Restriction map for
pFN21AB0007
(Circular) (Six-base) MAPSORT of: pFN21AB0007.seq Check: 5220 from: 1 to: 11521
pFN21AB0007 11521 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2817 3252 2817
Size: 435 11086
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9331 278
Size: 53 83 186 936 7795 2468
Fragments arranged by size:
7795 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 6414 6612 8864 1937
Size: 1227 3250 198 2252 4594
Fragments arranged by size:
4594 3250 2252 1227 198
Acc65I G'GTAC_C
Cuts at: 8842 8842
Size: 11521
AclI AA'CG_TT
Cuts at: 1563 9648 10021 1563
Size: 8085 373 3063
Fragments arranged by size:
8085 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 3018 3074 3189 3200 3368 3476 4186
Size: 785 601 56 115 11 168 108 710
Cuts at: 4186 4457 5087 5105 5270 6582 6785 7488 8204
Size: 271 630 18 165 1312 203 703 716
Cuts at: 8204 8291 9584 10968 11236 1632
Size: 87 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1312 1293 785 716 710 703
630 601 271 268 203 168 165 115
108 87 56 18 11
AfeI AGC'GCT
Cuts at: 8381 8381
Size: 11521
AflII C'TTAA_G
Cuts at: 820 1017 4130 5053 820
Size: 197 3113 923 7288
Fragments arranged by size:
7288 3113 923 197
AflIII A'CryG_T
Cuts at: 1203 2560 4350 8661 10420 11397 1203
Size: 1357 1790 4311 1759 977 1327
Fragments arranged by size:
4311 1790 1759 1357 1327 977
AgeI A'CCGG_T
Cuts at: 10352 10352
Size: 11521
AhdI GACnn_n'nnGTC
Cuts at: 2313 4093 7782 10250 2313
Size: 1780 3689 2468 3584
Fragments arranged by size:
3689 3584 2468 1780
AleI CACnn'nnGTG
Cuts at: 1166 7754 1166
Size: 6588 4933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5007 6483 1184
Size: 3823 1476 6222
Fragments arranged by size:
6222 3823 1476
AlwNI CAG_nnn'CTG
Cuts at: 2224 3244 3868 4261 5188 5701 5764 6524 7100
Size: 1020 624 393 927 513 63 760 576
Cuts at: 7100 8212 8497 9264 10836 2224
Size: 1112 285 767 1572 2909
Fragments arranged by size:
2909 1572 1112 1020 927 767 760 624
576 513 393 285 63
ApaI G_GGCC'C
Cuts at: 3497 3497
Size: 11521
ApaLI G'TGCA_C
Cuts at: 9578 10734 9578
Size: 1156 10365
ApoI r'AATT_y
Cuts at: 1493 4751 5171 5784 6547 8008 8770 8830 9056
Size: 3258 420 613 763 1461 762 60 226
Cuts at: 9056 9208 11317 1493
Size: 152 2109 1697
Fragments arranged by size:
3258 2109 1697 1461 763 762 613 420
226 152 60
AseI AT'TA_AT
Cuts at: 160 1032 6323 10075 160
Size: 872 5291 3752 1606
Fragments arranged by size:
5291 3752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11521
AvaI C'yCGr_G
Cuts at: 1870 1943 2481 3496 5136 6429 6657 6702 8698
Size: 73 538 1015 1640 1293 228 45 1996
Cuts at: 8698 8846 1870
Size: 148 4545
Fragments arranged by size:
4545 1996 1640 1293 1015 538 228 148
73 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4523 4556 4523
Size: 33 11488
BamHI G'GATC_C
Cuts at: 6727 7114 8851 9218 6727
Size: 387 1737 367 9030
Fragments arranged by size:
9030 1737 387 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2196 5549 7910 8842
Size: 325 217 627 324 85 3353 2361 932
Cuts at: 8842 10297 618
Size: 1455 1842
Fragments arranged by size:
3353 2361 1842 1455 932 627 325 324
217 85
BanII G_rGCy'C
Cuts at: 1391 2265 2458 2982 3189 3400 3497 4893 5127
Size: 874 193 524 207 211 97 1396 234
Cuts at: 5127 7530 8060 8681 8840 1391
Size: 2403 530 621 159 4072
Fragments arranged by size:
4072 2403 1396 874 621 530 524 234
211 207 193 159 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3743 4089 4353 4496 5397 5816 7526
Size: 971 1844 346 264 143 901 419 1710
Cuts at: 7526 8658 928
Size: 1132 3791
Fragments arranged by size:
3791 1844 1710 1132 971 901 419 346
264 143
BbvCI CC'TCA_GC
Cuts at: 4164 6119 7862 4164
Size: 1955 1743 7823
Fragments arranged by size:
7823 1955 1743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2723 2757 4010 4044 9712 9746 1903
Size: 34 786 34 1253 34 5668 34 3678
Fragments arranged by size:
5668 3678 1253 786 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4551 9413 10623 3461
Size: 1090 4862 1210 4359
Fragments arranged by size:
4862 4359 1210 1090
BclI T'GATC_A
Cuts at: 1548 2145 2376 3534 7840 1548
Size: 597 231 1158 4306 5229
Fragments arranged by size:
5229 4306 1158 597 231
BfrBI ATG'CAT
Cuts at: 7506 7506
Size: 11521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2670 3501 3605
Size: 107 122 71 631 605 998 831 104
Cuts at: 3605 10132 136
Size: 6527 1525
Fragments arranged by size:
6527 1525 998 831 631 605 122 107
104 71
BglII A'GATC_T
Cuts at: 5030 5080 11516 5030
Size: 50 6436 5035
Fragments arranged by size:
6436 5035 50
BlpI GC'TnA_GC
Cuts at: 5262 5283 8938 5262
Size: 21 3655 7845
Fragments arranged by size:
7845 3655 21
Bme1580I G_kGCm'C
Cuts at: 1790 2114 3497 4321 8820 9582 10738 1790
Size: 324 1383 824 4499 762 1156 2573
Fragments arranged by size:
4499 2573 1383 1156 824 762 324
BmgBI CAC'GTC
Cuts at: 1318 2841 1318
Size: 1523 9998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5209 7227 7338
Size: 416 53 83 399 24 3792 2018 111
Cuts at: 7338 8610 9252 10210 442
Size: 1272 642 958 1753
Fragments arranged by size:
3792 2018 1753 1272 958 642 416 399
111 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2279 2956 7438 8116 2279
Size: 677 4482 678 5684
Fragments arranged by size:
5684 4482 678 677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2917 3173 3287 4310 4667 4877 5055 5261
Size: 1678 256 114 1023 357 210 178 206
Cuts at: 5261 5693 5723 5786 5978 6285 6374 6953 7094
Size: 432 30 63 192 307 89 579 141
Cuts at: 7094 8111 8180 8408 10181 1239
Size: 1017 69 228 1773 2579
Fragments arranged by size:
2579 1773 1678 1023 1017 579 432 357
307 256 228 210 206 192 178 141
114 89 69 63 30
Bpu10I CC'TnA_GC
Cuts at: 4164 6119 7862 8166 4164
Size: 1955 1743 304 7519
Fragments arranged by size:
7519 1955 1743 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3773 7150 9646 10511 10809 3773
Size: 3377 2496 865 298 4485
Fragments arranged by size:
4485 3377 2496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6056 6886 6998 10184 882
Size: 617 4557 830 112 3186 2219
Fragments arranged by size:
4557 3186 2219 830 617 112
BsaAI yAC'GTr
Cuts at: 493 1204 3487 6757 9312 493
Size: 711 2283 3270 2555 2702
Fragments arranged by size:
3270 2702 2555 2283 711
BsaBI GATnn'nnATC
Cuts at: 3114 4686 6390 6681 11515 3114
Size: 1572 1704 291 4834 3120
Fragments arranged by size:
4834 3120 1704 1572 291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8760 9328 9710 10345
Size: 53 83 186 936 7227 568 382 635
Cuts at: 10345 275
Size: 1451
Fragments arranged by size:
7227 1451 936 635 568 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6566 8460 8711 9954 10352 10626 10773 11184 6566
Size: 1894 251 1243 398 274 147 411 6903
Fragments arranged by size:
6903 1894 1243 411 398 274 251 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2750 2780 2840
Size: 30 165 30 603 30 714 30 60
Cuts at: 2840 2870 2873 2903 5492 5522 5966 5996 7874
Size: 30 3 30 2589 30 444 30 1878
Cuts at: 7874 7904 8216 8246 8381 8400 8411 8430 1178
Size: 30 312 30 135 19 11 19 4269
Fragments arranged by size:
4269 2589 1878 714 603 444 312 165
135 60 30 30 30 30 30 30
30 30 30 30 19 19 11 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 2777 2888 2891 2894 3534 4397 5135
Size: 1588 3 111 3 3 640 863 738
Cuts at: 5135 5258 5846 1186
Size: 123 588 6861
Fragments arranged by size:
6861 1588 863 738 640 588 123 111
3 3 3
BseYI C'CCAG_C
Cuts at: 2541 3894 4509 8648 10724 11220 2541
Size: 1353 615 4139 2076 496 2842
Fragments arranged by size:
4139 2842 2076 1353 615 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2291 3329 4160 5965 6270 6779 6796
Size: 573 555 1038 831 1805 305 509 17
Cuts at: 6796 7586 8158 1163
Size: 790 572 4526
Fragments arranged by size:
4526 1805 1038 831 790 573 572 555
509 305 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8971 9732 10760 1540
Size: 275 186 6970 761 1028 2301
Fragments arranged by size:
6970 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2982 3400 4047 4893 5127 9582 9667 10738
Size: 717 418 647 846 234 4455 85 1071
Cuts at: 10738 2265
Size: 3048
Fragments arranged by size:
4455 3048 1071 846 717 647 418 234
85
BsmI GAATG_Cn'
Cuts at: 6576 9032 9125 6576
Size: 2456 93 8972
Fragments arranged by size:
8972 2456 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2265 2458 2982 3189 3400 3497
Size: 399 324 151 193 524 207 211 97
Cuts at: 3497 4047 4321 4893 5127 7530 8060 8681 8820
Size: 550 274 572 234 2403 530 621 139
Cuts at: 8820 8840 9582 9667 10738 1391
Size: 20 742 85 1071 2174
Fragments arranged by size:
2403 2174 1071 742 621 572 550 530
524 399 324 274 234 211 207 193
151 139 97 85 20
BspEI T'CCGG_A
Cuts at: 8460 8711 11184 8460
Size: 251 2473 8797
Fragments arranged by size:
8797 2473 251
BspHI T'CATG_A
Cuts at: 9410 11140 9410
Size: 1730 9791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2736 2784 2817 3252 3947 5196 8540
Size: 1244 648 48 33 435 695 1249 3344
Cuts at: 8540 8876 844
Size: 336 3489
Fragments arranged by size:
3489 3344 1249 1244 695 648 435 336
48 33
BsrBI CCG'CTC
Cuts at: 1394 1823 8122 8685 8697 9408 1394
Size: 429 6299 563 12 711 3507
Fragments arranged by size:
6299 3507 711 563 429 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 5450 6144 8061 10016 10190 65
Size: 1183 4202 694 1917 1955 174 1396
Fragments arranged by size:
4202 1955 1917 1396 1183 694 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2180 10165 10352 1700
Size: 110 370 7985 187 2869
Fragments arranged by size:
7985 2869 370 187 110
BsrGI T'GTAC_A
Cuts at: 96 3340 96
Size: 3244 8277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11521
BssSI C'ACGA_G
Cuts at: 9581 10593 9581
Size: 1012 10509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3244 794
Size: 2450 9071
BstBI TT'CG_AA
Cuts at: 10325 10325
Size: 11521
BstEII G'GTnAC_C
Cuts at: 1184 2092 7134 1184
Size: 908 5042 5571
Fragments arranged by size:
5571 5042 908
BstXI CCAn_nnnn'nTGG
Cuts at: 7756 7756
Size: 11521
BstYI r'GATC_y
Cuts at: 1972 4515 5030 5080 6254 6727 7114 7426 8702
Size: 2543 515 50 1174 473 387 312 1276
Cuts at: 8702 8714 8851 9218 9603 9620 11072 11158 11170
Size: 12 137 367 385 17 1452 86 12
Cuts at: 11170 11516 1972
Size: 346 1977
Fragments arranged by size:
2543 1977 1452 1276 1174 515 473 387
385 367 346 312 137 86 50 17
12 12
Bsu36I CC'TnA_GG
Cuts at: 4912 6282 7589 4912
Size: 1370 1307 8844
Fragments arranged by size:
8844 1370 1307
BtgI C'CryG_G
Cuts at: 513 1065 2030 3561 7294 8199 8583 513
Size: 552 965 1531 3733 905 384 3451
Fragments arranged by size:
3733 3451 1531 965 905 552 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2902 7956 487
Size: 683 56 1676 5054 4052
Fragments arranged by size:
5054 4052 1676 683 56
BtsI GCAGTG_nn'
Cuts at: 3080 4045 4319 7936 8349 8740 9037 9831 9851
Size: 965 274 3617 413 391 297 794 20
Cuts at: 9851 3080
Size: 4750
Fragments arranged by size:
4750 3617 965 794 413 391 297 274
20
ClaI AT'CG_AT
Cuts at: 1554 6685 1554
Size: 5131 6390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2491 2526 529
Size: 35 1927 35 9524
Fragments arranged by size:
9524 1927 35 35
DraI TTT'AAA
Cuts at: 8825 8825
Size: 11521
DraIII CAC_nnn'GTG
Cuts at: 3102 3102
Size: 11521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3011 10528 809
Size: 507 1695 7517 1802
Fragments arranged by size:
7517 1802 1695 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3558 4479 5308
Size: 54 1425 50 275 18 1728 921 829
Cuts at: 5308 8259 8374 8479 8968 9857 11486 8
Size: 2951 115 105 489 889 1629 43
Fragments arranged by size:
2951 1728 1629 1425 921 889 829 489
275 115 105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8968 1537
Size: 275 7156 4090
Fragments arranged by size:
7156 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 3632 5063 5112 5246 5552 5931 6815
Size: 1794 485 1431 49 134 306 379 884
Cuts at: 6815 9451 1353
Size: 2636 3423
Fragments arranged by size:
3423 2636 1794 1431 884 485 379 306
134 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2618 10108 10482 10628 387
Size: 1064 138 1029 7490 374 146 1280
Fragments arranged by size:
7490 1280 1064 1029 374 146 138
EcoICRI GAG'CTC
Cuts at: 2263 2980 3398 4891 5125 2263
Size: 717 418 1493 234 8659
Fragments arranged by size:
8659 1493 717 418 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2917 3018 3074 3173 3189 3200
Size: 393 785 500 101 56 99 16 11
Cuts at: 3200 3287 3368 3476 4186 4310 4457 4667 4877
Size: 87 81 108 710 124 147 210 210
Cuts at: 4877 5055 5087 5105 5261 5270 5693 5723 5786
Size: 178 32 18 156 9 423 30 63
Cuts at: 5786 5978 6285 6374 6582 6785 6953 7094 7488
Size: 192 307 89 208 203 168 141 394
Cuts at: 7488 8111 8180 8204 8291 8408 9584 10181 10968
Size: 623 69 24 87 117 1176 597 787
Cuts at: 10968 11236 1239
Size: 268 1524
Fragments arranged by size:
1524 1176 787 785 710 623 597 500
423 394 393 307 268 210 210 208
203 192 178 168 156 147 141 124
117 108 101 99 89 87 87 81
69 63 56 32 30 24 18 16
11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2794 7236 2794
Size: 4442 7079
EcoO109I rG'GnC_Cy
Cuts at: 4642 6051 6433 7663 7816 8727 8739 4642
Size: 1409 382 1230 153 911 12 7424
Fragments arranged by size:
7424 1409 1230 911 382 153 12
EcoRI G'AATT_C
Cuts at: 8770 8830 9208 8770
Size: 60 378 11083
Fragments arranged by size:
11083 378 60
EcoRV GAT'ATC
Cuts at: 6011 8596 6011
Size: 2585 8936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4208 5246 7493 4208
Size: 1038 2247 8236
Fragments arranged by size:
8236 2247 1038
FspI TGC'GCA
Cuts at: 10027 10027
Size: 11521
HaeII r_GCGC'y
Cuts at: 1678 5764 6043 8383 10668 1678
Size: 4086 279 2340 2285 2531
Fragments arranged by size:
4086 2531 2340 2285 279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2144 2176 2299
Size: 32 135 32 454 32 86 32 123
Cuts at: 2299 2331 2624 2656 2788 2820 3146 3178 3270
Size: 32 293 32 132 32 326 32 92
Cuts at: 3270 3302 3526 3558 4079 4111 4507 4539 4660
Size: 32 224 32 521 32 396 32 121
Cuts at: 4660 4692 5132 5164 5261 5293 5966 5998 7768
Size: 32 440 32 97 32 673 32 1770
Cuts at: 7768 7800 7874 7906 8216 8248 10162 10194 10236
Size: 32 74 32 310 32 1914 32 42
Cuts at: 10236 10268 1373
Size: 32 2626
Fragments arranged by size:
2626 1914 1770 673 521 454 440 396
326 310 293 224 135 132 123 121
97 92 86 74 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2957 3508 3952 6091 6415 7372
Size: 988 281 1019 551 444 2139 324 957
Cuts at: 7372 7615 7825 8134 8419 8865 9111 669
Size: 243 210 309 285 446 246 3079
Fragments arranged by size:
3079 2139 1019 988 957 551 446 444
324 309 285 281 246 243 210
HindIII A'AGCT_T
Cuts at: 748 4419 5228 7019 8752 748
Size: 3671 809 1791 1733 3517
Fragments arranged by size:
3671 3517 1791 1733 809
HpaI GTT'AAC
Cuts at: 9111 9111
Size: 11521
KpnI G_GTAC'C
Cuts at: 8846 8846
Size: 11521
MfeI C'AATT_G
Cuts at: 4158 4664 9120 4158
Size: 506 4456 6559
Fragments arranged by size:
6559 4456 506
MluI A'CGCG_T
Cuts at: 11397 11397
Size: 11521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 2736 3062 4788 7081 10635 10819 1305
Size: 960 471 326 1726 2293 3554 184 2007
Fragments arranged by size:
3554 2293 2007 1726 960 471 326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4481 8261 8376 10
Size: 54 1425 343 2649 3780 115 3155
Fragments arranged by size:
3780 3155 2649 1425 343 115 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2948 3632 4112 4772 6344 6396 7754
Size: 648 1782 684 480 660 1572 52 1358
Cuts at: 7754 9479 9838 9997 518
Size: 1725 359 159 2042
Fragments arranged by size:
2042 1782 1725 1572 1358 684 660 648
480 359 159 52
MspA1I CmG'CkG
Cuts at: 1606 2032 2100 2707 2818 3346 4428 4591 4602
Size: 426 68 607 111 528 1082 163 11
Cuts at: 4602 4897 6160 6763 8937 9614 10762 11007 1606
Size: 295 1263 603 2174 677 1148 245 2120
Fragments arranged by size:
2174 2120 1263 1148 1082 677 607 603
528 426 295 245 163 111 68 11
NcoI C'CATG_G
Cuts at: 513 1065 8583 513
Size: 552 7518 3451
Fragments arranged by size:
7518 3451 552
NdeI CA'TA_TG
Cuts at: 387 6046 387
Size: 5659 5862
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11521
NotI GC'GGCC_GC
Cuts at: 8968 8968
Size: 11521
NsiI A_TGCA'T
Cuts at: 7508 7508
Size: 11521
NspI r_CATG'y
Cuts at: 1132 1229 2564 3232 3514 4354 6832 8665 8879
Size: 97 1335 668 282 840 2478 1833 214
Cuts at: 8879 10424 1132
Size: 1545 2229
Fragments arranged by size:
2478 2229 1833 1545 1335 840 668 282
214 97
PasI CC'CwG_GG
Cuts at: 1348 8236 1348
Size: 6888 4633
PciI A'CATG_T
Cuts at: 2560 4350 8661 10420 2560
Size: 1790 4311 1759 3661
Fragments arranged by size:
4311 3661 1790 1759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3868 6794 8257 1108
Size: 2760 2926 1463 4372
Fragments arranged by size:
4372 2926 2760 1463
PfoI T'CCnGG_A
Cuts at: 2217 3189 5175 5670 8385 8741 2217
Size: 972 1986 495 2715 356 4997
Fragments arranged by size:
4997 2715 1986 972 495 356
PmeI GTTT'AAAC
Cuts at: 8825 8825
Size: 11521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9590 9622 11103 11135 9590
Size: 32 1481 32 9976
Fragments arranged by size:
9976 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4642 6051 6433 8727 8739 4642
Size: 1409 382 2294 12 7424
Fragments arranged by size:
7424 2294 1409 382 12
PshAI GACnn'nnGTC
Cuts at: 1613 3869 1613
Size: 2256 9265
PsiI TTA'TAA
Cuts at: 2463 9091 2463
Size: 6628 4893
PspOMI G'GGCC_C
Cuts at: 3493 3493
Size: 11521
PspXI vC'TCGA_Gb
Cuts at: 2481 2481
Size: 11521
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 11489
PstI C_TGCA'G
Cuts at: 830 2232 2823 3069 3240 4023 4996 5193 5433
Size: 1402 591 246 171 783 973 197 240
Cuts at: 5433 5541 5697 7204 8873 830
Size: 108 156 1507 1669 3478
Fragments arranged by size:
3478 1669 1507 1402 973 783 591 246
240 197 171 156 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11521
PvuII CAG'CTG
Cuts at: 2707 2818 3346 4591 4897 6160 6763 2707
Size: 111 528 1245 306 1263 603 7465
Fragments arranged by size:
7465 1263 1245 603 528 306 111
SacI G_AGCT'C
Cuts at: 2265 2982 3400 4893 5127 2265
Size: 717 418 1493 234 8659
Fragments arranged by size:
8659 1493 717 418 234
SacII CC_GC'GG
Cuts at: 2033 2033
Size: 11521
SalI G'TCGA_C
Cuts at: 1936 6413 8863 1936
Size: 4477 2450 4594
Fragments arranged by size:
4594 4477 2450
SanDI GG'GwC_CC
Cuts at: 6051 8727 8739 6051
Size: 2676 12 8833
Fragments arranged by size:
8833 2676 12
SapI GCTCTTCn'nnn_
Cuts at: 3632 3632
Size: 11521
SbfI CC_TGCA'GG
Cuts at: 8873 8873
Size: 11521
ScaI AGT'ACT
Cuts at: 3590 4220 9769 11481 3590
Size: 630 5549 1712 3630
Fragments arranged by size:
5549 3630 1712 630
SexAI A'CCwGG_T
Cuts at: 7060 7060
Size: 11521
SfcI C'TryA_G
Cuts at: 826 1045 2228 2819 3065 3236 3367 4019 4499
Size: 219 1183 591 246 171 131 652 480
Cuts at: 4499 4992 5189 5429 5537 5693 6369 7200 8869
Size: 493 197 240 108 156 676 831 1669
Cuts at: 8869 10004 10685 10876 826
Size: 1135 681 191 1471
Fragments arranged by size:
1669 1471 1183 1135 831 681 676 652
591 493 480 246 240 219 197 191
171 156 131 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11521
SmaI CCC'GGG
Cuts at: 1872 3498 6659 8848 1872
Size: 1626 3161 2189 4545
Fragments arranged by size:
4545 3161 2189 1626
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2481 3752 4130 5053 7129 9625
Size: 197 926 538 1271 378 923 2076 2496
Cuts at: 9625 10526 10788 820
Size: 901 262 1553
Fragments arranged by size:
2496 2076 1553 1271 926 923 901 538
378 262 197
SnaBI TAC'GTA
Cuts at: 493 9312 493
Size: 8819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11521
SphI G_CATG'C
Cuts at: 1132 8879 1132
Size: 7747 3774
SspI AAT'ATT
Cuts at: 5 52 5819 9445 5
Size: 47 5767 3626 2081
Fragments arranged by size:
5767 3626 2081 47
StuI AGG'CCT
Cuts at: 1517 2936 3050 7652 8171 1517
Size: 1419 114 4602 519 4867
Fragments arranged by size:
4867 4602 1419 519 114
StyI C'CwwG_G
Cuts at: 513 1065 2502 3787 5461 6177 6651 6838 8583
Size: 552 1437 1285 1674 716 474 187 1745
Cuts at: 8583 8637 8960 513
Size: 54 323 3074
Fragments arranged by size:
3074 1745 1674 1437 1285 716 552 474
323 187 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 8208 9548 9565 9718 9903 10348 325
Size: 1255 6628 1340 17 153 185 445 1498
Fragments arranged by size:
6628 1498 1340 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 3340 3588
Size: 274 80 33 51 1443 147 1216 248
Cuts at: 3588 4218 4843 5621 6814 9767 11479 96
Size: 630 625 778 1193 2953 1712 138
Fragments arranged by size:
2953 1712 1443 1216 1193 778 630 625
274 248 147 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2840 2872 4663 4695 11481 11513 2840
Size: 32 1791 32 6786 32 2848
Fragments arranged by size:
6786 2848 1791 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5980 8228 1316
Size: 4664 2248 4609
Fragments arranged by size:
4664 4609 2248
XbaI T'CTAG_A
Cuts at: 5293 5734 8857 5293
Size: 441 3123 7957
Fragments arranged by size:
7957 3123 441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2575 7097 7759 7921 2353
Size: 222 4522 662 162 5953
Fragments arranged by size:
5953 4522 662 222 162
XhoI C'TCGA_G
Cuts at: 1943 2481 1943
Size: 538 10983
XmaI C'CCGG_G
Cuts at: 1870 3496 6657 8846 1870
Size: 1626 3161 2189 4545
Fragments arranged by size:
4545 3161 2189 1626
XmnI GAAnn'nnTTC
Cuts at: 5171 8288 8468 9650 5171
Size: 3117 180 1182 7042
Fragments arranged by size:
7042 3117 1182 180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9329 276
Size: 53 83 186 936 7795 2468
Fragments arranged by size:
7795 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
RsrII SfiI SfoI SrfI SwaI