Restriction map for
pFN21AB0013
(Circular) (Six-base) MAPSORT of: pFN21AB0013.seq Check: 2832 from: 1 to: 11176
pFN21AB0013 11176 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2067 4464 5127 5195 6398 8250 8311 2067
Size: 2397 663 68 1203 1852 61 4932
Fragments arranged by size:
4932 2397 1852 1203 663 68 61
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2145 6084 7614 8009
Size: 53 83 186 936 609 3939 1530 395
Cuts at: 8009 8986 278
Size: 977 2468
Fragments arranged by size:
3939 2468 1530 977 936 609 395 186
83 53
AccI GT'mk_AC
Cuts at: 1937 6234 7110 8519 1937
Size: 4297 876 1409 4594
Fragments arranged by size:
4594 4297 1409 876
Acc65I G'GTAC_C
Cuts at: 5901 8497 5901
Size: 2596 8580
AclI AA'CG_TT
Cuts at: 1563 9303 9676 1563
Size: 7740 373 3063
Fragments arranged by size:
7740 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 3099 3155 3350 3620 4844 5119 5718
Size: 881 586 56 195 270 1224 275 599
Cuts at: 5718 7337 9239 10623 10891 1632
Size: 1619 1902 1384 268 1917
Fragments arranged by size:
1917 1902 1619 1384 1224 881 599 586
275 270 268 195 56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10979
AflIII A'CryG_T
Cuts at: 1203 2532 2644 4683 6903 7168 10075 11052 1203
Size: 1329 112 2039 2220 265 2907 977 1327
Fragments arranged by size:
2907 2220 2039 1329 1327 977 265 112
AgeI A'CCGG_T
Cuts at: 10007 10007
Size: 11176
AhdI GACnn_n'nnGTC
Cuts at: 3316 4393 9905 3316
Size: 1077 5512 4587
Fragments arranged by size:
5512 4587 1077
AleI CACnn'nnGTG
Cuts at: 1166 7310 7631 1166
Size: 6144 321 4711
Fragments arranged by size:
6144 4711 321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3225 3556 4439 6894 7434 1184
Size: 2041 331 883 2455 540 4926
Fragments arranged by size:
4926 2455 2041 883 540 331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6491 6523 6491
Size: 32 11144
AlwNI CAG_nnn'CTG
Cuts at: 2626 3328 3823 4621 5104 5210 5998 6787 7252
Size: 702 495 798 483 106 788 789 465
Cuts at: 7252 7327 8919 10491 2626
Size: 75 1592 1572 3311
Fragments arranged by size:
3311 1592 1572 798 789 788 702 495
483 465 106 75
ApaI G_GGCC'C
Cuts at: 8067 8067
Size: 11176
ApaLI G'TGCA_C
Cuts at: 2258 7025 9233 10389 2258
Size: 4767 2208 1156 3045
Fragments arranged by size:
4767 3045 2208 1156
ApoI r'AATT_y
Cuts at: 1493 2897 3059 8485 8711 8863 10972 1493
Size: 1404 162 5426 226 152 2109 1697
Fragments arranged by size:
5426 2109 1697 1404 226 162 152
AscI GG'CGCG_CC
Cuts at: 8094 8094
Size: 11176
AseI AT'TA_AT
Cuts at: 160 1032 9730 160
Size: 872 8698 1606
Fragments arranged by size:
8698 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11176
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2468 2716 3299 5810 5819 6220
Size: 73 86 439 248 583 2511 9 401
Cuts at: 6220 7233 7340 7508 7818 8104 8265 8345 8458
Size: 1013 107 168 310 286 161 80 113
Cuts at: 8458 8501 1870
Size: 43 4545
Fragments arranged by size:
4545 2511 1013 583 439 401 310 286
248 168 161 113 107 86 80 73
43 9
BamHI G'GATC_C
Cuts at: 7911 8428 8506 8873 7911
Size: 517 78 367 10214
Fragments arranged by size:
10214 517 367 78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2403 3872 4160 5070
Size: 325 217 627 393 223 1469 288 910
Cuts at: 5070 5115 5289 5901 7138 8254 8261 8289 8369
Size: 45 174 612 1237 1116 7 28 80
Cuts at: 8369 8497 9952 618
Size: 128 1455 1842
Fragments arranged by size:
1842 1469 1455 1237 1116 910 627 612
393 325 288 223 217 174 128 80
45 28 7
BanII G_rGCy'C
Cuts at: 1391 4026 4545 5732 6227 6537 7242 7347 7811
Size: 2635 519 1187 495 310 705 105 464
Cuts at: 7811 7979 8067 8111 8382 8495 1391
Size: 168 88 44 271 113 4072
Fragments arranged by size:
4072 2635 1187 705 519 495 464 310
271 168 113 105 88 44
BbeI G_GCGC'C
Cuts at: 2407 8293 8373 2407
Size: 5886 80 5210
Fragments arranged by size:
5886 5210 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2641 3585 4810 4814 5384 5586 5717
Size: 971 742 944 1225 4 570 202 131
Cuts at: 5717 6596 8180 928
Size: 879 1584 3924
Fragments arranged by size:
3924 1584 1225 971 944 879 742 570
202 131 4
BbvCI CC'TCA_GC
Cuts at: 2780 3416 5211 6005 2780
Size: 636 1795 794 7951
Fragments arranged by size:
7951 1795 794 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2010 2044 4121 4155 9367 9401 1903
Size: 34 73 34 2077 34 5212 34 3678
Fragments arranged by size:
5212 3678 2077 73 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9068 10278 9068
Size: 1210 9966
BclI T'GATC_A
Cuts at: 1548 4242 7644 1548
Size: 2694 3402 5080
Fragments arranged by size:
5080 3402 2694
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2666 2790 4127
Size: 107 122 71 631 605 994 124 1337
Cuts at: 4127 4136 5880 6029 6300 7098 8270 9787 136
Size: 9 1744 149 271 798 1172 1517 1525
Fragments arranged by size:
1744 1525 1517 1337 1172 994 798 631
605 271 149 124 122 107 71 9
BglII A'GATC_T
Cuts at: 2526 11171 2526
Size: 8645 2531
BlpI GC'TnA_GC
Cuts at: 5256 5987 6114 8593 5256
Size: 731 127 2479 7839
Fragments arranged by size:
7839 2479 731 127
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2290 3875 4165 5118 5292 5527 6336
Size: 472 28 1585 290 953 174 235 809
Cuts at: 6336 6903 7029 7143 8067 8257 8266 9237 10393
Size: 567 126 114 924 190 9 971 1156
Cuts at: 10393 1790
Size: 2573
Fragments arranged by size:
2573 1585 1156 971 953 924 809 567
472 290 235 190 174 126 114 28
9
BmgBI CAC'GTC
Cuts at: 1318 2647 2665 3523 5479 1318
Size: 1329 18 858 1956 7015
Fragments arranged by size:
7015 1956 1329 858 18
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4486 4538 5715
Size: 416 53 83 399 24 3069 52 1177
Cuts at: 5715 7778 8276 8907 9865 442
Size: 2063 498 631 958 1753
Fragments arranged by size:
3069 2063 1753 1177 958 631 498 416
399 83 53 52 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11176
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 3553 4771 5362 6867 2746
Size: 807 1218 591 1505 7055
Fragments arranged by size:
7055 1505 1218 807 591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 2861 2866 3689 4280 4298 4661 4787
Size: 1418 204 5 823 591 18 363 126
Cuts at: 4787 4937 5012 5021 5259 5492 6317 6335 7172
Size: 150 75 9 238 233 825 18 837
Cuts at: 7172 7301 7457 7490 7718 7862 7874 8051 8396
Size: 129 156 33 228 144 12 177 345
Cuts at: 8396 9836 1239
Size: 1440 2579
Fragments arranged by size:
2579 1440 1418 837 825 823 591 363
345 238 233 228 204 177 156 150
144 129 126 75 33 18 18 12
9 5
Bpu10I CC'TnA_GC
Cuts at: 2684 2780 3416 5211 6005 2684
Size: 96 636 1795 794 7855
Fragments arranged by size:
7855 1795 794 636 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 3053 3140 6521 7601 9301 10166 10464 2486
Size: 567 87 3381 1080 1700 865 298 3198
Fragments arranged by size:
3381 3198 1700 1080 865 567 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 5698 5807 9839 882
Size: 617 3138 1061 109 4032 2219
Fragments arranged by size:
4032 3138 2219 1061 617 109
BsaAI yAC'GTr
Cuts at: 493 1204 2365 2535 2602 3702 4015 6337 6682
Size: 711 1161 170 67 1100 313 2322 345
Cuts at: 6682 6904 7171 7629 8967 493
Size: 222 267 458 1338 2702
Fragments arranged by size:
2702 2322 1338 1161 1100 711 458 345
313 267 222 170 67
BsaBI GATnn'nnATC
Cuts at: 7643 11170 7643
Size: 3527 7649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2154 2241 2404
Size: 53 83 186 936 609 12 87 163
Cuts at: 2404 2805 4111 5910 6081 6593 7611 8006 8290
Size: 401 1306 1799 171 512 1018 395 284
Cuts at: 8290 8370 8983 9365 10000 275
Size: 80 613 382 635 1451
Fragments arranged by size:
1799 1451 1306 1018 936 635 613 609
512 401 395 382 284 186 171 163
87 83 80 53 12
BsaWI w'CCGG_w
Cuts at: 4656 4905 4989 6383 9609 10007 10281 10428 10839
Size: 249 84 1394 3226 398 274 147 411
Cuts at: 10839 4656
Size: 4993
Fragments arranged by size:
4993 3226 1394 411 398 274 249 147
84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 2931 2961 6179
Size: 30 165 30 1461 30 37 30 3218
Cuts at: 6179 6209 7448 7478 7548 7578 1178
Size: 30 1239 30 70 30 4776
Fragments arranged by size:
4776 3218 1461 1239 165 70 37 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2952 3497 3803 4206 5228 5335 5531 6065
Size: 1766 545 306 403 1022 107 196 534
Cuts at: 6065 6083 6323 7697 7847 7850 7871 7874 7943
Size: 18 240 1374 150 3 21 3 69
Cuts at: 7943 8000 8048 1186
Size: 57 48 4314
Fragments arranged by size:
4314 1766 1374 1022 545 534 403 306
240 196 150 107 69 57 48 21
18 3 3
BseYI C'CCAG_C
Cuts at: 2073 2626 4056 4734 5429 8250 10379 10875 2073
Size: 553 1430 678 695 2821 2129 496 2374
Fragments arranged by size:
2821 2374 2129 1430 695 678 553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 4409 4436 4442 4844 5333 8384
Size: 573 309 2364 27 6 402 489 3051
Cuts at: 8384 1163
Size: 3955
Fragments arranged by size:
3955 3051 2364 573 489 402 309 27
6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4432 5756 8626 9387 10415 1540
Size: 275 186 2431 1324 2870 761 1028 2301
Fragments arranged by size:
2870 2431 2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2301 2750 4026 4068 4719 5343 5478 6537
Size: 39 449 1276 42 651 624 135 1059
Cuts at: 6537 6894 7029 7242 7443 9237 9322 10393 2262
Size: 357 135 213 201 1794 85 1071 3045
Fragments arranged by size:
3045 1794 1276 1071 1059 651 624 449
357 213 201 135 135 85 42 39
BsiWI C'GTAC_G
Cuts at: 2535 2535
Size: 11176
BsmI GAATG_Cn'
Cuts at: 6325 8687 8780 6325
Size: 2362 93 8721
Fragments arranged by size:
8721 2362 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4823 4847 5618 6088 7785 7997 4823
Size: 24 771 470 1697 212 8002
Fragments arranged by size:
8002 1697 771 470 212 24
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2290 2301 2750 3875 4026 4068
Size: 399 472 28 11 449 1125 151 42
Cuts at: 4068 4165 4545 4719 5118 5292 5343 5478 5527
Size: 97 380 174 399 174 51 135 49
Cuts at: 5527 5732 6227 6336 6537 6894 6903 7029 7143
Size: 205 495 109 201 357 9 126 114
Cuts at: 7143 7242 7347 7443 7811 7979 8067 8111 8257
Size: 99 105 96 368 168 88 44 146
Cuts at: 8257 8266 8382 8495 9237 9322 10393 1391
Size: 9 116 113 742 85 1071 2174
Fragments arranged by size:
2174 1125 1071 742 495 472 449 399
399 380 368 357 205 201 174 174
168 151 146 135 126 116 114 113
109 105 99 97 96 88 85 51
49 44 42 28 11 9 9
BspEI T'CCGG_A
Cuts at: 4656 4905 4989 6383 10839 4656
Size: 249 84 1394 4456 4993
Fragments arranged by size:
4993 4456 1394 249 84
BspHI T'CATG_A
Cuts at: 7350 9065 10795 7350
Size: 1715 1730 7731
Fragments arranged by size:
7731 1730 1715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 3786 4133 4464 5127 5195 5239 6398
Size: 1223 1719 347 331 663 68 44 1159
Cuts at: 6398 7016 8250 8311 8364 8531 844
Size: 618 1234 61 53 167 3489
Fragments arranged by size:
3489 1719 1234 1223 1159 663 618 347
331 167 68 61 53 44
BsrBI CCG'CTC
Cuts at: 1394 1823 2727 4837 5752 5917 8038 9063 1394
Size: 429 904 2110 915 165 2121 1025 3507
Fragments arranged by size:
3507 2121 2110 1025 915 904 429 165
BsrDI GCAATG_nn'
Cuts at: 65 1248 2157 9671 9845 65
Size: 1183 909 7514 174 1396
Fragments arranged by size:
7514 1396 1183 909 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2886 4631 5067 5791 7868 7950 9820
Size: 110 1076 1745 436 724 2077 82 1870
Cuts at: 9820 10007 1700
Size: 187 2869
Fragments arranged by size:
2869 2077 1870 1745 1076 724 436 187
110 82
BsrGI T'GTAC_A
Cuts at: 96 3459 7188 8334 96
Size: 3363 3729 1146 2938
Fragments arranged by size:
3729 3363 2938 1146
BssHII G'CGCG_C
Cuts at: 1925 8094 1925
Size: 6169 5007
BssSI C'ACGA_G
Cuts at: 2101 3538 3811 5417 6073 9236 10248 2101
Size: 1437 273 1606 656 3163 1012 3029
Fragments arranged by size:
3163 3029 1606 1437 1012 656 273
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 4621 4846 4933 6340 6996 7252 7327
Size: 1472 2355 225 87 1407 656 256 75
Cuts at: 7327 8261 794
Size: 934 3709
Fragments arranged by size:
3709 2355 1472 1407 934 656 256 225
87 75
BstBI TT'CG_AA
Cuts at: 9980 9980
Size: 11176
BstEII G'GTnAC_C
Cuts at: 1184 3379 5548 1184
Size: 2195 2169 6812
Fragments arranged by size:
6812 2195 2169
BstXI CCAn_nnnn'nTGG
Cuts at: 3172 4765 5932 7150 7633 8405 8471 3172
Size: 1593 1167 1218 483 772 66 5877
Fragments arranged by size:
5877 1593 1218 1167 772 483 66
BstYI r'GATC_y
Cuts at: 1972 2526 5892 6675 6694 7911 8428 8506 8873
Size: 554 3366 783 19 1217 517 78 367
Cuts at: 8873 9258 9275 10727 10813 10825 11171 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
3366 1977 1452 1217 783 554 517 385
367 346 86 78 19 17 12
Bsu36I CC'TnA_GG
Cuts at: 2304 5307 2304
Size: 3003 8173
BtgI C'CryG_G
Cuts at: 513 1065 2429 3165 3444 3762 4041 4086 5400
Size: 552 1364 736 279 318 279 45 1314
Cuts at: 5400 5676 5931 6132 6805 7754 8121 8202 513
Size: 276 255 201 673 949 367 81 3487
Fragments arranged by size:
3487 1364 1314 949 736 673 552 367
318 279 279 276 255 201 81 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6380 7890 487
Size: 683 56 5154 1510 3773
Fragments arranged by size:
5154 3773 1510 683 56
BtsI GCAGTG_nn'
Cuts at: 2764 3429 4006 4098 6330 6785 6953 7042 7328
Size: 665 577 92 2232 455 168 89 286
Cuts at: 7328 8692 9486 9506 2764
Size: 1364 794 20 4434
Fragments arranged by size:
4434 2232 1364 794 665 577 455 286
168 92 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11176
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5821 5856 529
Size: 35 5257 35 5849
Fragments arranged by size:
5849 5257 35 35
DraI TTT'AAA
Cuts at: 8480 8480
Size: 11176
DraIII CAC_nnn'GTG
Cuts at: 2266 6340 7629 8261 2266
Size: 4074 1289 632 5181
Fragments arranged by size:
5181 4074 1289 632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4607 5780 10183 809
Size: 507 3291 1173 4403 1802
Fragments arranged by size:
4403 3291 1802 1173 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2556 2658 3222
Size: 54 1425 50 275 18 726 102 564
Cuts at: 3222 3447 3828 3971 4044 5740 5753 5922 5961
Size: 225 381 143 73 1696 13 169 39
Cuts at: 5961 6105 6732 6745 7090 7542 8010 8124 8205
Size: 144 627 13 345 452 468 114 81
Cuts at: 8205 8395 8623 9512 11141 8
Size: 190 228 889 1629 43
Fragments arranged by size:
1696 1629 1425 889 726 627 564 468
452 381 345 275 228 225 190 169
144 143 114 102 81 73 54 50
43 39 18 13 13
EagI C'GGCC_G
Cuts at: 1537 1812 5753 8623 1537
Size: 275 3941 2870 4090
Fragments arranged by size:
4090 3941 2870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3042 3251 3522 3776 4173 5163 6782 8382
Size: 1689 209 271 254 397 990 1619 1600
Cuts at: 8382 9106 1353
Size: 724 3423
Fragments arranged by size:
3423 1689 1619 1600 990 724 397 271
254 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 4553 5081 5235 6500 8200
Size: 1064 138 867 2097 528 154 1265 1700
Cuts at: 8200 9763 10137 10283 387
Size: 1563 374 146 1280
Fragments arranged by size:
2097 1700 1563 1280 1265 1064 867 528
374 154 146 138
EcoICRI GAG'CTC
Cuts at: 4024 6535 7240 4024
Size: 2511 705 7960
Fragments arranged by size:
7960 2511 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2657 2861 2866 3099 3155 3350
Size: 393 881 144 204 5 233 56 195
Cuts at: 3350 3620 3689 4280 4298 4661 4787 4844 4937
Size: 270 69 591 18 363 126 57 93
Cuts at: 4937 5012 5021 5119 5259 5492 5718 6317 6335
Size: 75 9 98 140 233 226 599 18
Cuts at: 6335 7172 7301 7337 7457 7490 7718 7862 7874
Size: 837 129 36 120 33 228 144 12
Cuts at: 7874 8051 8396 9239 9836 10623 10891 1239
Size: 177 345 843 597 787 268 1524
Fragments arranged by size:
1524 881 843 837 787 599 597 591
393 363 345 270 268 233 233 228
226 204 195 177 144 144 140 129
126 120 98 93 75 69 57 56
36 33 18 18 12 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 3419 4724 5038 6107 7060 7493 7508 7723
Size: 1089 1305 314 1069 953 433 15 215
Cuts at: 7723 8030 8387 2330
Size: 307 357 5119
Fragments arranged by size:
5119 1305 1089 1069 953 433 357 314
307 215 15
EcoO109I rG'GnC_Cy
Cuts at: 3130 3556 4279 5328 5362 6367 6979 7198 7795
Size: 426 723 1049 34 1005 612 219 597
Cuts at: 7795 8052 8063 8064 3130
Size: 257 11 1 6242
Fragments arranged by size:
6242 1049 1005 723 612 597 426 257
219 34 11 1
EcoRI G'AATT_C
Cuts at: 8485 8863 8485
Size: 378 10798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 3160 6841 2527
Size: 633 3681 6862
Fragments arranged by size:
6862 3681 633
FspI TGC'GCA
Cuts at: 4652 9682 4652
Size: 5030 6146
FspAI rTGC'GCAy
Cuts at: 4652 4652
Size: 11176
HaeII r_GCGC'y
Cuts at: 1678 2407 3055 3468 4491 4677 5000 5808 5931
Size: 729 648 413 1023 186 323 808 123
Cuts at: 5931 7253 7541 8293 8373 10323 1678
Size: 1322 288 752 80 1950 2531
Fragments arranged by size:
2531 1950 1322 1023 808 752 729 648
413 323 288 186 123 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2928 2931 2960 2963 3302
Size: 32 135 32 1356 3 29 3 339
Cuts at: 3302 3334 4379 4411 5092 5124 5627 5659 6179
Size: 32 1045 32 681 32 503 32 520
Cuts at: 6179 6211 6227 6259 7118 7150 9817 9849 9891
Size: 32 16 32 859 32 2667 32 42
Cuts at: 9891 9923 1373
Size: 32 2626
Fragments arranged by size:
2667 2626 1356 1045 859 681 520 503
339 135 42 32 32 32 32 32
32 32 32 32 32 32 29 16
3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3388 6235 7519 8520 8766 669
Size: 988 281 1450 2847 1284 1001 246 3079
Fragments arranged by size:
3079 2847 1450 1284 1001 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3395 748
Size: 2647 8529
HpaI GTT'AAC
Cuts at: 8766 8766
Size: 11176
KasI G'GCGC_C
Cuts at: 2403 8289 8369 2403
Size: 5886 80 5210
Fragments arranged by size:
5886 5210 80
KpnI G_GTAC'C
Cuts at: 5905 8501 5905
Size: 2596 8580
MfeI C'AATT_G
Cuts at: 8775 8775
Size: 11176
MluI A'CGCG_T
Cuts at: 11052 11052
Size: 11176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3264 5727 10290 10474 1305
Size: 1959 2463 4563 184 2007
Fragments arranged by size:
4563 2463 2007 1959 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 3449 3830 5742 5924
Size: 54 1425 343 726 891 381 1912 182
Cuts at: 5924 6107 6734 6747 7092 7544 8397 10
Size: 183 627 13 345 452 853 2789
Fragments arranged by size:
2789 1912 1425 891 853 726 627 452
381 345 343 183 182 54 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2552 3170 4031 4091 4415 4634
Size: 648 843 543 618 861 60 324 219
Cuts at: 4634 6356 7310 7631 9134 9493 9652 518
Size: 1722 954 321 1503 359 159 2042
Fragments arranged by size:
2042 1722 1503 954 861 843 648 618
543 359 324 321 219 159 60
MspA1I CmG'CkG
Cuts at: 1606 2077 2377 2626 2802 4056 4557 4621 5056
Size: 471 300 249 176 1254 501 64 435
Cuts at: 5056 5276 5899 6164 7414 8592 9269 10417 10662
Size: 220 623 265 1250 1178 677 1148 245
Cuts at: 10662 1606
Size: 2120
Fragments arranged by size:
2120 1254 1250 1178 1148 677 623 501
471 435 300 265 249 245 220 176
64
NaeI GCC'GGC
Cuts at: 7870 7870
Size: 11176
NarI GG'CG_CC
Cuts at: 2404 8290 8370 2404
Size: 5886 80 5210
Fragments arranged by size:
5886 5210 80
NcoI C'CATG_G
Cuts at: 513 1065 3165 3444 4086 5676 7754 513
Size: 552 2100 279 642 1590 2078 3935
Fragments arranged by size:
3935 2100 2078 1590 642 552 279
NdeI CA'TA_TG
Cuts at: 387 2370 387
Size: 1983 9193
NgoMIV G'CCGG_C
Cuts at: 7868 7868
Size: 11176
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11176
NotI GC'GGCC_GC
Cuts at: 8623 8623
Size: 11176
NspI r_CATG'y
Cuts at: 1132 1229 3970 4639 7528 8534 10079 1132
Size: 97 2741 669 2889 1006 1545 2229
Fragments arranged by size:
2889 2741 2229 1545 1006 669 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11176
PciI A'CATG_T
Cuts at: 10075 10075
Size: 11176
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 5677 5998 1108
Size: 1518 3051 321 6286
Fragments arranged by size:
6286 3051 1518 321
PfoI T'CCnGG_A
Cuts at: 3558 3945 4689 4764 5574 6885 7161 7434 7827
Size: 387 744 75 810 1311 276 273 393
Cuts at: 7827 3558
Size: 6907
Fragments arranged by size:
6907 1311 810 744 393 387 276 273
75
PmeI GTTT'AAAC
Cuts at: 8480 8480
Size: 11176
PmlI CAC'GTG
Cuts at: 3702 6337 6682 6904 7171 7629 3702
Size: 2635 345 222 267 458 7249
Fragments arranged by size:
7249 2635 458 345 267 222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7982 8014 9245 9277 10758 10790 7982
Size: 32 1231 32 1481 32 8368
Fragments arranged by size:
8368 1481 1231 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3130 3556 4279 5328 7795 3130
Size: 426 723 1049 2467 6511
Fragments arranged by size:
6511 2467 1049 723 426
PshAI GACnn'nnGTC
Cuts at: 1613 4814 1613
Size: 3201 7975
PsiI TTA'TAA
Cuts at: 3252 8746 3252
Size: 5494 5682
PspOMI G'GGCC_C
Cuts at: 8063 8063
Size: 11176
PspXI vC'TCGA_Gb
Cuts at: 7508 7508
Size: 11176
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 11144
PstI C_TGCA'G
Cuts at: 830 2334 4144 4617 4745 4851 4896 4938 5100
Size: 1504 1810 473 128 106 45 42 162
Cuts at: 5100 5124 6124 6409 6992 7257 7708 8528 830
Size: 24 1000 285 583 265 451 820 3478
Fragments arranged by size:
3478 1810 1504 1000 820 583 473 451
285 265 162 128 106 45 42 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11176
PvuII CAG'CTG
Cuts at: 2077 2377 2626 4621 6164 7414 2077
Size: 300 249 1995 1543 1250 5839
Fragments arranged by size:
5839 1995 1543 1250 300 249
SacI G_AGCT'C
Cuts at: 4026 6537 7242 4026
Size: 2511 705 7960
Fragments arranged by size:
7960 2511 705
SalI G'TCGA_C
Cuts at: 1936 6233 8518 1936
Size: 4297 2285 4594
Fragments arranged by size:
4594 4297 2285
SapI GCTCTTCn'nnn_
Cuts at: 3042 4173 3042
Size: 1131 10045
SbfI CC_TGCA'GG
Cuts at: 4745 4851 8528 4745
Size: 106 3677 7393
Fragments arranged by size:
7393 3677 106
ScaI AGT'ACT
Cuts at: 3863 9424 11136 3863
Size: 5561 1712 3903
Fragments arranged by size:
5561 3903 1712
SexAI A'CCwGG_T
Cuts at: 6762 6918 6762
Size: 156 11020
SfcI C'TryA_G
Cuts at: 826 1045 2330 3264 3409 4140 4613 4741 4847
Size: 219 1285 934 145 731 473 128 106
Cuts at: 4847 4892 4934 5096 5120 6120 6405 6988 7253
Size: 45 42 162 24 1000 285 583 265
Cuts at: 7253 7704 8524 9659 10340 10531 826
Size: 451 820 1135 681 191 1471
Fragments arranged by size:
1471 1285 1135 1000 934 820 731 681
583 473 451 285 265 219 191 162
145 128 106 45 42 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2666 4127 6029 2666
Size: 1461 1902 7813
Fragments arranged by size:
7813 1902 1461
SfoI GGC'GCC
Cuts at: 2405 8291 8371 2405
Size: 5886 80 5210
Fragments arranged by size:
5886 5210 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11176
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11176
SmaI CCC'GGG
Cuts at: 1872 6222 7820 8267 8347 8503 1872
Size: 4350 1598 447 80 156 4545
Fragments arranged by size:
4545 4350 1598 447 156 80
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 3068 3155 6536 7508 7616
Size: 197 926 558 567 87 3381 972 108
Cuts at: 7616 9280 10181 10443 820
Size: 1664 901 262 1553
Fragments arranged by size:
3381 1664 1553 972 926 901 567 558
262 197 108 87
SnaBI TAC'GTA
Cuts at: 493 8967 493
Size: 8474 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11176
SphI G_CATG'C
Cuts at: 1132 4639 8534 1132
Size: 3507 3895 3774
Fragments arranged by size:
3895 3774 3507
SspI AAT'ATT
Cuts at: 5 52 9100 5
Size: 47 9048 2081
Fragments arranged by size:
9048 2081 47
StuI AGG'CCT
Cuts at: 1517 2099 4967 5033 5737 7133 8229 1517
Size: 582 2868 66 704 1396 1096 4464
Fragments arranged by size:
4464 2868 1396 1096 704 582 66
StyI C'CwwG_G
Cuts at: 513 1065 2121 3165 3444 4086 4725 4812 5676
Size: 552 1056 1044 279 642 639 87 864
Cuts at: 5676 5849 6108 6276 6423 7754 7970 8615 513
Size: 173 259 168 147 1331 216 645 3074
Fragments arranged by size:
3074 1331 1056 1044 864 645 642 639
552 279 259 216 173 168 147 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2221 2813 4188 5567 9203 9220 9373
Size: 1255 641 592 1375 1379 3636 17 153
Cuts at: 9373 9558 10003 325
Size: 185 445 1498
Fragments arranged by size:
3636 1498 1379 1375 1255 641 592 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3459 3861 4680
Size: 274 80 33 51 1443 1482 402 819
Cuts at: 4680 6654 7188 8334 9422 11134 96
Size: 1974 534 1146 1088 1712 138
Fragments arranged by size:
1974 1712 1482 1443 1146 1088 819 534
402 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5489 5521 7548 7580 11136 11168 5489
Size: 32 2027 32 3556 32 5497
Fragments arranged by size:
5497 3556 2027 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3728 5226 1316
Size: 2412 1498 7266
Fragments arranged by size:
7266 2412 1498
XbaI T'CTAG_A
Cuts at: 8512 8512
Size: 11176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 3172 3232 3556 5767 2170
Size: 1002 60 324 2211 7579
Fragments arranged by size:
7579 2211 1002 324 60
XhoI C'TCGA_G
Cuts at: 1943 7508 1943
Size: 5565 5611
XmaI C'CCGG_G
Cuts at: 1870 6220 7818 8265 8345 8501 1870
Size: 4350 1598 447 80 156 4545
Fragments arranged by size:
4545 4350 1598 447 156 80
XmnI GAAnn'nnTTC
Cuts at: 5610 6824 9305 5610
Size: 1214 2481 7481
Fragments arranged by size:
7481 2481 1214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2143 6082 7612 8007
Size: 53 83 186 936 609 3939 1530 395
Cuts at: 8007 8984 276
Size: 977 2468
Fragments arranged by size:
3939 2468 1530 977 936 609 395 186
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AvrII BaeI BfrBI BstZ17I EcoRV FseI NruI
NsiI PacI RsrII SacII SanDI SrfI SwaI