Restriction map for
pFN21AB0056
(Circular) (Six-base) MAPSORT of: pFN21AB0056.seq Check: 9301 from: 1 to: 9304
pFN21AB0056 9304 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3547 3577 4333 5275 6220 6250 3547
Size: 30 756 942 945 30 6601
Fragments arranged by size:
6601 945 942 756 30 30
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7114 278
Size: 53 83 186 936 5578 2468
Fragments arranged by size:
5578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6084 6450 6647 1937
Size: 4147 366 197 4594
Fragments arranged by size:
4594 4147 366 197
Acc65I G'GTAC_C
Cuts at: 6625 6625
Size: 9304
AclI AA'CG_TT
Cuts at: 1563 7431 7804 1563
Size: 5868 373 3063
Fragments arranged by size:
5868 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2700 3138 3351 3663 3992 5948 6030 7367
Size: 1068 438 213 312 329 1956 82 1337
Cuts at: 7367 8751 9019 1632
Size: 1384 268 1917
Fragments arranged by size:
1956 1917 1384 1337 1068 438 329 312
268 213 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9107
AflIII A'CryG_T
Cuts at: 1203 8203 9180 1203
Size: 7000 977 1327
Fragments arranged by size:
7000 1327 977
AgeI A'CCGG_T
Cuts at: 4354 8135 4354
Size: 3781 5523
AhdI GACnn_n'nnGTC
Cuts at: 8033 8033
Size: 9304
AleI CACnn'nnGTG
Cuts at: 1166 3710 1166
Size: 2544 6760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6098 6130 6098
Size: 32 9272
AlwNI CAG_nnn'CTG
Cuts at: 2320 2335 3098 3329 3527 3970 4570 5314 5372
Size: 15 763 231 198 443 600 744 58
Cuts at: 5372 5501 5626 5836 5980 7047 8619 2320
Size: 129 125 210 144 1067 1572 3005
Fragments arranged by size:
3005 1572 1067 763 744 600 443 231
210 198 144 129 125 58 15
ApaI G_GGCC'C
Cuts at: 2589 2666 2841 2867 2992 3260 3278 3406 4661
Size: 77 175 26 125 268 18 128 1255
Cuts at: 4661 4907 6173 6540 2589
Size: 246 1266 367 5353
Fragments arranged by size:
5353 1266 1255 367 268 246 175 128
125 77 26 18
ApaLI G'TGCA_C
Cuts at: 7361 8517 7361
Size: 1156 8148
ApoI r'AATT_y
Cuts at: 1493 2510 2611 6613 6839 6991 9100 1493
Size: 1017 101 4002 226 152 2109 1697
Fragments arranged by size:
4002 2109 1697 1017 226 152 101
AseI AT'TA_AT
Cuts at: 160 1032 7858 160
Size: 872 6826 1606
Fragments arranged by size:
6826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9304
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2144 2390 2502 2697 2868 4269
Size: 73 74 127 246 112 195 171 1401
Cuts at: 4269 4773 5301 6225 6528 6567 6629 1870
Size: 504 528 924 303 39 62 4545
Fragments arranged by size:
4545 1401 924 528 504 303 246 195
171 127 112 74 73 62 39
AvrII C'CTAG_G
Cuts at: 4667 5252 4667
Size: 585 8719
BamHI G'GATC_C
Cuts at: 4360 6634 7001 4360
Size: 2274 367 6663
Fragments arranged by size:
6663 2274 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3499 6625 8080 618
Size: 325 217 627 1712 3126 1455 1842
Fragments arranged by size:
3126 1842 1712 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2589 2666 2841 2867 2992 3260 3278 3406
Size: 1198 77 175 26 125 268 18 128
Cuts at: 3406 4016 4295 4661 4692 4892 4907 5034 5607
Size: 610 279 366 31 200 15 127 573
Cuts at: 5607 6055 6173 6540 6623 1391
Size: 448 118 367 83 4072
Fragments arranged by size:
4072 1198 610 573 448 367 366 279
268 200 175 128 127 125 118 83
77 31 26 18 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4381 4510 6424 928
Size: 971 2482 129 1914 3808
Fragments arranged by size:
3808 2482 1914 971 129
BbvCI CC'TCA_GC
Cuts at: 3069 5797 3069
Size: 2728 6576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7495 7529 1903
Size: 34 5558 34 3678
Fragments arranged by size:
5558 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2778 5640 6486 7196 8406 2778
Size: 2862 846 710 1210 3676
Fragments arranged by size:
3676 2862 1210 846 710
BclI T'GATC_A
Cuts at: 1548 2950 1548
Size: 1402 7902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2871 5331 6033
Size: 107 122 71 631 605 1199 2460 702
Cuts at: 6033 7915 136
Size: 1882 1525
Fragments arranged by size:
2460 1882 1525 1199 702 631 605 122
107 71
BglII A'GATC_T
Cuts at: 5673 9299 5673
Size: 3626 5678
BlpI GC'TnA_GC
Cuts at: 2313 5559 6721 2313
Size: 3246 1162 4896
Fragments arranged by size:
4896 3246 1162
Bme1580I G_kGCm'C
Cuts at: 1790 2434 2589 2666 2841 2867 2992 3260 3278
Size: 644 155 77 175 26 125 268 18
Cuts at: 3278 3406 3502 4038 4349 4661 4907 5592 6173
Size: 128 96 536 311 312 246 685 581
Cuts at: 6173 6540 7365 8521 1790
Size: 367 825 1156 2573
Fragments arranged by size:
2573 1156 825 685 644 581 536 367
312 311 268 246 175 155 128 125
96 77 26 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2722 3280 3761
Size: 416 53 83 399 24 1305 558 481
Cuts at: 3761 3833 4025 5402 5535 5536 5931 7035 7993
Size: 72 192 1377 133 1 395 1104 958
Cuts at: 7993 442
Size: 1753
Fragments arranged by size:
1753 1377 1305 1104 958 558 481 416
399 395 192 133 83 72 53 24
1
BmtI G_CTAG'C
Cuts at: 1056 6264 1056
Size: 5208 4096
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4494 5626 5909 4494
Size: 1132 283 7889
Fragments arranged by size:
7889 1132 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2163 2678 3183 3582 3594 3669 4193 4467
Size: 924 515 505 399 12 75 524 274
Cuts at: 4467 5214 5904 7964 1239
Size: 747 690 2060 2579
Fragments arranged by size:
2579 2060 924 747 690 524 515 505
399 274 75 12
Bpu10I CC'TnA_GC
Cuts at: 2875 3069 5797 6498 2875
Size: 194 2728 701 5681
Fragments arranged by size:
5681 2728 701 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3458 4279 5144 5807 6386 7429 8294 8592
Size: 453 821 865 663 579 1043 865 298
Cuts at: 8592 3005
Size: 3717
Fragments arranged by size:
3717 1043 865 865 821 663 579 453
298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5251 7967 882
Size: 617 3752 2716 2219
Fragments arranged by size:
3752 2716 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2079 7095 493
Size: 711 875 5016 2702
Fragments arranged by size:
5016 2702 875 711
BsaBI GATnn'nnATC
Cuts at: 9298 9298
Size: 9304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2351 7111 7493 8128
Size: 53 83 186 936 818 4760 382 635
Cuts at: 8128 275
Size: 1451
Fragments arranged by size:
4760 1451 936 818 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4354 7737 8135 8409 8556 8967 4354
Size: 3383 398 274 147 411 4691
Fragments arranged by size:
4691 3383 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2583 2613 3544 3574 3667
Size: 30 165 30 1180 30 931 30 93
Cuts at: 3667 3697 6098 6128 6548 6572 6578 6602 1178
Size: 30 2401 30 420 24 6 24 3880
Fragments arranged by size:
3880 2401 1180 931 420 165 93 30
30 30 30 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3074 3440 3813 3927 4196 4470 4483 4748
Size: 1888 366 373 114 269 274 13 265
Cuts at: 4748 4761 4857 5191 5602 5901 6000 6569 1186
Size: 13 96 334 411 299 99 569 3921
Fragments arranged by size:
3921 1888 569 411 373 366 334 299
274 269 265 114 99 96 13 13
BseYI C'CCAG_C
Cuts at: 2380 2840 3322 3556 4502 4575 5006 5606 5654
Size: 460 482 234 946 73 431 600 48
Cuts at: 5654 5785 5866 6060 6188 6441 8507 9003 2380
Size: 131 81 194 128 253 2066 496 2681
Fragments arranged by size:
2681 2066 946 600 496 482 460 431
253 234 194 131 128 81 73 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3576 4590 5729 1163
Size: 573 1840 1014 1139 4738
Fragments arranged by size:
4738 1840 1139 1014 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2014 6754 7515 8543 1540
Size: 275 186 13 4740 761 1028 2301
Fragments arranged by size:
4740 2301 1028 761 275 186 13
BsiHKAI G_wGCw'C
Cuts at: 2458 4126 7365 7450 8521 2458
Size: 1668 3239 85 1071 3241
Fragments arranged by size:
3241 3239 1668 1071 85
BsmI GAATG_Cn'
Cuts at: 5218 6815 6908 5218
Size: 1597 93 7614
Fragments arranged by size:
7614 1597 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6404 6404
Size: 9304
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2434 2458 2589 2666 2841 2867 2992
Size: 399 644 24 131 77 175 26 125
Cuts at: 2992 3260 3278 3406 3502 4016 4038 4126 4295
Size: 268 18 128 96 514 22 88 169
Cuts at: 4295 4349 4661 4692 4892 4907 5034 5592 5607
Size: 54 312 31 200 15 127 558 15
Cuts at: 5607 6055 6173 6540 6623 7365 7450 8521 1391
Size: 448 118 367 83 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 558 514 448 399
367 312 268 200 175 169 131 128
127 125 118 96 88 85 83 77
54 31 26 24 22 18 15 15
BspEI T'CCGG_A
Cuts at: 8967 8967
Size: 9304
BspHI T'CATG_A
Cuts at: 2689 7193 8923 2689
Size: 4504 1730 3070
Fragments arranged by size:
4504 3070 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3547 3577 4333 5275 6220 6250 6659 844
Size: 2703 30 756 942 945 30 409 3489
Fragments arranged by size:
3489 2703 945 942 756 409 30 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2312 3400 3430 4819 6511 7191 1394
Size: 429 489 1088 30 1389 1692 680 3507
Fragments arranged by size:
3507 1692 1389 1088 680 489 429 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 2157 2224 7799 7973 65
Size: 1183 909 67 5575 174 1396
Fragments arranged by size:
5575 1396 1183 909 174 67
BsrFI r'CCGG_y
Cuts at: 1700 1810 2338 4131 4354 5001 5878 7948 8135
Size: 110 528 1793 223 647 877 2070 187
Cuts at: 8135 1700
Size: 2869
Fragments arranged by size:
2869 2070 1793 877 647 528 223 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9304
BssSI C'ACGA_G
Cuts at: 5520 7364 8376 5520
Size: 1844 1012 6448
Fragments arranged by size:
6448 1844 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 3461 3527 5314 794
Size: 1541 1126 66 1787 4784
Fragments arranged by size:
4784 1787 1541 1126 66
BstBI TT'CG_AA
Cuts at: 3756 8108 3756
Size: 4352 4952
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9304
BstXI CCAn_nnnn'nTGG
Cuts at: 3769 5014 5399 5578 3769
Size: 1245 385 179 7495
Fragments arranged by size:
7495 1245 385 179
BstYI r'GATC_y
Cuts at: 1972 2100 4360 5673 5977 6634 7001 7386 7403
Size: 128 2260 1313 304 657 367 385 17
Cuts at: 7403 8855 8941 8953 9299 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2260 1977 1452 1313 657 385 367 346
304 128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4653 4899 4914 5614 5634 4653
Size: 246 15 700 20 8323
Fragments arranged by size:
8323 700 246 20 15
BtgI C'CryG_G
Cuts at: 513 1065 2128 2449 2743 5013 5688 6078 513
Size: 552 1063 321 294 2270 675 390 3739
Fragments arranged by size:
3739 2270 1063 675 552 390 321 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3045 487
Size: 683 56 1819 6746
Fragments arranged by size:
6746 1819 683 56
BtsI GCAGTG_nn'
Cuts at: 2333 3399 3751 5656 5741 5801 6820 7614 7634
Size: 1066 352 1905 85 60 1019 794 20
Cuts at: 7634 2333
Size: 4003
Fragments arranged by size:
4003 1905 1066 1019 794 352 85 60
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9269
DraI TTT'AAA
Cuts at: 6608 6608
Size: 9304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2228 8311 809
Size: 507 912 6083 1802
Fragments arranged by size:
6083 1802 912 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5384 6025 6751
Size: 54 1425 50 275 18 3554 641 726
Cuts at: 6751 7640 9269 8
Size: 889 1629 43
Fragments arranged by size:
3554 1629 1425 889 726 641 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6751 1537
Size: 275 4939 4090
Fragments arranged by size:
4939 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2991 3417 3438 3639 3807 7234 1353
Size: 1638 426 21 201 168 3427 3423
Fragments arranged by size:
3427 3423 1638 426 201 168 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2591 2781 4511 5714 7891 8265
Size: 1064 138 1002 190 1730 1203 2177 374
Cuts at: 8265 8411 387
Size: 146 1280
Fragments arranged by size:
2177 1730 1280 1203 1064 1002 374 190
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2678 2700 3138 3183 3351 3582
Size: 393 531 515 22 438 45 168 231
Cuts at: 3582 3594 3663 3669 3992 4193 4467 5214 5904
Size: 12 69 6 323 201 274 747 690
Cuts at: 5904 5948 6030 7367 7964 8751 9019 1239
Size: 44 82 1337 597 787 268 1524
Fragments arranged by size:
1524 1337 787 747 690 597 531 515
438 393 323 274 268 231 201 168
82 69 45 44 22 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3684 4666 5151 6531 3684
Size: 982 485 1380 6457
Fragments arranged by size:
6457 1380 982 485
EcoO109I rG'GnC_Cy
Cuts at: 2662 2810 2864 2988 3215 3256 3257 3274 3275
Size: 148 54 124 227 41 1 17 1
Cuts at: 3275 3305 3658 3797 4108 4657 4658 4671 4903
Size: 30 353 139 311 549 1 13 232
Cuts at: 4903 4910 4945 5123 5177 6170 6249 6341 6375
Size: 7 35 178 54 993 79 92 34
Cuts at: 6375 6536 6537 6564 2662
Size: 161 1 27 5402
Fragments arranged by size:
5402 993 549 353 311 232 227 178
161 148 139 124 92 79 54 54
41 35 34 30 27 17 13 7
1 1 1 1
EcoRI G'AATT_C
Cuts at: 6613 6991 6613
Size: 378 8926
FseI GG_CCGG'CC
Cuts at: 4135 4135
Size: 9304
FspI TGC'GCA
Cuts at: 7810 7810
Size: 9304
HaeII r_GCGC'y
Cuts at: 1678 3451 5626 8451 1678
Size: 1773 2175 2825 2531
Fragments arranged by size:
2825 2531 2175 1773
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2583 2615 3074 3106 4168
Size: 32 135 32 1011 32 459 32 1062
Cuts at: 4168 4200 5420 5452 5552 5584 5969 6001 6548
Size: 32 1220 32 100 32 385 32 547
Cuts at: 6548 6580 7945 7977 8019 8051 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 1220 1062 1011 547 459 385
135 100 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3004 4702 6451 6648 6894 669
Size: 988 281 1066 1698 1749 197 246 3079
Fragments arranged by size:
3079 1749 1698 1066 988 281 246 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9304
HpaI GTT'AAC
Cuts at: 6894 6894
Size: 9304
KpnI G_GTAC'C
Cuts at: 6629 6629
Size: 9304
MfeI C'AATT_G
Cuts at: 6903 6903
Size: 9304
MluI A'CGCG_T
Cuts at: 9180 9180
Size: 9304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 2385 2540 2937 4585 8418 8602 1305
Size: 1002 78 155 397 1648 3833 184 2007
Fragments arranged by size:
3833 2007 1648 1002 397 184 155 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6027 10
Size: 54 1425 343 4195 3287
Fragments arranged by size:
4195 3287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 2688 3710 5066 7262 7621 7780
Size: 648 1297 225 1022 1356 2196 359 159
Cuts at: 7780 518
Size: 2042
Fragments arranged by size:
2196 2042 1356 1297 1022 648 359 225
159
MspA1I CmG'CkG
Cuts at: 1606 2114 2320 2745 3949 4406 4707 6094 6720
Size: 508 206 425 1204 457 301 1387 626
Cuts at: 6720 7397 8545 8790 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1387 1204 1148 677 626 508 457
425 301 245 206
NaeI GCC'GGC
Cuts at: 2340 4133 5880 2340
Size: 1793 1747 5764
Fragments arranged by size:
5764 1793 1747
NcoI C'CATG_G
Cuts at: 513 1065 2128 2449 5013 5688 6078 513
Size: 552 1063 321 2564 675 390 3739
Fragments arranged by size:
3739 2564 1063 675 552 390 321
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9304
NgoMIV G'CCGG_C
Cuts at: 2338 4131 5878 2338
Size: 1793 1747 5764
Fragments arranged by size:
5764 1793 1747
NheI G'CTAG_C
Cuts at: 1052 6260 1052
Size: 5208 4096
NotI GC'GGCC_GC
Cuts at: 6751 6751
Size: 9304
NspI r_CATG'y
Cuts at: 1132 1229 3439 6662 8207 1132
Size: 97 2210 3223 1545 2229
Fragments arranged by size:
3223 2229 2210 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2806 3281 4063 5729 1348
Size: 1458 475 782 1666 4923
Fragments arranged by size:
4923 1666 1458 782 475
PciI A'CATG_T
Cuts at: 8203 8203
Size: 9304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3299 5714 5836 1108
Size: 2191 2415 122 4576
Fragments arranged by size:
4576 2415 2191 122
PfoI T'CCnGG_A
Cuts at: 5598 6306 5598
Size: 708 8596
PmeI GTTT'AAAC
Cuts at: 6608 6608
Size: 9304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6098 6130 7373 7405 8886 8918 6098
Size: 32 1243 32 1481 32 6484
Fragments arranged by size:
6484 1481 1243 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3215 3305 3797 5123 5177 6341 6375 6564 3215
Size: 90 492 1326 54 1164 34 189 5955
Fragments arranged by size:
5955 1326 1164 492 189 90 54 34
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9304
PsiI TTA'TAA
Cuts at: 6874 6874
Size: 9304
PspOMI G'GGCC_C
Cuts at: 2585 2662 2837 2863 2988 3256 3274 3402 4657
Size: 77 175 26 125 268 18 128 1255
Cuts at: 4657 4903 6169 6536 2585
Size: 246 1266 367 5353
Fragments arranged by size:
5353 1266 1255 367 268 246 175 128
125 77 26 18
PstI C_TGCA'G
Cuts at: 830 2139 2526 3574 5132 6018 6183 6656 830
Size: 1309 387 1048 1558 886 165 473 3478
Fragments arranged by size:
3478 1558 1309 1048 886 473 387 165
PvuI CG_AT'CG
Cuts at: 2001 2014 2001
Size: 13 9291
PvuII CAG'CTG
Cuts at: 6094 6094
Size: 9304
SacII CC_GC'GG
Cuts at: 2746 2746
Size: 9304
SalI G'TCGA_C
Cuts at: 1936 6449 6646 1936
Size: 4513 197 4594
Fragments arranged by size:
4594 4513 197
SanDI GG'GwC_CC
Cuts at: 5177 6564 5177
Size: 1387 7917
SapI GCTCTTCn'nnn_
Cuts at: 3438 3639 3438
Size: 201 9103
SbfI CC_TGCA'GG
Cuts at: 2526 6656 2526
Size: 4130 5174
ScaI AGT'ACT
Cuts at: 7552 9264 7552
Size: 1712 7592
SexAI A'CCwGG_T
Cuts at: 5398 5398
Size: 9304
SfcI C'TryA_G
Cuts at: 826 1045 2135 2522 2708 3570 4844 5128 5859
Size: 219 1090 387 186 862 1274 284 731
Cuts at: 5859 6014 6179 6421 6652 7787 8468 8659 826
Size: 155 165 242 231 1135 681 191 1471
Fragments arranged by size:
1471 1274 1135 1090 862 731 681 387
284 242 231 219 191 186 165 155
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9304
SgrAI Cr'CCGG_yG
Cuts at: 1700 4354 1700
Size: 2654 6650
SmaI CCC'GGG
Cuts at: 1872 2699 5303 6631 1872
Size: 827 2604 1328 4545
Fragments arranged by size:
4545 2604 1328 827
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2502 3020 3473 4294 5159 5822
Size: 197 926 559 518 453 821 865 663
Cuts at: 5822 6401 6528 7408 8309 8571 820
Size: 579 127 880 901 262 1553
Fragments arranged by size:
1553 926 901 880 865 821 663 579
559 518 453 262 197 127
SnaBI TAC'GTA
Cuts at: 493 2079 7095 493
Size: 1586 5016 2702
Fragments arranged by size:
5016 2702 1586
SpeI A'CTAG_T
Cuts at: 152 2236 5423 152
Size: 2084 3187 4033
Fragments arranged by size:
4033 3187 2084
SphI G_CATG'C
Cuts at: 1132 3439 6662 1132
Size: 2307 3223 3774
Fragments arranged by size:
3774 3223 2307
SspI AAT'ATT
Cuts at: 5 52 7228 5
Size: 47 7176 2081
Fragments arranged by size:
7176 2081 47
StuI AGG'CCT
Cuts at: 1517 5191 6467 1517
Size: 3674 1276 4354
Fragments arranged by size:
4354 3674 1276
StyI C'CwwG_G
Cuts at: 513 1065 2031 2128 2449 2553 3858 4104 4622
Size: 552 966 97 321 104 1305 246 518
Cuts at: 4622 4667 5013 5021 5252 5688 6078 6334 6503
Size: 45 346 8 231 436 390 256 169
Cuts at: 6503 6743 513
Size: 240 3074
Fragments arranged by size:
3074 1305 966 552 518 436 390 346
321 256 246 240 231 169 104 97
45 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 4156 4751 5373 6455 7331 7348
Size: 1255 882 1694 595 622 1082 876 17
Cuts at: 7348 7501 7686 8131 325
Size: 153 185 445 1498
Fragments arranged by size:
1694 1498 1255 1082 882 876 622 595
445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7550 9262 96
Size: 274 80 33 51 1443 5573 1712 138
Fragments arranged by size:
5573 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2715 2747 3068 3100 3558 3590 5895 5927 9264
Size: 32 321 32 458 32 2305 32 3337
Cuts at: 9264 9296 2715
Size: 32 2723
Fragments arranged by size:
3337 2723 2305 458 321 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5400 1316
Size: 4084 5220
XbaI T'CTAG_A
Cuts at: 4225 6640 4225
Size: 2415 6889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2456 3095 4371 2456
Size: 639 1276 7389
Fragments arranged by size:
7389 1276 639
XhoI C'TCGA_G
Cuts at: 1943 2502 6528 1943
Size: 559 4026 4719
Fragments arranged by size:
4719 4026 559
XmaI C'CCGG_G
Cuts at: 1870 2697 5301 6629 1870
Size: 827 2604 1328 4545
Fragments arranged by size:
4545 2604 1328 827
XmnI GAAnn'nnTTC
Cuts at: 3816 7433 3816
Size: 3617 5687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7112 276
Size: 53 83 186 936 5578 2468
Fragments arranged by size:
5578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SfiI SfoI
SrfI SwaI