Restriction map for
pFN21AB0170
(Circular) (Six-base) MAPSORT of: pFN21AB0170.seq Check: 7957 from: 1 to: 7918
pFN21AB0170 7918 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5072 5072
Size: 7918
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2345 5728 278
Size: 53 83 186 936 809 3383 2468
Fragments arranged by size:
3383 2468 936 809 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2193 4164 5261 1937
Size: 256 1971 1097 4594
Fragments arranged by size:
4594 1971 1097 256
Acc65I G'GTAC_C
Cuts at: 5239 5239
Size: 7918
AclI AA'CG_TT
Cuts at: 1563 6045 6418 1563
Size: 4482 373 3063
Fragments arranged by size:
4482 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2819 3099 3710 3863 4544 4910 5981 7365
Size: 1187 280 611 153 681 366 1071 1384
Cuts at: 7365 7633 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1187 1071 681 611 366 280
268 153
AflII C'TTAA_G
Cuts at: 820 1017 2993 4949 820
Size: 197 1976 1956 3789
Fragments arranged by size:
3789 1976 1956 197
AflIII A'CryG_T
Cuts at: 1203 3975 6817 7794 1203
Size: 2772 2842 977 1327
Fragments arranged by size:
2842 2772 1327 977
AgeI A'CCGG_T
Cuts at: 6749 6749
Size: 7918
AhdI GACnn_n'nnGTC
Cuts at: 6647 6647
Size: 7918
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2193 1184
Size: 1009 6909
AlwNI CAG_nnn'CTG
Cuts at: 2416 5661 7233 2416
Size: 3245 1572 3101
Fragments arranged by size:
3245 3101 1572
ApaLI G'TGCA_C
Cuts at: 3930 5975 7131 3930
Size: 2045 1156 4717
Fragments arranged by size:
4717 2045 1156
ApoI r'AATT_y
Cuts at: 1493 2207 2247 2606 2647 3105 3332 3358 3481
Size: 714 40 359 41 458 227 26 123
Cuts at: 3481 3683 3716 3843 4140 4327 5075 5227 5453
Size: 202 33 127 297 187 748 152 226
Cuts at: 5453 5605 7714 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 748 714 458 359 297 227
226 202 187 152 152 127 123 41
40 33 26
AseI AT'TA_AT
Cuts at: 160 1032 2741 3260 6472 160
Size: 872 1709 519 3212 1606
Fragments arranged by size:
3212 1709 1606 872 519
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
AvaI C'yCGr_G
Cuts at: 1870 1943 5243 1870
Size: 73 3300 4545
Fragments arranged by size:
4545 3300 73
BamHI G'GATC_C
Cuts at: 5248 5615 5248
Size: 367 7551
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5239 6694 618
Size: 325 217 627 3452 1455 1842
Fragments arranged by size:
3452 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 3676 5237 1391
Size: 737 1548 1561 4072
Fragments arranged by size:
4072 1561 1548 737
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3698 5094 928
Size: 971 1799 1396 3752
Fragments arranged by size:
3752 1799 1396 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3781 3815 6109 6143 1903
Size: 34 50 34 1760 34 2294 34 3678
Fragments arranged by size:
3678 2294 1760 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3337 5810 7020 3337
Size: 2473 1210 4235
Fragments arranged by size:
4235 2473 1210
BclI T'GATC_A
Cuts at: 1548 3004 3310 1548
Size: 1456 306 6156
Fragments arranged by size:
6156 1456 306
BfrBI ATG'CAT
Cuts at: 3529 3724 4828 4832 3529
Size: 195 1104 4 6615
Fragments arranged by size:
6615 1104 195 4
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6529 136
Size: 107 122 71 631 605 4857 1525
Fragments arranged by size:
4857 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7913 7913
Size: 7918
BlpI GC'TnA_GC
Cuts at: 5335 5335
Size: 7918
Bme1580I G_kGCm'C
Cuts at: 1790 3934 5979 7135 1790
Size: 2144 2045 1156 2573
Fragments arranged by size:
2573 2144 2045 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5649 6607 442
Size: 416 53 83 399 24 4232 958 1753
Fragments arranged by size:
4232 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4757 6578 1239
Size: 3518 1821 2579
Fragments arranged by size:
3518 2579 1821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2127 2545 4965 6043 6908 7206 2127
Size: 418 2420 1078 865 298 2839
Fragments arranged by size:
2839 2420 1078 865 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2726 3722 6581 882
Size: 617 1227 996 2859 2219
Fragments arranged by size:
2859 2219 1227 996 617
BsaAI yAC'GTr
Cuts at: 493 1204 5709 493
Size: 711 4505 2702
Fragments arranged by size:
4505 2702 711
BsaBI GATnn'nnATC
Cuts at: 7912 7912
Size: 7918
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2342 5725 6107 6742
Size: 53 83 186 936 809 3383 382 635
Cuts at: 6742 275
Size: 1451
Fragments arranged by size:
3383 1451 936 809 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6351 6749 7023 7170 7581 6351
Size: 398 274 147 411 6688
Fragments arranged by size:
6688 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3309 3339 1178
Size: 30 165 30 1906 30 5757
Fragments arranged by size:
5757 1906 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7918
BseYI C'CCAG_C
Cuts at: 2127 4381 4585 7121 7617 2127
Size: 2254 204 2536 496 2428
Fragments arranged by size:
2536 2428 2254 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2968 1163
Size: 573 1232 6113
Fragments arranged by size:
6113 1232 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5368 6129 7157 1540
Size: 275 186 3367 761 1028 2301
Fragments arranged by size:
3367 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3676 3934 5979 6064 7135 3676
Size: 258 2045 85 1071 4459
Fragments arranged by size:
4459 2045 1071 258 85
BsmI GAATG_Cn'
Cuts at: 4493 4589 5429 5522 4493
Size: 96 840 93 6889
Fragments arranged by size:
6889 840 96 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 3676 3934 5237 5979 6064 7135
Size: 399 338 1548 258 1303 742 85 1071
Cuts at: 7135 1391
Size: 2174
Fragments arranged by size:
2174 1548 1303 1071 742 399 338 258
85
BspEI T'CCGG_A
Cuts at: 7581 7581
Size: 7918
BspHI T'CATG_A
Cuts at: 3336 5807 7537 3336
Size: 2471 1730 3717
Fragments arranged by size:
3717 2471 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5072 5273 844
Size: 4228 201 3489
Fragments arranged by size:
4228 3489 201
BsrBI CCG'CTC
Cuts at: 1394 1823 5805 1394
Size: 429 3982 3507
Fragments arranged by size:
3982 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 2687 3531 6413 6587 65
Size: 1183 1360 79 844 2882 174 1396
Fragments arranged by size:
2882 1396 1360 1183 844 174 79
BsrFI r'CCGG_y
Cuts at: 1700 1810 6562 6749 1700
Size: 110 4752 187 2869
Fragments arranged by size:
4752 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2668 3639 96
Size: 2572 971 4375
Fragments arranged by size:
4375 2572 971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7918
BssSI C'ACGA_G
Cuts at: 5978 6990 5978
Size: 1012 6906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2036 4595 794
Size: 1242 2559 4117
Fragments arranged by size:
4117 2559 1242
BstBI TT'CG_AA
Cuts at: 2785 3714 6722 2785
Size: 929 3008 3981
Fragments arranged by size:
3981 3008 929
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7918
BstXI CCAn_nnnn'nTGG
Cuts at: 4030 4208 4030
Size: 178 7740
BstYI r'GATC_y
Cuts at: 1972 3571 4045 5248 5615 6000 6017 7469 7555
Size: 1599 474 1203 367 385 17 1452 86
Cuts at: 7555 7567 7913 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1599 1452 1203 474 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 4165 4165
Size: 7918
BtgI C'CryG_G
Cuts at: 513 1065 2113 513
Size: 552 1048 6318
Fragments arranged by size:
6318 1048 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7179
Fragments arranged by size:
7179 683 56
BtsI GCAGTG_nn'
Cuts at: 5434 6228 6248 5434
Size: 794 20 7104
Fragments arranged by size:
7104 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3648 3683 529
Size: 35 3084 35 4764
Fragments arranged by size:
4764 3084 35 35
DraI TTT'AAA
Cuts at: 2163 2586 2904 3103 4272 5222 2163
Size: 423 318 199 1169 950 4859
Fragments arranged by size:
4859 1169 950 423 318 199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6925 809
Size: 507 5609 1802
Fragments arranged by size:
5609 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2441 4416 5365
Size: 54 1425 50 275 18 611 1975 949
Cuts at: 5365 6254 7883 8
Size: 889 1629 43
Fragments arranged by size:
1975 1629 1425 949 889 611 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5365 1537
Size: 275 3553 4090
Fragments arranged by size:
4090 3553 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3528 5106 5139 5848 1353
Size: 2175 1578 33 709 3423
Fragments arranged by size:
3423 2175 1578 709 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6505 6879 7025 387
Size: 1064 138 4916 374 146 1280
Fragments arranged by size:
4916 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3674 3674
Size: 7918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2819 3099 3710 3863 4544 4757 4910
Size: 393 1187 280 611 153 681 213 153
Cuts at: 4910 5981 6578 7365 7633 1239
Size: 1071 597 787 268 1524
Fragments arranged by size:
1524 1187 1071 787 681 611 597 393
280 268 213 153 153
EcoNI CCTnn'n_nnAGG
Cuts at: 5132 5132
Size: 7918
EcoO109I rG'GnC_Cy
Cuts at: 3052 3052
Size: 7918
EcoRI G'AATT_C
Cuts at: 2606 3332 3716 5227 5605 2606
Size: 726 384 1511 378 4919
Fragments arranged by size:
4919 1511 726 384 378
EcoRV GAT'ATC
Cuts at: 2077 2077
Size: 7918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4284 4975 4284
Size: 691 7227
FspI TGC'GCA
Cuts at: 6424 6424
Size: 7918
HaeII r_GCGC'y
Cuts at: 1678 7065 1678
Size: 5387 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3015 3047 4732 4764 6559
Size: 32 135 32 1443 32 1685 32 1795
Cuts at: 6559 6591 6633 6665 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1795 1685 1443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2194 3197 3703 4429 4909 5262
Size: 988 281 256 1003 506 726 480 353
Cuts at: 5262 5508 669
Size: 246 3079
Fragments arranged by size:
3079 1003 988 726 506 480 353 281
256 246
HindIII A'AGCT_T
Cuts at: 748 3440 748
Size: 2692 5226
HpaI GTT'AAC
Cuts at: 3703 4909 5508 3703
Size: 1206 599 6113
Fragments arranged by size:
6113 1206 599
KpnI G_GTAC'C
Cuts at: 5243 5243
Size: 7918
MfeI C'AATT_G
Cuts at: 5517 5517
Size: 7918
MluI A'CGCG_T
Cuts at: 7794 7794
Size: 7918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2446 3796 4090 4326 7032 7216 1305
Size: 1141 1350 294 236 2706 184 2007
Fragments arranged by size:
2706 2007 1350 1141 294 236 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2443 4418 10
Size: 54 1425 343 611 1975 3510
Fragments arranged by size:
3510 1975 1425 611 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 3204 5876 6235 6394 518
Size: 648 1009 1029 2672 359 159 2042
Fragments arranged by size:
2672 2042 1029 1009 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 4385 4454 5334 6011 7159 7404 1606
Size: 444 2335 69 880 677 1148 245 2120
Fragments arranged by size:
2335 2120 1148 880 677 444 245 69
NcoI C'CATG_G
Cuts at: 513 1065 2113 513
Size: 552 1048 6318
Fragments arranged by size:
6318 1048 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7918
NotI GC'GGCC_GC
Cuts at: 5365 5365
Size: 7918
NsiI A_TGCA'T
Cuts at: 3531 3726 4830 4834 3531
Size: 195 1104 4 6615
Fragments arranged by size:
6615 1104 195 4
NspI r_CATG'y
Cuts at: 1132 1229 2599 2675 3979 4832 5276 6821 1132
Size: 97 1370 76 1304 853 444 1545 2229
Fragments arranged by size:
2229 1545 1370 1304 853 444 97 76
PasI CC'CwG_GG
Cuts at: 1348 2716 1348
Size: 1368 6550
PciI A'CATG_T
Cuts at: 3975 6817 3975
Size: 2842 5076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2071 2439 3751 4641 1108
Size: 963 368 1312 890 4385
Fragments arranged by size:
4385 1312 963 890 368
PfoI T'CCnGG_A
Cuts at: 3744 4894 3744
Size: 1150 6768
PmeI GTTT'AAAC
Cuts at: 5222 5222
Size: 7918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5987 6019 7500 7532 5987
Size: 32 1481 32 6373
Fragments arranged by size:
6373 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2189 1613
Size: 576 7342
PsiI TTA'TAA
Cuts at: 2760 3566 5488 2760
Size: 806 1922 5190
Fragments arranged by size:
5190 1922 806
PstI C_TGCA'G
Cuts at: 830 4195 4735 5270 830
Size: 3365 540 535 3478
Fragments arranged by size:
3478 3365 540 535
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7918
PvuII CAG'CTG
Cuts at: 2050 4385 4454 2050
Size: 2335 69 5514
Fragments arranged by size:
5514 2335 69
SacI G_AGCT'C
Cuts at: 3676 3676
Size: 7918
SalI G'TCGA_C
Cuts at: 1936 2192 5260 1936
Size: 256 3068 4594
Fragments arranged by size:
4594 3068 256
SbfI CC_TGCA'GG
Cuts at: 5270 5270
Size: 7918
ScaI AGT'ACT
Cuts at: 2151 4411 6166 7878 2151
Size: 2260 1755 1712 2191
Fragments arranged by size:
2260 2191 1755 1712
SfcI C'TryA_G
Cuts at: 826 1045 3145 3696 4191 4731 5266 6401 7082
Size: 219 2100 551 495 540 535 1135 681
Cuts at: 7082 7273 826
Size: 191 1471
Fragments arranged by size:
2100 1471 1135 681 551 540 535 495
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7918
SmaI CCC'GGG
Cuts at: 1872 5245 1872
Size: 3373 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2142 2524 2993 4949 4980 6022
Size: 197 926 199 382 469 1956 31 1042
Cuts at: 6022 6923 7185 820
Size: 901 262 1553
Fragments arranged by size:
1956 1553 1042 926 901 469 382 262
199 197 31
SnaBI TAC'GTA
Cuts at: 493 5709 493
Size: 5216 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7918
SphI G_CATG'C
Cuts at: 1132 4832 5276 1132
Size: 3700 444 3774
Fragments arranged by size:
3774 3700 444
SspI AAT'ATT
Cuts at: 5 52 2914 4186 4470 5842 5
Size: 47 2862 1272 284 1372 2081
Fragments arranged by size:
2862 2081 1372 1272 284 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7918
StyI C'CwwG_G
Cuts at: 513 1065 2113 2288 2679 5357 513
Size: 552 1048 175 391 2678 3074
Fragments arranged by size:
3074 2678 1048 552 391 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4279 5945 5962 6115 6300 6745 325
Size: 1255 2699 1666 17 153 185 445 1498
Fragments arranged by size:
2699 1666 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2149 2668 3639
Size: 274 80 33 51 1443 172 519 971
Cuts at: 3639 3949 3972 4409 4880 6164 7876 96
Size: 310 23 437 471 1284 1712 138
Fragments arranged by size:
1712 1443 1284 971 519 471 437 310
274 172 138 80 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3309 3341 7878 7910 3309
Size: 32 4537 32 3317
Fragments arranged by size:
4537 3317 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7918
XbaI T'CTAG_A
Cuts at: 5254 5254
Size: 7918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7918
XmaI C'CCGG_G
Cuts at: 1870 5243 1870
Size: 3373 4545
XmnI GAAnn'nnTTC
Cuts at: 4547 6047 4547
Size: 1500 6418
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2343 5726 276
Size: 53 83 186 936 809 3383 2468
Fragments arranged by size:
3383 2468 936 809 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI