Restriction map for
pFN21AB0048
(Circular) (Six-base) MAPSORT of: pFN21AB0048.seq Check: 6992 from: 1 to: 9973
pFN21AB0048 9973 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7783 278
Size: 53 83 186 936 6247 2468
Fragments arranged by size:
6247 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3731 4480 5476 7316 1937
Size: 1794 749 996 1840 4594
Fragments arranged by size:
4594 1840 1794 996 749
Acc65I G'GTAC_C
Cuts at: 7294 7294
Size: 9973
AclI AA'CG_TT
Cuts at: 1563 8100 8473 1563
Size: 6537 373 3063
Fragments arranged by size:
6537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 5490 5853 5990 6000 6033 6204 6807
Size: 552 3306 363 137 10 33 171 603
Cuts at: 6807 7112 8036 9420 9688 1632
Size: 305 924 1384 268 1917
Fragments arranged by size:
3306 1917 1384 924 603 552 363 305
268 171 137 33 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9776
AflIII A'CryG_T
Cuts at: 1203 8872 9849 1203
Size: 7669 977 1327
Fragments arranged by size:
7669 1327 977
AgeI A'CCGG_T
Cuts at: 8804 8804
Size: 9973
AhdI GACnn_n'nnGTC
Cuts at: 2488 2923 7144 7174 8702 2488
Size: 435 4221 30 1528 3759
Fragments arranged by size:
4221 3759 1528 435 30
AleI CACnn'nnGTG
Cuts at: 1166 2534 1166
Size: 1368 8605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9973
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5079 5111 5079
Size: 32 9941
AlwNI CAG_nnn'CTG
Cuts at: 2342 2851 5456 7716 9288 2342
Size: 509 2605 2260 1572 3027
Fragments arranged by size:
3027 2605 2260 1572 509
ApaI G_GGCC'C
Cuts at: 5517 5517
Size: 9973
ApaLI G'TGCA_C
Cuts at: 8030 9186 8030
Size: 1156 8817
ApoI r'AATT_y
Cuts at: 1493 2598 2841 3003 3245 3366 4294 4816 6148
Size: 1105 243 162 242 121 928 522 1332
Cuts at: 6148 6367 6608 7282 7508 7660 9769 1493
Size: 219 241 674 226 152 2109 1697
Fragments arranged by size:
2109 1697 1332 1105 928 674 522 243
242 241 226 219 162 152 121
AseI AT'TA_AT
Cuts at: 160 1032 8527 160
Size: 872 7495 1606
Fragments arranged by size:
7495 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9973
AvaI C'yCGr_G
Cuts at: 1870 1943 4994 5129 6876 7298 1870
Size: 73 3051 135 1747 422 4545
Fragments arranged by size:
4545 3051 1747 422 135 73
BamHI G'GATC_C
Cuts at: 4984 5943 7303 7670 4984
Size: 959 1360 367 7287
Fragments arranged by size:
7287 1360 959 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5413 7294 8749 618
Size: 325 217 627 3626 1881 1455 1842
Fragments arranged by size:
3626 1881 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2056 3635 4845 5517 7292 1391
Size: 665 1579 1210 672 1775 4072
Fragments arranged by size:
4072 1775 1579 1210 672 665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2558 3779 4506 6651 7202 928
Size: 971 659 1221 727 2145 551 3699
Fragments arranged by size:
3699 2145 1221 971 727 659 551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4403 4437 5110 5144 8164 8198 1903
Size: 34 2466 34 673 34 3020 34 3678
Fragments arranged by size:
3678 3020 2466 673 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3601 4143 7865 9075 3601
Size: 542 3722 1210 4499
Fragments arranged by size:
4499 3722 1210 542
BclI T'GATC_A
Cuts at: 1548 2938 3049 3361 4531 1548
Size: 1390 111 312 1170 6990
Fragments arranged by size:
6990 1390 1170 312 111
BfrBI ATG'CAT
Cuts at: 4633 4633
Size: 9973
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8584 136
Size: 107 122 71 631 605 6912 1525
Fragments arranged by size:
6912 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 9968 5792
Size: 4176 5797
BlpI GC'TnA_GC
Cuts at: 3708 3837 7390 3708
Size: 129 3553 6291
Fragments arranged by size:
6291 3553 129
Bme1580I G_kGCm'C
Cuts at: 1790 5055 5517 8034 9190 1790
Size: 3265 462 2517 1156 2573
Fragments arranged by size:
3265 2573 2517 1156 462
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9973
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3638 4120 6488
Size: 416 53 83 399 24 2221 482 2368
Cuts at: 6488 7704 8662 442
Size: 1216 958 1753
Fragments arranged by size:
2368 2221 1753 1216 958 482 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3719 1056
Size: 2663 7310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4639 4639
Size: 9973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3793 4428 4734 4856 5299 5832 6516 8633
Size: 2554 635 306 122 443 533 684 2117
Cuts at: 8633 1239
Size: 2579
Fragments arranged by size:
2579 2554 2117 684 635 533 443 306
122
Bpu10I CC'TnA_GC
Cuts at: 6450 6450
Size: 9973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5143 5286 6930 7067 8098 8963 9261 5143
Size: 143 1644 137 1031 865 298 5855
Fragments arranged by size:
5855 1644 1031 865 298 143 137
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3750 5118 5801 8636 882
Size: 617 2251 1368 683 2835 2219
Fragments arranged by size:
2835 2251 2219 1368 683 617
BsaAI yAC'GTr
Cuts at: 493 1204 5104 7764 493
Size: 711 3900 2660 2702
Fragments arranged by size:
3900 2702 2660 711
BsaBI GATnn'nnATC
Cuts at: 3486 3972 4989 5797 9967 3486
Size: 486 1017 808 4170 3492
Fragments arranged by size:
4170 3492 1017 808 486
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7780 8162 8797 275
Size: 53 83 186 936 6247 382 635 1451
Fragments arranged by size:
6247 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2982 4576 5485 8406 8804 9078 9225 9636 2982
Size: 1594 909 2921 398 274 147 411 3319
Fragments arranged by size:
3319 2921 1594 909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3249 3279 3438 3468 4639
Size: 30 165 30 1846 30 159 30 1171
Cuts at: 4639 4669 4837 4867 5079 5109 5297 5327 6232
Size: 30 168 30 212 30 188 30 905
Cuts at: 6232 6262 6514 6544 1178
Size: 30 252 30 4607
Fragments arranged by size:
4607 1846 1171 905 252 212 188 168
165 159 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2476 4612 4853 6240 6243 7162 1186
Size: 1290 2136 241 1387 3 919 3997
Fragments arranged by size:
3997 2136 1387 1290 919 241 3
BseYI C'CCAG_C
Cuts at: 3227 4780 4849 5493 6276 9176 9672 3227
Size: 1553 69 644 783 2900 496 3528
Fragments arranged by size:
3528 2900 1553 783 644 496 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6069 6624 1163
Size: 573 4333 555 4512
Fragments arranged by size:
4512 4333 573 555
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7423 8184 9212 1540
Size: 275 186 5422 761 1028 2301
Fragments arranged by size:
5422 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5878 6331 8034 8119 9190 5878
Size: 453 1703 85 1071 6661
Fragments arranged by size:
6661 1703 1071 453 85
BsmI GAATG_Cn'
Cuts at: 4635 7484 7577 4635
Size: 2849 93 7031
Fragments arranged by size:
7031 2849 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2820 3993 6309 2820
Size: 1173 2316 6484
Fragments arranged by size:
6484 2316 1173
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 3635 4845 5055 5517 5878 6331
Size: 399 266 1579 1210 210 462 361 453
Cuts at: 6331 7292 8034 8119 9190 1391
Size: 961 742 85 1071 2174
Fragments arranged by size:
2174 1579 1210 1071 961 742 462 453
399 361 266 210 85
BspEI T'CCGG_A
Cuts at: 2982 9636 2982
Size: 6654 3319
BspHI T'CATG_A
Cuts at: 3205 4636 7862 9592 3205
Size: 1431 3226 1730 3586
Fragments arranged by size:
3586 3226 1730 1431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5591 7328 844
Size: 4747 1737 3489
Fragments arranged by size:
4747 3489 1737
BsrBI CCG'CTC
Cuts at: 1394 1823 3836 7860 1394
Size: 429 2013 4024 3507
Fragments arranged by size:
4024 3507 2013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2202 2977 5748 6090 6257 6901 8468
Size: 1183 954 775 2771 342 167 644 1567
Cuts at: 8468 8642 65
Size: 174 1396
Fragments arranged by size:
2771 1567 1396 1183 954 775 644 342
174 167
BsrFI r'CCGG_y
Cuts at: 1700 1810 2901 8617 8804 1700
Size: 110 1091 5716 187 2869
Fragments arranged by size:
5716 2869 1091 187 110
BsrGI T'GTAC_A
Cuts at: 96 3615 3724 5937 96
Size: 3519 109 2213 4132
Fragments arranged by size:
4132 3519 2213 109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9973
BssSI C'ACGA_G
Cuts at: 3996 8033 9045 3996
Size: 4037 1012 4924
Fragments arranged by size:
4924 4037 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6092 794
Size: 5298 4675
BstBI TT'CG_AA
Cuts at: 3649 3811 8777 3649
Size: 162 4966 4845
Fragments arranged by size:
4966 4845 162
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9973
BstYI r'GATC_y
Cuts at: 1972 4960 4984 5734 5792 5943 7219 7303 7670
Size: 2988 24 750 58 151 1276 84 367
Cuts at: 7670 8055 8072 9524 9610 9622 9968 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2988 1977 1452 1276 750 385 367 346
151 86 84 58 24 17 12
BstZ17I GTA'TAC
Cuts at: 3732 3732
Size: 9973
BtgI C'CryG_G
Cuts at: 513 1065 2004 2474 5620 7075 513
Size: 552 939 470 3146 1455 3411
Fragments arranged by size:
3411 3146 1455 939 552 470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9234
Fragments arranged by size:
9234 683 56
BtsI GCAGTG_nn'
Cuts at: 5696 7043 7489 8283 8303 5696
Size: 1347 446 794 20 7366
Fragments arranged by size:
7366 1347 794 446 20
ClaI AT'CG_AT
Cuts at: 1554 6544 1554
Size: 4990 4983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9938
DraI TTT'AAA
Cuts at: 7277 7277
Size: 9973
DraIII CAC_nnn'GTG
Cuts at: 6335 7121 6335
Size: 786 9187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8980 809
Size: 507 7664 1802
Fragments arranged by size:
7664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2392 2432 5276
Size: 54 1425 50 275 18 562 40 2844
Cuts at: 5276 6867 7420 8309 9938 8
Size: 1591 553 889 1629 43
Fragments arranged by size:
2844 1629 1591 1425 889 562 553 275
54 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 7420 1537
Size: 275 5608 4090
Fragments arranged by size:
5608 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2991 3117 5325 5361 5436 5721 6828 7903
Size: 1638 126 2208 36 75 285 1107 1075
Cuts at: 7903 1353
Size: 3423
Fragments arranged by size:
3423 2208 1638 1107 1075 285 126 75
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3768 4397 5416 8560 8934 9080
Size: 1064 138 2179 629 1019 3144 374 146
Cuts at: 9080 387
Size: 1280
Fragments arranged by size:
3144 2179 1280 1064 1019 629 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2184 3793 4428 4734 4856 5299 5490
Size: 393 552 1609 635 306 122 443 191
Cuts at: 5490 5832 5853 5990 6000 6033 6204 6516 6807
Size: 342 21 137 10 33 171 312 291
Cuts at: 6807 7112 8036 8633 9420 9688 1239
Size: 305 924 597 787 268 1524
Fragments arranged by size:
1609 1524 924 787 635 597 552 443
393 342 312 306 305 291 268 191
171 137 122 33 21 10
EcoNI CCTnn'n_nnAGG
Cuts at: 5508 5508
Size: 9973
EcoO109I rG'GnC_Cy
Cuts at: 4521 5513 4521
Size: 992 8981
EcoRI G'AATT_C
Cuts at: 6367 7282 7660 6367
Size: 915 378 8680
Fragments arranged by size:
8680 915 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3364 3437 3364
Size: 73 9900
FspI TGC'GCA
Cuts at: 8479 8479
Size: 9973
HaeII r_GCGC'y
Cuts at: 1678 9120 1678
Size: 7442 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2909 2941 3672
Size: 32 135 32 902 32 403 32 731
Cuts at: 3672 3704 3815 3847 4183 4215 4412 4444 5297
Size: 32 111 32 336 32 197 32 853
Cuts at: 5297 5329 7054 7086 7130 7160 7162 7192 8614
Size: 32 1725 32 44 30 2 30 1422
Cuts at: 8614 8646 8688 8720 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1725 1422 902 853 731 403 336
197 135 111 44 42 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2203 2410 7317 7563 669
Size: 988 281 265 207 4907 246 3079
Fragments arranged by size:
4907 3079 988 281 265 246 207
HindIII A'AGCT_T
Cuts at: 748 4740 748
Size: 3992 5981
HpaI GTT'AAC
Cuts at: 2203 2410 7563 2203
Size: 207 5153 4613
Fragments arranged by size:
5153 4613 207
KpnI G_GTAC'C
Cuts at: 7298 7298
Size: 9973
MfeI C'AATT_G
Cuts at: 4137 7572 4137
Size: 3435 6538
MluI A'CGCG_T
Cuts at: 9849 9849
Size: 9973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3277 4988 7191 7216 9087 9271 1305
Size: 1972 1711 2203 25 1871 184 2007
Fragments arranged by size:
2203 2007 1972 1871 1711 184 25
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6869 10
Size: 54 1425 343 5037 3114
Fragments arranged by size:
5037 3114 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 3045 3210 7931 8290 8449 518
Size: 648 1368 511 165 4721 359 159 2042
Fragments arranged by size:
4721 2042 1368 648 511 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2162 2476 2923 4357 5633 5644 6043 6827
Size: 556 314 447 1434 1276 11 399 784
Cuts at: 6827 7389 8066 9214 9459 1606
Size: 562 677 1148 245 2120
Fragments arranged by size:
2120 1434 1276 1148 784 677 562 556
447 399 314 245 11
NcoI C'CATG_G
Cuts at: 513 1065 2004 5620 7075 513
Size: 552 939 3616 1455 3411
Fragments arranged by size:
3616 3411 1455 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9973
NheI G'CTAG_C
Cuts at: 1052 3715 1052
Size: 2663 7310
NotI GC'GGCC_GC
Cuts at: 7420 7420
Size: 9973
NsiI A_TGCA'T
Cuts at: 4635 4635
Size: 9973
NspI r_CATG'y
Cuts at: 1132 1229 2234 3044 4079 4958 7331 8876 1132
Size: 97 1005 810 1035 879 2373 1545 2229
Fragments arranged by size:
2373 2229 1545 1035 1005 879 810 97
PasI CC'CwG_GG
Cuts at: 1348 5048 1348
Size: 3700 6273
PciI A'CATG_T
Cuts at: 8872 8872
Size: 9973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2390 2972 3182 5456 6500 1108
Size: 1282 582 210 2274 1044 4581
Fragments arranged by size:
4581 2274 1282 1044 582 210
PfoI T'CCnGG_A
Cuts at: 2056 2543 7213 2056
Size: 487 4670 4816
Fragments arranged by size:
4816 4670 487
PmeI GTTT'AAAC
Cuts at: 7277 7277
Size: 9973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5079 5111 8042 8074 9555 9587 5079
Size: 32 2931 32 1481 32 5465
Fragments arranged by size:
5465 2931 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9973
PsiI TTA'TAA
Cuts at: 7543 7543
Size: 9973
PspOMI G'GGCC_C
Cuts at: 5513 5513
Size: 9973
PspXI vC'TCGA_Gb
Cuts at: 5129 5129
Size: 9973
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3300 3332 4885 4917 3300
Size: 32 1553 32 8356
Fragments arranged by size:
8356 1553 32 32
