Restriction map for
pFN21AB0045
(Circular) (Six-base) MAPSORT of: pFN21AB0045.seq Check: 3576 from: 1 to: 9004
pFN21AB0045 9004 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6157 6157
Size: 9004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6814 278
Size: 53 83 186 936 5278 2468
Fragments arranged by size:
5278 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3880 6347 1937
Size: 1943 2467 4594
Fragments arranged by size:
4594 2467 1943
Acc65I G'GTAC_C
Cuts at: 4223 6325 4223
Size: 2102 6902
AclI AA'CG_TT
Cuts at: 1563 2644 7131 7504 1563
Size: 1081 4487 373 3063
Fragments arranged by size:
4487 3063 1081 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4110 5037 6153 7067 8451 8719 1632
Size: 2478 927 1116 914 1384 268 1917
Fragments arranged by size:
2478 1917 1384 1116 927 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8807
AflIII A'CryG_T
Cuts at: 1203 3875 5069 7903 8880 1203
Size: 2672 1194 2834 977 1327
Fragments arranged by size:
2834 2672 1327 1194 977
AgeI A'CCGG_T
Cuts at: 7835 7835
Size: 9004
AhdI GACnn_n'nnGTC
Cuts at: 7733 7733
Size: 9004
AleI CACnn'nnGTG
Cuts at: 1166 2623 4994 1166
Size: 1457 2371 5176
Fragments arranged by size:
5176 2371 1457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2910 1184
Size: 1726 7278
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5450 5482 5450
Size: 32 8972
AlwNI CAG_nnn'CTG
Cuts at: 2141 2639 2816 2819 4355 6747 8319 2141
Size: 498 177 3 1536 2392 1572 2826
Fragments arranged by size:
2826 2392 1572 1536 498 177 3
ApaLI G'TGCA_C
Cuts at: 2895 7061 8217 2895
Size: 4166 1156 3682
Fragments arranged by size:
4166 3682 1156
ApoI r'AATT_y
Cuts at: 1493 2413 2737 2985 3013 3179 3289 3581 4031
Size: 920 324 248 28 166 110 292 450
Cuts at: 4031 4244 4589 4645 4652 4730 4757 5287 5311
Size: 213 345 56 7 78 27 530 24
Cuts at: 5311 6085 6136 6313 6539 6691 8800 1493
Size: 774 51 177 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 774 530 450 345 324
292 248 226 213 177 166 152 110
78 56 51 28 27 24 7
AseI AT'TA_AT
Cuts at: 160 1032 7558 160
Size: 872 6526 1606
Fragments arranged by size:
6526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9004
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 4430 6175 6329 1870
Size: 73 68 2419 1745 154 4545
Fragments arranged by size:
4545 2419 1745 154 73 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4944 4977 5670 5703 4944
Size: 33 693 33 8245
Fragments arranged by size:
8245 693 33 33
BamHI G'GATC_C
Cuts at: 5780 6334 6701 5780
Size: 554 367 8083
Fragments arranged by size:
8083 554 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2857 4223 5873 6325 7780
Size: 325 217 627 1070 1366 1650 452 1455
Cuts at: 7780 618
Size: 1842
Fragments arranged by size:
1842 1650 1455 1366 1070 627 452 325
217
BanII G_rGCy'C
Cuts at: 1391 2285 4150 5404 6323 1391
Size: 894 1865 1254 919 4072
Fragments arranged by size:
4072 1865 1254 919 894
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2875 3194 4311 928
Size: 971 976 319 1117 5621
Fragments arranged by size:
5621 1117 976 971 319
BbvCI CC'TCA_GC
Cuts at: 5809 6157 5809
Size: 348 8656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7195 7229 1903
Size: 34 5258 34 3678
Fragments arranged by size:
5258 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6896 8106 6896
Size: 1210 7794
BclI T'GATC_A
Cuts at: 1548 5422 1548
Size: 3874 5130
BfrBI ATG'CAT
Cuts at: 3912 3912
Size: 9004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2164 7615 136
Size: 107 122 71 631 605 492 5451 1525
Fragments arranged by size:
5451 1525 631 605 492 122 107 71
BglII A'GATC_T
Cuts at: 8999 8999
Size: 9004
BlpI GC'TnA_GC
Cuts at: 2883 3759 6421 2883
Size: 876 2662 5466
Fragments arranged by size:
5466 2662 876
Bme1580I G_kGCm'C
Cuts at: 1790 2899 7065 8221 1790
Size: 1109 4166 1156 2573
Fragments arranged by size:
4166 2573 1156 1109
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3773 4847 6735
Size: 416 53 83 399 24 2356 1074 1888
Cuts at: 6735 7693 442
Size: 958 1753
Fragments arranged by size:
2356 1888 1753 1074 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 5164 2299
Size: 2865 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3345 3410 3869 4416 6186 7664 1239
Size: 2106 65 459 547 1770 1478 2579
Fragments arranged by size:
2579 2106 1770 1478 547 459 65
Bpu10I CC'TnA_GC
Cuts at: 2313 5809 6157 2313
Size: 3496 348 5160
Fragments arranged by size:
5160 3496 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3504 5628 7129 7994 8292 3504
Size: 2124 1501 865 298 4216
Fragments arranged by size:
4216 2124 1501 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7667 882
Size: 617 6168 2219
Fragments arranged by size:
6168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6795 493
Size: 711 5591 2702
Fragments arranged by size:
5591 2702 711
BsaBI GATnn'nnATC
Cuts at: 3041 5908 8998 3041
Size: 2867 3090 3047
Fragments arranged by size:
3090 3047 2867
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6811 7193 7828 275
Size: 53 83 186 936 5278 382 635 1451
Fragments arranged by size:
5278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2052 7437 7835 8109 8256 8667 2052
Size: 5385 398 274 147 411 2389
Fragments arranged by size:
5385 2389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3481 3511 4963 4993 1178
Size: 30 165 30 2078 30 1452 30 5189
Fragments arranged by size:
5189 2078 1452 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2037 2291 2997 3009 3407 3410 4567 1186
Size: 851 254 706 12 398 3 1157 5623
Fragments arranged by size:
5623 1157 851 706 398 254 12 3
BseYI C'CCAG_C
Cuts at: 3851 4440 6162 8207 8703 3851
Size: 589 1722 2045 496 4152
Fragments arranged by size:
4152 2045 1722 589 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3235 4192 4484 5451 5934 1163
Size: 573 1499 957 292 967 483 4233
Fragments arranged by size:
4233 1499 967 957 573 483 292
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6454 7215 8243 1540
Size: 275 186 4453 761 1028 2301
Fragments arranged by size:
4453 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2285 2899 5404 7065 7150 8221 2285
Size: 614 2505 1661 85 1071 3068
Fragments arranged by size:
3068 2505 1661 1071 614 85
BsmI GAATG_Cn'
Cuts at: 2118 2468 2693 6515 6608 2118
Size: 350 225 3822 93 4514
Fragments arranged by size:
4514 3822 350 225 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3346 3522 3346
Size: 176 8828
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2899 4150 5404 6323 7065 7150
Size: 399 495 614 1251 1254 919 742 85
Cuts at: 7150 8221 1391
Size: 1071 2174
Fragments arranged by size:
2174 1254 1251 1071 919 742 614 495
399 85
BspEI T'CCGG_A
Cuts at: 2052 8667 2052
Size: 6615 2389
BspHI T'CATG_A
Cuts at: 6893 8623 6893
Size: 1730 7274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4363 4928 6157 6359 844
Size: 3519 565 1229 202 3489
Fragments arranged by size:
3519 3489 1229 565 202
BsrBI CCG'CTC
Cuts at: 1394 1823 6891 1394
Size: 429 5068 3507
Fragments arranged by size:
5068 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2612 7499 7673 65
Size: 1183 1364 4887 174 1396
Fragments arranged by size:
4887 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7648 7835 1700
Size: 110 5838 187 2869
Fragments arranged by size:
5838 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9004
BssSI C'ACGA_G
Cuts at: 7064 8076 7064
Size: 1012 7992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2816 5819 5837 794
Size: 2022 3003 18 3961
Fragments arranged by size:
3961 3003 2022 18
BstBI TT'CG_AA
Cuts at: 4562 7808 4562
Size: 3246 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9004
BstXI CCAn_nnnn'nTGG
Cuts at: 2625 4492 2625
Size: 1867 7137
BstYI r'GATC_y
Cuts at: 1972 2049 4262 5780 5903 6334 6701 7086 7103
Size: 77 2213 1518 123 431 367 385 17
Cuts at: 7103 8555 8641 8653 8999 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2213 1977 1518 1452 431 385 367 346
123 86 77 17 12
Bsu36I CC'TnA_GG
Cuts at: 4228 4228
Size: 9004
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7513
Fragments arranged by size:
7513 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3717 487
Size: 683 56 2491 5774
Fragments arranged by size:
5774 2491 683 56
BtsI GCAGTG_nn'
Cuts at: 2667 3452 3872 5604 6520 7314 7334 2667
Size: 785 420 1732 916 794 20 4337
Fragments arranged by size:
4337 1732 916 794 785 420 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8969
DraI TTT'AAA
Cuts at: 3154 3580 3846 4036 4534 4651 4729 4756 6308
Size: 426 266 190 498 117 78 27 1552
Cuts at: 6308 3154
Size: 5850
Fragments arranged by size:
5850 1552 498 426 266 190 117 78
27
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8011 809
Size: 507 6695 1802
Fragments arranged by size:
6695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 6451 7340
Size: 54 1425 50 275 18 407 4214 889
Cuts at: 7340 8969 8
Size: 1629 43
Fragments arranged by size:
4214 1629 1425 889 407 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6451 1537
Size: 275 4639 4090
Fragments arranged by size:
4639 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2400 4615 5184 5247 5533 6250 6934 1353
Size: 1047 2215 569 63 286 717 684 3423
Fragments arranged by size:
3423 2215 1047 717 684 569 286 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7591 7965 8111 387
Size: 1064 138 6002 374 146 1280
Fragments arranged by size:
6002 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2283 5402 2283
Size: 3119 5885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3345 3410 3869 4110 4416 5037 6153
Size: 393 1713 65 459 241 306 621 1116
Cuts at: 6153 6186 7067 7664 8451 8719 1239
Size: 33 881 597 787 268 1524
Fragments arranged by size:
1713 1524 1116 881 787 621 597 459
393 306 268 241 65 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 4395 2501
Size: 1894 7110
EcoRI G'AATT_C
Cuts at: 2737 2985 3013 3179 5287 5311 6313 6691 2737
Size: 248 28 166 2108 24 1002 378 5050
Fragments arranged by size:
5050 2108 1002 378 248 166 28 24
EcoRV GAT'ATC
Cuts at: 3043 3043
Size: 9004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5035 5035
Size: 9004
FspI TGC'GCA
Cuts at: 7510 7510
Size: 9004
HaeII r_GCGC'y
Cuts at: 1678 8151 1678
Size: 6473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2531 2539 2563 2942
Size: 32 135 32 935 24 8 24 379
Cuts at: 2942 2974 4685 4717 5348 5380 7645 7677 7719
Size: 32 1711 32 631 32 2265 32 42
Cuts at: 7719 7751 1373
Size: 32 2626
Fragments arranged by size:
2626 2265 1711 935 631 379 135 42
32 32 32 32 32 32 32 24
24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2382 5267 6348 6594 669
Size: 988 281 444 2885 1081 246 3079
Fragments arranged by size:
3079 2885 1081 988 444 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9004
HpaI GTT'AAC
Cuts at: 2382 6594 2382
Size: 4212 4792
KpnI G_GTAC'C
Cuts at: 4227 6329 4227
Size: 2102 6902
MfeI C'AATT_G
Cuts at: 2475 6603 2475
Size: 4128 4876
MluI A'CGCG_T
Cuts at: 8880 8880
Size: 9004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2008 2406 8118 8302 1305
Size: 703 398 5712 184 2007
Fragments arranged by size:
5712 2007 703 398 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2239 10
Size: 54 1425 343 407 6775
Fragments arranged by size:
6775 1425 407 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2535 2623 3865 4994 6962 7321 7480
Size: 648 1369 88 1242 1129 1968 359 159
Cuts at: 7480 518
Size: 2042
Fragments arranged by size:
2042 1968 1369 1242 1129 648 359 159
88
MspA1I CmG'CkG
Cuts at: 1606 2205 2816 4206 5420 5684 6420 7097 8245
Size: 599 611 1390 1214 264 736 677 1148
Cuts at: 8245 8490 1606
Size: 245 2120
Fragments arranged by size:
2120 1390 1214 1148 736 677 611 599
264 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7513
Fragments arranged by size:
7513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9004
NotI GC'GGCC_GC
Cuts at: 6451 6451
Size: 9004
NsiI A_TGCA'T
Cuts at: 3914 3914
Size: 9004
NspI r_CATG'y
Cuts at: 1132 1229 3879 4500 5073 6362 7907 1132
Size: 97 2650 621 573 1289 1545 2229
Fragments arranged by size:
2650 2229 1545 1289 621 573 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9004
PciI A'CATG_T
Cuts at: 3875 5069 7903 3875
Size: 1194 2834 4976
Fragments arranged by size:
4976 2834 1194
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5834 5858 5900 1108
Size: 4726 24 42 4212
Fragments arranged by size:
4726 4212 42 24
PfoI T'CCnGG_A
Cuts at: 2785 5707 2785
Size: 2922 6082
PmeI GTTT'AAAC
Cuts at: 6308 6308
Size: 9004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7073 7105 8586 8618 7073
Size: 32 1481 32 7459
Fragments arranged by size:
7459 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9004
PsiI TTA'TAA
Cuts at: 3713 6574 3713
Size: 2861 6143
PstI C_TGCA'G
Cuts at: 830 3307 4939 5779 6154 6356 830
Size: 2477 1632 840 375 202 3478
Fragments arranged by size:
3478 2477 1632 840 375 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9004
PvuII CAG'CTG
Cuts at: 2205 2816 4206 5420 5684 2205
Size: 611 1390 1214 264 5525
Fragments arranged by size:
5525 1390 1214 611 264
SacI G_AGCT'C
Cuts at: 2285 5404 2285
Size: 3119 5885
SalI G'TCGA_C
Cuts at: 1936 6346 1936
Size: 4410 4594
SapI GCTCTTCn'nnn_
Cuts at: 5247 5247
Size: 9004
SbfI CC_TGCA'GG
Cuts at: 6356 6356
Size: 9004
ScaI AGT'ACT
Cuts at: 2356 3220 3697 5413 7252 8964 2356
Size: 864 477 1716 1839 1712 2396
Fragments arranged by size:
2396 1839 1716 1712 864 477
SfcI C'TryA_G
Cuts at: 826 1045 3303 4935 5415 5775 6150 6352 7487
Size: 219 2258 1632 480 360 375 202 1135
Cuts at: 7487 8168 8359 826
Size: 681 191 1471
Fragments arranged by size:
2258 1632 1471 1135 681 480 375 360
219 202 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9004
SmaI CCC'GGG
Cuts at: 1872 6331 1872
Size: 4459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3519 5643 7108 8009 8271 820
Size: 197 926 1576 2124 1465 901 262 1553
Fragments arranged by size:
2124 1576 1553 1465 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6795 493
Size: 6302 2702
SpeI A'CTAG_T
Cuts at: 152 2351 5408 152
Size: 2199 3057 3748
Fragments arranged by size:
3748 3057 2199
SphI G_CATG'C
Cuts at: 1132 6362 1132
Size: 5230 3774
SspI AAT'ATT
Cuts at: 5 52 2152 2290 2937 6928 5
Size: 47 2100 138 647 3991 2081
Fragments arranged by size:
3991 2100 2081 647 138 47
StuI AGG'CCT
Cuts at: 1517 2389 1517
Size: 872 8132
StyI C'CwwG_G
Cuts at: 513 1065 2004 5026 6443 513
Size: 552 939 3022 1417 3074
Fragments arranged by size:
3074 3022 1417 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3520 3955 7031 7048 7201 7386 7831
Size: 1255 1940 435 3076 17 153 185 445
Cuts at: 7831 325
Size: 1498
Fragments arranged by size:
3076 1940 1498 1255 445 435 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2354 3218 3619
Size: 274 80 33 51 1443 377 864 401
Cuts at: 3619 3695 5411 5480 7250 8962 96
Size: 76 1716 69 1770 1712 138
Fragments arranged by size:
1770 1716 1712 1443 864 401 377 274
138 80 76 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3553 3585 4122 4154 4963 4995 8964 8996 3553
Size: 32 537 32 809 32 3969 32 3561
Fragments arranged by size:
3969 3561 809 537 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5865 5971 1316
Size: 4549 106 4349
Fragments arranged by size:
4549 4349 106
XbaI T'CTAG_A
Cuts at: 6340 6340
Size: 9004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5542 5542
Size: 9004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9004
XmaI C'CCGG_G
Cuts at: 1870 6329 1870
Size: 4459 4545
XmnI GAAnn'nnTTC
Cuts at: 2719 7133 2719
Size: 4414 4590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6812 276
Size: 53 83 186 936 5278 2468
Fragments arranged by size:
5278 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BsiWI BstZ17I DraIII
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI