_GGCC'C
Cuts at: 2977 2977
Size: 7132
ApaLI G'TGCA_C
Cuts at: 2880 5189 6345 2880
Size: 2309 1156 3667
Fragments arranged by size:
3667 2309 1156
ApoI r'AATT_y
Cuts at: 1493 3598 3711 4441 4667 4819 6928 1493
Size: 2105 113 730 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 730 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 5686 160
Size: 872 4654 1606
Fragments arranged by size:
4654 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
AvaI C'yCGr_G
Cuts at: 1870 1943 3048 4457 1870
Size: 73 1105 1409 4545
Fragments arranged by size:
4545 1409 1105 73
BamHI G'GATC_C
Cuts at: 3793 4462 4829 3793
Size: 669 367 6096
Fragments arranged by size:
6096 669 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4453 5908 618
Size: 325 217 627 2666 1455 1842
Fragments arranged by size:
2666 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2304 2977 4451 1391
Size: 913 673 1474 4072
Fragments arranged by size:
4072 1474 913 673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2324 2663 928
Size: 971 425 339 5397
Fragments arranged by size:
5397 971 425 339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5323 5357 1903
Size: 34 3386 34 3678
Fragments arranged by size:
3678 3386 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3873 5024 6234 3873
Size: 1151 1210 4771
Fragments arranged by size:
4771 1210 1151
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7132
BfrBI ATG'CAT
Cuts at: 4325 4325
Size: 7132
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5743 136
Size: 107 122 71 631 605 4071 1525
Fragments arranged by size:
4071 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7127 7127
Size: 7132
BlpI GC'TnA_GC
Cuts at: 4549 4549
Size: 7132
Bme1580I G_kGCm'C
Cuts at: 1790 2884 2977 5193 6349 1790
Size: 1094 93 2216 1156 2573
Fragments arranged by size:
2573 2216 1156 1094 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2627 3254 4863
Size: 416 53 83 399 24 1210 627 1609
Cuts at: 4863 5821 442
Size: 958 1753
Fragments arranged by size:
1753 1609 1210 958 627 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2733 2128
Size: 605 6527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2291 2351 2381 2465 2570 5792 1239
Size: 1049 3 60 30 84 105 3222 2579
Fragments arranged by size:
3222 2579 1049 105 84 60 30 3
Bpu10I CC'TnA_GC
Cuts at: 2745 2745
Size: 7132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2517 3224 5257 6122 6420 2517
Size: 707 2033 865 298 3229
Fragments arranged by size:
3229 2033 865 707 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2218 3565 5795 882
Size: 617 719 1347 2230 2219
Fragments arranged by size:
2230 2219 1347 719 617
BsaAI yAC'GTr
Cuts at: 493 1204 4923 493
Size: 711 3719 2702
Fragments arranged by size:
3719 2702 711
BsaBI GATnn'nnATC
Cuts at: 3792 3798 7126 3792
Size: 6 3328 3798
Fragments arranged by size:
3798 3328 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4939 5321 5956 275
Size: 53 83 186 936 3406 382 635 1451
Fragments arranged by size:
3406 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2227 5565 5963 6237 6384 6795 2227
Size: 3338 398 274 147 411 2564
Fragments arranged by size:
3338 2564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 1178
Size: 30 165 30 672 30 6205
Fragments arranged by size:
6205 672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 1186
Size: 1171 5961
BseYI C'CCAG_C
Cuts at: 6335 6831 6335
Size: 496 6636
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3503 1163
Size: 573 1767 4792
Fragments arranged by size:
4792 1767 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4582 5343 6371 1540
Size: 275 186 2581 761 1028 2301
Fragments arranged by size:
2581 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2884 4100 5193 5278 6349 2304
Size: 580 1216 1093 85 1071 3087
Fragments arranged by size:
3087 1216 1093 1071 580 85
BsmI GAATG_Cn'
Cuts at: 3662 4211 4643 4736 3662
Size: 549 432 93 6058
Fragments arranged by size:
6058 549 432 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2884 2977 4100 4451 5193 5278
Size: 399 514 580 93 1123 351 742 85
Cuts at: 5278 6349 1391
Size: 1071 2174
Fragments arranged by size:
2174 1123 1071 742 580 514 399 351
93 85
BspEI T'CCGG_A
Cuts at: 6795 6795
Size: 7132
BspHI T'CATG_A
Cuts at: 5021 6751 5021
Size: 1730 5402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2077 3525 4487 844
Size: 1233 1448 962 3489
Fragments arranged by size:
3489 1448 1233 962
BsrBI CCG'CTC
Cuts at: 1394 1823 5019 1394
Size: 429 3196 3507
Fragments arranged by size:
3507 3196 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5627 5801 65
Size: 1183 4379 174 1396
Fragments arranged by size:
4379 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5776 5963 1700
Size: 110 3966 187 2869
Fragments arranged by size:
3966 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7132
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7132
BssSI C'ACGA_G
Cuts at: 5192 6204 5192
Size: 1012 6120
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7132
BstBI TT'CG_AA
Cuts at: 4244 5936 4244
Size: 1692 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7132
BstXI CCAn_nnnn'nTGG
Cuts at: 4108 4108
Size: 7132
BstYI r'GATC_y
Cuts at: 1972 2071 3120 3793 4462 4829 5214 5231 6683
Size: 99 1049 673 669 367 385 17 1452
Cuts at: 6683 6769 6781 7127 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1049 673 669 385 367 346
99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2857 2857
Size: 7132
BtgI C'CryG_G
Cuts at: 513 1065 2569 513
Size: 552 1504 5076
Fragments arranged by size:
5076 1504 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6393
Fragments arranged by size:
6393 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 4648 5442 5462 2355
Size: 2293 794 20 4025
Fragments arranged by size:
4025 2293 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7097
DraI TTT'AAA
Cuts at: 2809 4436 2809
Size: 1627 5505
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6139 809
Size: 507 4823 1802
Fragments arranged by size:
4823 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4579 5468
Size: 54 1425 50 275 18 1705 1044 889
Cuts at: 5468 7097 8
Size: 1629 43
Fragments arranged by size:
1705 1629 1425 1044 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4579 1537
Size: 275 2767 4090
Fragments arranged by size:
4090 2767 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4007 5062 1353
Size: 2654 1055 3423
Fragments arranged by size:
3423 2654 1055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5719 6093 6239 387
Size: 1064 138 4130 374 146 1280
Fragments arranged by size:
4130 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2302 2302
Size: 7132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2291 2351 2381 2465 2528 2570
Size: 393 656 3 60 30 84 63 42
Cuts at: 2570 2799 2850 2966 5195 5792 6579 6847 1239
Size: 229 51 116 2229 597 787 268 1524
Fragments arranged by size:
2229 1524 787 656 597 393 268 229
116 84 63 60 51 42 30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2294
Size: 7132
EcoO109I rG'GnC_Cy
Cuts at: 2024 2640 2024
Size: 616 6516
EcoRI G'AATT_C
Cuts at: 4441 4819 4441
Size: 378 6754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 2066
Size: 7132
FspI TGC'GCA
Cuts at: 5638 5638
Size: 7132
HaeII r_GCGC'y
Cuts at: 1678 6279 1678
Size: 4601 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2311 2343 2684 2716 2752
Size: 32 135 32 739 32 341 32 36
Cuts at: 2752 2784 3465 3497 5773 5805 5847 5879 1373
Size: 32 681 32 2276 32 42 32 2626
Fragments arranged by size:
2626 2276 739 681 341 135 42 36
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4178 4476 4722 669
Size: 988 281 2240 298 246 3079
Fragments arranged by size:
3079 2240 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 2013 748
Size: 1265 5867
HpaI GTT'AAC
Cuts at: 4722 4722
Size: 7132
KpnI G_GTAC'C
Cuts at: 4457 4457
Size: 7132
MfeI C'AATT_G
Cuts at: 4731 4731
Size: 7132
MluI A'CGCG_T
Cuts at: 7008 7008
Size: 7132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3152 6246 6430 1305
Size: 1847 3094 184 2007
Fragments arranged by size:
3094 2007 1847 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3537 10
Size: 54 1425 343 1705 3605
Fragments arranged by size:
3605 1705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2961 5090 5449 5608 518
Size: 648 1795 2129 359 159 2042
Fragments arranged by size:
2129 2042 1795 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4548 5225 6373 6618 1606
Size: 2942 677 1148 245 2120
Fragments arranged by size:
2942 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6580
NdeI CA'TA_TG
Cuts at: 387 3999 387
Size: 3612 3520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7132
NotI GC'GGCC_GC
Cuts at: 4579 4579
Size: 7132
NsiI A_TGCA'T
Cuts at: 4327 4327
Size: 7132
NspI r_CATG'y
Cuts at: 1132 1229 2431 2983 4490 6035 1132
Size: 97 1202 552 1507 1545 2229
Fragments arranged by size:
2229 1545 1507 1202 552 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7132
PciI A'CATG_T
Cuts at: 6031 6031
Size: 7132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2650 1108
Size: 1542 5590
PfoI T'CCnGG_A
Cuts at: 2586 2586
Size: 7132
PmeI GTTT'AAAC
Cuts at: 4436 4436
Size: 7132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5201 5233 6714 6746 5201
Size: 32 1481 32 5587
Fragments arranged by size:
5587 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2640 2640
Size: 7132
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7132
PsiI TTA'TAA
Cuts at: 4702 4702
Size: 7132
PspOMI G'GGCC_C
Cuts at: 2973 2973
Size: 7132
PspXI vC'TCGA_Gb
Cuts at: 3048 3048
Size: 7132
PstI C_TGCA'G
Cuts at: 830 2692 3010 4484 830
Size: 1862 318 1474 3478
Fragments arranged by size:
3478 1862 1474 318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7132
SacI G_AGCT'C
Cuts at: 2304 2304
Size: 7132
SalI G'TCGA_C
Cuts at: 1936 4474 1936
Size: 2538 4594
SbfI CC_TGCA'GG
Cuts at: 4484 4484
Size: 7132
ScaI AGT'ACT
Cuts at: 2902 5380 7092 2902
Size: 2478 1712 2942
Fragments arranged by size:
2942 2478 1712
SexAI A'CCwGG_T
Cuts at: 3465 3465
Size: 7132
SfcI C'TryA_G
Cuts at: 826 1045 2613 2688 3006 4480 5615 6296 6487
Size: 219 1568 75 318 1474 1135 681 191
Cuts at: 6487 826
Size: 1471
Fragments arranged by size:
1568 1474 1471 1135 681 318 219 191
75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7132
SmaI CCC'GGG
Cuts at: 1872 4459 1872
Size: 2587 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2532 3048 3239 5236 6137 6399
Size: 197 926 589 516 191 1997 901 262
Cuts at: 6399 820
Size: 1553
Fragments arranged by size:
1997 1553 926 901 589 516 262 197
191
SnaBI TAC'GTA
Cuts at: 493 4923 493
Size: 4430 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7132
SphI G_CATG'C
Cuts at: 1132 2983 4490 1132
Size: 1851 1507 3774
Fragments arranged by size:
3774 1851 1507
SspI AAT'ATT
Cuts at: 5 52 5056 5
Size: 47 5004 2081
Fragments arranged by size:
5004 2081 47
StuI AGG'CCT
Cuts at: 1517 2402 3072 1517
Size: 885 670 5577
Fragments arranged by size:
5577 885 670
StyI C'CwwG_G
Cuts at: 513 1065 2241 2295 3067 4371 4571 513
Size: 552 1176 54 772 1304 200 3074
Fragments arranged by size:
3074 1304 1176 772 552 200 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2448 2973 3983 5159 5176 5329 5514
Size: 1255 868 525 1010 1176 17 153 185
Cuts at: 5514 5959 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1176 1010 868 525 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2900 5378 7090
Size: 274 80 33 51 1443 923 2478 1712
Cuts at: 7090 96
Size: 138
Fragments arranged by size:
2478 1712 1443 923 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 2716 2748 2975 3007 3362 3394 3807
Size: 32 609 32 227 32 355 32 413
Cuts at: 3807 3839 7092 7124 2075
Size: 32 3253 32 2083
Fragments arranged by size:
3253 2083 609 413 355 227 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7132
XbaI T'CTAG_A
Cuts at: 2368 4363 4468 2368
Size: 1995 105 5032
Fragments arranged by size:
5032 1995 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2489 2552 2967 3168 3680 2489
Size: 63 415 201 512 5941
Fragments arranged by size:
5941 512 415 201 63
XhoI C'TCGA_G
Cuts at: 1943 3048 1943
Size: 1105 6027
XmaI C'CCGG_G
Cuts at: 1870 4457 1870
Size: 2587 4545
XmnI GAAnn'nnTTC
Cuts at: 5261 5261
Size: 7132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4940 276
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PsrI PvuII RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI