Restriction map for
pFN21AB0031
(Circular) (Six-base) MAPSORT of: pFN21AB0031.seq Check: 1402 from: 1 to: 7450
pFN21AB0031 7450 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5260 278
Size: 53 83 186 936 3724 2468
Fragments arranged by size:
3724 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4793 1937
Size: 2856 4594
Acc65I G'GTAC_C
Cuts at: 4771 4771
Size: 7450
AclI AA'CG_TT
Cuts at: 1563 4586 5577 5950 1563
Size: 3023 991 373 3063
Fragments arranged by size:
3063 3023 991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2930 3434 3716 4124 5513 6897 7165 1632
Size: 1298 504 282 408 1389 1384 268 1917
Fragments arranged by size:
1917 1389 1384 1298 504 408 282 268
AflII C'TTAA_G
Cuts at: 820 1017 2969 820
Size: 197 1952 5301
Fragments arranged by size:
5301 1952 197
AflIII A'CryG_T
Cuts at: 1203 3911 6349 7326 1203
Size: 2708 2438 977 1327
Fragments arranged by size:
2708 2438 1327 977
AgeI A'CCGG_T
Cuts at: 6281 6281
Size: 7450
AhdI GACnn_n'nnGTC
Cuts at: 3484 6179 3484
Size: 2695 4755
AleI CACnn'nnGTG
Cuts at: 1166 2564 1166
Size: 1398 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4604 1184
Size: 3420 4030
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2316 2348 2316
Size: 32 7418
AlwNI CAG_nnn'CTG
Cuts at: 3099 5193 6765 3099
Size: 2094 1572 3784
Fragments arranged by size:
3784 2094 1572
ApaLI G'TGCA_C
Cuts at: 5507 6663 5507
Size: 1156 6294
ApoI r'AATT_y
Cuts at: 1493 2070 2393 2502 2515 2974 3657 3883 3896
Size: 577 323 109 13 459 683 226 13
Cuts at: 3896 4595 4759 4985 5137 7246 1493
Size: 699 164 226 152 2109 1697
Fragments arranged by size:
2109 1697 699 683 577 459 323 226
226 164 152 109 13 13
AseI AT'TA_AT
Cuts at: 160 1032 3075 6004 160
Size: 872 2043 2929 1606
Fragments arranged by size:
2929 2043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7450
AvaI C'yCGr_G
Cuts at: 1870 1943 4512 4775 1870
Size: 73 2569 263 4545
Fragments arranged by size:
4545 2569 263 73
BamHI G'GATC_C
Cuts at: 4780 5147 4780
Size: 367 7083
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4771 6226 618
Size: 325 217 627 2984 1455 1842
Fragments arranged by size:
2984 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 4769 1391
Size: 627 2751 4072
Fragments arranged by size:
4072 2751 627
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2539 2577 4073 4142 4428 928
Size: 971 640 38 1496 69 286 3950
Fragments arranged by size:
3950 1496 971 640 286 69 38
BbvCI CC'TCA_GC
Cuts at: 2281 2281
Size: 7450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5641 5675 1903
Size: 34 3704 34 3678
Fragments arranged by size:
3704 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5342 6552 5342
Size: 1210 6240
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6061 136
Size: 107 122 71 631 605 4389 1525
Fragments arranged by size:
4389 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3409 7445 3409
Size: 4036 3414
BlpI GC'TnA_GC
Cuts at: 3498 4867 3498
Size: 1369 6081
Bme1580I G_kGCm'C
Cuts at: 1790 5511 6667 1790
Size: 3721 1156 2573
Fragments arranged by size:
3721 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3914 1318
Size: 2596 4854
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3175 5181 6139
Size: 416 53 83 399 24 1758 2006 958
Cuts at: 6139 442
Size: 1753
Fragments arranged by size:
2006 1758 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3120 4263 1056
Size: 2064 1143 4243
Fragments arranged by size:
4243 2064 1143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 3510 3540 6110 1239
Size: 950 1321 30 2570 2579
Fragments arranged by size:
2579 2570 1321 950 30
Bpu10I CC'TnA_GC
Cuts at: 2281 4700 2281
Size: 2419 5031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2050 2355 3263 4682 5575 6440 6738 2050
Size: 305 908 1419 893 865 298 2762
Fragments arranged by size:
2762 1419 908 893 865 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6113 882
Size: 617 4614 2219
Fragments arranged by size:
4614 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3920 5241 493
Size: 711 2716 1321 2702
Fragments arranged by size:
2716 2702 1321 711
BsaBI GATnn'nnATC
Cuts at: 2964 4285 7444 2964
Size: 1321 3159 2970
Fragments arranged by size:
3159 2970 1321
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5257 5639 6274 275
Size: 53 83 186 936 3724 382 635 1451
Fragments arranged by size:
3724 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5883 6281 6555 6702 7113 5883
Size: 398 274 147 411 6220
Fragments arranged by size:
6220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2235 2265 3885
Size: 30 165 30 624 30 178 30 1620
Cuts at: 3885 3915 1178
Size: 30 4713
Fragments arranged by size:
4713 1620 624 178 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2008 4679 1186
Size: 822 2671 3957
Fragments arranged by size:
3957 2671 822
BseYI C'CCAG_C
Cuts at: 6653 7149 6653
Size: 496 6954
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2787 4323 1163
Size: 573 1051 1536 4290
Fragments arranged by size:
4290 1536 1051 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4900 5661 6689 1540
Size: 275 186 2899 761 1028 2301
Fragments arranged by size:
2899 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2018 5511 5596 6667 2018
Size: 3493 85 1071 2801
Fragments arranged by size:
3493 2801 1071 85
BsmI GAATG_Cn'
Cuts at: 3331 4636 4961 5054 3331
Size: 1305 325 93 5727
Fragments arranged by size:
5727 1305 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 4769 5511 5596 6667 1391
Size: 399 228 2751 742 85 1071 2174
Fragments arranged by size:
2751 2174 1071 742 399 228 85
BspEI T'CCGG_A
Cuts at: 7113 7113
Size: 7450
BspHI T'CATG_A
Cuts at: 2494 2962 5339 7069 2494
Size: 468 2377 1730 2875
Fragments arranged by size:
2875 2377 1730 468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4805 844
Size: 3961 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5337 1394
Size: 429 3514 3507
Fragments arranged by size:
3514 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5945 6119 65
Size: 1183 4697 174 1396
Fragments arranged by size:
4697 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4180 6094 6281 1700
Size: 110 2370 1914 187 2869
Fragments arranged by size:
2869 2370 1914 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7450
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7450
BssSI C'ACGA_G
Cuts at: 5510 6522 5510
Size: 1012 6438
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7450
BstBI TT'CG_AA
Cuts at: 3661 6254 3661
Size: 2593 4857
BstEII G'GTnAC_C
Cuts at: 1184 4183 1184
Size: 2999 4451
BstYI r'GATC_y
Cuts at: 1972 3038 3409 4280 4492 4780 5147 5532 5549
Size: 1066 371 871 212 288 367 385 17
Cuts at: 5549 7001 7087 7099 7445 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1066 871 385 371 367 346
288 212 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3217 513
Size: 552 2152 4746
Fragments arranged by size:
4746 2152 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6711
Fragments arranged by size:
6711 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 4966 5760 5780 3217
Size: 1749 794 20 4887
Fragments arranged by size:
4887 1749 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7450
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3093 3128 529
Size: 35 2529 35 4851
Fragments arranged by size:
4851 2529 35 35
DraI TTT'AAA
Cuts at: 4754 4754
Size: 7450
DraIII CAC_nnn'GTG
Cuts at: 2140 2140
Size: 7450
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6457 809
Size: 507 5141 1802
Fragments arranged by size:
5141 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4897 5786 7415
Size: 54 1425 50 275 18 3067 889 1629
Cuts at: 7415 8
Size: 43
Fragments arranged by size:
3067 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4897 1537
Size: 275 3085 4090
Fragments arranged by size:
4090 3085 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2241 2643 2712 2754 3438 3537 3955
Size: 653 235 402 69 42 684 99 418
Cuts at: 3955 3966 4119 4143 5380 1353
Size: 11 153 24 1237 3423
Fragments arranged by size:
3423 1237 684 653 418 402 235 153
99 69 42 24 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 6037 6411 6557 387
Size: 1064 138 531 3917 374 146 1280
Fragments arranged by size:
3917 1280 1064 531 374 146 138
EcoICRI GAG'CTC
Cuts at: 2016 2016
Size: 7450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2930 3434 3510 3540 3716 4124
Size: 393 557 741 504 76 30 176 408
Cuts at: 4124 5513 6110 6897 7165 1239
Size: 1389 597 787 268 1524
Fragments arranged by size:
1524 1389 787 741 597 557 504 408
393 268 176 76 30
EcoRI G'AATT_C
Cuts at: 2070 3657 4759 5137 2070
Size: 1587 1102 378 4383
Fragments arranged by size:
4383 1587 1102 378
EcoRV GAT'ATC
Cuts at: 4093 4093
Size: 7450
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4049 4154 4715 4049
Size: 105 561 6784
Fragments arranged by size:
6784 561 105
FspI TGC'GCA
Cuts at: 5956 5956
Size: 7450
HaeII r_GCGC'y
Cuts at: 1678 6597 1678
Size: 4919 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2648 2680 3266
Size: 32 135 32 692 32 352 32 586
Cuts at: 3266 3298 3470 3502 3617 3649 4096 4128 4248
Size: 32 172 32 115 32 447 32 120
Cuts at: 4248 4280 6091 6123 6165 6197 1373
Size: 32 1811 32 42 32 2626
Fragments arranged by size:
2626 1811 692 586 447 352 172 135
120 115 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4169 4794 5040 669
Size: 988 281 2231 625 246 3079
Fragments arranged by size:
3079 2231 988 625 281 246
HindIII A'AGCT_T
Cuts at: 748 3940 4131 4697 748
Size: 3192 191 566 3501
Fragments arranged by size:
3501 3192 566 191
HpaI GTT'AAC
Cuts at: 5040 5040
Size: 7450
KpnI G_GTAC'C
Cuts at: 4775 4775
Size: 7450
MfeI C'AATT_G
Cuts at: 5049 5049
Size: 7450
MluI A'CGCG_T
Cuts at: 7326 7326
Size: 7450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3603 6564 6748 1305
Size: 2298 2961 184 2007
Fragments arranged by size:
2961 2298 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5628
Fragments arranged by size:
5628 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 3027 3966 5408 5767 5926 518
Size: 648 1398 463 939 1442 359 159 2042
Fragments arranged by size:
2042 1442 1398 939 648 463 359 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2921 3502 3718 4866 5543 6691 6936
Size: 635 680 581 216 1148 677 1148 245
Cuts at: 6936 1606
Size: 2120
Fragments arranged by size:
2120 1148 1148 680 677 635 581 245
216
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6898
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7450
NheI G'CTAG_C
Cuts at: 1052 3116 4259 1052
Size: 2064 1143 4243
Fragments arranged by size:
4243 2064 1143
NotI GC'GGCC_GC
Cuts at: 4897 4897
Size: 7450
NspI r_CATG'y
Cuts at: 1132 1229 2788 4808 6353 1132
Size: 97 1559 2020 1545 2229
Fragments arranged by size:
2229 2020 1559 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7450
PciI A'CATG_T
Cuts at: 6349 6349
Size: 7450
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2140 3515 1108
Size: 1032 1375 5043
Fragments arranged by size:
5043 1375 1032
PfoI T'CCnGG_A
Cuts at: 2823 2823
Size: 7450
PmeI GTTT'AAAC
Cuts at: 4754 4754
Size: 7450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5519 5551 7032 7064 5519
Size: 32 1481 32 5905
Fragments arranged by size:
5905 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7450
PsiI TTA'TAA
Cuts at: 2379 4721 5020 2379
Size: 2342 299 4809
Fragments arranged by size:
4809 2342 299
PstI C_TGCA'G
Cuts at: 830 2091 4423 4802 830
Size: 1261 2332 379 3478
Fragments arranged by size:
3478 2332 1261 379
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7450
PvuII CAG'CTG
Cuts at: 2921 3502 3718 2921
Size: 581 216 6653
Fragments arranged by size:
6653 581 216
SacI G_AGCT'C
Cuts at: 2018 2018
Size: 7450
SalI G'TCGA_C
Cuts at: 1936 4792 1936
Size: 2856 4594
SapI GCTCTTCn'nnn_
Cuts at: 2006 3438 3955 2006
Size: 1432 517 5501
Fragments arranged by size:
5501 1432 517
SbfI CC_TGCA'GG
Cuts at: 4802 4802
Size: 7450
ScaI AGT'ACT
Cuts at: 5698 7410 5698
Size: 1712 5738
SfcI C'TryA_G
Cuts at: 826 1045 2087 2889 3186 3793 4419 4798 5933
Size: 219 1042 802 297 607 626 379 1135
Cuts at: 5933 6614 6805 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1042 802 681 626 607 379
297 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7450
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7450
SmaI CCC'GGG
Cuts at: 1872 4777 1872
Size: 2905 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2029 2370 2969 3242 4512 4661
Size: 197 926 86 341 599 273 1270 149
Cuts at: 4661 5554 6455 6717 820
Size: 893 901 262 1553
Fragments arranged by size:
1553 1270 926 901 893 599 341 273
262 197 149 86
SnaBI TAC'GTA
Cuts at: 493 5241 493
Size: 4748 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7450
SphI G_CATG'C
Cuts at: 1132 4808 1132
Size: 3676 3774
SspI AAT'ATT
Cuts at: 5 52 2064 3257 5374 5
Size: 47 2012 1193 2117 2081
Fragments arranged by size:
2117 2081 2012 1193 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7450
StyI C'CwwG_G
Cuts at: 513 1065 3537 3990 4188 4276 4889 513
Size: 552 2472 453 198 88 613 3074
Fragments arranged by size:
3074 2472 613 552 453 198 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2870 5477 5494 5647 5832 6277 325
Size: 1255 1290 2607 17 153 185 445 1498
Fragments arranged by size:
2607 1498 1290 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4423 4668 4677
Size: 274 80 33 51 1443 2446 245 9
Cuts at: 4677 5696 7408 96
Size: 1019 1712 138
Fragments arranged by size:
2446 1712 1443 1019 274 245 138 80
51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2235 2267 3371 3403 4167 4199 7410 7442 2235
Size: 32 1104 32 764 32 3211 32 2243
Fragments arranged by size:
3211 2243 1104 764 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7450
XbaI T'CTAG_A
Cuts at: 3955 4786 3955
Size: 831 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4114 4114
Size: 7450
XhoI C'TCGA_G
Cuts at: 1943 4512 1943
Size: 2569 4881
XmaI C'CCGG_G
Cuts at: 1870 4775 1870
Size: 2905 4545
XmnI GAAnn'nnTTC
Cuts at: 2751 3018 4041 5579 2751
Size: 267 1023 1538 4622
Fragments arranged by size:
4622 1538 1023 267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5258 276
Size: 53 83 186 936 3724 2468
Fragments arranged by size:
3724 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI