Restriction map for
pFN21AB0166
(Circular) (Six-base) MAPSORT of: pFN21AB0166.seq Check: 6877 from: 1 to: 8173
pFN21AB0166 8173 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 5983 278
Size: 53 83 186 936 2338 2109 2468
Fragments arranged by size:
2468 2338 2109 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3702 5516 1937
Size: 1765 1814 4594
Fragments arranged by size:
4594 1814 1765
Acc65I G'GTAC_C
Cuts at: 2039 5494 2039
Size: 3455 4718
AclI AA'CG_TT
Cuts at: 1563 2252 6300 6673 1563
Size: 689 4048 373 3063
Fragments arranged by size:
4048 3063 689 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2322 2414 2895 2972 3222 3465 4191 4382
Size: 690 92 481 77 250 243 726 191
Cuts at: 4382 4929 5222 6236 7620 7888 1632
Size: 547 293 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1014 726 690 547 481 293
268 250 243 191 92 77
AfeI AGC'GCT
Cuts at: 4664 5003 4664
Size: 339 7834
AflII C'TTAA_G
Cuts at: 820 1017 4463 820
Size: 197 3446 4530
Fragments arranged by size:
4530 3446 197
AflIII A'CryG_T
Cuts at: 1203 7072 8049 1203
Size: 5869 977 1327
Fragments arranged by size:
5869 1327 977
AgeI A'CCGG_T
Cuts at: 7004 7004
Size: 8173
AhdI GACnn_n'nnGTC
Cuts at: 6902 6902
Size: 8173
AleI CACnn'nnGTG
Cuts at: 1166 2457 1166
Size: 1291 6882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3959 3991 3959
Size: 32 8141
AlwNI CAG_nnn'CTG
Cuts at: 3164 3299 3488 3728 4573 5041 5916 7488 3164
Size: 135 189 240 845 468 875 1572 3849
Fragments arranged by size:
3849 1572 875 845 468 240 189 135
ApaI G_GGCC'C
Cuts at: 2060 2079 2060
Size: 19 8154
ApaLI G'TGCA_C
Cuts at: 6230 7386 6230
Size: 1156 7017
ApoI r'AATT_y
Cuts at: 1493 2796 3305 4690 4783 5482 5708 5860 7969
Size: 1303 509 1385 93 699 226 152 2109
Cuts at: 7969 1493
Size: 1697
Fragments arranged by size:
2109 1697 1385 1303 699 509 226 152
93
AseI AT'TA_AT
Cuts at: 160 1032 4827 6727 160
Size: 872 3795 1900 1606
Fragments arranged by size:
3795 1900 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8173
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2072 5498 1870
Size: 73 92 37 3426 4545
Fragments arranged by size:
4545 3426 92 73 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4613 4646 4613
Size: 33 8140
BamHI G'GATC_C
Cuts at: 5503 5870 5503
Size: 367 7806
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 3368 5494 6949 618
Size: 325 217 627 252 1329 2126 1455 1842
Fragments arranged by size:
2126 1842 1455 1329 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2060 2079 2714 2950 3636 5492 1391
Size: 669 19 635 236 686 1856 4072
Fragments arranged by size:
4072 1856 686 669 635 236 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2664 3210 3462 3816 928
Size: 971 765 546 252 354 5285
Fragments arranged by size:
5285 971 765 546 354 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6364 6398 1903
Size: 34 4427 34 3678
Fragments arranged by size:
4427 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4093 6065 7275 4093
Size: 1972 1210 4991
Fragments arranged by size:
4991 1972 1210
BclI T'GATC_A
Cuts at: 1548 4179 4684 1548
Size: 2631 505 5037
Fragments arranged by size:
5037 2631 505
BfrBI ATG'CAT
Cuts at: 2924 2924
Size: 8173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2746 6784 136
Size: 107 122 71 631 605 1074 4038 1525
Fragments arranged by size:
4038 1525 1074 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4537 5313 8168 4537
Size: 776 2855 4542
Fragments arranged by size:
4542 2855 776
BlpI GC'TnA_GC
Cuts at: 2177 4992 5590 2177
Size: 2815 598 4760
Fragments arranged by size:
4760 2815 598
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2079 6234 7390 1790
Size: 270 19 4155 1156 2573
Fragments arranged by size:
4155 2573 1156 270 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2135 3309 4669
Size: 416 53 83 399 24 718 1174 1360
Cuts at: 4669 5904 6862 442
Size: 1235 958 1753
Fragments arranged by size:
1753 1360 1235 1174 958 718 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2103 1056
Size: 1047 7126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2476 4136 4529 4643 4742 6833 1239
Size: 1237 1660 393 114 99 2091 2579
Fragments arranged by size:
2579 2091 1660 1237 393 114 99
Bpu10I CC'TnA_GC
Cuts at: 2713 2713
Size: 8173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3947 4156 4184 4942 6298 7163 7461 3947
Size: 209 28 758 1356 865 298 4659
Fragments arranged by size:
4659 1356 865 758 298 209 28
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3512 5015 6836 882
Size: 617 2013 1503 1821 2219
Fragments arranged by size:
2219 2013 1821 1503 617
BsaAI yAC'GTr
Cuts at: 493 1204 5964 493
Size: 711 4760 2702
Fragments arranged by size:
4760 2702 711
BsaBI GATnn'nnATC
Cuts at: 8167 8167
Size: 8173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 5980 6362 6997
Size: 53 83 186 936 2338 2109 382 635
Cuts at: 6997 275
Size: 1451
Fragments arranged by size:
2338 2109 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5362 6606 7004 7278 7425 7836 5362
Size: 1244 398 274 147 411 5699
Fragments arranged by size:
5699 1244 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3339 3369 3959 3989 1178
Size: 30 165 30 1936 30 590 30 5362
Fragments arranged by size:
5362 1936 590 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4139 4539 1186
Size: 2953 400 4820
Fragments arranged by size:
4820 2953 400
BseYI C'CCAG_C
Cuts at: 2011 3721 7376 7872 2011
Size: 1710 3655 496 2312
Fragments arranged by size:
3655 2312 1710 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2669 5070 1163
Size: 573 933 2401 4266
Fragments arranged by size:
4266 2401 933 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2160 2164 5623 6384 7412 1540
Size: 275 186 159 4 3459 761 1028 2301
Fragments arranged by size:
3459 2301 1028 761 275 186 159 4
BsiHKAI G_wGCw'C
Cuts at: 2148 2714 3290 3636 6234 6319 7390 2148
Size: 566 576 346 2598 85 1071 2931
Fragments arranged by size:
2931 2598 1071 576 566 346 85
BsmI GAATG_Cn'
Cuts at: 5340 5684 5777 5340
Size: 344 93 7736
Fragments arranged by size:
7736 344 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2079 2148 2714 2950 3290 3636
Size: 399 270 19 69 566 236 340 346
Cuts at: 3636 5492 6234 6319 7390 1391
Size: 1856 742 85 1071 2174
Fragments arranged by size:
2174 1856 1071 742 566 399 346 340
270 236 85 69 19
BspEI T'CCGG_A
Cuts at: 7836 7836
Size: 8173
BspHI T'CATG_A
Cuts at: 4798 6062 7792 4798
Size: 1264 1730 5179
Fragments arranged by size:
5179 1730 1264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5385 5528 844
Size: 4541 143 3489
Fragments arranged by size:
4541 3489 143
BsrBI CCG'CTC
Cuts at: 1394 1823 6060 1394
Size: 429 4237 3507
Fragments arranged by size:
4237 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6668 6842 65
Size: 1183 5420 174 1396
Fragments arranged by size:
5420 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 2163 6817 7004 1700
Size: 110 345 8 4654 187 2869
Fragments arranged by size:
4654 2869 345 187 110 8
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8173
BssSI C'ACGA_G
Cuts at: 6233 7245 6233
Size: 1012 7161
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2737 2755 794
Size: 1943 18 6212
Fragments arranged by size:
6212 1943 18
BstBI TT'CG_AA
Cuts at: 6977 6977
Size: 8173
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8173
BstYI r'GATC_y
Cuts at: 1972 2258 2549 2981 4537 5313 5503 5870 6255
Size: 286 291 432 1556 776 190 367 385
Cuts at: 6255 6272 7724 7810 7822 8168 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1556 1452 776 432 385 367 346
291 286 190 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2494 2742 3756 2494
Size: 248 1014 6911
Fragments arranged by size:
6911 1014 248
BtgI C'CryG_G
Cuts at: 513 1065 2027 3734 4295 513
Size: 552 962 1707 561 4391
Fragments arranged by size:
4391 1707 962 561 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5084 487
Size: 683 56 3858 3576
Fragments arranged by size:
3858 3576 683 56
BtsI GCAGTG_nn'
Cuts at: 2652 5689 6483 6503 2652
Size: 3037 794 20 4322
Fragments arranged by size:
4322 3037 794 20
ClaI AT'CG_AT
Cuts at: 1554 4431 1554
Size: 2877 5296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8138
DraI TTT'AAA
Cuts at: 5331 5477 5331
Size: 146 8027
DraIII CAC_nnn'GTG
Cuts at: 2444 2444
Size: 8173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7180 809
Size: 507 5864 1802
Fragments arranged by size:
5864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2161 2564
Size: 54 1425 50 275 18 234 97 403
Cuts at: 2564 3906 5620 6509 8138 8
Size: 1342 1714 889 1629 43
Fragments arranged by size:
1714 1629 1425 1342 889 403 275 234
97 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2161 5620 1537
Size: 275 349 3459 4090
Fragments arranged by size:
4090 3459 349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2271 2491 3318 4252 4557 4712 5122
Size: 800 118 220 827 934 305 155 410
Cuts at: 5122 5241 6103 1353
Size: 119 862 3423
Fragments arranged by size:
3423 934 862 827 800 410 305 220
155 119 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5060 6760 7134 7280 387
Size: 1064 138 3471 1700 374 146 1280
Fragments arranged by size:
3471 1700 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2712 3634 2712
Size: 922 7251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2322 2414 2476 2895 2972 3222 3465
Size: 393 690 92 62 419 77 250 243
Cuts at: 3465 4136 4191 4382 4529 4643 4742 4929 5222
Size: 671 55 191 147 114 99 187 293
Cuts at: 5222 6236 6833 7620 7888 1239
Size: 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 787 690 671 597 419 393
293 268 250 243 191 187 147 114
99 92 77 62 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2741 2741
Size: 8173
EcoO109I rG'GnC_Cy
Cuts at: 2046 2056 2046
Size: 10 8163
EcoRI G'AATT_C
Cuts at: 5482 5860 5482
Size: 378 7795
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2774 2774
Size: 8173
FspI TGC'GCA
Cuts at: 6679 6679
Size: 8173
HaeII r_GCGC'y
Cuts at: 1678 4666 5005 7320 1678
Size: 2988 339 2315 2531
Fragments arranged by size:
2988 2531 2315 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3202 3234 3241 3273 3808
Size: 32 135 32 1630 32 7 32 535
Cuts at: 3808 3840 3895 3906 3927 3938 4524 4529 4556
Size: 32 55 11 21 11 586 5 27
Cuts at: 4556 4561 5237 5269 6814 6846 6888 6920 1373
Size: 5 676 32 1545 32 42 32 2626
Fragments arranged by size:
2626 1630 1545 676 586 535 135 55
42 32 32 32 32 32 32 32
32 27 21 11 11 7 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3019 3225 3997 5517 5763 669
Size: 988 281 1081 206 772 1520 246 3079
Fragments arranged by size:
3079 1520 1081 988 772 281 246 206
HindIII A'AGCT_T
Cuts at: 748 2857 5154 5401 748
Size: 2109 2297 247 3520
Fragments arranged by size:
3520 2297 2109 247
HpaI GTT'AAC
Cuts at: 5763 5763
Size: 8173
KpnI G_GTAC'C
Cuts at: 2043 5498 2043
Size: 3455 4718
MfeI C'AATT_G
Cuts at: 5280 5772 5280
Size: 492 7681
MluI A'CGCG_T
Cuts at: 8049 8049
Size: 8173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4071 5148 7287 7471 1305
Size: 2766 1077 2139 184 2007
Fragments arranged by size:
2766 2139 2007 1077 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2566 3908 10
Size: 54 1425 343 734 1342 4275
Fragments arranged by size:
4275 1425 1342 734 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2457 6131 6490 6649 518
Size: 648 1291 3674 359 159 2042
Fragments arranged by size:
3674 2042 1291 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2062 2317 2434 4417 4812 5038 5589
Size: 423 33 255 117 1983 395 226 551
Cuts at: 5589 6266 7414 7659 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1983 1148 677 551 423 395 255
245 226 117 33
NaeI GCC'GGC
Cuts at: 2165 2165
Size: 8173
NcoI C'CATG_G
Cuts at: 513 1065 4295 513
Size: 552 3230 4391
Fragments arranged by size:
4391 3230 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8173
NgoMIV G'CCGG_C
Cuts at: 2163 2163
Size: 8173
NheI G'CTAG_C
Cuts at: 1052 2099 1052
Size: 1047 7126
NotI GC'GGCC_GC
Cuts at: 5620 5620
Size: 8173
NsiI A_TGCA'T
Cuts at: 2926 2926
Size: 8173
NspI r_CATG'y
Cuts at: 1132 1229 2758 2924 3935 5531 7076 1132
Size: 97 1529 166 1011 1596 1545 2229
Fragments arranged by size:
2229 1596 1545 1529 1011 166 97
PasI CC'CwG_GG
Cuts at: 1348 2119 1348
Size: 771 7402
PciI A'CATG_T
Cuts at: 7072 7072
Size: 8173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4164 1108
Size: 3056 5117
PfoI T'CCnGG_A
Cuts at: 4440 4440
Size: 8173
PmeI GTTT'AAAC
Cuts at: 5477 5477
Size: 8173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 6242 6274 7755 7787 3959
Size: 32 2251 32 1481 32 4345
Fragments arranged by size:
4345 2251 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2046
Size: 8173
PshAI GACnn'nnGTC
Cuts at: 1613 3415 1613
Size: 1802 6371
PsiI TTA'TAA
Cuts at: 5743 5743
Size: 8173
PspOMI G'GGCC_C
Cuts at: 2056 2075 2056
Size: 19 8154
PstI C_TGCA'G
Cuts at: 830 2131 3596 4063 4215 5525 830
Size: 1301 1465 467 152 1310 3478
Fragments arranged by size:
3478 1465 1310 1301 467 152
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8173
PvuII CAG'CTG
Cuts at: 2317 2434 4417 4812 5038 2317
Size: 117 1983 395 226 5452
Fragments arranged by size:
5452 1983 395 226 117
SacI G_AGCT'C
Cuts at: 2714 3636 2714
Size: 922 7251
SacII CC_GC'GG
Cuts at: 2030 2030
Size: 8173
SalI G'TCGA_C
Cuts at: 1936 5515 1936
Size: 3579 4594
SanDI GG'GwC_CC
Cuts at: 2046 2046
Size: 8173
SapI GCTCTTCn'nnn_
Cuts at: 2153 5122 5241 2153
Size: 2969 119 5085
Fragments arranged by size:
5085 2969 119
SbfI CC_TGCA'GG
Cuts at: 5525 5525
Size: 8173
ScaI AGT'ACT
Cuts at: 5357 6421 8133 5357
Size: 1064 1712 5397
Fragments arranged by size:
5397 1712 1064
SfcI C'TryA_G
Cuts at: 826 1045 2127 3165 3540 3592 4059 4211 4523
Size: 219 1082 1038 375 52 467 152 312
Cuts at: 4523 5521 6656 7337 7528 826
Size: 998 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 1038 998 681 467 375
312 219 191 152 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8173
SmaI CCC'GGG
Cuts at: 1872 2037 5500 1872
Size: 165 3463 4545
Fragments arranged by size:
4545 3463 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3962 4135 4199 4463 4921 6277
Size: 197 926 2019 173 64 264 458 1356
Cuts at: 6277 7178 7440 820
Size: 901 262 1553
Fragments arranged by size:
2019 1553 1356 926 901 458 264 262
197 173 64
SnaBI TAC'GTA
Cuts at: 493 5964 493
Size: 5471 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8173
SphI G_CATG'C
Cuts at: 1132 2758 2924 3935 5531 1132
Size: 1626 166 1011 1596 3774
Fragments arranged by size:
3774 1626 1596 1011 166
SspI AAT'ATT
Cuts at: 5 52 4195 5453 6097 5
Size: 47 4143 1258 644 2081
Fragments arranged by size:
4143 2081 1258 644 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8173
StyI C'CwwG_G
Cuts at: 513 1065 4295 5612 513
Size: 552 3230 1317 3074
Fragments arranged by size:
3230 3074 1317 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4424 6200 6217 6370 6555 7000 325
Size: 1255 2844 1776 17 153 185 445 1498
Fragments arranged by size:
2844 1776 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5355 6419 8131
Size: 274 80 33 51 1443 3378 1064 1712
Cuts at: 8131 96
Size: 138
Fragments arranged by size:
3378 1712 1443 1064 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2190 2222 3777 3809 8133 8165 2190
Size: 32 1555 32 4324 32 2198
Fragments arranged by size:
4324 2198 1555 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3992 1316
Size: 2676 5497
XbaI T'CTAG_A
Cuts at: 3398 5509 3398
Size: 2111 6062
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8173
XmaI C'CCGG_G
Cuts at: 1870 2035 5498 1870
Size: 165 3463 4545
Fragments arranged by size:
4545 3463 165
XmnI GAAnn'nnTTC
Cuts at: 2779 4290 6302 2779
Size: 1511 2012 4650
Fragments arranged by size:
4650 2012 1511
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 5981 276
Size: 53 83 186 936 2338 2109 2468
Fragments arranged by size:
2468 2338 2109 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI
BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PspXI PsrI RsrII SexAI SfiI SfoI
SrfI SwaI XcmI