Restriction map for
pFN21AB0405
(Circular) (Six-base) MAPSORT of: pFN21AB0405.seq Check: 9577 from: 1 to: 6100
pFN21AB0405 6100 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2811 3443 1937
Size: 874 632 4594
Fragments arranged by size:
4594 874 632
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2564 4163 5547 5815 1632
Size: 617 315 1599 1384 268 1917
Fragments arranged by size:
1917 1599 1384 617 315 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 2476 4829 2476
Size: 2353 3747
AleI CACnn'nnGTG
Cuts at: 1166 3192 1166
Size: 2026 4074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AlwNI CAG_nnn'CTG
Cuts at: 3346 3843 5415 3346
Size: 497 1572 4031
Fragments arranged by size:
4031 1572 497
ApaI G_GGCC'C
Cuts at: 2059 2303 2578 2831 3117 2059
Size: 244 275 253 286 5042
Fragments arranged by size:
5042 286 275 253 244
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 2618 3409 3635 3787 5896 1493
Size: 1125 791 226 152 2109 1697
Fragments arranged by size:
2109 1697 1125 791 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2520 3170 3258 3425 1870
Size: 73 577 650 88 167 4545
Fragments arranged by size:
4545 650 577 167 88 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3421 4876 618
Size: 325 217 627 1634 1455 1842
Fragments arranged by size:
1842 1634 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2059 2303 2450 2578 2831 3117 3152 3419
Size: 668 244 147 128 253 286 35 267
Cuts at: 3419 1391
Size: 4072
Fragments arranged by size:
4072 668 286 267 253 244 147 128
35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2661 928
Size: 971 762 4367
Fragments arranged by size:
4367 971 762
BbvCI CC'TCA_GC
Cuts at: 2315 2315
Size: 6100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4291 4325 1903
Size: 34 2354 34 3678
Fragments arranged by size:
3678 2354 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3213 4711 136
Size: 107 122 71 631 605 1541 1498 1525
Fragments arranged by size:
1541 1525 1498 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2070 2303 2578 2743 2831 3005 3117
Size: 269 11 233 275 165 88 174 112
Cuts at: 3117 4161 5317 1790
Size: 1044 1156 2573
Fragments arranged by size:
2573 1156 1044 275 269 233 174 165
112 88 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3831 4789 442
Size: 416 53 83 399 24 2414 958 1753
Fragments arranged by size:
2414 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2307 2307
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2206 2329 4760 1239
Size: 967 123 2431 2579
Fragments arranged by size:
2579 2431 967 123
Bpu10I CC'TnA_GC
Cuts at: 2315 2315
Size: 6100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4225 5090 5388 4225
Size: 865 298 4937
Fragments arranged by size:
4937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4763 882
Size: 617 3264 2219
Fragments arranged by size:
3264 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 2981 6094 2981
Size: 3113 2987
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3907 4289 4924 275
Size: 53 83 186 936 2374 382 635 1451
Fragments arranged by size:
2374 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2377 2407 2852 2882 1178
Size: 30 165 30 974 30 445 30 4396
Fragments arranged by size:
4396 974 445 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3158 3276 1186
Size: 1972 118 4010
Fragments arranged by size:
4010 1972 118
BseYI C'CCAG_C
Cuts at: 2131 2302 2388 2571 2594 3110 3392 5303 5799
Size: 171 86 183 23 516 282 1911 496
Cuts at: 5799 2131
Size: 2432
Fragments arranged by size:
2432 1911 516 496 282 183 171 86
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3216 3332 1163
Size: 573 1480 116 3931
Fragments arranged by size:
3931 1480 573 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2353 4161 4246 5317 2353
Size: 1808 85 1071 3136
Fragments arranged by size:
3136 1808 1071 85
BsmI GAATG_Cn'
Cuts at: 2130 3611 3704 2130
Size: 1481 93 4526
Fragments arranged by size:
4526 1481 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2070 2303 2353 2450 2578 2743
Size: 399 269 11 233 50 97 128 165
Cuts at: 2743 2831 3005 3117 3152 3419 4161 4246 5317
Size: 88 174 112 35 267 742 85 1071
Cuts at: 5317 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 269 267 233 174
165 128 112 97 88 85 50 35
11
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3455 844
Size: 2611 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3987 1394
Size: 429 2164 3507
Fragments arranged by size:
3507 2164 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2814 4595 4769 65
Size: 1183 1566 1781 174 1396
Fragments arranged by size:
1781 1566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3051 4744 4931 1700
Size: 110 1241 1693 187 2869
Fragments arranged by size:
2869 1693 1241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6100
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 2527 1184
Size: 1343 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 2333 2608 2333
Size: 275 5825
BstYI r'GATC_y
Cuts at: 1972 2628 2976 3430 3797 4182 4199 5651 5737
Size: 656 348 454 367 385 17 1452 86
Cuts at: 5737 5749 6095 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 656 454 385 367 348 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3384 3384
Size: 6100
BtgI C'CryG_G
Cuts at: 513 1065 2004 2332 513
Size: 552 939 328 4281
Fragments arranged by size:
4281 939 552 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3208 487
Size: 683 56 1982 3379
Fragments arranged by size:
3379 1982 683 56
BtsI GCAGTG_nn'
Cuts at: 2547 3187 3199 3616 4410 4430 2547
Size: 640 12 417 794 20 4217
Fragments arranged by size:
4217 794 640 417 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3217 3252 529
Size: 35 2653 35 3377
Fragments arranged by size:
3377 2653 35 35
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 3083 3205
Size: 54 1425 50 275 18 841 412 122
Cuts at: 3205 3547 4436 6065 8
Size: 342 889 1629 43
Fragments arranged by size:
1629 1425 889 841 412 342 275 122
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 3039 4030 1353
Size: 1593 93 991 3423
Fragments arranged by size:
3423 1593 991 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2178 2385 4687 5061 5207 387
Size: 1064 138 589 207 2302 374 146 1280
Fragments arranged by size:
2302 1280 1064 589 374 207 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2249 2329 2564 4163 4760 5547
Size: 393 574 43 80 235 1599 597 787
Cuts at: 5547 5815 1239
Size: 268 1524
Fragments arranged by size:
1599 1524 787 597 574 393 268 235
80 43
EcoO109I rG'GnC_Cy
Cuts at: 2048 2056 2575 2827 2828 3114 2048
Size: 8 519 252 1 286 5034
Fragments arranged by size:
5034 519 286 252 8 1
EcoRI G'AATT_C
Cuts at: 2618 3409 3787 2618
Size: 791 378 4931
Fragments arranged by size:
4931 791 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 6100
FspI TGC'GCA
Cuts at: 2546 4606 2546
Size: 2060 4040
HaeII r_GCGC'y
Cuts at: 1678 2344 2587 3052 5247 1678
Size: 666 243 465 2195 2531
Fragments arranged by size:
2531 2195 666 465 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 2462 2494 2751
Size: 32 135 32 805 32 53 32 257
Cuts at: 2751 2783 2867 2899 3038 3070 4741 4773 4815
Size: 32 84 32 139 32 1671 32 42
Cuts at: 4815 4847 1373
Size: 32 2626
Fragments arranged by size:
2626 1671 805 257 139 135 84 53
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2361 3245 3444 3690 669
Size: 988 281 423 884 199 246 3079
Fragments arranged by size:
3079 988 884 423 281 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2829 5214 5398 1305
Size: 1524 2385 184 2007
Fragments arranged by size:
2385 2007 1524 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2673 3085 10
Size: 54 1425 343 841 412 3025
Fragments arranged by size:
3025 1425 841 412 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2894 3192 4058 4417 4576 518
Size: 648 1728 298 866 359 159 2042
Fragments arranged by size:
2042 1728 866 648 359 298 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2131 2232 2260 2571 3110 3250 3379
Size: 495 30 101 28 311 539 140 129
Cuts at: 3379 3516 4193 5341 5586 1606
Size: 137 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 539 495 311 245 140
137 129 101 30 28
NcoI C'CATG_G
Cuts at: 513 1065 2004 2332 513
Size: 552 939 328 4281
Fragments arranged by size:
4281 939 552 328
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2426 3458 5003 1132
Size: 97 1197 1032 1545 2229
Fragments arranged by size:
2229 1545 1197 1032 97
PasI CC'CwG_GG
Cuts at: 1348 2038 2296 2482 2824 1348
Size: 690 258 186 342 4624
Fragments arranged by size:
4624 690 342 258 186
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2044 3358 1108
Size: 936 1314 3850
Fragments arranged by size:
3850 1314 936
PfoI T'CCnGG_A
Cuts at: 2183 2985 2183
Size: 802 5298
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspOMI G'GGCC_C
Cuts at: 2055 2299 2574 2827 3113 2055
Size: 244 275 253 286 5042
Fragments arranged by size:
5042 286 275 253 244
PstI C_TGCA'G
Cuts at: 830 2436 3194 3452 830
Size: 1606 758 258 3478
Fragments arranged by size:
3478 1606 758 258
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2131 2232 2260 3110 3250 3379 2131
Size: 101 28 850 140 129 4852
Fragments arranged by size:
4852 850 140 129 101 28
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SapI GCTCTTCn'nnn_
Cuts at: 3039 3039
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2432 3190 3448 4583 5264 5455 826
Size: 219 1387 758 258 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 758 681 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 2522 3172 3427 1872
Size: 650 650 255 4545
Fragments arranged by size:
4545 650 650 255
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4204 5105 5367 820
Size: 197 926 2261 901 262 1553
Fragments arranged by size:
2261 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 2018 1517
Size: 501 5599
StyI C'CwwG_G
Cuts at: 513 1065 2004 2051 2332 2682 3539 513
Size: 552 939 47 281 350 857 3074
Fragments arranged by size:
3074 939 857 552 350 281 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2134 2228 2466 2608 2703 2837
Size: 1255 465 89 94 238 142 95 134
Cuts at: 2837 4127 4144 4297 4482 4927 325
Size: 1290 17 153 185 445 1498
Fragments arranged by size:
1498 1290 1255 465 445 238 185 153
142 134 95 94 89 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4346 6058 96
Size: 274 80 33 51 1443 2369 1712 138
Fragments arranged by size:
2369 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2852 2884 6060 6092 2852
Size: 32 3176 32 2860
Fragments arranged by size:
3176 2860 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2365 1316
Size: 1049 5051
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 2520 3170 3425 1870
Size: 650 650 255 4545
Fragments arranged by size:
4545 650 650 255
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI