Restriction map for
pFN21AB0067
(Circular) (Six-base) MAPSORT of: pFN21AB0067.seq Check: 6897 from: 1 to: 12031
pFN21AB0067 12031 bp
With 182 enzymes: SgfI *
May 19, 2009 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6921 7130 8248 6921
Size: 209 1118 10704
Fragments arranged by size:
10704 1118 209
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5475 9841 278
Size: 53 83 186 936 3939 4366 2468
Fragments arranged by size:
4366 3939 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6954 7116 9374 1937
Size: 5017 162 2258 4594
Fragments arranged by size:
5017 4594 2258 162
Acc65I G'GTAC_C
Cuts at: 9352 9352
Size: 12031
AclI AA'CG_TT
Cuts at: 1563 3174 10158 10531 1563
Size: 1611 6984 373 3063
Fragments arranged by size:
6984 3063 1611 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2373 2940 5184 5898 6081 6606 8246 10094
Size: 741 567 2244 714 183 525 1640 1848
Cuts at: 10094 11478 11746 1632
Size: 1384 268 1917
Fragments arranged by size:
2244 1917 1848 1640 1384 741 714 567
525 268 183
AfeI AGC'GCT
Cuts at: 7325 8200 7325
Size: 875 11156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11834
AflIII A'CryG_T
Cuts at: 1203 3787 3921 6840 7347 10930 11907 1203
Size: 2584 134 2919 507 3583 977 1327
Fragments arranged by size:
3583 2919 2584 1327 977 507 134
AgeI A'CCGG_T
Cuts at: 3761 10862 3761
Size: 7101 4930
AhdI GACnn_n'nnGTC
Cuts at: 3195 5323 9167 10760 3195
Size: 2128 3844 1593 4466
Fragments arranged by size:
4466 3844 2128 1593
AleI CACnn'nnGTG
Cuts at: 1166 2879 2966 7799 1166
Size: 1713 87 4833 5398
Fragments arranged by size:
5398 4833 1713 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2303 3417 7737 8673 1184
Size: 1119 1114 4320 936 4542
Fragments arranged by size:
4542 4320 1119 1114 936
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 11999
AlwNI CAG_nnn'CTG
Cuts at: 2192 2299 3047 3076 3802 4384 4973 6865 7339
Size: 107 748 29 726 582 589 1892 474
Cuts at: 7339 7648 7918 8263 8428 8596 9047 9774 11346
Size: 309 270 345 165 168 451 727 1572
Cuts at: 11346 2192
Size: 2877
Fragments arranged by size:
2877 1892 1572 748 727 726 589 582
474 451 345 309 270 168 165 107
29
ApaI G_GGCC'C
Cuts at: 2433 8037 8157 2433
Size: 5604 120 6307
Fragments arranged by size:
6307 5604 120
ApaLI G'TGCA_C
Cuts at: 4335 4859 7343 10088 11244 4335
Size: 524 2484 2745 1156 5122
Fragments arranged by size:
5122 2745 2484 1156 524
ApoI r'AATT_y
Cuts at: 1493 3360 4168 5367 5972 6035 9340 9566 9718
Size: 1867 808 1199 605 63 3305 226 152
Cuts at: 9718 11827 1493
Size: 2109 1697
Fragments arranged by size:
3305 2109 1867 1697 1199 808 605 226
152 63
AseI AT'TA_AT
Cuts at: 160 1032 2598 10585 160
Size: 872 1566 7987 1606
Fragments arranged by size:
7987 1606 1566 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12031
AvaI C'yCGr_G
Cuts at: 1870 1943 2707 4066 5520 6122 7673 7975 8057
Size: 73 764 1359 1454 602 1551 302 82
Cuts at: 8057 9356 1870
Size: 1299 4545
Fragments arranged by size:
4545 1551 1454 1359 1299 764 602 302
82 73
BamHI G'GATC_C
Cuts at: 9361 9728 9361
Size: 367 11664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2702 4363 4474 6045 7828
Size: 325 217 627 915 1661 111 1571 1783
Cuts at: 7828 8074 9352 10807 618
Size: 246 1278 1455 1842
Fragments arranged by size:
1842 1783 1661 1571 1455 1278 915 627
325 246 217 111
BanII G_rGCy'C
Cuts at: 1391 2369 2433 4075 4277 5028 5055 5454 7295
Size: 978 64 1642 202 751 27 399 1841
Cuts at: 7295 8037 8157 8951 9350 1391
Size: 742 120 794 399 4072
Fragments arranged by size:
4072 1841 1642 978 794 751 742 399
399 202 120 64 27
BbeI G_GCGC'C
Cuts at: 2706 4367 6049 8078 2706
Size: 1661 1682 2029 6659
Fragments arranged by size:
6659 2029 1682 1661
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4677 5003 6027 6594 6889 928
Size: 971 2778 326 1024 567 295 6070
Fragments arranged by size:
6070 2778 1024 971 567 326 295
BbvCI CC'TCA_GC
Cuts at: 3725 4314 7638 7663 8244 3725
Size: 589 3324 25 581 7512
Fragments arranged by size:
7512 3324 589 581 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2698 2732 4840 4874 9203 9237 10222
Size: 34 761 34 2108 34 4329 34 985
Cuts at: 10222 10256 1903
Size: 34 3678
Fragments arranged by size:
4329 3678 2108 985 761 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9096 9923 11133 9096
Size: 827 1210 9994
Fragments arranged by size:
9994 1210 827
BclI T'GATC_A
Cuts at: 1548 6472 1548
Size: 4924 7107
BfrBI ATG'CAT
Cuts at: 5875 5875
Size: 12031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2304 4070 6496
Size: 107 122 71 631 605 632 1766 2426
Cuts at: 6496 8059 8141 8441 8666 10642 136
Size: 1563 82 300 225 1976 1525
Fragments arranged by size:
2426 1976 1766 1563 1525 632 631 605
300 225 122 107 82 71
BglII A'GATC_T
Cuts at: 12026 12026
Size: 12031
BlpI GC'TnA_GC
Cuts at: 4074 6971 7313 9448 4074
Size: 2897 342 2135 6657
Fragments arranged by size:
6657 2897 2135 342
Bme1580I G_kGCm'C
Cuts at: 1790 2433 4339 4725 4863 6414 7347 7527 8013
Size: 643 1906 386 138 1551 933 180 486
Cuts at: 8013 8037 8157 8688 10092 11248 1790
Size: 24 120 531 1404 1156 2573
Fragments arranged by size:
2573 1906 1551 1404 1156 933 643 531
486 386 180 138 120 24
BmgBI CAC'GTC
Cuts at: 1318 2508 6244 9015 9130 1318
Size: 1190 3736 2771 115 4219
Fragments arranged by size:
4219 3736 2771 1190 115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2823 2884 4719
Size: 416 53 83 399 24 1406 61 1835
Cuts at: 4719 4796 6014 7039 9061 9762 10720 442
Size: 77 1218 1025 2022 701 958 1753
Fragments arranged by size:
2022 1835 1753 1406 1218 1025 958 701
416 399 83 77 61 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 7057 2069
Size: 4988 7043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2673 2874 2898 3333 4527 4595 8351 8756
Size: 1434 201 24 435 1194 68 3756 405
Cuts at: 8756 9024 10691 1239
Size: 268 1667 2579
Fragments arranged by size:
3756 2579 1667 1434 1194 435 405 268
201 68 24
Bpu10I CC'TnA_GC
Cuts at: 3725 4314 7638 7663 8244 3725
Size: 589 3324 25 581 7512
Fragments arranged by size:
7512 3324 589 581 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2298 4143 8691 10156 11021 11319 2298
Size: 1845 4548 1465 865 298 3010
Fragments arranged by size:
4548 3010 1845 1465 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2720 3351 4102 4997 9042 10694 882
Size: 617 1221 631 751 895 4045 1652 2219
Fragments arranged by size:
4045 2219 1652 1221 895 751 631 617
BsaAI yAC'GTr
Cuts at: 493 1204 5464 7144 8329 9822 493
Size: 711 4260 1680 1185 1493 2702
Fragments arranged by size:
4260 2702 1680 1493 1185 711
BsaBI GATnn'nnATC
Cuts at: 12025 12025
Size: 12031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2703 4364 4853 5472
Size: 53 83 186 936 1170 1661 489 619
Cuts at: 5472 6046 6999 7709 8075 9838 10220 10855 275
Size: 574 953 710 366 1763 382 635 1451
Fragments arranged by size:
1763 1661 1451 1170 953 936 710 635
619 574 489 382 366 186 83 53
BsaWI w'CCGG_w
Cuts at: 3761 10464 10862 11136 11283 11694 3761
Size: 6703 398 274 147 411 4098
Fragments arranged by size:
6703 4098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3425 3455 3683 3713 3730
Size: 30 165 30 2022 30 228 30 17
Cuts at: 3730 3760 4003 4033 4561 4591 4693 4723 5042
Size: 30 243 30 528 30 102 30 319
Cuts at: 5042 5072 6321 6351 7536 7566 1178
Size: 30 1249 30 1185 30 5643
Fragments arranged by size:
5643 2022 1249 1185 528 319 243 228
165 102 30 30 30 30 30 30
30 30 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2163 2244 2558 3738 4629 4921 5087 5282
Size: 977 81 314 1180 891 292 166 195
Cuts at: 5282 5351 6701 7030 1186
Size: 69 1350 329 6187
Fragments arranged by size:
6187 1350 1180 977 891 329 314 292
195 166 81 69
BseYI C'CCAG_C
Cuts at: 2396 3203 3437 4510 4644 4866 4959 5355 6827
Size: 807 234 1073 134 222 93 396 1472
Cuts at: 6827 6920 7243 7590 7694 7725 7769 7851 7902
Size: 93 323 347 104 31 44 82 51
Cuts at: 7902 8045 8085 8150 8165 8195 8234 8355 8373
Size: 143 40 65 15 30 39 121 18
Cuts at: 8373 8391 8414 8669 8839 8952 9072 9111 9234
Size: 18 23 255 170 113 120 39 123
Cuts at: 9234 9248 11234 11730 2396
Size: 14 1986 496 2697
Fragments arranged by size:
2697 1986 1472 1073 807 496 396 347
323 255 234 222 170 143 134 123
121 120 113 104 93 93 82 65
51 44 40 39 39 31 30 23
18 18 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 3253 4251 4547 5876 6899 7073
Size: 573 573 944 998 296 1329 1023 174
Cuts at: 7073 7147 7430 8192 1163
Size: 74 283 762 5002
Fragments arranged by size:
5002 1329 1023 998 944 762 573 573
296 283 174 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8145 9481 10242 11270 1540
Size: 275 186 6144 1336 761 1028 2301
Fragments arranged by size:
6144 2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4339 4748 4863 6710 7347 10092 10177 11248 4339
Size: 409 115 1847 637 2745 85 1071 5122
Fragments arranged by size:
5122 2745 1847 1071 637 409 115 85
BsmI GAATG_Cn'
Cuts at: 2542 2679 3406 4314 6243 6760 8437 9542 9635
Size: 137 727 908 1929 517 1677 1105 93
Cuts at: 9635 2542
Size: 4938
Fragments arranged by size:
4938 1929 1677 1105 908 727 517 137
93
BsmBI CGTCTCn'nnnn_
Cuts at: 3103 7142 9136 3103
Size: 4039 1994 5998
Fragments arranged by size:
5998 4039 1994
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2433 4075 4277 4339 4725 4748
Size: 399 579 64 1642 202 62 386 23
Cuts at: 4748 4863 5028 5055 5454 6414 6710 7295 7347
Size: 115 165 27 399 960 296 585 52
Cuts at: 7347 7527 8013 8037 8157 8688 8951 9350 10092
Size: 180 486 24 120 531 263 399 742
Cuts at: 10092 10177 11248 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1642 1071 960 742 585 579 531
486 399 399 399 386 296 263 202
180 165 120 115 85 64 62 52
27 24 23
BspEI T'CCGG_A
Cuts at: 11694 11694
Size: 12031
BspHI T'CATG_A
Cuts at: 2628 8523 9920 11650 2628
Size: 5895 1397 1730 3009
Fragments arranged by size:
5895 3009 1730 1397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2728 3810 6921 7130 8220 8248 9386 844
Size: 1884 1082 3111 209 1090 28 1138 3489
Fragments arranged by size:
3489 3111 1884 1138 1090 1082 209 28
BsrBI CCG'CTC
Cuts at: 1394 1823 4370 6544 7053 8031 9918 1394
Size: 429 2547 2174 509 978 1887 3507
Fragments arranged by size:
3507 2547 2174 1887 978 509 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6457 9231 10526 10700 65
Size: 1183 5209 2774 1295 174 1396
Fragments arranged by size:
5209 2774 1396 1295 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3761 7308 7602 7831 8660 8728 10675
Size: 110 1951 3547 294 229 829 68 1947
Cuts at: 10675 10862 1700
Size: 187 2869
Fragments arranged by size:
3547 2869 1951 1947 829 294 229 187
110 68
BsrGI T'GTAC_A
Cuts at: 96 6291 96
Size: 6195 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12031
BssSI C'ACGA_G
Cuts at: 4227 6280 10091 11103 4227
Size: 2053 3811 1012 5155
Fragments arranged by size:
5155 3811 2053 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 7131 7170 7197 7579 7648 8098 8212 8596
Size: 6337 39 27 382 69 450 114 384
Cuts at: 8596 8752 8875 8962 794
Size: 156 123 87 3863
Fragments arranged by size:
6337 3863 450 384 382 156 123 114
87 69 39 27
BstBI TT'CG_AA
Cuts at: 10835 10835
Size: 12031
BstEII G'GTnAC_C
Cuts at: 1184 4780 1184
Size: 3596 8435
BstXI CCAn_nnnn'nTGG
Cuts at: 4189 4874 6004 8329 4189
Size: 685 1130 2325 7891
Fragments arranged by size:
7891 2325 1130 685
BstYI r'GATC_y
Cuts at: 1972 2636 3043 5350 5733 6360 7566 9361 9728
Size: 664 407 2307 383 627 1206 1795 367
Cuts at: 9728 10113 10130 11582 11668 11680 12026 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2307 1977 1795 1452 1206 664 627 407
385 383 367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3510 4518 3510
Size: 1008 11023
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 4218 4587 5400 6876 7797
Size: 552 939 351 1863 369 813 1476 921
Cuts at: 7797 8145 8453 8784 9059 9098 9174 9294 513
Size: 348 308 331 275 39 76 120 3250
Fragments arranged by size:
3250 1863 1476 939 921 813 552 369
351 348 331 308 275 120 76 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4433 4821 5688 7456 8498 487
Size: 683 56 3207 388 867 1768 1042 4020
Fragments arranged by size:
4020 3207 1768 1042 867 683 388 56
BtsI GCAGTG_nn'
Cuts at: 3036 4559 6708 7159 7498 8069 9547 10341 10361
Size: 1523 2149 451 339 571 1478 794 20
Cuts at: 10361 3036
Size: 4706
Fragments arranged by size:
4706 2149 1523 1478 794 571 451 339
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5127 5162 5616 5651 5842 5877 529
Size: 35 4563 35 454 35 191 35 6683
Fragments arranged by size:
6683 4563 454 191 35 35 35 35
DraI TTT'AAA
Cuts at: 2166 5260 9335 2166
Size: 3094 4075 4862
Fragments arranged by size:
4862 4075 3094
DraIII CAC_nnn'GTG
Cuts at: 7144 7170 7144
Size: 26 12005
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3282 7112 8227 11038 809
Size: 507 1966 3830 1115 2811 1802
Fragments arranged by size:
3830 2811 1966 1802 1115 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2305 2340 2584
Size: 54 1425 50 275 18 475 35 244
Cuts at: 2584 2796 3520 4290 4938 5863 6090 6326 7611
Size: 212 724 770 648 925 227 236 1285
Cuts at: 7611 8142 8331 8689 8730 8818 8998 9177 9478
Size: 531 189 358 41 88 180 179 301
Cuts at: 9478 10367 11996 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1285 925 889 770 724 648
531 475 358 301 275 244 236 227
212 189 180 179 88 54 50 43
41 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 8142 9478 1537
Size: 275 6330 1336 4090
Fragments arranged by size:
6330 4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3660 4494 5187 6648 6678 6696 7205 9961
Size: 2307 834 693 1461 30 18 509 2756
Cuts at: 9961 1353
Size: 3423
Fragments arranged by size:
3423 2756 2307 1461 834 693 509 30
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7544 7621 10618 10992 11138 387
Size: 1064 138 5955 77 2997 374 146 1280
Fragments arranged by size:
5955 2997 1280 1064 374 146 138 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2373 2673 2874 2898 2940 3333 4527
Size: 393 741 300 201 24 42 393 1194
Cuts at: 4527 4595 5184 5898 6081 6606 8246 8351 8756
Size: 68 589 714 183 525 1640 105 405
Cuts at: 8756 9024 10094 10691 11478 11746 1239
Size: 268 1070 597 787 268 1524
Fragments arranged by size:
1640 1524 1194 1070 787 741 714 597
589 525 405 393 393 300 268 268
201 183 105 68 42 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2735 7583 2735
Size: 4848 7183
EcoO109I rG'GnC_Cy
Cuts at: 2296 3032 3799 4223 4514 4970 5281 5317 7677
Size: 736 767 424 291 456 311 36 2360
Cuts at: 7677 7971 8154 8711 8922 2296
Size: 294 183 557 211 5405
Fragments arranged by size:
5405 2360 767 736 557 456 424 311
294 291 211 183 36
EcoRI G'AATT_C
Cuts at: 3360 4168 5367 9340 9718 3360
Size: 808 1199 3973 378 5673
Fragments arranged by size:
5673 3973 1199 808 378
EcoRV GAT'ATC
Cuts at: 2944 8124 2944
Size: 5180 6851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6913 6913
Size: 12031
FspI TGC'GCA
Cuts at: 10537 10537
Size: 12031
HaeII r_GCGC'y
Cuts at: 1678 2277 2706 4367 6049 7327 8078 8202 11178
Size: 599 429 1661 1682 1278 751 124 2976
Cuts at: 11178 1678
Size: 2531
Fragments arranged by size:
2976 2531 1682 1661 1278 751 599 429
124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2511 2543 3181
Size: 32 135 32 509 32 398 32 638
Cuts at: 3181 3213 3234 3266 3425 3457 3547 3579 4624
Size: 32 21 32 159 32 90 32 1045
Cuts at: 4624 4656 5042 5074 5114 5146 5309 5341 7268
Size: 32 386 32 40 32 163 32 1927
Cuts at: 7268 7300 8545 8577 8785 8817 9153 9185 10672
Size: 32 1245 32 208 32 336 32 1487
Cuts at: 10672 10704 10746 10778 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1927 1487 1245 1045 638 509 398
386 336 208 163 159 135 90 42
40 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2917 5226 6955 9375 9621 669
Size: 988 281 979 2309 1729 2420 246 3079
Fragments arranged by size:
3079 2420 2309 1729 988 979 281 246
HindIII A'AGCT_T
Cuts at: 748 5543 748
Size: 4795 7236
HpaI GTT'AAC
Cuts at: 9621 9621
Size: 12031
KasI G'GCGC_C
Cuts at: 2702 4363 6045 8074 2702
Size: 1661 1682 2029 6659
Fragments arranged by size:
6659 2029 1682 1661
KpnI G_GTAC'C
Cuts at: 9356 9356
Size: 12031
MfeI C'AATT_G
Cuts at: 3021 3372 9630 3021
Size: 351 6258 5422
Fragments arranged by size:
6258 5422 351
MluI A'CGCG_T
Cuts at: 7347 11907 7347
Size: 4560 7471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 3683 3726 11145 11329 1305
Size: 891 1487 43 7419 184 2007
Fragments arranged by size:
7419 2007 1487 891 184 43
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2307 2342 2586 2798 3522
Size: 54 1425 343 475 35 244 212 724
Cuts at: 3522 4292 4940 6092 7613 8333 8820 9000 9179
Size: 770 648 1152 1521 720 487 180 179
Cuts at: 9179 10
Size: 2862
Fragments arranged by size:
2862 1521 1425 1152 770 724 720 648
487 475 343 244 212 180 179 54
35
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 2966 3786 5712 7079 7799 7964
Size: 648 1713 87 820 1926 1367 720 165
Cuts at: 7964 9989 10348 10507 518
Size: 2025 359 159 2042
Fragments arranged by size:
2042 2025 1926 1713 1367 820 720 648
359 165 159 87
MspA1I CmG'CkG
Cuts at: 1606 2238 2441 3076 3502 4413 5656 6975 7760
Size: 632 203 635 426 911 1243 1319 785
Cuts at: 7760 7868 8455 8593 8884 9238 9447 10124 11272
Size: 108 587 138 291 354 209 677 1148
Cuts at: 11272 11517 1606
Size: 245 2120
Fragments arranged by size:
2120 1319 1243 1148 911 785 677 635
632 587 426 354 291 245 209 203
138 108
NaeI GCC'GGC
Cuts at: 7310 7604 7833 8662 7310
Size: 294 229 829 10679
Fragments arranged by size:
10679 829 294 229
NarI GG'CG_CC
Cuts at: 2703 4364 6046 8075 2703
Size: 1661 1682 2029 6659
Fragments arranged by size:
6659 2029 1682 1661
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 4218 5400 6876 8784 9174
Size: 552 939 351 1863 1182 1476 1908 390
Cuts at: 9174 513
Size: 3370
Fragments arranged by size:
3370 1908 1863 1476 1182 939 552 390
351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12031
NgoMIV G'CCGG_C
Cuts at: 7308 7602 7831 8660 7308
Size: 294 229 829 10679
Fragments arranged by size:
10679 829 294 229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12031
NotI GC'GGCC_GC
Cuts at: 9478 9478
Size: 12031
NruI TCG'CGA
Cuts at: 5673 5673
Size: 12031
NsiI A_TGCA'T
Cuts at: 5877 5877
Size: 12031
NspI r_CATG'y
Cuts at: 1132 1229 3791 3925 4150 4510 4603 6835 7078
Size: 97 2562 134 225 360 93 2232 243
Cuts at: 7078 7753 7948 8053 8350 8386 8404 8755 8965
Size: 675 195 105 297 36 18 351 210
Cuts at: 8965 9389 10934 1132
Size: 424 1545 2229
Fragments arranged by size:
2562 2232 2229 1545 675 424 360 351
297 243 225 210 195 134 105 97
93 36 18
PacI TTA_AT'TAA
Cuts at: 2598 2598
Size: 12031
PasI CC'CwG_GG
Cuts at: 1348 3574 3667 5755 7096 8503 1348
Size: 2226 93 2088 1341 1407 4876
Fragments arranged by size:
4876 2226 2088 1407 1341 93
PciI A'CATG_T
Cuts at: 3787 3921 10930 3787
Size: 134 7009 4888
Fragments arranged by size:
7009 4888 134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3356 7228 7339 1108
Size: 2248 3872 111 5800
Fragments arranged by size:
5800 3872 2248 111
PmeI GTTT'AAAC
Cuts at: 9335 9335
Size: 12031
PmlI CAC'GTG
Cuts at: 7144 7144
Size: 12031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 6172 6204 10100 10132 11613 11645 3683
Size: 32 2457 32 3896 32 1481 32 4069
Fragments arranged by size:
4069 3896 2457 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3799 4223 4970 5317 7677 7971 8711 8922 3799
Size: 424 747 347 2360 294 740 211 6908
Fragments arranged by size:
6908 2360 747 740 424 347 294 211
PshAI GACnn'nnGTC
Cuts at: 1613 4126 1613
Size: 2513 9518
PsiI TTA'TAA
Cuts at: 9601 9601
Size: 12031
PspOMI G'GGCC_C
Cuts at: 2429 8033 8153 2429
Size: 5604 120 6307
Fragments arranged by size:
6307 5604 120
PspXI vC'TCGA_Gb
Cuts at: 2707 2707
Size: 12031
PstI C_TGCA'G
Cuts at: 830 3043 3507 5994 6504 6715 7584 7867 8424
Size: 2213 464 2487 510 211 869 283 557
Cuts at: 8424 8601 8880 8895 9383 830
Size: 177 279 15 488 3478
Fragments arranged by size:
3478 2487 2213 869 557 510 488 464
283 279 211 177 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12031
PvuII CAG'CTG
Cuts at: 3076 3502 5656 6975 7760 8593 9238 3076
Size: 426 2154 1319 785 833 645 5869
Fragments arranged by size:
5869 2154 1319 833 785 645 426
RsrII CG'GwC_CG
Cuts at: 2027 2027
Size: 12031
SacII CC_GC'GG
Cuts at: 8456 8456
Size: 12031
SalI G'TCGA_C
Cuts at: 1936 6953 9373 1936
Size: 5017 2420 4594
Fragments arranged by size:
5017 4594 2420
SanDI GG'GwC_CC
Cuts at: 7677 7971 8922 7677
Size: 294 951 10786
Fragments arranged by size:
10786 951 294
SapI GCTCTTCn'nnn_
Cuts at: 3660 4494 7205 3660
Size: 834 2711 8486
Fragments arranged by size:
8486 2711 834
SbfI CC_TGCA'GG
Cuts at: 9383 9383
Size: 12031
ScaI AGT'ACT
Cuts at: 10279 11991 10279
Size: 1712 10319
SexAI A'CCwGG_T
Cuts at: 2521 3888 8022 9027 2521
Size: 1367 4134 1005 5525
Fragments arranged by size:
5525 4134 1367 1005
SfcI C'TryA_G
Cuts at: 826 1045 2937 3039 3503 4752 5346 5686 5990
Size: 219 1892 102 464 1249 594 340 304
Cuts at: 5990 6500 6711 7580 7863 8420 8597 8876 8891
Size: 510 211 869 283 557 177 279 15
Cuts at: 8891 9379 10514 11195 11386 826
Size: 488 1135 681 191 1471
Fragments arranged by size:
1892 1471 1249 1135 869 681 594 557
510 488 464 340 304 283 279 219
211 191 177 102 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2304 8141 2304
Size: 5837 6194
SfoI GGC'GCC
Cuts at: 2704 4365 6047 8076 2704
Size: 1661 1682 2029 6659
Fragments arranged by size:
6659 2029 1682 1661
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12031
SgrAI Cr'CCGG_yG
Cuts at: 1700 3761 7602 1700
Size: 2061 3841 6129
Fragments arranged by size:
6129 3841 2061
SmaI CCC'GGG
Cuts at: 1872 4068 6124 7675 7977 8059 9358 1872
Size: 2196 2056 1551 302 82 1299 4545
Fragments arranged by size:
4545 2196 2056 1551 1299 302 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2313 2707 4158 8706 10135 11036
Size: 197 926 370 394 1451 4548 1429 901
Cuts at: 11036 11298 820
Size: 262 1553
Fragments arranged by size:
4548 1553 1451 1429 926 901 394 370
262 197
SnaBI TAC'GTA
Cuts at: 493 9822 493
Size: 9329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12031
SphI G_CATG'C
Cuts at: 1132 4150 4603 6835 7078 7948 8053 8350 8386
Size: 3018 453 2232 243 870 105 297 36
Cuts at: 8386 8404 8755 9389 1132
Size: 18 351 634 3774
Fragments arranged by size:
3774 3018 2232 870 634 453 351 297
243 105 36 18
SspI AAT'ATT
Cuts at: 5 52 3953 9955 5
Size: 47 3901 6002 2081
Fragments arranged by size:
6002 3901 2081 47
StuI AGG'CCT
Cuts at: 1517 2160 2565 8116 8493 8513 8984 1517
Size: 643 405 5551 377 20 471 4564
Fragments arranged by size:
5551 4564 643 471 405 377 20
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 4188 4218 5400 5590 6606
Size: 552 939 351 1833 30 1182 190 1016
Cuts at: 6606 6876 7419 8784 9174 9470 513
Size: 270 543 1365 390 296 3074
Fragments arranged by size:
3074 1833 1365 1182 1016 939 552 543
390 351 296 270 190 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2291 2574 4231 4322 4581 5747 6039
Size: 1255 711 283 1657 91 259 1166 292
Cuts at: 6039 6480 7109 8194 8209 10058 10075 10228 10413
Size: 441 629 1085 15 1849 17 153 185
Cuts at: 10413 10858 325
Size: 445 1498
Fragments arranged by size:
1849 1657 1498 1255 1166 1085 711 629
445 441 292 283 259 185 153 91
17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6291 10277 11989
Size: 274 80 33 51 1443 4314 3986 1712
Cuts at: 11989 96
Size: 138
Fragments arranged by size:
4314 3986 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3028 3060 4693 4725 6321 6353 11991 12023 3028
Size: 32 1633 32 1596 32 5638 32 3036
Fragments arranged by size:
5638 3036 1633 1596 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4552 5471 5576 1316
Size: 3236 919 105 7771
Fragments arranged by size:
7771 3236 919 105
XbaI T'CTAG_A
Cuts at: 9367 9367
Size: 12031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4351 6917 8791 4351
Size: 2566 1874 7591
Fragments arranged by size:
7591 2566 1874
XhoI C'TCGA_G
Cuts at: 1943 2707 1943
Size: 764 11267
XmaI C'CCGG_G
Cuts at: 1870 4066 6122 7673 7975 8057 9356 1870
Size: 2196 2056 1551 302 82 1299 4545
Fragments arranged by size:
4545 2196 2056 1551 1299 302 82
XmnI GAAnn'nnTTC
Cuts at: 4397 5723 10160 4397
Size: 1326 4437 6268
Fragments arranged by size:
6268 4437 1326
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5473 9839 276
Size: 53 83 186 936 3939 4366 2468
Fragments arranged by size:
4366 3939 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I EcoICRI FseI FspAI
PfoI PsrI SacI SrfI SwaI