Restriction map for
pFN21AB0100
(Circular) (Six-base) MAPSORT of: pFN21AB0100.seq Check: 6384 from: 1 to: 7768
pFN21AB0100 7768 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2381 2705 2381
Size: 324 7444
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5578 278
Size: 53 83 186 936 4042 2468
Fragments arranged by size:
4042 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5111 1937
Size: 3174 4594
Acc65I G'GTAC_C
Cuts at: 5089 5089
Size: 7768
AclI AA'CG_TT
Cuts at: 1563 5895 6268 1563
Size: 4332 373 3063
Fragments arranged by size:
4332 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2070 4918 5015 5831 7215 7483 1632
Size: 402 36 2848 97 816 1384 268 1917
Fragments arranged by size:
2848 1917 1384 816 402 268 97 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7571
AflIII A'CryG_T
Cuts at: 1203 3361 6667 7644 1203
Size: 2158 3306 977 1327
Fragments arranged by size:
3306 2158 1327 977
AgeI A'CCGG_T
Cuts at: 6599 6599
Size: 7768
AhdI GACnn_n'nnGTC
Cuts at: 6497 6497
Size: 7768
AleI CACnn'nnGTG
Cuts at: 1166 2789 1166
Size: 1623 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7768
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4286 4318 4286
Size: 32 7736
AlwNI CAG_nnn'CTG
Cuts at: 4114 4360 5511 7083 4114
Size: 246 1151 1572 4799
Fragments arranged by size:
4799 1572 1151 246
ApaI G_GGCC'C
Cuts at: 2245 2305 3691 4466 2245
Size: 60 1386 775 5547
Fragments arranged by size:
5547 1386 775 60
ApaLI G'TGCA_C
Cuts at: 3485 5825 6981 3485
Size: 2340 1156 4272
Fragments arranged by size:
4272 2340 1156
ApoI r'AATT_y
Cuts at: 1493 5077 5303 5455 7564 1493
Size: 3584 226 152 2109 1697
Fragments arranged by size:
3584 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6322 160
Size: 872 5290 1606
Fragments arranged by size:
5290 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7768
AvaI C'yCGr_G
Cuts at: 1870 1943 3180 3923 3990 5093 1870
Size: 73 1237 743 67 1103 4545
Fragments arranged by size:
4545 1237 1103 743 73 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4541 4574 4541
Size: 33 7735
BamHI G'GATC_C
Cuts at: 5098 5465 5098
Size: 367 7401
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2369 2445 2783 3171
Size: 325 217 627 465 117 76 338 388
Cuts at: 3171 4127 5089 6544 618
Size: 956 962 1455 1842
Fragments arranged by size:
1842 1455 962 956 627 465 388 338
325 217 117 76
BanII G_rGCy'C
Cuts at: 1391 2245 2305 2418 2967 3411 3659 3691 4466
Size: 854 60 113 549 444 248 32 775
Cuts at: 4466 5057 5087 1391
Size: 591 30 4072
Fragments arranged by size:
4072 854 775 591 549 444 248 113
60 32 30
BbeI G_GCGC'C
Cuts at: 3175 4131 3175
Size: 956 6812
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2076 2967 3600 3753 3850 4968 928
Size: 971 177 891 633 153 97 1118 3728
Fragments arranged by size:
3728 1118 971 891 633 177 153 97
BbvCI CC'TCA_GC
Cuts at: 2409 2409
Size: 7768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5959 5993 1903
Size: 34 4022 34 3678
Fragments arranged by size:
4022 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2909 4542 5660 6870 2909
Size: 1633 1118 1210 3807
Fragments arranged by size:
3807 1633 1210 1118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7768
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 2663 2750
Size: 107 122 71 631 605 637 354 87
Cuts at: 2750 3060 3318 4109 4118 6379 136
Size: 310 258 791 9 2261 1525
Fragments arranged by size:
2261 1525 791 637 631 605 354 310
258 122 107 87 71 9
BglII A'GATC_T
Cuts at: 7763 7763
Size: 7768
BlpI GC'TnA_GC
Cuts at: 3151 3410 3653 4947 4952 5185 3151
Size: 259 243 1294 5 233 5734
Fragments arranged by size:
5734 1294 259 243 233 5
Bme1580I G_kGCm'C
Cuts at: 1790 2245 2305 2786 3272 3361 3468 3489 3691
Size: 455 60 481 486 89 107 21 202
Cuts at: 3691 4466 4714 5829 6985 1790
Size: 775 248 1115 1156 2573
Fragments arranged by size:
2573 1156 1115 775 486 481 455 248
202 107 89 60 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7768
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2020 4600 5499
Size: 416 53 83 399 24 603 2580 899
Cuts at: 5499 6457 442
Size: 958 1753
Fragments arranged by size:
2580 1753 958 899 603 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2332 3077 4226 4558 4638 4910 6428
Size: 993 100 745 1149 332 80 272 1518
Cuts at: 6428 1239
Size: 2579
Fragments arranged by size:
2579 1518 1149 993 745 332 272 100
80
Bpu10I CC'TnA_GC
Cuts at: 2409 2409
Size: 7768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3732 5893 6758 7056 3732
Size: 2161 865 298 4444
Fragments arranged by size:
4444 2161 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 3620 6431 882
Size: 617 876 1245 2811 2219
Fragments arranged by size:
2811 2219 1245 876 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3364 5559 493
Size: 711 1431 729 2195 2702
Fragments arranged by size:
2702 2195 1431 729 711
BsaBI GATnn'nnATC
Cuts at: 7762 7762
Size: 7768
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2203 3172 4128 5575
Size: 53 83 186 936 670 969 956 1447
Cuts at: 5575 5957 6592 275
Size: 382 635 1451
Fragments arranged by size:
1451 1447 969 956 936 670 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2123 3772 6201 6599 6873 7020 7431 2123
Size: 1649 2429 398 274 147 411 2460
Fragments arranged by size:
2460 2429 1649 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2861 2891 3094 3119 3124
Size: 30 165 30 1458 30 203 25 5
Cuts at: 3124 3149 4286 4316 4895 4925 1178
Size: 25 1137 30 579 30 4021
Fragments arranged by size:
4021 1458 1137 579 203 165 30 30
30 30 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2491 2494 2497 2500 2503 2506 2509
Size: 1240 65 3 3 3 3 3 3
Cuts at: 2509 2825 2946 3414 4037 1186
Size: 316 121 468 623 4917
Fragments arranged by size:
4917 1240 623 468 316 121 65 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2561 2753 2981 3031 3187 3765 4171 4482 6971
Size: 192 228 50 156 578 406 311 2489
Cuts at: 6971 7467 2561
Size: 496 2862
Fragments arranged by size:
2862 2489 578 496 406 311 228 192
156 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2840 3085 3404 4997 1163
Size: 573 1104 245 319 1593 3934
Fragments arranged by size:
3934 1593 1104 573 319 245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3855 5218 5979 7007 1540
Size: 275 186 1854 1363 761 1028 2301
Fragments arranged by size:
2301 1854 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2712 3411 3489 5829 5914 6985 2712
Size: 699 78 2340 85 1071 3495
Fragments arranged by size:
3495 2340 1071 699 85 78
BsmI GAATG_Cn'
Cuts at: 2992 5279 5372 2992
Size: 2287 93 5388
Fragments arranged by size:
5388 2287 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2729 2729
Size: 7768
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2245 2305 2418 2712 2786 2967 3272
Size: 399 455 60 113 294 74 181 305
Cuts at: 3272 3361 3411 3468 3489 3659 3691 4466 4714
Size: 89 50 57 21 170 32 775 248
Cuts at: 4714 5057 5087 5829 5914 6985 1391
Size: 343 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 775 742 455 399 343 305
294 248 181 170 113 89 85 74
60 57 50 32 30 21
BspEI T'CCGG_A
Cuts at: 3772 7431 3772
Size: 3659 4109
BspHI T'CATG_A
Cuts at: 5657 7387 5657
Size: 1730 6038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 2705 3213 3259 4974 5123 844
Size: 1537 324 508 46 1715 149 3489
Fragments arranged by size:
3489 1715 1537 508 324 149 46
BsrBI CCG'CTC
Cuts at: 1394 1823 2293 2364 2599 5655 1394
Size: 429 470 71 235 3056 3507
Fragments arranged by size:
3507 3056 470 429 235 71
BsrDI GCAATG_nn'
Cuts at: 65 1248 4500 6263 6437 65
Size: 1183 3252 1763 174 1396
Fragments arranged by size:
3252 1763 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3701 6412 6599 1700
Size: 110 1891 2711 187 2869
Fragments arranged by size:
2869 2711 1891 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7768
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7768
BssSI C'ACGA_G
Cuts at: 2928 5828 6840 2928
Size: 2900 1012 3856
Fragments arranged by size:
3856 2900 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7768
BstBI TT'CG_AA
Cuts at: 6572 6572
Size: 7768
BstEII G'GTnAC_C
Cuts at: 1184 4216 1184
Size: 3032 4736
BstXI CCAn_nnnn'nTGG
Cuts at: 2169 2561 3548 3671 4888 2169
Size: 392 987 123 1217 5049
Fragments arranged by size:
5049 1217 987 392 123
BstYI r'GATC_y
Cuts at: 1972 5098 5465 5850 5867 7319 7405 7417 7763
Size: 3126 367 385 17 1452 86 12 346
Cuts at: 7763 1972
Size: 1977
Fragments arranged by size:
3126 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3426 4283 4458 5042 3426
Size: 857 175 584 6152
Fragments arranged by size:
6152 857 584 175
BtgI C'CryG_G
Cuts at: 513 1065 2004 2554 2957 3706 4076 513
Size: 552 939 550 403 749 370 4205
Fragments arranged by size:
4205 939 749 552 550 403 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4367 487
Size: 683 56 3141 3888
Fragments arranged by size:
3888 3141 683 56
BtsI GCAGTG_nn'
Cuts at: 2857 3633 4173 5284 6078 6098 2857
Size: 776 540 1111 794 20 4527
Fragments arranged by size:
4527 1111 794 776 540 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7768
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7733
DraI TTT'AAA
Cuts at: 5072 5072
Size: 7768
DraIII CAC_nnn'GTG
Cuts at: 2287 3493 4405 4426 2287
Size: 1206 912 21 5629
Fragments arranged by size:
5629 1206 912 21
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6775 809
Size: 507 5459 1802
Fragments arranged by size:
5459 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3527 3661 3703
Size: 54 1425 50 275 18 1697 134 42
Cuts at: 3703 4101 4323 4447 4993 5215 6104 7733 8
Size: 398 222 124 546 222 889 1629 43
Fragments arranged by size:
1697 1629 1425 889 546 398 275 222
222 134 124 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 5215 1537
Size: 275 3403 4090
Fragments arranged by size:
4090 3403 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2527 2690 3215 5698 1353
Size: 744 430 163 525 2483 3423
Fragments arranged by size:
3423 2483 744 525 430 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6355 6729 6875 387
Size: 1064 138 4766 374 146 1280
Fragments arranged by size:
4766 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3409 3409
Size: 7768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2070 2232 2332 3077 4226 4558
Size: 393 402 36 162 100 745 1149 332
Cuts at: 4558 4638 4910 4918 5015 5831 6428 7215 7483
Size: 80 272 8 97 816 597 787 268
Cuts at: 7483 1239
Size: 1524
Fragments arranged by size:
1524 1149 816 787 745 597 402 393
332 272 268 162 100 97 80 36
8
EcoO109I rG'GnC_Cy
Cuts at: 2241 2242 2301 2549 2655 2760 3094 3202 3961
Size: 1 59 248 106 105 334 108 759
Cuts at: 3961 4111 4462 4792 4816 2241
Size: 150 351 330 24 5193
Fragments arranged by size:
5193 759 351 334 330 248 150 108
106 105 59 24 1
EcoRI G'AATT_C
Cuts at: 5077 5455 5077
Size: 378 7390
FspI TGC'GCA
Cuts at: 6274 6274
Size: 7768
HaeII r_GCGC'y
Cuts at: 1678 3175 4131 6915 1678
Size: 1497 956 2784 2531
Fragments arranged by size:
2784 2531 1497 956
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3730 3762 4895 4927 6409
Size: 32 135 32 2158 32 1133 32 1482
Cuts at: 6409 6441 6483 6515 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2158 1482 1133 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5112 5358 669
Size: 988 281 3174 246 3079
Fragments arranged by size:
3174 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7768
HpaI GTT'AAC
Cuts at: 5358 5358
Size: 7768
KasI G'GCGC_C
Cuts at: 3171 4127 3171
Size: 956 6812
KpnI G_GTAC'C
Cuts at: 5093 5093
Size: 7768
MfeI C'AATT_G
Cuts at: 2266 2475 5367 2266
Size: 209 2892 4667
Fragments arranged by size:
4667 2892 209
MluI A'CGCG_T
Cuts at: 7644 7644
Size: 7768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3105 3531 4668 6882 7066 1305
Size: 1800 426 1137 2214 184 2007
Fragments arranged by size:
2214 2007 1800 1137 426 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3663 4103 4325 4449 4995
Size: 54 1425 343 1831 440 222 124 546
Cuts at: 4995 10
Size: 2783
Fragments arranged by size:
2783 1831 1425 546 440 343 222 124
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 3518 3669 3842 5726 6085 6244
Size: 648 1623 729 151 173 1884 359 159
Cuts at: 6244 518
Size: 2042
Fragments arranged by size:
2042 1884 1623 729 648 359 173 159
151
MspA1I CmG'CkG
Cuts at: 1606 2569 2777 2904 3258 3282 3652 3865 3898
Size: 963 208 127 354 24 370 213 33
Cuts at: 3898 4291 4318 4333 4735 5184 5861 7009 7254
Size: 393 27 15 402 449 677 1148 245
Cuts at: 7254 1606
Size: 2120
Fragments arranged by size:
2120 1148 963 677 449 402 393 370
354 245 213 208 127 33 27 24
15
NaeI GCC'GGC
Cuts at: 3703 3703
Size: 7768
NarI GG'CG_CC
Cuts at: 3172 4128 3172
Size: 956 6812
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6277
Fragments arranged by size:
6277 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7768
NgoMIV G'CCGG_C
Cuts at: 3701 3701
Size: 7768
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7768
NotI GC'GGCC_GC
Cuts at: 5215 5215
Size: 7768
NspI r_CATG'y
Cuts at: 1132 1229 2229 2346 5126 6671 1132
Size: 97 1000 117 2780 1545 2229
Fragments arranged by size:
2780 2229 1545 1000 117 97
PasI CC'CwG_GG
Cuts at: 1348 4796 4975 1348
Size: 3448 179 4141
Fragments arranged by size:
4141 3448 179
PciI A'CATG_T
Cuts at: 6667 6667
Size: 7768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4738 1108
Size: 3630 4138
PfoI T'CCnGG_A
Cuts at: 2643 3558 4666 2643
Size: 915 1108 5745
Fragments arranged by size:
5745 1108 915
PmeI GTTT'AAAC
Cuts at: 5072 5072
Size: 7768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 4619 4651 5837 5869 7350 7382 4286
Size: 32 301 32 1186 32 1481 32 4672
Fragments arranged by size:
4672 1481 1186 301 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2549 3202 3961 4792 2549
Size: 653 759 831 5525
Fragments arranged by size:
5525 831 759 653
PshAI GACnn'nnGTC
Cuts at: 1613 3966 1613
Size: 2353 5415
PsiI TTA'TAA
Cuts at: 5338 5338
Size: 7768
PspOMI G'GGCC_C
Cuts at: 2241 2301 3687 4462 2241
Size: 60 1386 775 5547
Fragments arranged by size:
5547 1386 775 60
PstI C_TGCA'G
Cuts at: 830 2536 3287 3436 3903 4074 4143 4317 4782
Size: 1706 751 149 467 171 69 174 465
Cuts at: 4782 5120 830
Size: 338 3478
Fragments arranged by size:
3478 1706 751 467 465 338 174 171
149 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7768
PvuII CAG'CTG
Cuts at: 2777 3652 3898 4318 4735 2777
Size: 875 246 420 417 5810
Fragments arranged by size:
5810 875 420 417 246
SacI G_AGCT'C
Cuts at: 3411 3411
Size: 7768
SalI G'TCGA_C
Cuts at: 1936 5110 1936
Size: 3174 4594
SanDI GG'GwC_CC
Cuts at: 2549 4792 2549
Size: 2243 5525
SapI GCTCTTCn'nnn_
Cuts at: 2690 3215 2690
Size: 525 7243
SbfI CC_TGCA'GG
Cuts at: 4782 5120 4782
Size: 338 7430
ScaI AGT'ACT
Cuts at: 6016 7728 6016
Size: 1712 6056
SexAI A'CCwGG_T
Cuts at: 3489 3489
Size: 7768
SfcI C'TryA_G
Cuts at: 826 1045 2532 3283 3432 3899 4070 4139 4313
Size: 219 1487 751 149 467 171 69 174
Cuts at: 4313 4778 5116 6251 6932 7123 826
Size: 465 338 1135 681 191 1471
Fragments arranged by size:
1487 1471 1135 751 681 467 465 338
219 191 174 171 149 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 2663 4109 4118 2663
Size: 1446 9 6313
Fragments arranged by size:
6313 1446 9
SfoI GGC'GCC
Cuts at: 3173 4129 3173
Size: 956 6812
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7768
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7768
SmaI CCC'GGG
Cuts at: 1872 3992 5095 1872
Size: 2120 1103 4545
Fragments arranged by size:
4545 2120 1103
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3747 5872 6773 7035 820
Size: 197 926 1804 2125 901 262 1553
Fragments arranged by size:
2125 1804 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5559 493
Size: 5066 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7768
SphI G_CATG'C
Cuts at: 1132 2346 5126 1132
Size: 1214 2780 3774
Fragments arranged by size:
3774 2780 1214
SspI AAT'ATT
Cuts at: 5 52 5692 5
Size: 47 5640 2081
Fragments arranged by size:
5640 2081 47
StuI AGG'CCT
Cuts at: 1517 2942 3321 4807 4868 1517
Size: 1425 379 1486 61 4417
Fragments arranged by size:
4417 1486 1425 379 61
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 3786 5207 513
Size: 552 939 459 1323 1421 3074
Fragments arranged by size:
3074 1421 1323 939 552 459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2056 2345 2633 5795 5812 5965 6150
Size: 1255 476 289 288 3162 17 153 185
Cuts at: 6150 6595 325
Size: 445 1498
Fragments arranged by size:
3162 1498 1255 476 445 289 288 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6014 7726 96
Size: 274 80 33 51 1443 4037 1712 138
Fragments arranged by size:
4037 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2861 2893 3094 3126 3859 3891 3912 3944 7728
Size: 32 201 32 733 32 21 32 3784
Cuts at: 7728 7760 2861
Size: 32 2869
Fragments arranged by size:
3784 2869 733 201 32 32 32 32
32 21
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7768
XbaI T'CTAG_A
Cuts at: 5104 5104
Size: 7768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2166 3844 2166
Size: 1678 6090
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7768
XmaI C'CCGG_G
Cuts at: 1870 3990 5093 1870
Size: 2120 1103 4545
Fragments arranged by size:
4545 2120 1103
XmnI GAAnn'nnTTC
Cuts at: 5897 5897
Size: 7768
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5576 276
Size: 53 83 186 936 4042 2468
Fragments arranged by size:
4042 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SrfI SwaI