Restriction map for
pFN21AB0015
(Circular) (Six-base) MAPSORT of: pFN21AB0015.seq Check: 3988 from: 1 to: 9478
pFN21AB0015 9478 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5164 5617 5164
Size: 453 9025
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3720 4119 7288 278
Size: 53 83 186 936 2184 399 3169 2468
Fragments arranged by size:
3169 2468 2184 936 399 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4232 6821 1937
Size: 2295 2589 4594
Fragments arranged by size:
4594 2589 2295
Acc65I G'GTAC_C
Cuts at: 6799 6799
Size: 9478
AclI AA'CG_TT
Cuts at: 1563 7605 7978 1563
Size: 6042 373 3063
Fragments arranged by size:
6042 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 4323 4649 4696 4871 5636 5891 5903
Size: 638 2053 326 47 175 765 255 12
Cuts at: 5903 6143 7541 8925 9193 1632
Size: 240 1398 1384 268 1917
Fragments arranged by size:
2053 1917 1398 1384 765 638 326 268
255 240 175 47 12
AflII C'TTAA_G
Cuts at: 820 1017 3356 820
Size: 197 2339 6942
Fragments arranged by size:
6942 2339 197
AflIII A'CryG_T
Cuts at: 1203 3381 4235 4440 8377 9354 1203
Size: 2178 854 205 3937 977 1327
Fragments arranged by size:
3937 2178 1327 977 854 205
AgeI A'CCGG_T
Cuts at: 8309 8309
Size: 9478
AhdI GACnn_n'nnGTC
Cuts at: 2259 4024 5470 8207 2259
Size: 1765 1446 2737 3530
Fragments arranged by size:
3530 2737 1765 1446
AleI CACnn'nnGTG
Cuts at: 1166 2072 2225 6417 1166
Size: 906 153 4192 4227
Fragments arranged by size:
4227 4192 906 153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2466 3612 4010 5052 1184
Size: 1282 1146 398 1042 5610
Fragments arranged by size:
5610 1282 1146 1042 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 9446
AlwNI CAG_nnn'CTG
Cuts at: 2680 3568 5542 7221 8793 2680
Size: 888 1974 1679 1572 3365
Fragments arranged by size:
3365 1974 1679 1572 888
ApaI G_GGCC'C
Cuts at: 4884 5266 6469 6519 4884
Size: 382 1203 50 7843
Fragments arranged by size:
7843 1203 382 50
ApaLI G'TGCA_C
Cuts at: 2642 3424 7535 8691 2642
Size: 782 4111 1156 3429
Fragments arranged by size:
4111 3429 1156 782
ApoI r'AATT_y
Cuts at: 1493 2039 2060 2364 4367 4954 5249 6731 6787
Size: 546 21 304 2003 587 295 1482 56
Cuts at: 6787 7013 7165 9274 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 2003 1697 1482 587 546 304 295
226 152 56 21
AseI AT'TA_AT
Cuts at: 160 1032 8032 160
Size: 872 7000 1606
Fragments arranged by size:
7000 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9478
AvaI C'yCGr_G
Cuts at: 1870 1943 2700 4308 6518 6564 6726 6803 1870
Size: 73 757 1608 2210 46 162 77 4545
Fragments arranged by size:
4545 2210 1608 757 162 77 73 46
AvrII C'CTAG_G
Cuts at: 3064 3064
Size: 9478
BamHI G'GATC_C
Cuts at: 3132 3860 6808 7175 3132
Size: 728 2948 367 5435
Fragments arranged by size:
5435 2948 728 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3785 5176 5957 6639 6799
Size: 325 217 627 1998 1391 781 682 160
Cuts at: 6799 8254 618
Size: 1455 1842
Fragments arranged by size:
1998 1842 1455 1391 781 682 627 325
217 160
BanII G_rGCy'C
Cuts at: 1391 3234 4884 5266 5658 5892 6149 6469 6519
Size: 1843 1650 382 392 234 257 320 50
Cuts at: 6519 6797 1391
Size: 278 4072
Fragments arranged by size:
4072 1843 1650 392 382 320 278 257
234 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 2544 3712 5332 5867 928
Size: 971 359 286 1168 1620 535 4539
Fragments arranged by size:
4539 1620 1168 971 535 359 286
BbvCI CC'TCA_GC
Cuts at: 3624 5773 3624
Size: 2149 7329
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7669 7703 1903
Size: 34 5732 34 3678
Fragments arranged by size:
5732 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5251 7370 8580 5251
Size: 2119 1210 6149
Fragments arranged by size:
6149 2119 1210
BclI T'GATC_A
Cuts at: 1548 2125 1548
Size: 577 8901
BfrBI ATG'CAT
Cuts at: 3098 5057 5753 3098
Size: 1959 696 6823
Fragments arranged by size:
6823 1959 696
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3205 5777 6648
Size: 107 122 71 631 605 1533 2572 871
Cuts at: 6648 8089 136
Size: 1441 1525
Fragments arranged by size:
2572 1533 1525 1441 871 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2331 3037 3696 6151 9473 2331
Size: 706 659 2455 3322 2336
Fragments arranged by size:
3322 2455 2336 706 659
BlpI GC'TnA_GC
Cuts at: 5657 5990 6895 5657
Size: 333 905 8240
Fragments arranged by size:
8240 905 333
Bme1580I G_kGCm'C
Cuts at: 1790 2646 3428 3832 4884 5266 5744 5960 6469
Size: 856 782 404 1052 382 478 216 509
Cuts at: 6469 6519 6644 7539 8695 1790
Size: 50 125 895 1156 2573
Fragments arranged by size:
2573 1156 1052 895 856 782 509 478
404 382 216 125 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9478
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2518 6318 6472
Size: 416 53 83 399 24 1101 3800 154
Cuts at: 6472 7209 8167 442
Size: 737 958 1753
Fragments arranged by size:
3800 1753 1101 958 737 416 399 154
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 9478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3143 4851 5150 5576 5742 6158 8138 1239
Size: 1904 1708 299 426 166 416 1980 2579
Fragments arranged by size:
2579 1980 1904 1708 426 416 299 166
Bpu10I CC'TnA_GC
Cuts at: 3624 5773 5781 3624
Size: 2149 8 7321
Fragments arranged by size:
7321 2149 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5993 6338 7603 8468 8766 5993
Size: 345 1265 865 298 6705
Fragments arranged by size:
6705 1265 865 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2579 8141 882
Size: 617 1080 5562 2219
Fragments arranged by size:
5562 2219 1080 617
BsaAI yAC'GTr
Cuts at: 493 1204 3382 3877 4225 5146 7269 493
Size: 711 2178 495 348 921 2123 2702
Fragments arranged by size:
2702 2178 2123 921 711 495 348
BsaBI GATnn'nnATC
Cuts at: 9472 9472
Size: 9478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3717 4116 5763 7285
Size: 53 83 186 936 2184 399 1647 1522
Cuts at: 7285 7667 8302 275
Size: 382 635 1451
Fragments arranged by size:
2184 1647 1522 1451 936 635 399 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3630 5373 6690 7911 8309 8583 8730 9141 3630
Size: 1743 1317 1221 398 274 147 411 3967
Fragments arranged by size:
3967 1743 1317 1221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3013 3043 3938 3968 5549
Size: 30 165 30 1610 30 895 30 1581
Cuts at: 5549 5579 1178
Size: 30 5077
Fragments arranged by size:
5077 1610 1581 895 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2346 2423 2782 3104 3423 3962 3967 4217
Size: 1160 77 359 322 319 539 5 250
Cuts at: 4217 4302 4326 5078 5434 6524 6721 6782 1186
Size: 85 24 752 356 1090 197 61 3882
Fragments arranged by size:
3882 1160 1090 752 539 359 356 322
319 250 197 85 77 61 24 5
BseYI C'CCAG_C
Cuts at: 2625 4646 4883 5188 5396 5485 5961 8681 9177
Size: 2021 237 305 208 89 476 2720 496
Cuts at: 9177 2625
Size: 2926
Fragments arranged by size:
2926 2720 2021 496 476 305 237 208
89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3767 4247 5096 5555 6081 1163
Size: 573 2031 480 849 459 526 4560
Fragments arranged by size:
4560 2031 849 573 526 480 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6928 7689 8717 1540
Size: 275 186 4927 761 1028 2301
Fragments arranged by size:
4927 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2646 3127 3428 4472 5658 5892 6318 7539
Size: 541 481 301 1044 1186 234 426 1221
Cuts at: 7539 7624 8695 2105
Size: 85 1071 2888
Fragments arranged by size:
2888 1221 1186 1071 1044 541 481 426
301 234 85
BsmI GAATG_Cn'
Cuts at: 3100 4887 5390 5755 6989 7082 3100
Size: 1787 503 365 1234 93 5496
Fragments arranged by size:
5496 1787 1234 503 365 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2303 2864 4162 4308 2303
Size: 561 1298 146 7473
Fragments arranged by size:
7473 1298 561 146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2646 3127 3234 3428 3832 4472
Size: 399 315 541 481 107 194 404 640
Cuts at: 4472 4884 5266 5658 5744 5892 5960 6149 6318
Size: 412 382 392 86 148 68 189 169
Cuts at: 6318 6469 6519 6644 6797 7539 7624 8695 1391
Size: 151 50 125 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 541 481 412 404
399 392 382 315 194 189 169 153
151 148 125 107 86 85 68 50
BspEI T'CCGG_A
Cuts at: 5373 6690 9141 5373
Size: 1317 2451 5710
Fragments arranged by size:
5710 2451 1317
BspHI T'CATG_A
Cuts at: 7367 9097 7367
Size: 1730 7748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 5118 5164 5617 6833 844
Size: 2074 2200 46 453 1216 3489
Fragments arranged by size:
3489 2200 2074 1216 453 46
BsrBI CCG'CTC
Cuts at: 1394 1823 2752 7365 1394
Size: 429 929 4613 3507
Fragments arranged by size:
4613 3507 929 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2504 3342 3504 6121 6718 7973 8147
Size: 1183 1256 838 162 2617 597 1255 174
Cuts at: 8147 65
Size: 1396
Fragments arranged by size:
2617 1396 1256 1255 1183 838 597 174
162
BsrFI r'CCGG_y
Cuts at: 1700 1810 2659 8122 8309 1700
Size: 110 849 5463 187 2869
Fragments arranged by size:
5463 2869 849 187 110
BsrGI T'GTAC_A
Cuts at: 96 6183 96
Size: 6087 3391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9478
BssSI C'ACGA_G
Cuts at: 7538 8550 7538
Size: 1012 8466
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3520 3525 5209 794
Size: 2726 5 1684 5063
Fragments arranged by size:
5063 2726 1684 5
BstBI TT'CG_AA
Cuts at: 8282 8282
Size: 9478
BstEII G'GTnAC_C
Cuts at: 1184 3185 1184
Size: 2001 7477
BstXI CCAn_nnnn'nTGG
Cuts at: 3820 4759 6245 3820
Size: 939 1486 7053
Fragments arranged by size:
7053 1486 939
BstYI r'GATC_y
Cuts at: 1972 2331 3037 3132 3565 3672 3696 3860 5152
Size: 359 706 95 433 107 24 164 1292
Cuts at: 5152 6151 6657 6808 7175 7560 7577 9029 9115
Size: 999 506 151 367 385 17 1452 86
Cuts at: 9115 9127 9473 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1292 999 706 506 433 385
367 359 346 164 151 107 95 86
24 17 12
BstZ17I GTA'TAC
Cuts at: 4233 4233
Size: 9478
Bsu36I CC'TnA_GG
Cuts at: 3087 4986 3087
Size: 1899 7579
BtgI C'CryG_G
Cuts at: 513 1065 2223 5574 6762 513
Size: 552 1158 3351 1188 3229
Fragments arranged by size:
3351 3229 1188 1158 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8739
Fragments arranged by size:
8739 683 56
BtsI GCAGTG_nn'
Cuts at: 2009 2511 2587 3275 4288 4467 5973 6062 6994
Size: 502 76 688 1013 179 1506 89 932
Cuts at: 6994 7788 7808 2009
Size: 794 20 3679
Fragments arranged by size:
3679 1506 1013 932 794 688 502 179
89 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9443
DraI TTT'AAA
Cuts at: 6782 6782
Size: 9478
DraIII CAC_nnn'GTG
Cuts at: 5853 5853
Size: 9478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8485 809
Size: 507 7169 1802
Fragments arranged by size:
7169 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2920 3456 3651
Size: 54 1425 50 275 18 1090 536 195
Cuts at: 3651 4072 4479 5715 6925 7814 9443 8
Size: 421 407 1236 1210 889 1629 43
Fragments arranged by size:
1629 1425 1236 1210 1090 889 536 421
407 275 195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6925 1537
Size: 275 5113 4090
Fragments arranged by size:
5113 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 3360 4130 6137 6422 7408 1353
Size: 740 1267 770 2007 285 986 3423
Fragments arranged by size:
3423 2007 1267 986 770 740 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8065 8439 8585 387
Size: 1064 138 6476 374 146 1280
Fragments arranged by size:
6476 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5656 5890 5656
Size: 234 9244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 3143 4323 4649 4696 4851 4871
Size: 393 638 873 1180 326 47 155 20
Cuts at: 4871 5150 5576 5636 5742 5891 5903 6143 6158
Size: 279 426 60 106 149 12 240 15
Cuts at: 6158 7541 8138 8925 9193 1239
Size: 1383 597 787 268 1524
Fragments arranged by size:
1524 1383 1180 873 787 638 597 426
393 326 279 268 240 155 149 106
60 47 20 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 5729 5729
Size: 9478
EcoO109I rG'GnC_Cy
Cuts at: 2279 3068 3835 4880 5262 5263 5769 6466 6515
Size: 789 767 1045 382 1 506 697 49
Cuts at: 6515 2279
Size: 5242
Fragments arranged by size:
5242 1045 789 767 697 506 382 49
1
EcoRI G'AATT_C
Cuts at: 4954 5249 6787 7165 4954
Size: 295 1538 378 7267
Fragments arranged by size:
7267 1538 378 295
EcoRV GAT'ATC
Cuts at: 2401 2401
Size: 9478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4351 6115 6148 4351
Size: 1764 33 7681
Fragments arranged by size:
7681 1764 33
FspI TGC'GCA
Cuts at: 7984 7984
Size: 9478
HaeII r_GCGC'y
Cuts at: 1678 8625 1678
Size: 6947 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2245 2277 3083 3115 3359
Size: 32 135 32 673 32 806 32 244
Cuts at: 3359 3391 3773 3805 4010 4042 4164 4196 4281
Size: 32 382 32 205 32 122 32 85
Cuts at: 4281 4313 4661 4693 5225 5257 5456 5488 6136
Size: 32 348 32 532 32 199 32 648
Cuts at: 6136 6143 6168 6175 6685 6717 8119 8151 8193
Size: 7 25 7 510 32 1402 32 42
Cuts at: 8193 8225 1373
Size: 32 2626
Fragments arranged by size:
2626 1402 806 673 648 532 510 382
348 244 205 199 135 122 85 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 6822 7068 669
Size: 988 281 4884 246 3079
Fragments arranged by size:
4884 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9478
HpaI GTT'AAC
Cuts at: 7068 7068
Size: 9478
KpnI G_GTAC'C
Cuts at: 6803 6803
Size: 9478
MfeI C'AATT_G
Cuts at: 7077 7077
Size: 9478
MluI A'CGCG_T
Cuts at: 9354 9354
Size: 9478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 4195 4578 8592 8776 1305
Size: 942 1948 383 4014 184 2007
Fragments arranged by size:
4014 2007 1948 942 383 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2922 3653 4074 4481 10
Size: 54 1425 343 1090 731 421 407 5007
Fragments arranged by size:
5007 1425 1090 731 421 407 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2225 3647 3653 3818 3992 5522
Size: 648 906 153 1422 6 165 174 1530
Cuts at: 5522 6417 6666 7436 7795 7954 518
Size: 895 249 770 359 159 2042
Fragments arranged by size:
2042 1530 1422 906 895 770 648 359
249 174 165 159 153 6
MspA1I CmG'CkG
Cuts at: 1606 2680 3163 3592 4849 5554 5777 5965 6368
Size: 1074 483 429 1257 705 223 188 403
Cuts at: 6368 6627 6894 7571 8719 8964 1606
Size: 259 267 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 1074 705 677 483 429
403 267 259 245 223 188
NcoI C'CATG_G
Cuts at: 513 1065 2223 5574 513
Size: 552 1158 3351 4417
Fragments arranged by size:
4417 3351 1158 552
NdeI CA'TA_TG
Cuts at: 387 2071 4798 6663 387
Size: 1684 2727 1865 3202
Fragments arranged by size:
3202 2727 1865 1684
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9478
NotI GC'GGCC_GC
Cuts at: 6925 6925
Size: 9478
NsiI A_TGCA'T
Cuts at: 3100 5059 5755 3100
Size: 1959 696 6823
Fragments arranged by size:
6823 1959 696
NspI r_CATG'y
Cuts at: 1132 1229 2991 3253 3523 4239 4425 4444 5312
Size: 97 1762 262 270 716 186 19 868
Cuts at: 5312 6836 8381 1132
Size: 1524 1545 2229
Fragments arranged by size:
2229 1762 1545 1524 868 716 270 262
186 97 19
PasI CC'CwG_GG
Cuts at: 1348 3832 1348
Size: 2484 6994
PciI A'CATG_T
Cuts at: 4235 4440 8377 4235
Size: 205 3937 5336
Fragments arranged by size:
5336 3937 205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2424 3182 1108
Size: 1316 758 7404
Fragments arranged by size:
7404 1316 758
PfoI T'CCnGG_A
Cuts at: 3210 3711 5820 6135 3210
Size: 501 2109 315 6553
Fragments arranged by size:
6553 2109 501 315
PmeI GTTT'AAAC
Cuts at: 6782 6782
Size: 9478
PmlI CAC'GTG
Cuts at: 3382 4225 3382
Size: 843 8635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 7547 7579 9060 9092 3938
Size: 32 3577 32 1481 32 4324
Fragments arranged by size:
4324 3577 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3068 3068
Size: 9478
PshAI GACnn'nnGTC
Cuts at: 1613 2178 1613
Size: 565 8913
PsiI TTA'TAA
Cuts at: 7048 7048
Size: 9478
PspOMI G'GGCC_C
Cuts at: 4880 5262 6465 6515 4880
Size: 382 1203 50 7843
Fragments arranged by size:
7843 1203 382 50
PspXI vC'TCGA_Gb
Cuts at: 4308 4308
Size: 9478
PstI C_TGCA'G
Cuts at: 830 3168 3402 3530 3591 3601 5608 5628 6159
Size: 2338 234 128 61 10 2007 20 531
Cuts at: 6159 6830 830
Size: 671 3478
Fragments arranged by size:
3478 2338 2007 671 531 234 128 61
20 10
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9478
PvuII CAG'CTG
Cuts at: 2680 3163 4849 5554 5965 6627 2680
Size: 483 1686 705 411 662 5531
Fragments arranged by size:
5531 1686 705 662 483 411
SacI G_AGCT'C
Cuts at: 5658 5892 5658
Size: 234 9244
SalI G'TCGA_C
Cuts at: 1936 6820 1936
Size: 4884 4594
SapI GCTCTTCn'nnn_
Cuts at: 2093 3360 6137 2093
Size: 1267 2777 5434
Fragments arranged by size:
5434 2777 1267
SbfI CC_TGCA'GG
Cuts at: 3402 6830 3402
Size: 3428 6050
ScaI AGT'ACT
Cuts at: 7726 9438 7726
Size: 1712 7766
SfcI C'TryA_G
Cuts at: 826 1045 2109 3164 3398 3526 3587 3597 5604
Size: 219 1064 1055 234 128 61 10 2007
Cuts at: 5604 5624 6155 6826 7961 8642 8833 826
Size: 20 531 671 1135 681 191 1471
Fragments arranged by size:
2007 1471 1135 1064 1055 681 671 531
234 219 191 128 61 20 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9478
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9478
SmaI CCC'GGG
Cuts at: 1872 2702 6566 6805 1872
Size: 830 3864 239 4545
Fragments arranged by size:
4545 3864 830 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3356 4308 6008 6353 7582 8483
Size: 197 926 1413 952 1700 345 1229 901
Cuts at: 8483 8745 820
Size: 262 1553
Fragments arranged by size:
1700 1553 1413 1229 952 926 901 345
262 197
SnaBI TAC'GTA
Cuts at: 493 7269 493
Size: 6776 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9478
SphI G_CATG'C
Cuts at: 1132 3253 3523 5312 6836 1132
Size: 2121 270 1789 1524 3774
Fragments arranged by size:
3774 2121 1789 1524 270
SspI AAT'ATT
Cuts at: 5 52 7402 5
Size: 47 7350 2081
Fragments arranged by size:
7350 2081 47
StuI AGG'CCT
Cuts at: 1517 3404 3492 4781 6678 1517
Size: 1887 88 1289 1897 4317
Fragments arranged by size:
4317 1897 1887 1289 88
StyI C'CwwG_G
Cuts at: 513 1065 2223 2454 2952 2997 3064 3147 3846
Size: 552 1158 231 498 45 67 83 699
Cuts at: 3846 4069 5574 5982 6238 6618 6645 6917 513
Size: 223 1505 408 256 380 27 272 3074
Fragments arranged by size:
3074 1505 1158 699 552 498 408 380
272 256 231 223 83 67 45 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2577 5975 6251 7505 7522 7675 7860
Size: 1255 997 3398 276 1254 17 153 185
Cuts at: 7860 8305 325
Size: 445 1498
Fragments arranged by size:
3398 1498 1255 1254 997 445 276 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2086 3775 4332
Size: 274 80 33 51 1443 109 1689 557
Cuts at: 4332 4437 5878 6183 6474 7724 9436 96
Size: 105 1441 305 291 1250 1712 138
Fragments arranged by size:
1712 1689 1443 1441 1250 557 305 291
274 138 109 105 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2894 2926 3117 3149 4087 4119 9438 9470 2894
Size: 32 191 32 938 32 5319 32 2902
Fragments arranged by size:
5319 2902 938 191 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9478
XbaI T'CTAG_A
Cuts at: 6814 6814
Size: 9478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2930 2930
Size: 9478
XhoI C'TCGA_G
Cuts at: 1943 4308 1943
Size: 2365 7113
XmaI C'CCGG_G
Cuts at: 1870 2700 6564 6803 1870
Size: 830 3864 239 4545
Fragments arranged by size:
4545 3864 830 239
XmnI GAAnn'nnTTC
Cuts at: 3328 4253 4637 4662 6131 7607 3328
Size: 925 384 25 1469 1476 5199
Fragments arranged by size:
5199 1476 1469 925 384 25
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3718 4117 7286 276
Size: 53 83 186 936 2184 399 3169 2468
Fragments arranged by size:
3169 2468 2184 936 399 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BsiWI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI