Restriction map for
pFN21AB0020
(Circular) (Six-base) MAPSORT of: pFN21AB0020.seq Check: 5959 from: 1 to: 10054
pFN21AB0020 10054 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6335 6335
Size: 10054
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6728 7864 278
Size: 53 83 186 936 5192 1136 2468
Fragments arranged by size:
5192 2468 1136 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3009 7397 1937
Size: 1072 4388 4594
Fragments arranged by size:
4594 4388 1072
Acc65I G'GTAC_C
Cuts at: 5612 7375 5612
Size: 1763 8291
AclI AA'CG_TT
Cuts at: 1563 6332 8181 8554 1563
Size: 4769 1849 373 3063
Fragments arranged by size:
4769 3063 1849 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 2442 2753 2958 3201 3357 3801 4737
Size: 729 81 311 205 243 156 444 936
Cuts at: 4737 4806 5013 5186 5268 5282 5925 6126 6548
Size: 69 207 173 82 14 643 201 422
Cuts at: 6548 6828 8117 9501 9769 1632
Size: 280 1289 1384 268 1917
Fragments arranged by size:
1917 1384 1289 936 729 643 444 422
311 280 268 243 207 205 201 173
156 82 81 69 14
AfeI AGC'GCT
Cuts at: 3925 3925
Size: 10054
AflII C'TTAA_G
Cuts at: 820 1017 6490 820
Size: 197 5473 4384
Fragments arranged by size:
5473 4384 197
AflIII A'CryG_T
Cuts at: 1203 3410 3734 8953 9930 1203
Size: 2207 324 5219 977 1327
Fragments arranged by size:
5219 2207 1327 977 324
AgeI A'CCGG_T
Cuts at: 5180 8885 5180
Size: 3705 6349
AhdI GACnn_n'nnGTC
Cuts at: 8783 8783
Size: 10054
AleI CACnn'nnGTG
Cuts at: 1166 4640 5066 6529 1166
Size: 3474 426 1463 4691
Fragments arranged by size:
4691 3474 1463 426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10054
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 10022
AlwNI CAG_nnn'CTG
Cuts at: 2639 3326 3956 4391 4637 4688 4946 5648 6275
Size: 687 630 435 246 51 258 702 627
Cuts at: 6275 6973 7352 7797 9369 2639
Size: 698 379 445 1572 3324
Fragments arranged by size:
3324 1572 702 698 687 630 627 445
435 379 258 246 51
ApaI G_GGCC'C
Cuts at: 6435 6435
Size: 10054
ApaLI G'TGCA_C
Cuts at: 6348 7259 8111 9267 6348
Size: 911 852 1156 7135
Fragments arranged by size:
7135 1156 911 852
ApoI r'AATT_y
Cuts at: 1493 2549 2757 2897 6231 7363 7589 7741 9850
Size: 1056 208 140 3334 1132 226 152 2109
Cuts at: 9850 1493
Size: 1697
Fragments arranged by size:
3334 2109 1697 1132 1056 226 208 152
140
AseI AT'TA_AT
Cuts at: 160 1032 8608 160
Size: 872 7576 1606
Fragments arranged by size:
7576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10054
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2078 3722 4619 4884 7379 1870
Size: 73 108 27 1644 897 265 2495 4545
Fragments arranged by size:
4545 2495 1644 897 265 108 73 27
AvrII C'CTAG_G
Cuts at: 6202 6202
Size: 10054
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4017 4050 4017
Size: 33 10021
BamHI G'GATC_C
Cuts at: 4185 7384 7751 4185
Size: 3199 367 6488
Fragments arranged by size:
6488 3199 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2113 4961 5177 5612
Size: 325 217 627 222 104 2848 216 435
Cuts at: 5612 7375 8830 618
Size: 1763 1455 1842
Fragments arranged by size:
2848 1842 1763 1455 627 435 325 222
217 216 104
BanII G_rGCy'C
Cuts at: 1391 2021 2063 2079 2085 2226 2266 2284 3108
Size: 630 42 16 6 141 40 18 824
Cuts at: 3108 3701 3723 4656 6248 6435 7373 1391
Size: 593 22 933 1592 187 938 4072
Fragments arranged by size:
4072 1592 938 933 824 630 593 187
141 42 40 22 18 16 6
BbeI G_GCGC'C
Cuts at: 2013 2117 2013
Size: 104 9950
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3027 3927 4353 4497 5730 6396 6420
Size: 971 1128 900 426 144 1233 666 24
Cuts at: 6420 928
Size: 4562
Fragments arranged by size:
4562 1233 1128 971 900 666 426 144
24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3683 3717 4967 5001 8245 8279 1903
Size: 34 1746 34 1250 34 3244 34 3678
Fragments arranged by size:
3678 3244 1746 1250 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4018 5779 7946 9156 4018
Size: 1761 2167 1210 4916
Fragments arranged by size:
4916 2167 1761 1210
BclI T'GATC_A
Cuts at: 1548 6964 1548
Size: 5416 4638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 6430 8665
Size: 107 122 71 631 605 439 4319 2235
Cuts at: 8665 136
Size: 1525
Fragments arranged by size:
4319 2235 1525 631 605 439 122 107
71
BglII A'GATC_T
Cuts at: 10049 10049
Size: 10054
BlpI GC'TnA_GC
Cuts at: 2073 2211 2225 3751 4655 7471 2073
Size: 138 14 1526 904 2816 4656
Fragments arranged by size:
4656 2816 1526 904 138 14
Bme1580I G_kGCm'C
Cuts at: 1790 3100 5630 6147 6352 6426 6435 7263 8115
Size: 1310 2530 517 205 74 9 828 852
Cuts at: 8115 9271 1790
Size: 1156 2573
Fragments arranged by size:
2573 2530 1310 1156 852 828 517 205
74 9
BmgBI CAC'GTC
Cuts at: 1318 3265 1318
Size: 1947 8107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5020 5611 7785
Size: 416 53 83 399 24 3603 591 2174
Cuts at: 7785 8743 442
Size: 958 1753
Fragments arranged by size:
3603 2174 1753 958 591 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 3058 3160 3464 5136 6391 2077
Size: 981 102 304 1672 1255 5740
Fragments arranged by size:
5740 1672 1255 981 304 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2691 4368 5117 5652 6113 8714 1239
Size: 977 475 1677 749 535 461 2601 2579
Fragments arranged by size:
2601 2579 1677 977 749 535 475 461
Bpu10I CC'TnA_GC
Cuts at: 5298 5434 5298
Size: 136 9918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2892 5106 5361 5781 5876 6098 6653 8179 9044
Size: 2214 255 420 95 222 555 1526 865
Cuts at: 9044 9342 2892
Size: 298 3604
Fragments arranged by size:
3604 2214 1526 865 555 420 298 255
222 95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2720 4125 4423 4737 4991 6629 8717
Size: 617 1221 1405 298 314 254 1638 2088
Cuts at: 8717 882
Size: 2219
Fragments arranged by size:
2219 2088 1638 1405 1221 617 314 298
254
BsaAI yAC'GTr
Cuts at: 493 1204 3735 6695 6751 7845 493
Size: 711 2531 2960 56 1094 2702
Fragments arranged by size:
2960 2702 2531 1094 711 56
BsaBI GATnn'nnATC
Cuts at: 10048 10048
Size: 10054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2114 3857 6725
Size: 53 83 186 936 477 104 1743 2868
Cuts at: 6725 7861 8243 8878 275
Size: 1136 382 635 1451
Fragments arranged by size:
2868 1743 1451 1136 936 635 477 382
186 104 83 53
BsaWI w'CCGG_w
Cuts at: 3419 3667 5065 5180 8487 8885 9159 9306 9717
Size: 248 1398 115 3307 398 274 147 411
Cuts at: 9717 3419
Size: 3756
Fragments arranged by size:
3756 3307 1398 411 398 274 248 147
115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3719 3749 6848 6878 1178
Size: 30 165 30 2316 30 3099 30 4354
Fragments arranged by size:
4354 3099 2316 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 3164 3293 3630 3633 3636 3695 3731
Size: 1252 726 129 337 3 3 59 36
Cuts at: 3731 3740 4143 4820 5069 5364 5606 5979 6116
Size: 9 403 677 249 295 242 373 137
Cuts at: 6116 6183 6227 6296 6611 6869 7316 1186
Size: 67 44 69 315 258 447 3924
Fragments arranged by size:
3924 1252 726 677 447 403 373 337
315 295 258 249 242 137 129 69
67 59 44 36 9 3 3
BseYI C'CCAG_C
Cuts at: 2084 2657 2767 4358 5166 5438 9257 9753 2084
Size: 573 110 1591 808 272 3819 496 2385
Fragments arranged by size:
3819 2385 1591 808 573 496 272 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3557 3782 5090 5231 5634 6804 1163
Size: 573 1821 225 1308 141 403 1170 4413
Fragments arranged by size:
4413 1821 1308 1170 573 403 225 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 4539 4899 7504 8265 9293
Size: 275 186 105 2433 360 2605 761 1028
Cuts at: 9293 1540
Size: 2301
Fragments arranged by size:
2605 2433 2301 1028 761 360 275 186
105
BsiHKAI G_wGCw'C
Cuts at: 2021 2063 2226 3645 3701 3950 4656 6084 6248
Size: 42 163 1419 56 249 706 1428 164
Cuts at: 6248 6352 7263 8115 8200 9271 2021
Size: 104 911 852 85 1071 2804
Fragments arranged by size:
2804 1428 1419 1071 911 852 706 249
164 163 104 85 56 42
BsmI GAATG_Cn'
Cuts at: 2801 7565 7658 2801
Size: 4764 93 5197
Fragments arranged by size:
5197 4764 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3618 4163 5264 3618
Size: 545 1101 8408
Fragments arranged by size:
8408 1101 545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2063 2079 2085 2226 2266 2284
Size: 399 231 42 16 6 141 40 18
Cuts at: 2284 3100 3108 3645 3701 3723 3950 4656 5630
Size: 816 8 537 56 22 227 706 974
Cuts at: 5630 6084 6147 6248 6352 6426 6435 7263 7373
Size: 454 63 101 104 74 9 828 110
Cuts at: 7373 8115 8200 9271 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 974 828 816 742 706 537
454 399 231 227 141 110 104 101
85 74 63 56 42 40 22 18
16 9 8 6
BspEI T'CCGG_A
Cuts at: 3419 3667 9717 3419
Size: 248 6050 3756
Fragments arranged by size:
6050 3756 248
BspHI T'CATG_A
Cuts at: 7943 9673 7943
Size: 1730 8324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3199 3388 6335 6547 7409 844
Size: 2355 189 2947 212 862 3489
Fragments arranged by size:
3489 2947 2355 862 212 189
BsrBI CCG'CTC
Cuts at: 1394 1823 6401 7941 1394
Size: 429 4578 1540 3507
Fragments arranged by size:
4578 3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4128 8549 8723 65
Size: 1183 2880 4421 174 1396
Fragments arranged by size:
4421 2880 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 2918 3085 5180 8698 8885 1700
Size: 110 258 850 167 2095 3518 187 2869
Fragments arranged by size:
3518 2869 2095 850 258 187 167 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10054
BssSI C'ACGA_G
Cuts at: 3101 8114 9126 3101
Size: 5013 1012 4029
Fragments arranged by size:
5013 4029 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3038 3095 4946 4988 5648 794
Size: 2244 57 1851 42 660 5200
Fragments arranged by size:
5200 2244 1851 660 57 42
BstBI TT'CG_AA
Cuts at: 5847 8858 5847
Size: 3011 7043
BstEII G'GTnAC_C
Cuts at: 1184 2240 1184
Size: 1056 8998
BstXI CCAn_nnnn'nTGG
Cuts at: 3391 5008 5174 6776 3391
Size: 1617 166 1602 6669
Fragments arranged by size:
6669 1617 1602 166
BstYI r'GATC_y
Cuts at: 1972 4185 7384 7751 8136 8153 9605 9691 9703
Size: 2213 3199 367 385 17 1452 86 12
Cuts at: 9703 10049 1972
Size: 346 1977
Fragments arranged by size:
3199 2213 1977 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3366 3714 6810 7165 3366
Size: 348 3096 355 6255
Fragments arranged by size:
6255 3096 355 348
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 4539 4848 6000 513
Size: 552 939 123 2412 309 1152 4567
Fragments arranged by size:
4567 2412 1152 939 552 309 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 487
Size: 683 56 899 8416
Fragments arranged by size:
8416 899 683 56
BtsI GCAGTG_nn'
Cuts at: 2793 5145 6879 7570 8364 8384 2793
Size: 2352 1734 691 794 20 4463
Fragments arranged by size:
4463 2352 1734 794 691 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6855 6890 529
Size: 35 6291 35 3693
Fragments arranged by size:
6291 3693 35 35
DraI TTT'AAA
Cuts at: 2548 4291 7358 2548
Size: 1743 3067 5244
Fragments arranged by size:
5244 3067 1743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4529 9061 809
Size: 507 3213 4532 1802
Fragments arranged by size:
4532 3213 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2251 2779
Size: 54 1425 50 275 18 273 148 528
Cuts at: 2779 3252 4536 7501 8390 10019 8
Size: 473 1284 2965 889 1629 43
Fragments arranged by size:
2965 1629 1425 1284 889 528 473 275
273 148 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2103 4536 7501 1537
Size: 275 291 2433 2965 4090
Fragments arranged by size:
4090 2965 2433 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 2718 2808 3177 3237 3285 3984 4099
Size: 1116 249 90 369 60 48 699 115
Cuts at: 4099 4329 4989 5097 5232 6063 6102 6360 6972
Size: 230 660 108 135 831 39 258 612
Cuts at: 6972 6996 7984 1353
Size: 24 988 3423
Fragments arranged by size:
3423 1116 988 831 699 660 612 369
258 249 230 135 115 108 90 60
48 39 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4925 5131 8641 9015 9161 387
Size: 1064 138 3336 206 3510 374 146 1280
Fragments arranged by size:
3510 3336 1280 1064 374 206 146 138
EcoICRI GAG'CTC
Cuts at: 2019 2061 2224 3699 4654 6246 2019
Size: 42 163 1475 955 1592 5827
Fragments arranged by size:
5827 1592 1475 955 163 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2361 2442 2691 2753 2958 3201
Size: 393 584 145 81 249 62 205 243
Cuts at: 3201 3357 3801 4368 4737 4806 5013 5117 5186
Size: 156 444 567 369 69 207 104 69
Cuts at: 5186 5268 5282 5652 5925 6113 6126 6548 6828
Size: 82 14 370 273 188 13 422 280
Cuts at: 6828 8117 8714 9501 9769 1239
Size: 1289 597 787 268 1524
Fragments arranged by size:
1524 1289 787 597 584 567 444 422
393 370 369 280 273 268 249 243
207 205 188 156 145 104 82 81
69 69 62 14 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2234 4257 2234
Size: 2023 8031
EcoO109I rG'GnC_Cy
Cuts at: 2653 3173 3427 4089 5128 6431 6805 2653
Size: 520 254 662 1039 1303 374 5902
Fragments arranged by size:
5902 1303 1039 662 520 374 254
EcoRI G'AATT_C
Cuts at: 7363 7741 7363
Size: 378 9676
EcoRV GAT'ATC
Cuts at: 4746 4746
Size: 10054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4752 5216 5930 6092 6505 4752
Size: 464 714 162 413 8301
Fragments arranged by size:
8301 714 464 413 162
FspI TGC'GCA
Cuts at: 8560 8560
Size: 10054
HaeII r_GCGC'y
Cuts at: 1678 2013 2117 3927 9201 1678
Size: 335 104 1810 5274 2531
Fragments arranged by size:
5274 2531 1810 335 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3617 3649 3721 3753 3807
Size: 32 135 32 2045 32 72 32 54
Cuts at: 3807 3839 4750 4782 5591 5623 6730 6762 6848
Size: 32 911 32 809 32 1107 32 86
Cuts at: 6848 6880 7310 7342 8695 8727 8769 8801 1373
Size: 32 430 32 1353 32 42 32 2626
Fragments arranged by size:
2626 2045 1353 1107 911 809 430 135
86 72 54 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4800 5031 6682 6723 6867 7398
Size: 988 281 2862 231 1651 41 144 531
Cuts at: 7398 7644 669
Size: 246 3079
Fragments arranged by size:
3079 2862 1651 988 531 281 246 231
144 41
HindIII A'AGCT_T
Cuts at: 748 2970 3557 3584 4044 5359 6074 748
Size: 2222 587 27 460 1315 715 4728
Fragments arranged by size:
4728 2222 1315 715 587 460 27
HpaI GTT'AAC
Cuts at: 4800 5031 7644 4800
Size: 231 2613 7210
Fragments arranged by size:
7210 2613 231
KasI G'GCGC_C
Cuts at: 2009 2113 2009
Size: 104 9950
KpnI G_GTAC'C
Cuts at: 5616 7379 5616
Size: 1763 8291
MfeI C'AATT_G
Cuts at: 6591 7653 6591
Size: 1062 8992
MluI A'CGCG_T
Cuts at: 9930 9930
Size: 10054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2305 2879 6315 6522 9168 9352 1305
Size: 1000 574 3436 207 2646 184 2007
Fragments arranged by size:
3436 2646 2007 1000 574 207 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 2781 10
Size: 54 1425 343 421 528 7283
Fragments arranged by size:
7283 1425 528 421 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4640 5006 5066 6063 6529 6716 8012
Size: 648 3474 366 60 997 466 187 1296
Cuts at: 8012 8371 8530 518
Size: 359 159 2042
Fragments arranged by size:
3474 2042 1296 997 648 466 366 359
187 159 60
MspA1I CmG'CkG
Cuts at: 1606 2088 2129 2188 2390 3119 3335 4688 4937
Size: 482 41 59 202 729 216 1353 249
Cuts at: 4937 5170 6092 6272 6973 7470 8147 9295 9540
Size: 233 922 180 701 497 677 1148 245
Cuts at: 9540 1606
Size: 2120
Fragments arranged by size:
2120 1353 1148 922 729 701 677 497
482 249 245 233 216 202 180 59
41
NaeI GCC'GGC
Cuts at: 2070 2920 3087 2070
Size: 850 167 9037
Fragments arranged by size:
9037 850 167
NarI GG'CG_CC
Cuts at: 2010 2114 2010
Size: 104 9950
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8563
Fragments arranged by size:
8563 939 552
NdeI CA'TA_TG
Cuts at: 387 4674 387
Size: 4287 5767
NgoMIV G'CCGG_C
Cuts at: 2068 2918 3085 2068
Size: 850 167 9037
Fragments arranged by size:
9037 850 167
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10054
NotI GC'GGCC_GC
Cuts at: 7501 7501
Size: 10054
NspI r_CATG'y
Cuts at: 1132 1229 7412 8957 1132
Size: 97 6183 1545 2229
Fragments arranged by size:
6183 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3831 1348
Size: 2483 7571
PciI A'CATG_T
Cuts at: 8953 8953
Size: 10054
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2788 5140 1108
Size: 1680 2352 6022
Fragments arranged by size:
6022 2352 1680
PfoI T'CCnGG_A
Cuts at: 2232 4438 5691 2232
Size: 2206 1253 6595
Fragments arranged by size:
6595 2206 1253
PmeI GTTT'AAAC
Cuts at: 7358 7358
Size: 10054
PmlI CAC'GTG
Cuts at: 3735 6751 3735
Size: 3016 7038
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8123 8155 9636 9668 8123
Size: 32 1481 32 8509
Fragments arranged by size:
8509 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 3173 3427 4089 5128 6805 2653
Size: 520 254 662 1039 1677 5902
Fragments arranged by size:
5902 1677 1039 662 520 254
PshAI GACnn'nnGTC
Cuts at: 1613 3450 1613
Size: 1837 8217
PsiI TTA'TAA
Cuts at: 7624 7624
Size: 10054
PspOMI G'GGCC_C
Cuts at: 6431 6431
Size: 10054
PstI C_TGCA'G
Cuts at: 830 2848 4942 4951 5973 6280 6346 7406 830
Size: 2018 2094 9 1022 307 66 1060 3478
Fragments arranged by size:
3478 2094 2018 1060 1022 307 66 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10054
PvuII CAG'CTG
Cuts at: 2188 3119 3335 4688 4937 5170 6092 6973 2188
Size: 931 216 1353 249 233 922 881 5269
Fragments arranged by size:
5269 1353 931 922 881 249 233 216
SacI G_AGCT'C
Cuts at: 2021 2063 2226 3701 4656 6248 2021
Size: 42 163 1475 955 1592 5827
Fragments arranged by size:
5827 1592 1475 955 163 42
SacII CC_GC'GG
Cuts at: 2130 2130
Size: 10054
SalI G'TCGA_C
Cuts at: 1936 7396 1936
Size: 5460 4594
SapI GCTCTTCn'nnn_
Cuts at: 2469 3285 3984 5097 5232 2469
Size: 816 699 1113 135 7291
Fragments arranged by size:
7291 1113 816 699 135
SbfI CC_TGCA'GG
Cuts at: 6346 7406 6346
Size: 1060 8994
ScaI AGT'ACT
Cuts at: 8302 10014 8302
Size: 1712 8342
SfcI C'TryA_G
Cuts at: 826 1045 2171 2709 2844 4566 4938 4947 5502
Size: 219 1126 538 135 1722 372 9 555
Cuts at: 5502 5865 5969 6276 6342 7402 8537 9218 9409
Size: 363 104 307 66 1060 1135 681 191
Cuts at: 9409 826
Size: 1471
Fragments arranged by size:
1722 1471 1135 1126 1060 681 555 538
372 363 307 219 191 135 104 66
9
SfoI GGC'GCC
Cuts at: 2011 2115 2011
Size: 104 9950
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10054
SgrAI Cr'CCGG_yG
Cuts at: 1700 5180 1700
Size: 3480 6574
SmaI CCC'GGG
Cuts at: 1872 4886 7381 1872
Size: 3014 2495 4545
Fragments arranged by size:
4545 3014 2495
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2907 5121 5376 5796 5855 6077
Size: 197 926 964 2214 255 420 59 222
Cuts at: 6077 6490 6632 8158 9059 9321 820
Size: 413 142 1526 901 262 1553
Fragments arranged by size:
2214 1553 1526 964 926 901 420 413
262 255 222 197 142 59
SnaBI TAC'GTA
Cuts at: 493 6695 7845 493
Size: 6202 1150 2702
Fragments arranged by size:
6202 2702 1150
SpeI A'CTAG_T
Cuts at: 152 2858 7081 152
Size: 2706 4223 3125
Fragments arranged by size:
4223 3125 2706
SphI G_CATG'C
Cuts at: 1132 7412 1132
Size: 6280 3774
SspI AAT'ATT
Cuts at: 5 52 7978 5
Size: 47 7926 2081
Fragments arranged by size:
7926 2081 47
StuI AGG'CCT
Cuts at: 1517 5665 6815 1517
Size: 4148 1150 4756
Fragments arranged by size:
4756 4148 1150
StyI C'CwwG_G
Cuts at: 513 1065 2004 2662 3384 4647 6202 7493 513
Size: 552 939 658 722 1263 1555 1291 3074
Fragments arranged by size:
3074 1555 1291 1263 939 722 658 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3090 4949 8081 8098 8251 8436 8881
Size: 1255 1510 1859 3132 17 153 185 445
Cuts at: 8881 325
Size: 1498
Fragments arranged by size:
3132 1859 1510 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 8300 10012 96
Size: 274 80 33 51 1443 6323 1712 138
Fragments arranged by size:
6323 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3637 3669 3719 3751 10014 10046 3637
Size: 32 50 32 6263 32 3645
Fragments arranged by size:
6263 3645 50 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2588 3914 4895 1316
Size: 1272 1326 981 6475
Fragments arranged by size:
6475 1326 1272 981
XbaI T'CTAG_A
Cuts at: 5731 7390 5731
Size: 1659 8395
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10054
XmaI C'CCGG_G
Cuts at: 1870 4884 7379 1870
Size: 3014 2495 4545
Fragments arranged by size:
4545 3014 2495
XmnI GAAnn'nnTTC
Cuts at: 2448 8183 2448
Size: 5735 4319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6726 7862 276
Size: 53 83 186 936 5192 1136 2468
Fragments arranged by size:
5192 2468 1136 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbvCI BfrBI BsiWI BstZ17I DraIII FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI
SfiI SrfI SwaI XcmI