Restriction map for
pFN21AA2034
(Circular) (Six-base) MAPSORT of: pFN21AA2034.seq Check: 5860 from: 1 to: 10813
pFN21AA2034 10813 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 3798 4705 2134
Size: 1664 907 8242
Fragments arranged by size:
8242 1664 907
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6747 8623 278
Size: 53 83 186 936 5211 1876 2468
Fragments arranged by size:
5211 2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3837 8156 1937
Size: 1900 4319 4594
Fragments arranged by size:
4594 4319 1900
Acc65I G'GTAC_C
Cuts at: 8134 8134
Size: 10813
AclI AA'CG_TT
Cuts at: 1563 8940 9313 1563
Size: 7377 373 3063
Fragments arranged by size:
7377 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2729 3793 4550 5366 5735 7085 7844 8876
Size: 1097 1064 757 816 369 1350 759 1032
Cuts at: 8876 10260 10528 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1350 1097 1064 1032 816 759
757 369 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10616
AflIII A'CryG_T
Cuts at: 1203 2409 2523 9712 10689 1203
Size: 1206 114 7189 977 1327
Fragments arranged by size:
7189 1327 1206 977 114
AgeI A'CCGG_T
Cuts at: 3009 9644 3009
Size: 6635 4178
AhdI GACnn_n'nnGTC
Cuts at: 5560 5916 9542 5560
Size: 356 3626 6831
Fragments arranged by size:
6831 3626 356
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4036 6773 1184
Size: 2852 2737 5224
Fragments arranged by size:
5224 2852 2737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3689 3721 7542 7574 3689
Size: 32 3821 32 6928
Fragments arranged by size:
6928 3821 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2950 4010 4438 4759 4936 5083 5335 5506 5767
Size: 1060 428 321 177 147 252 171 261
Cuts at: 5767 5791 6145 6400 6463 6547 7546 8556 10128
Size: 24 354 255 63 84 999 1010 1572
Cuts at: 10128 2950
Size: 3635
Fragments arranged by size:
3635 1572 1060 1010 999 428 354 321
261 255 252 177 171 147 84 63
24
ApaI G_GGCC'C
Cuts at: 2060 2106 4753 5428 5494 5500 6634 6703 7603
Size: 46 2647 675 66 6 1134 69 900
Cuts at: 7603 2060
Size: 5270
Fragments arranged by size:
5270 2647 1134 900 675 69 66 46
6
ApaLI G'TGCA_C
Cuts at: 8870 10026 8870
Size: 1156 9657
ApoI r'AATT_y
Cuts at: 1493 2819 5123 5158 8122 8348 8500 10609 1493
Size: 1326 2304 35 2964 226 152 2109 1697
Fragments arranged by size:
2964 2304 2109 1697 1326 226 152 35
AseI AT'TA_AT
Cuts at: 160 1032 9367 160
Size: 872 8335 1606
Fragments arranged by size:
8335 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10813
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2066 2128 2990 3264 4569 5493
Size: 73 87 36 62 862 274 1305 924
Cuts at: 5493 6186 6372 6627 7179 7860 8138 1870
Size: 693 186 255 552 681 278 4545
Fragments arranged by size:
4545 1305 924 862 693 681 552 278
274 255 186 87 73 62 36
BamHI G'GATC_C
Cuts at: 8143 8510 8143
Size: 367 10446
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2041 2132 2176 2512
Size: 325 217 627 239 15 91 44 336
Cuts at: 2512 2861 3437 3786 5940 6312 6360 7386 8134
Size: 349 576 349 2154 372 48 1026 748
Cuts at: 8134 9589 618
Size: 1455 1842
Fragments arranged by size:
2154 1842 1455 1026 748 627 576 372
349 349 336 325 239 217 91 48
44 15
BanII G_rGCy'C
Cuts at: 1391 2060 2106 2997 4753 4812 5372 5428 5494
Size: 669 46 891 1756 59 560 56 66
Cuts at: 5494 5500 5682 6034 6634 6703 7603 7867 8132
Size: 6 182 352 600 69 900 264 265
Cuts at: 8132 1391
Size: 4072
Fragments arranged by size:
4072 1756 900 891 669 600 560 352
265 264 182 69 66 59 56 46
6
BbeI G_GCGC'C
Cuts at: 2045 2865 3441 5944 6316 6364 7390 2045
Size: 820 576 2503 372 48 1026 5468
Fragments arranged by size:
5468 2503 1026 820 576 372 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2621 5157 5484 5726 5855 7999 928
Size: 971 722 2536 327 242 129 2144 3742
Fragments arranged by size:
3742 2536 2144 971 722 327 242 129
BbvCI CC'TCA_GC
Cuts at: 5061 5226 5768 6207 7239 7656 5061
Size: 165 542 439 1032 417 8218
Fragments arranged by size:
8218 1032 542 439 417 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3772 3806 9004 9038 1903
Size: 34 1835 34 5198 34 3678
Fragments arranged by size:
5198 3678 1835 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4568 8705 9915 4568
Size: 4137 1210 5466
Fragments arranged by size:
5466 4137 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10813
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 2101 2657
Size: 107 122 71 631 605 378 51 556
Cuts at: 2657 4853 5867 6203 6377 6707 7227 9424 136
Size: 2196 1014 336 174 330 520 2197 1525
Fragments arranged by size:
2197 2196 1525 1014 631 605 556 520
378 336 330 174 122 107 71 51
BglII A'GATC_T
Cuts at: 3477 3936 4479 7188 10808 3477
Size: 459 543 2709 3620 3482
Fragments arranged by size:
3620 3482 2709 543 459
BlpI GC'TnA_GC
Cuts at: 5168 6107 6711 6728 7503 8230 5168
Size: 939 604 17 775 727 7751
Fragments arranged by size:
7751 939 775 727 604 17
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2060 2067 2106 2135 4753 5428 5494
Size: 241 29 7 39 29 2618 675 66
Cuts at: 5494 5500 6001 6337 6634 6652 6703 7603 7609
Size: 6 501 336 297 18 51 900 6
Cuts at: 7609 8874 10030 1790
Size: 1265 1156 2573
Fragments arranged by size:
2618 2573 1265 1156 900 675 501 336
297 241 66 51 39 29 29 18
7 6 6
BmgBI CAC'GTC
Cuts at: 1318 5918 1318
Size: 4600 6213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2054 3254 4191
Size: 416 53 83 399 24 637 1200 937
Cuts at: 4191 8544 9502 442
Size: 4353 958 1753
Fragments arranged by size:
4353 1753 1200 958 937 637 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10813
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3016 3290 7210 2987
Size: 29 274 3920 6590
Fragments arranged by size:
6590 3920 274 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2616 2625 2750 2906 2982 3095 3131 3368
Size: 1377 9 125 156 76 113 36 237
Cuts at: 3368 3587 4268 4313 4796 4958 4979 5111 5357
Size: 219 681 45 483 162 21 132 246
Cuts at: 5357 5579 5642 5663 5747 5834 5885 5969 6065
Size: 222 63 21 84 87 51 84 96
Cuts at: 6065 6254 6359 6452 6506 6536 6674 6704 6737
Size: 189 105 93 54 30 138 30 33
Cuts at: 6737 6851 6893 7064 7076 7253 7382 7400 7433
Size: 114 42 171 12 177 129 18 33
Cuts at: 7433 7730 7814 7856 9473 1239
Size: 297 84 42 1617 2579
Fragments arranged by size:
2579 1617 1377 681 483 297 246 237
222 219 189 177 171 162 156 138
132 129 125 114 113 105 96 93
87 84 84 84 76 63 54 51
45 42 42 36 33 33 30 30
21 21 18 12 9
Bpu10I CC'TnA_GC
Cuts at: 4160 5061 5226 5768 6207 7239 7343 7656 7866
Size: 901 165 542 439 1032 104 313 210
Cuts at: 7866 4160
Size: 7107
Fragments arranged by size:
7107 1032 901 542 439 313 210 165
104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4643 5075 5511 6878 7208 7655 8938 9803 10101
Size: 432 436 1367 330 447 1283 865 298
Cuts at: 10101 4643
Size: 5355
Fragments arranged by size:
5355 1367 1283 865 447 436 432 330
298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2867 3139 4518 6152 6410 7670 9476
Size: 617 1368 272 1379 1634 258 1260 1806
Cuts at: 9476 882
Size: 2219
Fragments arranged by size:
2219 1806 1634 1379 1368 1260 617 272
258
BsaAI yAC'GTr
Cuts at: 493 1204 2412 2524 2656 6674 8604 493
Size: 711 1208 112 132 4018 1930 2702
Fragments arranged by size:
4018 2702 1930 1208 711 132 112
BsaBI GATnn'nnATC
Cuts at: 10807 10807
Size: 10813
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2164 2862 3438
Size: 53 83 186 936 509 122 698 576
Cuts at: 3438 5941 6313 6361 6471 6744 7387 7576 8620
Size: 2503 372 48 110 273 643 189 1044
Cuts at: 8620 9002 9637 275
Size: 382 635 1451
Fragments arranged by size:
2503 1451 1044 936 698 643 635 576
509 382 372 273 189 186 122 110
83 53 48
BsaWI w'CCGG_w
Cuts at: 3009 4128 5682 9246 9644 9918 10065 10476 3009
Size: 1119 1554 3564 398 274 147 411 3346
Fragments arranged by size:
3564 3346 1554 1119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 2789 2819 3689
Size: 30 165 30 996 30 360 30 870
Cuts at: 3689 3719 4970 5000 5669 5699 6038 6068 1178
Size: 30 1251 30 669 30 339 30 5923
Fragments arranged by size:
5923 1251 996 870 669 360 339 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2246 2794 2975 2978 3590 3710 4532
Size: 1057 3 548 181 3 612 120 822
Cuts at: 4532 4853 4913 4991 4994 4997 5078 5243 5246
Size: 321 60 78 3 3 81 165 3
Cuts at: 5246 5249 5330 5429 5459 5462 5630 5744 5747
Size: 3 81 99 30 3 168 114 3
Cuts at: 5747 6170 6251 6254 6335 6338 6365 6569 6671
Size: 423 81 3 81 3 27 204 102
Cuts at: 6671 6674 6692 7013 7073 7115 7178 7199 7379
Size: 3 18 321 60 42 63 21 180
Cuts at: 7379 7430 7433 7601 7673 7769 7772 7853 7862
Size: 51 3 168 72 96 3 81 9
Cuts at: 7862 7865 8033 8054 1186
Size: 3 168 21 3945
Fragments arranged by size:
3945 1057 822 612 548 423 321 321
204 181 180 168 168 168 165 120
114 102 99 96 81 81 81 81
81 78 72 63 60 60 51 42
30 27 21 21 18 9 3 3
3 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2076 2105 2609 2950 4493 4506 5427 5431 5499
Size: 29 504 341 1543 13 921 4 68
Cuts at: 5499 6123 6291 6393 7296 7595 10016 10512 2076
Size: 624 168 102 903 299 2421 496 2377
Fragments arranged by size:
2421 2377 1543 921 903 624 504 496
341 299 168 102 68 29 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2575 2944 3201 3329 4042 4790
Size: 573 618 221 369 257 128 713 748
Cuts at: 4790 4908 5008 5780 5898 5990 6113 6905 1163
Size: 118 100 772 118 92 123 792 5071
Fragments arranged by size:
5071 792 772 748 713 618 573 369
257 221 128 123 118 118 100 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3659 3782 6852 7876 7920 8263
Size: 275 186 1658 123 3070 1024 44 343
Cuts at: 8263 9024 10052 1540
Size: 761 1028 2301
Fragments arranged by size:
3070 2301 1658 1028 1024 761 343 275
186 123 44
BsiHKAI G_wGCw'C
Cuts at: 3917 4812 5372 5682 6512 6817 7285 7746 8874
Size: 895 560 310 830 305 468 461 1128
Cuts at: 8874 8959 10030 3917
Size: 85 1071 4700
Fragments arranged by size:
4700 1128 1071 895 830 560 468 461
310 305 85
BsmI GAATG_Cn'
Cuts at: 8324 8417 8324
Size: 93 10720
BsmBI CGTCTCn'nnnn_
Cuts at: 2171 2670 3062 4555 4895 2171
Size: 499 392 1493 340 8089
Fragments arranged by size:
8089 1493 499 392 340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2060 2067 2106 2135 2997 3917
Size: 399 241 29 7 39 29 862 920
Cuts at: 3917 4753 4812 5372 5428 5494 5500 5682 6001
Size: 836 59 560 56 66 6 182 319
Cuts at: 6001 6034 6337 6512 6634 6652 6703 6817 7285
Size: 33 303 175 122 18 51 114 468
Cuts at: 7285 7603 7609 7746 7867 8132 8874 8959 10030
Size: 318 6 137 121 265 742 85 1071
Cuts at: 10030 1391
Size: 2174
Fragments arranged by size:
2174 1071 920 862 836 742 560 468
399 319 318 303 265 241 182 175
137 122 121 114 85 66 59 56
51 39 33 29 29 18 7 6
6
BspEI T'CCGG_A
Cuts at: 4128 10476 4128
Size: 6348 4465
BspHI T'CATG_A
Cuts at: 8702 10432 8702
Size: 1730 9083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2354 2889 3636 3798 4705 5145 6924
Size: 1290 220 535 747 162 907 440 1779
Cuts at: 6924 7462 8168 844
Size: 538 706 3489
Fragments arranged by size:
3489 1779 1290 907 747 706 538 535
440 220 162
BsrBI CCG'CTC
Cuts at: 1394 1823 2259 2386 2734 3052 3655 5179 6005
Size: 429 436 127 348 318 603 1524 826
Cuts at: 6005 6436 6595 6688 6991 7003 7533 7762 8700
Size: 431 159 93 303 12 530 229 938
Cuts at: 8700 1394
Size: 3507
Fragments arranged by size:
3507 1524 938 826 603 530 436 431
429 348 318 303 229 159 127 93
12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2782 3159 7623 9308 9482 65
Size: 1183 1534 377 4464 1685 174 1396
Fragments arranged by size:
4464 1685 1534 1396 1183 377 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3009 3658 3921 6447 7085 7871 7956
Size: 110 1199 649 263 2526 638 786 85
Cuts at: 7956 9457 9644 1700
Size: 1501 187 2869
Fragments arranged by size:
2869 2526 1501 1199 786 649 638 263
187 110 85
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10813
BssHII G'CGCG_C
Cuts at: 1925 4843 1925
Size: 2918 7895
BssSI C'ACGA_G
Cuts at: 2168 2507 3104 4226 5747 6000 8873 9885 2168
Size: 339 597 1122 1521 253 2873 1012 3096
Fragments arranged by size:
3096 2873 1521 1122 1012 597 339 253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4768 5083 5968 794
Size: 3974 315 885 5639
Fragments arranged by size:
5639 3974 885 315
BstBI TT'CG_AA
Cuts at: 9617 9617
Size: 10813
BstEII G'GTnAC_C
Cuts at: 1184 4534 7337 1184
Size: 3350 2803 4660
Fragments arranged by size:
4660 3350 2803
BstXI CCAn_nnnn'nTGG
Cuts at: 6484 6484
Size: 10813
BstYI r'GATC_y
Cuts at: 1972 2289 3477 3936 4007 4365 4479 7188 7557
Size: 317 1188 459 71 358 114 2709 369
Cuts at: 7557 8143 8510 8895 8912 10364 10450 10462 10808
Size: 586 367 385 17 1452 86 12 346
Cuts at: 10808 1972
Size: 1977
Fragments arranged by size:
2709 1977 1452 1188 586 459 385 369
367 358 346 317 114 86 71 17
12
Bsu36I CC'TnA_GG
Cuts at: 4625 8085 4625
Size: 3460 7353
BtgI C'CryG_G
Cuts at: 513 1065 2004 2098 2279 4253 5779 6198 6420
Size: 552 939 94 181 1974 1526 419 222
Cuts at: 6420 6477 7023 513
Size: 57 546 4303
Fragments arranged by size:
4303 1974 1526 939 552 546 419 222
181 94 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3448 487
Size: 683 56 2222 7852
Fragments arranged by size:
7852 2222 683 56
BtsI GCAGTG_nn'
Cuts at: 2538 4682 4706 5763 6585 6722 8329 9123 9143
Size: 2144 24 1057 822 137 1607 794 20
Cuts at: 9143 2538
Size: 4208
Fragments arranged by size:
4208 2144 1607 1057 822 794 137 24
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10813
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10778
DraI TTT'AAA
Cuts at: 8117 8117
Size: 10813
DraIII CAC_nnn'GTG
Cuts at: 2512 4103 2512
Size: 1591 9222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3076 4532 4547 9820 809
Size: 507 1760 1456 15 5273 1802
Fragments arranged by size:
5273 1802 1760 1456 507 15
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 3043 3295
Size: 54 1425 50 275 18 504 709 252
Cuts at: 3295 3357 3656 3660 3760 3779 4105 4173 5202
Size: 62 299 4 100 19 326 68 1029
Cuts at: 5202 5466 5538 5574 5782 6849 6960 7020 7087
Size: 264 72 36 208 1067 111 60 67
Cuts at: 7087 7266 7329 7873 7917 8260 9149 10778 8
Size: 179 63 544 44 343 889 1629 43
Fragments arranged by size:
1629 1425 1067 1029 889 709 544 504
343 326 299 275 264 252 208 179
111 100 72 68 67 63 62 60
54 50 44 43 36 19 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3656 3779 6849 7873 7917 8260 1537
Size: 275 1844 123 3070 1024 44 343 4090
Fragments arranged by size:
4090 3070 1844 1024 343 275 123 44
EarI CTCTTCn'nnn_
Cuts at: 1353 3065 3164 3386 3548 4226 4343 4509 4830
Size: 1712 99 222 162 678 117 166 321
Cuts at: 4830 5009 5124 6936 7401 8743 1353
Size: 179 115 1812 465 1342 3423
Fragments arranged by size:
3423 1812 1712 1342 678 465 321 222
179 166 162 117 115 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2050 2252 2267 2894 4029 5603
Size: 1064 138 461 202 15 627 1135 1574
Cuts at: 5603 5819 6964 9400 9774 9920 387
Size: 216 1145 2436 374 146 1280
Fragments arranged by size:
2436 1574 1280 1145 1135 1064 627 461
374 216 202 146 138 15
EcoICRI GAG'CTC
Cuts at: 4810 5370 5680 4810
Size: 560 310 9943
Fragments arranged by size:
9943 560 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2616 2625 2729 2750 2906 2982 3095
Size: 393 984 9 104 21 156 76 113
Cuts at: 3095 3131 3368 3587 3793 4268 4313 4550 4796
Size: 36 237 219 206 475 45 237 246
Cuts at: 4796 4958 4979 5111 5357 5366 5579 5642 5663
Size: 162 21 132 246 9 213 63 21
Cuts at: 5663 5735 5747 5834 5885 5969 6065 6254 6359
Size: 72 12 87 51 84 96 189 105
Cuts at: 6359 6452 6506 6536 6674 6704 6737 6851 6893
Size: 93 54 30 138 30 33 114 42
Cuts at: 6893 7064 7076 7085 7253 7382 7400 7433 7730
Size: 171 12 9 168 129 18 33 297
Cuts at: 7730 7814 7844 7856 8876 9473 10260 10528 1239
Size: 84 30 12 1020 597 787 268 1524
Fragments arranged by size:
1524 1020 984 787 597 475 393 297
268 246 246 237 237 219 213 206
189 171 168 162 156 138 132 129
114 113 105 104 96 93 87 84
84 76 72 63 54 51 45 42
36 33 33 30 30 30 21 21
21 18 12 12 12 9 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 3359 4340 5309 5519 5540 5612 7346 7583
Size: 1005 981 969 210 21 72 1734 237
Cuts at: 7583 2354
Size: 5584
Fragments arranged by size:
5584 1734 1005 981 969 237 210 72
21
EcoO109I rG'GnC_Cy
Cuts at: 2544 3088 3352 4288 4498 4590 5134 5490 5554
Size: 544 264 936 210 92 544 356 64
Cuts at: 5554 5605 5788 6183 6385 6631 6700 7228 7599
Size: 51 183 395 202 246 69 528 371
Cuts at: 7599 8091 2544
Size: 492 5266
Fragments arranged by size:
5266 936 544 544 528 492 395 371
356 264 246 210 202 183 92 69
64 51
EcoRI G'AATT_C
Cuts at: 5158 8122 8500 5158
Size: 2964 378 7471
Fragments arranged by size:
7471 2964 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4501 4501
Size: 10813
FseI GG_CCGG'CC
Cuts at: 3662 7875 3662
Size: 4213 6600
FspI TGC'GCA
Cuts at: 7712 9319 7712
Size: 1607 9206
HaeII r_GCGC'y
Cuts at: 1678 2045 2213 2506 2865 2961 3379 3441 4439
Size: 367 168 293 359 96 418 62 998
Cuts at: 4439 4606 4623 4794 5602 5630 5779 5944 6065
Size: 167 17 171 808 28 149 165 121
Cuts at: 6065 6316 6364 6400 7222 7390 9960 1678
Size: 251 48 36 822 168 2570 2531
Fragments arranged by size:
2570 2531 998 822 808 418 367 359
293 251 171 168 168 167 165 149
121 96 62 48 36 28 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 2789 2821 4350
Size: 32 135 32 992 32 193 32 1529
Cuts at: 4350 4382 5198 5230 5546 5578 5669 5701 5902
Size: 32 816 32 316 32 91 32 201
Cuts at: 5902 5934 6038 6062 6070 6094 6409 6441 7674
Size: 32 104 24 8 24 315 32 1233
Cuts at: 7674 7706 7841 7873 9454 9486 9528 9560 1373
Size: 32 135 32 1581 32 42 32 2626
Fragments arranged by size:
2626 1581 1529 1233 992 816 316 315
201 193 135 135 104 91 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3838 8157 8403 669
Size: 988 281 1900 4319 246 3079
Fragments arranged by size:
4319 3079 1900 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10813
HpaI GTT'AAC
Cuts at: 8403 8403
Size: 10813
KasI G'GCGC_C
Cuts at: 2041 2861 3437 5940 6312 6360 7386 2041
Size: 820 576 2503 372 48 1026 5468
Fragments arranged by size:
5468 2503 1026 820 576 372 48
KpnI G_GTAC'C
Cuts at: 8138 8138
Size: 10813
MfeI C'AATT_G
Cuts at: 4862 8412 4862
Size: 3550 7263
MluI A'CGCG_T
Cuts at: 10689 10689
Size: 10813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7380 8035 8058 9927 10111 1305
Size: 6075 655 23 1869 184 2007
Fragments arranged by size:
6075 2007 1869 655 184 23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3359 4175 5468 5576 6962
Size: 54 1425 343 1527 816 1293 108 1386
Cuts at: 6962 7331 10
Size: 369 3492
Fragments arranged by size:
3492 1527 1425 1386 1293 816 369 343
108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 3119 3560 6909 8771 9130 9289
Size: 648 858 1095 441 3349 1862 359 159
Cuts at: 9289 518
Size: 2042
Fragments arranged by size:
3349 2042 1862 1095 858 648 441 359
159
MspA1I CmG'CkG
Cuts at: 1606 2100 2281 2740 2947 3215 3387 3487 3778
Size: 494 181 459 207 268 172 100 291
Cuts at: 3778 4209 4255 4510 4565 4639 4831 5089 5225
Size: 431 46 255 55 74 192 258 136
Cuts at: 5225 5407 5431 5503 5812 5935 5959 6127 6232
Size: 182 24 72 309 123 24 168 105
Cuts at: 6232 6248 6496 6566 6848 6958 6970 7378 7502
Size: 16 248 70 282 110 12 408 124
Cuts at: 7502 7546 7792 7822 7834 8229 8906 10054 10299
Size: 44 246 30 12 395 677 1148 245
Cuts at: 10299 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 494 459 431 408 395
309 291 282 268 258 255 248 246
245 207 192 182 181 172 168 136
124 123 110 105 100 74 72 70
55 46 44 30 24 24 16 12
12
NaeI GCC'GGC
Cuts at: 3660 6449 7087 7873 3660
Size: 2789 638 786 6600
Fragments arranged by size:
6600 2789 786 638
NarI GG'CG_CC
Cuts at: 2042 2862 3438 5941 6313 6361 7387 2042
Size: 820 576 2503 372 48 1026 5468
Fragments arranged by size:
5468 2503 1026 820 576 372 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 6198 6477 513
Size: 552 939 4194 279 4849
Fragments arranged by size:
4849 4194 939 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10813
NgoMIV G'CCGG_C
Cuts at: 3658 6447 7085 7871 3658
Size: 2789 638 786 6600
Fragments arranged by size:
6600 2789 786 638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10813
NotI GC'GGCC_GC
Cuts at: 8260 8260
Size: 10813
NspI r_CATG'y
Cuts at: 1132 1229 2560 8171 9716 1132
Size: 97 1331 5611 1545 2229
Fragments arranged by size:
5611 2229 1545 1331 97
PasI CC'CwG_GG
Cuts at: 1348 5791 1348
Size: 4443 6370
PciI A'CATG_T
Cuts at: 9712 9712
Size: 10813
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2950 3855 4103 4438 5335 5791 6400 6547
Size: 1842 905 248 335 897 456 609 147
Cuts at: 6547 1108
Size: 5374
Fragments arranged by size:
5374 1842 905 897 609 456 335 248
147
PfoI T'CCnGG_A
Cuts at: 2379 3063 3456 4290 4341 5145 7560 7782 2379
Size: 684 393 834 51 804 2415 222 5410
Fragments arranged by size:
5410 2415 834 804 684 393 222 51
PmeI GTTT'AAAC
Cuts at: 8117 8117
Size: 10813
PmlI CAC'GTG
Cuts at: 2524 2656 6674 2524
Size: 132 4018 6663
Fragments arranged by size:
6663 4018 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 8882 8914 10395 10427 3689
Size: 32 5161 32 1481 32 4075
Fragments arranged by size:
5161 4075 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3088 4288 4498 5134 5554 5605 6183 8091 3088
Size: 1200 210 636 420 51 578 1908 5810
Fragments arranged by size:
5810 1908 1200 636 578 420 210 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10813
PsiI TTA'TAA
Cuts at: 8383 8383
Size: 10813
PspOMI G'GGCC_C
Cuts at: 2056 2102 4749 5424 5490 5496 6630 6699 7599
Size: 46 2647 675 66 6 1134 69 900
Cuts at: 7599 2056
Size: 5270
Fragments arranged by size:
5270 2647 1134 900 675 69 66 46
6
PspXI vC'TCGA_Gb
Cuts at: 2990 2990
Size: 10813
PstI C_TGCA'G
Cuts at: 830 2565 3537 3633 4015 4716 4743 4764 5088
Size: 1735 972 96 382 701 27 21 324
Cuts at: 5088 5382 5457 5910 5964 6027 6153 6279 6456
Size: 294 75 453 54 63 126 126 177
Cuts at: 6456 6921 7257 7473 7890 8165 830
Size: 465 336 216 417 275 3478
Fragments arranged by size:
3478 1735 972 701 465 453 417 382
336 324 294 275 216 177 126 126
96 75 63 54 27 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10813
PvuII CAG'CTG
Cuts at: 2740 2947 3215 3487 4510 4565 4831 5089 5225
Size: 207 268 272 1023 55 266 258 136
Cuts at: 5225 5431 5503 5812 5959 6127 6232 6248 6566
Size: 206 72 309 147 168 105 16 318
Cuts at: 6566 6958 6970 7378 7502 7546 7792 7822 7834
Size: 392 12 408 124 44 246 30 12
Cuts at: 7834 2740
Size: 5719
Fragments arranged by size:
5719 1023 408 392 318 309 272 268
266 258 246 207 206 168 147 136
124 105 72 55 44 30 16 12
12
RsrII CG'GwC_CG
Cuts at: 7276 7276
Size: 10813
SacI G_AGCT'C
Cuts at: 4812 5372 5682 4812
Size: 560 310 9943
Fragments arranged by size:
9943 560 310
SacII CC_GC'GG
Cuts at: 2101 2282 4256 2101
Size: 181 1974 8658
Fragments arranged by size:
8658 1974 181
SalI G'TCGA_C
Cuts at: 1936 3836 8155 1936
Size: 1900 4319 4594
Fragments arranged by size:
4594 4319 1900
SanDI GG'GwC_CC
Cuts at: 6183 8091 6183
Size: 1908 8905
SapI GCTCTTCn'nnn_
Cuts at: 3548 7401 3548
Size: 3853 6960
SbfI CC_TGCA'GG
Cuts at: 6921 7473 8165 6921
Size: 552 692 9569
Fragments arranged by size:
9569 692 552
ScaI AGT'ACT
Cuts at: 9061 10773 9061
Size: 1712 9101
SexAI A'CCwGG_T
Cuts at: 4099 4099
Size: 10813
SfcI C'TryA_G
Cuts at: 826 1045 2561 3533 3629 4011 4712 4739 4760
Size: 219 1516 972 96 382 701 27 21
Cuts at: 4760 4778 5021 5084 5378 5453 5906 5960 6023
Size: 18 243 63 294 75 453 54 63
Cuts at: 6023 6149 6275 6452 6917 7253 7469 7886 8161
Size: 126 126 177 465 336 216 417 275
Cuts at: 8161 9296 9977 10168 826
Size: 1135 681 191 1471
Fragments arranged by size:
1516 1471 1135 972 701 681 465 453
417 382 336 294 275 243 219 216
191 177 126 126 96 75 63 63
54 27 21 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 5867 5867
Size: 10813
SfoI GGC'GCC
Cuts at: 2043 2863 3439 5942 6314 6362 7388 2043
Size: 820 576 2503 372 48 1026 5468
Fragments arranged by size:
5468 2503 1026 820 576 372 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10813
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10813
SmaI CCC'GGG
Cuts at: 1872 2032 4571 5495 6188 6374 7181 7862 8140
Size: 160 2539 924 693 186 807 681 278
Cuts at: 8140 1872
Size: 4545
Fragments arranged by size:
4545 2539 924 807 693 681 278 186
160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2990 4658 5054 5526 6893 7223
Size: 197 926 1047 1668 396 472 1367 330
Cuts at: 7223 7634 8917 9818 10080 820
Size: 411 1283 901 262 1553
Fragments arranged by size:
1668 1553 1367 1283 1047 926 901 472
411 396 330 262 197
SnaBI TAC'GTA
Cuts at: 493 8604 493
Size: 8111 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10813
SphI G_CATG'C
Cuts at: 1132 2560 8171 1132
Size: 1428 5611 3774
Fragments arranged by size:
5611 3774 1428
SrfI GCCC'GGGC
Cuts at: 2032 5495 2032
Size: 3463 7350
SspI AAT'ATT
Cuts at: 5 52 8737 5
Size: 47 8685 2081
Fragments arranged by size:
8685 2081 47
StuI AGG'CCT
Cuts at: 1517 2360 2768 4415 1517
Size: 843 408 1647 7915
Fragments arranged by size:
7915 1647 843 408
StyI C'CwwG_G
Cuts at: 513 1065 2004 2913 3360 3414 3433 3979 4389
Size: 552 939 909 447 54 19 546 410
Cuts at: 4389 4695 5583 6177 6198 6477 7521 8252 513
Size: 306 888 594 21 279 1044 731 3074
Fragments arranged by size:
3074 1044 939 909 888 731 594 552
546 447 410 306 279 54 21 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 2353 2552 4390 7571 8840 8857
Size: 1255 584 189 199 1838 3181 1269 17
Cuts at: 8857 9010 9195 9640 325
Size: 153 185 445 1498
Fragments arranged by size:
3181 1838 1498 1269 1255 584 445 199
189 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6568 9059 10771
Size: 274 80 33 51 1443 4591 2491 1712
Cuts at: 10771 96
Size: 138
Fragments arranged by size:
4591 2491 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3234 3266 3683 3715 4970 5002 7692 7724 10773
Size: 32 417 32 1255 32 2690 32 3049
Cuts at: 10773 10805 3234
Size: 32 3242
Fragments arranged by size:
3242 3049 2690 1255 417 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2020 3890 1316
Size: 704 1870 8239
Fragments arranged by size:
8239 1870 704
XbaI T'CTAG_A
Cuts at: 8149 8149
Size: 10813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3127 3562 4396 6169 6481 6868 3127
Size: 435 834 1773 312 387 7072
Fragments arranged by size:
7072 1773 834 435 387 312
XhoI C'TCGA_G
Cuts at: 1943 2990 1943
Size: 1047 9766
XmaI C'CCGG_G
Cuts at: 1870 2030 4569 5493 6186 6372 7179 7860 8138
Size: 160 2539 924 693 186 807 681 278
Cuts at: 8138 1870
Size: 4545
Fragments arranged by size:
4545 2539 924 807 693 681 278 186
160
XmnI GAAnn'nnTTC
Cuts at: 3494 8942 3494
Size: 5448 5365
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6745 8621 276
Size: 53 83 186 936 5211 1876 2468
Fragments arranged by size:
5211 2468 1876 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FspAI NruI NsiI PacI PsrI SwaI