Restriction map for
pFN21AB0006
(Circular) (Six-base) MAPSORT of: pFN21AB0006.seq Check: 7626 from: 1 to: 11500
pFN21AB0006 11500 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3648 3648
Size: 11500
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9310 278
Size: 53 83 186 936 7774 2468
Fragments arranged by size:
7774 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2949 3678 4032 4415 5454 6027 6506 8843
Size: 1012 729 354 383 1039 573 479 2337
Cuts at: 8843 1937
Size: 4594
Fragments arranged by size:
4594 2337 1039 1012 729 573 479 383
354
Acc65I G'GTAC_C
Cuts at: 8821 8821
Size: 11500
AclI AA'CG_TT
Cuts at: 1563 9627 10000 1563
Size: 8064 373 3063
Fragments arranged by size:
8064 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2298 2304 2993 4037 5385 5943 6178 6938
Size: 666 6 689 1044 1348 558 235 760
Cuts at: 6938 7682 9563 10947 11215 1632
Size: 744 1881 1384 268 1917
Fragments arranged by size:
1917 1881 1384 1348 1044 760 744 689
666 558 268 235 6
AflII C'TTAA_G
Cuts at: 820 1017 7118 820
Size: 197 6101 5202
Fragments arranged by size:
6101 5202 197
AflIII A'CryG_T
Cuts at: 1203 2493 3494 8471 8522 10399 11376 1203
Size: 1290 1001 4977 51 1877 977 1327
Fragments arranged by size:
4977 1877 1327 1290 1001 977 51
AgeI A'CCGG_T
Cuts at: 10331 10331
Size: 11500
AhdI GACnn_n'nnGTC
Cuts at: 3864 4243 10229 3864
Size: 379 5986 5135
Fragments arranged by size:
5986 5135 379
AleI CACnn'nnGTG
Cuts at: 1166 2828 4118 4325 5294 5312 5570 6195 1166
Size: 1662 1290 207 969 18 258 625 6471
Fragments arranged by size:
6471 1662 1290 969 625 258 207 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2440 6156 1184
Size: 1256 3716 6528
Fragments arranged by size:
6528 3716 1256
AlwNI CAG_nnn'CTG
Cuts at: 2053 2971 3701 4300 4436 4864 6347 6874 7364
Size: 918 730 599 136 428 1483 527 490
Cuts at: 7364 9243 10815 2053
Size: 1879 1572 2738
Fragments arranged by size:
2738 1879 1572 1483 918 730 599 527
490 428 136
ApaI G_GGCC'C
Cuts at: 2025 5071 8014 2025
Size: 3046 2943 5511
Fragments arranged by size:
5511 3046 2943
ApaLI G'TGCA_C
Cuts at: 3490 6131 9557 10713 3490
Size: 2641 3426 1156 4277
Fragments arranged by size:
4277 3426 2641 1156
ApoI r'AATT_y
Cuts at: 1493 3244 3949 5659 6235 8495 8809 9035 9187
Size: 1751 705 1710 576 2260 314 226 152
Cuts at: 9187 11296 1493
Size: 2109 1697
Fragments arranged by size:
2260 2109 1751 1710 1697 705 576 314
226 152
AseI AT'TA_AT
Cuts at: 160 1032 6612 10054 160
Size: 872 5580 3442 1606
Fragments arranged by size:
5580 3442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11500
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 3234 5064 6147 6185 6207 8793
Size: 73 81 1210 1830 1083 38 22 2586
Cuts at: 8793 8825 1870
Size: 32 4545
Fragments arranged by size:
4545 2586 1830 1210 1083 81 73 38
32 22
AvrII C'CTAG_G
Cuts at: 2212 2212
Size: 11500
BamHI G'GATC_C
Cuts at: 6171 8830 9197 6171
Size: 2659 367 8474
Fragments arranged by size:
8474 2659 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 3254 4703 5383 6220
Size: 325 217 627 278 1189 1449 680 837
Cuts at: 6220 8135 8391 8582 8821 10276 618
Size: 1915 256 191 239 1455 1842
Fragments arranged by size:
1915 1842 1455 1449 1189 837 680 627
325 278 256 239 217 191
BanII G_rGCy'C
Cuts at: 1391 2025 5071 5231 5273 5696 5869 7195 7412
Size: 634 3046 160 42 423 173 1326 217
Cuts at: 7412 7686 8014 8819 1391
Size: 274 328 805 4072
Fragments arranged by size:
4072 3046 1326 805 634 423 328 274
217 173 160 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2981 6067 7022 8546 928
Size: 971 1082 3086 955 1524 3882
Fragments arranged by size:
3882 3086 1524 1082 971 955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2855 2889 3537 3571 8171
Size: 34 50 34 834 34 648 34 4600
Cuts at: 8171 8205 9691 9725 1903
Size: 34 1486 34 3678
Fragments arranged by size:
4600 3678 1486 834 648 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5075 5206 9392 10602 5075
Size: 131 4186 1210 5973
Fragments arranged by size:
5973 4186 1210 131
BclI T'GATC_A
Cuts at: 1548 2566 5316 5686 6399 1548
Size: 1018 2750 370 713 6649
Fragments arranged by size:
6649 2750 1018 713 370
BfrBI ATG'CAT
Cuts at: 3528 3528
Size: 11500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8619 10111 136
Size: 107 122 71 631 605 6947 1492 1525
Fragments arranged by size:
6947 1525 1492 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7374 7761 11495 7374
Size: 387 3734 7379
Fragments arranged by size:
7379 3734 387
BlpI GC'TnA_GC
Cuts at: 2103 8917 2103
Size: 6814 4686
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2070 3494 3621 4728 5071 6135 6225
Size: 235 45 1424 127 1107 343 1064 90
Cuts at: 6225 8014 9561 10717 1790
Size: 1789 1547 1156 2573
Fragments arranged by size:
2573 1789 1547 1424 1156 1107 1064 343
235 127 90 45
BmgBI CAC'GTC
Cuts at: 1318 4765 1318
Size: 3447 8053
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 2319 3711
Size: 416 53 83 399 24 699 203 1392
Cuts at: 3711 6949 7860 8655 9231 10189 442
Size: 3238 911 795 576 958 1753
Fragments arranged by size:
3238 1753 1392 958 911 795 699 576
416 399 203 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2118 4983 5073 6066 6888 2118
Size: 2865 90 993 822 6730
Fragments arranged by size:
6730 2865 993 822 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2485 2756 2761 4516 4997 5262 5421 5570
Size: 1246 271 5 1755 481 265 159 149
Cuts at: 5570 5589 5747 6004 6246 6555 7187 7255 10160
Size: 19 158 257 242 309 632 68 2905
Cuts at: 10160 1239
Size: 2579
Fragments arranged by size:
2905 2579 1755 1246 632 481 309 271
265 257 242 159 158 149 68 19
5
Bpu10I CC'TnA_GC
Cuts at: 2009 6947 7146 8347 2009
Size: 4938 199 1201 5162
Fragments arranged by size:
5162 4938 1201 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 9625 10490 10788 3811
Size: 5814 865 298 4523
Fragments arranged by size:
5814 4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2464 3934 4222 4231 5297 6196 7208
Size: 617 965 1470 288 9 1066 899 1012
Cuts at: 7208 7563 10163 882
Size: 355 2600 2219
Fragments arranged by size:
2600 2219 1470 1066 1012 965 899 617
355 288 9
BsaAI yAC'GTr
Cuts at: 493 1204 3006 3495 7514 8551 9291 493
Size: 711 1802 489 4019 1037 740 2702
Fragments arranged by size:
4019 2702 1802 1037 740 711 489
BsaBI GATnn'nnATC
Cuts at: 11494 11494
Size: 11500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5327 7428 9307 9689
Size: 53 83 186 936 3794 2101 1879 382
Cuts at: 9689 10324 275
Size: 635 1451
Fragments arranged by size:
3794 2101 1879 1451 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7019 9933 10331 10605 10752 11163 7019
Size: 2914 398 274 147 411 7356
Fragments arranged by size:
7356 2914 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3844 3874 3980 4010 5317
Size: 30 165 30 2441 30 106 30 1307
Cuts at: 5317 5347 5851 5881 6718 6748 1178
Size: 30 504 30 837 30 5930
Fragments arranged by size:
5930 2441 1307 837 504 165 106 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2482 2814 2901 3252 4152 4292 5391 5608
Size: 1296 332 87 351 900 140 1099 217
Cuts at: 5608 5799 6135 7551 1186
Size: 191 336 1416 5135
Fragments arranged by size:
5135 1416 1296 1099 900 351 336 332
217 191 140 87
BseYI C'CCAG_C
Cuts at: 2387 2576 2904 5353 5797 6405 10703 11199 2387
Size: 189 328 2449 444 608 4298 496 2688
Fragments arranged by size:
4298 2688 2449 608 496 444 328 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2767 2852 2895 3109 3181 3190 3397
Size: 573 1031 85 43 214 72 9 207
Cuts at: 3397 4821 5683 6888 8452 8581 1163
Size: 1424 862 1205 1564 129 4082
Fragments arranged by size:
4082 1564 1424 1205 1031 862 573 214
207 129 85 72 43 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2797 8950 9711 10739 1540
Size: 275 186 796 6153 761 1028 2301
Fragments arranged by size:
6153 2301 1028 796 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3494 4854 5273 5577 5600 5696 6047 6135 6192
Size: 1360 419 304 23 96 351 88 57
Cuts at: 6192 7412 9561 9646 10717 3494
Size: 1220 2149 85 1071 4277
Fragments arranged by size:
4277 2149 1360 1220 1071 419 351 304
96 88 85 57 23
BsmI GAATG_Cn'
Cuts at: 3901 9011 9104 3901
Size: 5110 93 6297
Fragments arranged by size:
6297 5110 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4139 7244 4139
Size: 3105 8395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2070 3494 3621 4728 4854 5071
Size: 399 235 45 1424 127 1107 126 217
Cuts at: 5071 5231 5273 5577 5600 5696 5869 6047 6135
Size: 160 42 304 23 96 173 178 88
Cuts at: 6135 6192 6225 7195 7412 7686 8014 8819 9561
Size: 57 33 970 217 274 328 805 742
Cuts at: 9561 9646 10717 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1424 1107 1071 970 805 742 399
328 304 274 235 217 217 178 173
160 127 126 96 88 85 57 45
42 33 23
BspEI T'CCGG_A
Cuts at: 11163 11163
Size: 11500
BspHI T'CATG_A
Cuts at: 3607 8512 9389 11119 3607
Size: 4905 877 1730 3988
Fragments arranged by size:
4905 3988 1730 877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2384 2546 2775 3648 4350 4749 4848 5829
Size: 1540 162 229 873 702 399 99 981
Cuts at: 5829 6764 7467 8855 844
Size: 935 703 1388 3489
Fragments arranged by size:
3489 1540 1388 981 935 873 703 702
399 229 162 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2203 2847 9387 1394
Size: 429 380 644 6540 3507
Fragments arranged by size:
6540 3507 644 429 380
BsrDI GCAATG_nn'
Cuts at: 65 1248 2224 3528 3796 7653 9995 10169 65
Size: 1183 976 1304 268 3857 2342 174 1396
Fragments arranged by size:
3857 2342 1396 1304 1183 976 268 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4808 7526 10144 10331 1700
Size: 110 2998 2718 2618 187 2869
Fragments arranged by size:
2998 2869 2718 2618 187 110
BsrGI T'GTAC_A
Cuts at: 96 3632 7383 8770 96
Size: 3536 3751 1387 2826
Fragments arranged by size:
3751 3536 2826 1387
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11500
BssSI C'ACGA_G
Cuts at: 3401 5201 5388 7194 8254 9560 10572 3401
Size: 1800 187 1806 1060 1306 1012 4329
Fragments arranged by size:
4329 1806 1800 1306 1060 1012 187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2676 4864 794
Size: 1882 2188 7430
Fragments arranged by size:
7430 2188 1882
BstBI TT'CG_AA
Cuts at: 3173 10304 3173
Size: 7131 4369
BstEII G'GTnAC_C
Cuts at: 1184 4771 4915 7000 7726 1184
Size: 3587 144 2085 726 4958
Fragments arranged by size:
4958 3587 2085 726 144
BstXI CCAn_nnnn'nTGG
Cuts at: 3377 5899 7573 8402 3377
Size: 2522 1674 829 6475
Fragments arranged by size:
6475 2522 1674 829
BstYI r'GATC_y
Cuts at: 1972 2849 4303 6171 7374 7761 8830 9197 9582
Size: 877 1454 1868 1203 387 1069 367 385
Cuts at: 9582 9599 11051 11137 11149 11495 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1868 1454 1452 1203 1069 877 387
385 367 346 86 17 12
BstZ17I GTA'TAC
Cuts at: 4033 4033
Size: 11500
BtgI C'CryG_G
Cuts at: 513 1065 2073 3390 3778 4948 5094 6574 7062
Size: 552 1008 1317 388 1170 146 1480 488
Cuts at: 7062 8208 513
Size: 1146 3805
Fragments arranged by size:
3805 1480 1317 1170 1146 1008 552 488
388 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2398 487
Size: 683 56 1172 9589
Fragments arranged by size:
9589 1172 683 56
BtsI GCAGTG_nn'
Cuts at: 2972 4809 4947 5255 5976 7434 9016 9810 9830
Size: 1837 138 308 721 1458 1582 794 20
Cuts at: 9830 2972
Size: 4642
Fragments arranged by size:
4642 1837 1582 1458 794 721 308 138
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7423 7458 529
Size: 35 6859 35 4571
Fragments arranged by size:
6859 4571 35 35
DraI TTT'AAA
Cuts at: 8329 8804 8329
Size: 475 11025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4769 5524 10507 809
Size: 507 3453 755 4983 1802
Fragments arranged by size:
4983 3453 1802 755 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2794 2920
Size: 54 1425 50 275 18 210 754 126
Cuts at: 2920 3775 4066 4170 4351 5205 5647 6328 6571
Size: 855 291 104 181 854 442 681 243
Cuts at: 6571 7086 7291 7470 7929 8452 8680 8947 9836
Size: 515 205 179 459 523 228 267 889
Cuts at: 9836 11465 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 855 854 754 681 523
515 459 442 291 275 267 243 228
210 205 181 179 126 104 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2794 8947 1537
Size: 275 982 6153 4090
Fragments arranged by size:
6153 4090 982 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2280 3316 3606 5104 7670 8190 9430
Size: 728 199 1036 290 1498 2566 520 1240
Cuts at: 9430 1353
Size: 3423
Fragments arranged by size:
3423 2566 1498 1240 1036 728 520 290
199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6164 7924 10087 10461 10607 387
Size: 1064 138 4575 1760 2163 374 146 1280
Fragments arranged by size:
4575 2163 1760 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5271 5694 7410 5271
Size: 423 1716 9361
Fragments arranged by size:
9361 1716 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2298 2304 2485 2756 2761 2993 4037
Size: 393 666 6 181 271 5 232 1044
Cuts at: 4037 4516 4997 5262 5385 5421 5570 5589 5747
Size: 479 481 265 123 36 149 19 158
Cuts at: 5747 5943 6004 6178 6246 6555 6938 7187 7255
Size: 196 61 174 68 309 383 249 68
Cuts at: 7255 7682 9563 10160 10947 11215 1239
Size: 427 1881 597 787 268 1524
Fragments arranged by size:
1881 1524 1044 787 666 597 481 479
427 393 383 309 271 268 265 249
232 196 181 174 158 149 123 68
68 61 36 19 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 4732 5590 6054 4732
Size: 858 464 10178
Fragments arranged by size:
10178 858 464
EcoO109I rG'GnC_Cy
Cuts at: 2021 4738 5138 6361 8011 2021
Size: 2717 400 1223 1650 5510
Fragments arranged by size:
5510 2717 1650 1223 400
EcoRI G'AATT_C
Cuts at: 6235 8495 8809 9187 6235
Size: 2260 314 378 8548
Fragments arranged by size:
8548 2260 378 314
EcoRV GAT'ATC
Cuts at: 4383 6989 4383
Size: 2606 8894
FspI TGC'GCA
Cuts at: 10006 10006
Size: 11500
HaeII r_GCGC'y
Cuts at: 1678 4436 6954 8629 10647 1678
Size: 2758 2518 1675 2018 2531
Fragments arranged by size:
2758 2531 2518 2018 1675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3697 3729 3850 3882 4229
Size: 32 135 32 2125 32 121 32 347
Cuts at: 4229 4261 4463 4495 5047 5079 5317 5349 5678
Size: 32 202 32 552 32 238 32 329
Cuts at: 5678 5710 6130 6141 6162 6163 6173 6195 6623
Size: 32 420 11 21 1 10 22 428
Cuts at: 6623 6655 7043 7075 7436 7468 7560 7592 7746
Size: 32 388 32 361 32 92 32 154
Cuts at: 7746 7778 8698 8730 10141 10173 10215 10247 1373
Size: 32 920 32 1411 32 42 32 2626
Fragments arranged by size:
2626 2125 1411 920 552 428 420 388
361 347 329 238 202 154 135 121
92 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 22 21 11 10 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3679 4837 8224 8844 9090 669
Size: 988 281 1741 1158 3387 620 246 3079
Fragments arranged by size:
3387 3079 1741 1158 988 620 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11500
HpaI GTT'AAC
Cuts at: 9090 9090
Size: 11500
KpnI G_GTAC'C
Cuts at: 8825 8825
Size: 11500
MfeI C'AATT_G
Cuts at: 4482 5499 8745 9099 4482
Size: 1017 3246 354 6883
Fragments arranged by size:
6883 3246 1017 354
MluI A'CGCG_T
Cuts at: 11376 11376
Size: 11500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2575 3944 4260 7479 8272 10614 10798 1305
Size: 1270 1369 316 3219 793 2342 184 2007
Fragments arranged by size:
3219 2342 2007 1369 1270 793 316 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2922 4172 5207 6330 7088
Size: 54 1425 343 1090 1250 1035 1123 758
Cuts at: 7088 7293 7472 8454 8682 10
Size: 205 179 982 228 2828
Fragments arranged by size:
2828 1425 1250 1123 1090 1035 982 758
343 228 205 179 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 4118 4325 4778 5294 5312 5420
Size: 648 1662 1290 207 453 516 18 108
Cuts at: 5420 5570 6195 6554 8153 8386 8400 9458 9817
Size: 150 625 359 1599 233 14 1058 359
Cuts at: 9817 9976 518
Size: 159 2042
Fragments arranged by size:
2042 1662 1599 1290 1058 648 625 516
453 359 359 233 207 159 150 108
18 14
MspA1I CmG'CkG
Cuts at: 1606 2063 2664 2879 3027 3041 5801 6405 6522
Size: 457 601 215 148 14 2760 604 117
Cuts at: 6522 8617 8916 9593 10741 10986 1606
Size: 2095 299 677 1148 245 2120
Fragments arranged by size:
2760 2120 2095 1148 677 604 601 457
299 245 215 148 117 14
NcoI C'CATG_G
Cuts at: 513 1065 3390 4948 513
Size: 552 2325 1558 7065
Fragments arranged by size:
7065 2325 1558 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11500
NotI GC'GGCC_GC
Cuts at: 8947 8947
Size: 11500
NsiI A_TGCA'T
Cuts at: 3530 3530
Size: 11500
NspI r_CATG'y
Cuts at: 1132 1229 2497 3528 3625 3639 8475 8604 8858
Size: 97 1268 1031 97 14 4836 129 254
Cuts at: 8858 10403 1132
Size: 1545 2229
Fragments arranged by size:
4836 2229 1545 1268 1031 254 129 97
97 14
PasI CC'CwG_GG
Cuts at: 1348 4734 4910 7811 1348
Size: 3386 176 2901 5037
Fragments arranged by size:
5037 3386 2901 176
PciI A'CATG_T
Cuts at: 2493 8471 10399 2493
Size: 5978 1928 3594
Fragments arranged by size:
5978 3594 1928
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2053 2364 3891 4782 5342 7233 7289 8007
Size: 945 311 1527 891 560 1891 56 718
Cuts at: 8007 1108
Size: 4601
Fragments arranged by size:
4601 1891 1527 945 891 718 560 311
56
PfoI T'CCnGG_A
Cuts at: 5437 6477 8019 8092 5437
Size: 1040 1542 73 8845
Fragments arranged by size:
8845 1542 1040 73
PmeI GTTT'AAAC
Cuts at: 8804 8804
Size: 11500
PmlI CAC'GTG
Cuts at: 3006 3495 7514 3006
Size: 489 4019 6992
Fragments arranged by size:
6992 4019 489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9569 9601 11082 11114 9569
Size: 32 1481 32 9955
Fragments arranged by size:
9955 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4738 5138 6361 4738
Size: 400 1223 9877
Fragments arranged by size:
9877 1223 400
PshAI GACnn'nnGTC
Cuts at: 1613 3474 1613
Size: 1861 9639
PsiI TTA'TAA
Cuts at: 9070 9070
Size: 11500
PspOMI G'GGCC_C
Cuts at: 2021 5067 8010 2021
Size: 3046 2943 5511
Fragments arranged by size:
5511 3046 2943
PspXI vC'TCGA_Gb
Cuts at: 3234 6147 3234
Size: 2913 8587
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7624 7656 7624
Size: 32 11468
PstI C_TGCA'G
Cuts at: 830 4746 5175 5262 6775 6889 8852 830
Size: 3916 429 87 1513 114 1963 3478
Fragments arranged by size:
3916 3478 1963 1513 429 114 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11500
PvuII CAG'CTG
Cuts at: 2879 3027 3041 5801 6405 2879
Size: 148 14 2760 604 7974
Fragments arranged by size:
7974 2760 604 148 14
SacI G_AGCT'C
Cuts at: 5273 5696 7412 5273
Size: 423 1716 9361
Fragments arranged by size:
9361 1716 423
SalI G'TCGA_C
Cuts at: 1936 3677 8842 1936
Size: 1741 5165 4594
Fragments arranged by size:
5165 4594 1741
SanDI GG'GwC_CC
Cuts at: 5138 5138
Size: 11500
SapI GCTCTTCn'nnn_
Cuts at: 2081 7670 2081
Size: 5589 5911
SbfI CC_TGCA'GG
Cuts at: 8852 8852
Size: 11500
ScaI AGT'ACT
Cuts at: 9748 11460 9748
Size: 1712 9788
SexAI A'CCwGG_T
Cuts at: 4618 4618
Size: 11500
SfcI C'TryA_G
Cuts at: 826 1045 2722 3954 4742 5171 5258 6166 6507
Size: 219 1677 1232 788 429 87 908 341
Cuts at: 6507 6771 6885 8848 9983 10664 10855 826
Size: 264 114 1963 1135 681 191 1471
Fragments arranged by size:
1963 1677 1471 1232 1135 908 788 681
429 341 264 219 191 114 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11500
SmaI CCC'GGG
Cuts at: 1872 2026 8827 1872
Size: 154 6801 4545
Fragments arranged by size:
6801 4545 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3234 3826 6147 6207 7118 9604
Size: 197 926 1291 592 2321 60 911 2486
Cuts at: 9604 10505 10767 820
Size: 901 262 1553
Fragments arranged by size:
2486 2321 1553 1291 926 911 901 592
262 197 60
SnaBI TAC'GTA
Cuts at: 493 9291 493
Size: 8798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11500
SphI G_CATG'C
Cuts at: 1132 8858 1132
Size: 7726 3774
SrfI GCCC'GGGC
Cuts at: 2026 2026
Size: 11500
SspI AAT'ATT
Cuts at: 5 52 9424 5
Size: 47 9372 2081
Fragments arranged by size:
9372 2081 47
StuI AGG'CCT
Cuts at: 1517 4014 8124 1517
Size: 2497 4110 4893
Fragments arranged by size:
4893 4110 2497
StyI C'CwwG_G
Cuts at: 513 1065 2212 2620 3390 4948 7008 8939 513
Size: 552 1147 408 770 1558 2060 1931 3074
Fragments arranged by size:
3074 2060 1931 1558 1147 770 552 408
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 3632 4205 5345 6671 6952 6982
Size: 1255 738 1314 573 1140 1326 281 30
Cuts at: 6982 7495 7820 9527 9544 9697 9882 10327 325
Size: 513 325 1707 17 153 185 445 1498
Fragments arranged by size:
1707 1498 1326 1314 1255 1140 738 573
513 445 325 281 185 153 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2231 2304 2642
Size: 274 80 33 51 1443 254 73 338
Cuts at: 2642 3632 3924 4670 5127 5187 5730 7383 8034
Size: 990 292 746 457 60 543 1653 651
Cuts at: 8034 8770 9746 11458 96
Size: 736 976 1712 138
Fragments arranged by size:
1712 1653 1443 990 976 746 736 651
543 457 338 292 274 254 138 80
73 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3496 3528 3844 3876 3980 4012 4242 4274 5851
Size: 32 316 32 104 32 230 32 1577
Cuts at: 5851 5883 6978 7010 7068 7100 8240 8272 11460
Size: 32 1095 32 58 32 1140 32 3188
Cuts at: 11460 11492 3496
Size: 32 3504
Fragments arranged by size:
3504 3188 1577 1140 1095 316 230 104
58 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11500
XbaI T'CTAG_A
Cuts at: 2684 8384 8836 2684
Size: 5700 452 5348
Fragments arranged by size:
5700 5348 452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3056 4210 7489 8288 3056
Size: 1154 3279 799 6268
Fragments arranged by size:
6268 3279 1154 799
XhoI C'TCGA_G
Cuts at: 1943 3234 6147 6207 1943
Size: 1291 2913 60 7236
Fragments arranged by size:
7236 2913 1291 60
XmaI C'CCGG_G
Cuts at: 1870 2024 8825 1870
Size: 154 6801 4545
Fragments arranged by size:
6801 4545 154
XmnI GAAnn'nnTTC
Cuts at: 9629 9629
Size: 11500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9308 276
Size: 53 83 186 936 7774 2468
Fragments arranged by size:
7774 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BsiWI Bsu36I
DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI RsrII SacII SfiI SfoI SwaI