Restriction map for
pFN21AB0038
(Circular) (Six-base) MAPSORT of: pFN21AB0038.seq Check: 9529 from: 1 to: 5872
pFN21AB0038 5872 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2435 2435
Size: 5872
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3215 1937
Size: 1278 4594
Acc65I G'GTAC_C
Cuts at: 2546 3193 2546
Size: 647 5225
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 3935 5319 5587 1632
Size: 443 1860 1384 268 1917
Fragments arranged by size:
1917 1860 1384 443 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 3098 1166
Size: 1932 3940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2886 1184
Size: 1702 4170
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2018 2050 2018
Size: 32 5840
AlwNI CAG_nnn'CTG
Cuts at: 2344 2427 3615 5187 2344
Size: 83 1188 1572 3029
Fragments arranged by size:
3029 1572 1188 83
ApaI G_GGCC'C
Cuts at: 2365 2983 2365
Size: 618 5254
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 3181 3407 3559 5668 1493
Size: 1688 226 152 2109 1697
Fragments arranged by size:
2109 1697 1688 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2358 2364 2445 2919 3197 1870
Size: 73 415 6 81 474 278 4545
Fragments arranged by size:
4545 474 415 278 81 73 6
BamHI G'GATC_C
Cuts at: 2313 3012 3202 3569 2313
Size: 699 190 367 4616
Fragments arranged by size:
4616 699 367 190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2546 2897 2915 3193 4648
Size: 325 217 627 759 351 18 278 1455
Cuts at: 4648 618
Size: 1842
Fragments arranged by size:
1842 1455 759 627 351 325 278 217
18
BanII G_rGCy'C
Cuts at: 1391 2202 2365 2625 2983 3062 3191 1391
Size: 811 163 260 358 79 129 4072
Fragments arranged by size:
4072 811 358 260 163 129 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2767 928
Size: 971 868 4033
Fragments arranged by size:
4033 971 868
BbvCI CC'TCA_GC
Cuts at: 2811 2811
Size: 5872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 2806 3289 2806
Size: 483 5389
Bme1580I G_kGCm'C
Cuts at: 1790 2365 2902 2983 3933 5089 1790
Size: 575 537 81 950 1156 2573
Fragments arranged by size:
2573 1156 950 575 537 81
BmgBI CAC'GTC
Cuts at: 1318 3100 1318
Size: 1782 4090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4561 442
Size: 416 53 83 399 24 2186 958 1753
Fragments arranged by size:
2186 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2696
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2900 3076 3098 4532 1239
Size: 1661 176 22 1434 2579
Fragments arranged by size:
2579 1661 1434 176 22
Bpu10I CC'TnA_GC
Cuts at: 2811 2935 2811
Size: 124 5748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2666 3997 4862 5160 2666
Size: 1331 865 298 3378
Fragments arranged by size:
3378 1331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2454 4535 882
Size: 617 955 2081 2219
Fragments arranged by size:
2219 2081 955 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2134 2310 4305 4703 4977 5124 5535 2134
Size: 176 1995 398 274 147 411 2471
Fragments arranged by size:
2471 1995 411 398 274 176 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 2333 2363 3035
Size: 30 165 30 615 30 285 30 672
Cuts at: 3035 3065 1178
Size: 30 3985
Fragments arranged by size:
3985 672 615 285 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 1186
Size: 1048 4824
BseYI C'CCAG_C
Cuts at: 2570 2657 2945 5075 5571 2570
Size: 87 288 2130 496 2871
Fragments arranged by size:
2871 2130 496 288 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 1163
Size: 573 1065 4234
Fragments arranged by size:
4234 1065 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 3322 4083 5111 1540
Size: 275 186 42 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2202 2625 3933 4018 5089 2202
Size: 423 1308 85 1071 2985
Fragments arranged by size:
2985 1308 1071 423 85
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2365 2625 2902 2983 3062 3191
Size: 399 412 163 260 277 81 79 129
Cuts at: 3191 3933 4018 5089 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 277 260 163
129 85 81 79
BspEI T'CCGG_A
Cuts at: 2310 5535 2310
Size: 3225 2647
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 3227 844
Size: 1591 792 3489
Fragments arranged by size:
3489 1591 792
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2331 2482 2518 2542 3759 1394
Size: 429 206 302 151 36 24 1217 3507
Fragments arranged by size:
3507 1217 429 302 206 151 36 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 4367 4541 65
Size: 1183 3119 174 1396
Fragments arranged by size:
3119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2663 2732 4516 4703 1700
Size: 110 853 69 1784 187 2869
Fragments arranged by size:
2869 1784 853 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 2090 3932 4944 2090
Size: 1842 1012 3018
Fragments arranged by size:
3018 1842 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2427 794
Size: 1633 4239
BstBI TT'CG_AA
Cuts at: 2287 2377 4676 2287
Size: 90 2299 3483
Fragments arranged by size:
3483 2299 90
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstXI CCAn_nnnn'nTGG
Cuts at: 2557 2953 3031 2557
Size: 396 78 5398
Fragments arranged by size:
5398 396 78
BstYI r'GATC_y
Cuts at: 1972 2313 2905 3012 3202 3569 3954 3971 5423
Size: 341 592 107 190 367 385 17 1452
Cuts at: 5423 5509 5521 5867 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 592 385 367 346 341 190
107 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2647 2647
Size: 5872
BtgI C'CryG_G
Cuts at: 513 1065 2004 2817 513
Size: 552 939 813 3568
Fragments arranged by size:
3568 939 813 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2989 487
Size: 683 56 1763 3370
Fragments arranged by size:
3370 1763 683 56
BtsI GCAGTG_nn'
Cuts at: 2789 3388 4182 4202 2789
Size: 599 794 20 4459
Fragments arranged by size:
4459 794 599 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2200 2235 529
Size: 35 1636 35 4166
Fragments arranged by size:
4166 1636 35 35
DraI TTT'AAA
Cuts at: 3160 3176 3160
Size: 16 5856
DraIII CAC_nnn'GTG
Cuts at: 2743 2743
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4879 809
Size: 507 3563 1802
Fragments arranged by size:
3563 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2326 2734
Size: 54 1425 50 275 18 210 286 408
Cuts at: 2734 2847 3021 3319 4208 5837 8
Size: 113 174 298 889 1629 43
Fragments arranged by size:
1629 1425 889 408 298 286 275 210
174 113 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 3319 1537
Size: 275 228 1279 4090
Fragments arranged by size:
4090 1279 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 3042 3802 1353
Size: 1689 760 3423
Fragments arranged by size:
3423 1689 760
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2623 2200
Size: 423 5449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2900 3076 3098 3935 4532 5319
Size: 393 443 825 176 22 837 597 787
Cuts at: 5319 5587 1239
Size: 268 1524
Fragments arranged by size:
1524 837 825 787 597 443 393 268
176 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2638 3080 2638
Size: 442 5430
EcoO109I rG'GnC_Cy
Cuts at: 2341 2371 2440 2603 2643 2939 2959 2979 2994
Size: 30 69 163 40 296 20 20 15
Cuts at: 2994 3037 2341
Size: 43 5176
Fragments arranged by size:
5176 296 163 69 43 40 30 20
20 15
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 2664 5019 1678
Size: 986 2355 2531
Fragments arranged by size:
2531 2355 986
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2333 2365 3035 3067 3089
Size: 32 135 32 761 32 670 32 22
Cuts at: 3089 3121 4513 4545 4587 4619 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 761 670 135 42 32 32
32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3216 3462 669
Size: 988 281 1278 246 3079
Fragments arranged by size:
3079 1278 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 2550 3197 2550
Size: 647 5225
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2950 4986 5170 1305
Size: 1645 2036 184 2007
Fragments arranged by size:
2036 2007 1645 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3023 10
Size: 54 1425 343 1191 2859
Fragments arranged by size:
2859 1425 1191 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 3830 4189 4348 518
Size: 648 1932 732 359 159 2042
Fragments arranged by size:
2042 1932 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2752 2810 3288 3965 5113 5358 1606
Size: 412 734 58 478 677 1148 245 2120
Fragments arranged by size:
2120 1148 734 677 478 412 245 58
NaeI GCC'GGC
Cuts at: 2665 2734 2665
Size: 69 5803
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4381
Fragments arranged by size:
4381 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NgoMIV G'CCGG_C
Cuts at: 2663 2732 2663
Size: 69 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 3230 4775 1132
Size: 97 2001 1545 2229
Fragments arranged by size:
2229 2001 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2983 1348
Size: 1635 4237
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 1108
Size: 1236 4636
PfoI T'CCnGG_A
Cuts at: 2148 2625 2877 3053 2148
Size: 477 252 176 4967
Fragments arranged by size:
4967 477 252 176
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2050 3941 3973 5454 5486 2018
Size: 32 1891 32 1481 32 2404
Fragments arranged by size:
2404 1891 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 3037 2341
Size: 696 5176
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PspOMI G'GGCC_C
Cuts at: 2361 2979 2361
Size: 618 5254
PstI C_TGCA'G
Cuts at: 830 3224 830
Size: 2394 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2752 2810 2752
Size: 58 5814
SacI G_AGCT'C
Cuts at: 2202 2625 2202
Size: 423 5449
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SanDI GG'GwC_CC
Cuts at: 3037 3037
Size: 5872
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SexAI A'CCwGG_T
Cuts at: 2775 2775
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 3220 4355 5036 5227 826
Size: 219 2175 1135 681 191 1471
Fragments arranged by size:
2175 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 2360 2366 2921 3199 1872
Size: 488 6 555 278 4545
Fragments arranged by size:
4545 555 488 278 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2681 3976 4877 5139 820
Size: 197 926 738 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 738 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2004 2259 3148 3311 513
Size: 552 939 255 889 163 3074
Fragments arranged by size:
3074 939 889 552 255 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2472 3899 3916 4069 4254 4699 325
Size: 1255 892 1427 17 153 185 445 1498
Fragments arranged by size:
1498 1427 1255 892 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 4118 5830
Size: 274 80 33 51 1443 141 2000 1712
Cuts at: 5830 96
Size: 138
Fragments arranged by size:
2000 1712 1443 274 141 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5832 5864 5832
Size: 32 5840
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2824 3016 2824
Size: 192 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 2358 2364 2919 3197 1870
Size: 488 6 555 278 4545
Fragments arranged by size:
4545 555 488 278 6
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SapI
SfiI SfoI SrfI SwaI