PstI C_TGCA'G
Cuts at: 830 3337 5527 5632 7325 830
Size: 2507 2190 105 1693 3478
Fragments arranged by size:
3478 2507 2190 1693 105
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9973
PvuII CAG'CTG
Cuts at: 2162 4357 5633 6043 6827 2162
Size: 2195 1276 410 784 5308
Fragments arranged by size:
5308 2195 1276 784 410
SacII CC_GC'GG
Cuts at: 2477 2477
Size: 9973
SalI G'TCGA_C
Cuts at: 1936 7315 1936
Size: 5379 4594
SapI GCTCTTCn'nnn_
Cuts at: 5361 5436 5361
Size: 75 9898
SbfI CC_TGCA'GG
Cuts at: 7325 7325
Size: 9973
ScaI AGT'ACT
Cuts at: 4903 8221 9933 4903
Size: 3318 1712 4943
Fragments arranged by size:
4943 3318 1712
SfcI C'TryA_G
Cuts at: 826 1045 2174 3333 5523 5628 7321 8456 9137
Size: 219 1129 1159 2190 105 1693 1135 681
Cuts at: 9137 9328 826
Size: 191 1471
Fragments arranged by size:
2190 1693 1471 1159 1135 1129 681 219
191 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9973
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9973
SmaI CCC'GGG
Cuts at: 1872 7300 1872
Size: 5428 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5129 5158 5301 6945 7082 8077
Size: 197 926 3186 29 143 1644 137 995
Cuts at: 8077 8978 9240 820
Size: 901 262 1553
Fragments arranged by size:
3186 1644 1553 995 926 901 262 197
143 137 29
SnaBI TAC'GTA
Cuts at: 493 5104 7764 493
Size: 4611 2660 2702
Fragments arranged by size:
4611 2702 2660
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9973
SphI G_CATG'C
Cuts at: 1132 3044 7331 1132
Size: 1912 4287 3774
Fragments arranged by size:
4287 3774 1912
SspI AAT'ATT
Cuts at: 5 52 2635 3303 5552 6029 7897 5
Size: 47 2583 668 2249 477 1868 2081
Fragments arranged by size:
2583 2249 2081 1868 668 477 47
StuI AGG'CCT
Cuts at: 1517 2887 3904 1517
Size: 1370 1017 7586
Fragments arranged by size:
7586 1370 1017
StyI C'CwwG_G
Cuts at: 513 1065 2004 3339 5581 5620 7075 7412 513
Size: 552 939 1335 2242 39 1455 337 3074
Fragments arranged by size:
3074 2242 1455 1335 939 552 337 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2202 5061 5982 6072 8000 8017 8170
Size: 1255 622 2859 921 90 1928 17 153
Cuts at: 8170 8355 8800 325
Size: 185 445 1498
Fragments arranged by size:
2859 1928 1498 1255 921 622 445 185
153 90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3615 3724 4901
Size: 274 80 33 51 1443 1638 109 1177
Cuts at: 4901 5758 5937 8219 9931 96
Size: 857 179 2282 1712 138
Fragments arranged by size:
2282 1712 1638 1443 1177 857 274 179
138 109 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3249 3281 3438 3470 4837 4869 6232 6264 7109
Size: 32 157 32 1367 32 1363 32 845
Cuts at: 7109 7141 9933 9965 3249
Size: 32 2792 32 3257
Fragments arranged by size:
3257 2792 1367 1363 845 157 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9973
XbaI T'CTAG_A
Cuts at: 3319 5594 7309 3319
Size: 2275 1715 5983
Fragments arranged by size:
5983 2275 1715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3235 3946 4022 3235
Size: 711 76 9186
Fragments arranged by size:
9186 711 76
XhoI C'TCGA_G
Cuts at: 1943 5129 1943
Size: 3186 6787
XmaI C'CCGG_G
Cuts at: 1870 7298 1870
Size: 5428 4545
XmnI GAAnn'nnTTC
Cuts at: 4335 5912 8102 4335
Size: 1577 2190 6206
Fragments arranged by size:
6206 2190 1577
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7781 276
Size: 53 83 186 936 6247 2468
Fragments arranged by size:
6247 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI
BstXI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI RsrII SacI
SanDI SexAI SfiI SfoI SrfI SwaI