Restriction map for
pFN21AA2032
(Circular) (Six-base) MAPSORT of: pFN21AA2032.seq Check: 8549 from: 1 to: 7537
pFN21AA2032 7537 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2845 2845
Size: 7537
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5347 278
Size: 53 83 186 936 3811 2468
Fragments arranged by size:
3811 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4880 1937
Size: 2943 4594
Acc65I G'GTAC_C
Cuts at: 2671 3767 4858 2671
Size: 1096 1091 5350
Fragments arranged by size:
5350 1096 1091
AclI AA'CG_TT
Cuts at: 1563 5664 6037 1563
Size: 4101 373 3063
Fragments arranged by size:
4101 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3503 3525 4248 4764 5600 6984 7252 1632
Size: 1871 22 723 516 836 1384 268 1917
Fragments arranged by size:
1917 1871 1384 836 723 516 268 22
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7340
AflIII A'CryG_T
Cuts at: 1203 6436 7413 1203
Size: 5233 977 1327
Fragments arranged by size:
5233 1327 977
AgeI A'CCGG_T
Cuts at: 6368 6368
Size: 7537
AhdI GACnn_n'nnGTC
Cuts at: 6266 6266
Size: 7537
AleI CACnn'nnGTG
Cuts at: 1166 3713 1166
Size: 2547 4990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7537
AlwNI CAG_nnn'CTG
Cuts at: 2903 5280 6852 2903
Size: 2377 1572 3588
Fragments arranged by size:
3588 2377 1572
ApaLI G'TGCA_C
Cuts at: 5594 6750 5594
Size: 1156 6381
ApoI r'AATT_y
Cuts at: 1493 2299 2475 2547 3265 4846 5072 5224 7333
Size: 806 176 72 718 1581 226 152 2109
Cuts at: 7333 1493
Size: 1697
Fragments arranged by size:
2109 1697 1581 806 718 226 176 152
72
AseI AT'TA_AT
Cuts at: 160 1032 2880 4178 6091 160
Size: 872 1848 1298 1913 1606
Fragments arranged by size:
1913 1848 1606 1298 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7537
AvaI C'yCGr_G
Cuts at: 1870 1943 4423 4624 4862 1870
Size: 73 2480 201 238 4545
Fragments arranged by size:
4545 2480 238 201 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3751 3784 3751
Size: 33 7504
BamHI G'GATC_C
Cuts at: 4867 5234 4867
Size: 367 7170
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2671 3767 4435 4858 6313
Size: 325 217 627 884 1096 668 423 1455
Cuts at: 6313 618
Size: 1842
Fragments arranged by size:
1842 1455 1096 884 668 627 423 325
217
BanII G_rGCy'C
Cuts at: 1391 2154 3300 3837 4856 1391
Size: 763 1146 537 1019 4072
Fragments arranged by size:
4072 1146 1019 763 537
BbeI G_GCGC'C
Cuts at: 4439 4439
Size: 7537
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2461 2748 928
Size: 971 562 287 5717
Fragments arranged by size:
5717 971 562 287
BbvCI CC'TCA_GC
Cuts at: 2968 2985 3736 4519 2968
Size: 17 751 783 5986
Fragments arranged by size:
5986 783 751 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5728 5762 1903
Size: 34 3791 34 3678
Fragments arranged by size:
3791 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2090 4640 5429 6639 2090
Size: 2550 789 1210 2988
Fragments arranged by size:
2988 2550 1210 789
BclI T'GATC_A
Cuts at: 1548 3822 1548
Size: 2274 5263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6148 136
Size: 107 122 71 631 605 4476 1525
Fragments arranged by size:
4476 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3775 7532 3775
Size: 3757 3780
BlpI GC'TnA_GC
Cuts at: 4752 4954 4752
Size: 202 7335
Bme1580I G_kGCm'C
Cuts at: 1790 3951 5598 6754 1790
Size: 2161 1647 1156 2573
Fragments arranged by size:
2573 2161 1647 1156
BmgBI CAC'GTC
Cuts at: 1318 3711 1318
Size: 2393 5144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 2861 5268
Size: 416 53 83 399 24 732 712 2407
Cuts at: 5268 6226 442
Size: 958 1753
Fragments arranged by size:
2407 1753 958 732 712 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3585 3979 4482 6197 1239
Size: 2346 394 503 1715 2579
Fragments arranged by size:
2579 2346 1715 503 394
Bpu10I CC'TnA_GC
Cuts at: 2968 2985 3736 4519 2968
Size: 17 751 783 5986
Fragments arranged by size:
5986 783 751 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4021 4320 5662 6527 6825 4021
Size: 299 1342 865 298 4733
Fragments arranged by size:
4733 1342 865 299 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2968 3995 6200 882
Size: 617 1469 1027 2205 2219
Fragments arranged by size:
2219 2205 1469 1027 617
BsaAI yAC'GTr
Cuts at: 493 1204 5328 493
Size: 711 4124 2702
Fragments arranged by size:
4124 2702 711
BsaBI GATnn'nnATC
Cuts at: 7531 7531
Size: 7537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4436 5344 5726 6361
Size: 53 83 186 936 2903 908 382 635
Cuts at: 6361 275
Size: 1451
Fragments arranged by size:
2903 1451 936 908 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5970 6368 6642 6789 7200 5970
Size: 398 274 147 411 6307
Fragments arranged by size:
6307 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3055 3085 3583 3613 3640
Size: 30 165 30 1652 30 498 30 27
Cuts at: 3640 3670 1178
Size: 30 5045
Fragments arranged by size:
5045 1652 498 165 30 30 30 30
30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 2802 2956 3451 3724 1186
Size: 1202 414 154 495 273 4999
Fragments arranged by size:
4999 1202 495 414 273 154
BseYI C'CCAG_C
Cuts at: 2791 3371 4492 6740 7236 2791
Size: 580 1121 2248 496 3092
Fragments arranged by size:
3092 2248 1121 580 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2526 3021 3189 1163
Size: 573 790 495 168 5511
Fragments arranged by size:
5511 790 573 495 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4987 5748 6776 1540
Size: 275 186 2986 761 1028 2301
Fragments arranged by size:
2986 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5598 5683 6754 5598
Size: 85 1071 6381
Fragments arranged by size:
6381 1071 85
BsmI GAATG_Cn'
Cuts at: 4523 5048 5141 4523
Size: 525 93 6919
Fragments arranged by size:
6919 525 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3636 3636
Size: 7537
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 3300 3837 3951 4856 5598 5683
Size: 399 364 1146 537 114 905 742 85
Cuts at: 5683 6754 1391
Size: 1071 2174
Fragments arranged by size:
2174 1146 1071 905 742 537 399 364
114 85
BspEI T'CCGG_A
Cuts at: 7200 7200
Size: 7537
BspHI T'CATG_A
Cuts at: 5426 7156 5426
Size: 1730 5807
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 2845 2934 2950 4705 4825 4892 844
Size: 1810 191 89 16 1755 120 67 3489
Fragments arranged by size:
3489 1810 1755 191 120 89 67 16
BsrBI CCG'CTC
Cuts at: 1394 1823 4547 5424 1394
Size: 429 2724 877 3507
Fragments arranged by size:
3507 2724 877 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2172 3026 3665 4071 6032 6206 65
Size: 1183 924 854 639 406 1961 174 1396
Fragments arranged by size:
1961 1396 1183 924 854 639 406 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3380 4562 6181 6368 1700
Size: 110 1570 1182 1619 187 2869
Fragments arranged by size:
2869 1619 1570 1182 187 110
BsrGI T'GTAC_A
Cuts at: 96 3673 96
Size: 3577 3960
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7537
BssSI C'ACGA_G
Cuts at: 5597 6609 5597
Size: 1012 6525
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2639 2648 794
Size: 1845 9 5683
Fragments arranged by size:
5683 1845 9
BstBI TT'CG_AA
Cuts at: 2750 6341 2750
Size: 3591 3946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7537
BstYI r'GATC_y
Cuts at: 1972 3775 4867 5234 5619 5636 7088 7174 7186
Size: 1803 1092 367 385 17 1452 86 12
Cuts at: 7186 7532 1972
Size: 346 1977
Fragments arranged by size:
1977 1803 1452 1092 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3161 3636 513
Size: 552 939 1157 475 4414
Fragments arranged by size:
4414 1157 939 552 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6798
Fragments arranged by size:
6798 683 56
BtsI GCAGTG_nn'
Cuts at: 3033 3104 3690 4086 4305 5053 5847 5867 3033
Size: 71 586 396 219 748 794 20 4703
Fragments arranged by size:
4703 794 748 586 396 219 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7537
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2193 2228 4054 4089 529
Size: 35 1629 35 1826 35 3977
Fragments arranged by size:
3977 1826 1629 35 35 35
DraI TTT'AAA
Cuts at: 4014 4841 4014
Size: 827 6710
DraIII CAC_nnn'GTG
Cuts at: 2648 3392 2648
Size: 744 6793
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6544 809
Size: 507 5228 1802
Fragments arranged by size:
5228 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4984 5873 7502
Size: 54 1425 50 275 18 3154 889 1629
Cuts at: 7502 8
Size: 43
Fragments arranged by size:
3154 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4984 1537
Size: 275 3172 4090
Fragments arranged by size:
4090 3172 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3244 3522 3538 3816 4098 4416 5467 1353
Size: 1891 278 16 278 282 318 1051 3423
Fragments arranged by size:
3423 1891 1051 318 282 278 278 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6124 6498 6644 387
Size: 1064 138 4535 374 146 1280
Fragments arranged by size:
4535 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3503 3525 3585 3979 4248 4482 4764
Size: 393 1871 22 60 394 269 234 282
Cuts at: 4764 5600 6197 6984 7252 1239
Size: 836 597 787 268 1524
Fragments arranged by size:
1871 1524 836 787 597 394 393 282
269 268 234 60 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3147 3147
Size: 7537
EcoO109I rG'GnC_Cy
Cuts at: 3113 3152 4340 3113
Size: 39 1188 6310
Fragments arranged by size:
6310 1188 39
EcoRI G'AATT_C
Cuts at: 2299 2547 4846 5224 2299
Size: 248 2299 378 4612
Fragments arranged by size:
4612 2299 378 248
EcoRV GAT'ATC
Cuts at: 2412 2412
Size: 7537
FspI TGC'GCA
Cuts at: 4695 6043 4695
Size: 1348 6189
HaeII r_GCGC'y
Cuts at: 1678 4439 4787 6684 1678
Size: 2761 348 1897 2531
Fragments arranged by size:
2761 2531 1897 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 3583 3615 6178
Size: 32 135 32 428 32 1551 32 2563
Cuts at: 6178 6210 6252 6284 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2563 1551 428 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2801 4881 5127 669
Size: 988 281 863 2080 246 3079
Fragments arranged by size:
3079 2080 988 863 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7537
HpaI GTT'AAC
Cuts at: 5127 5127
Size: 7537
KasI G'GCGC_C
Cuts at: 4435 4435
Size: 7537
KpnI G_GTAC'C
Cuts at: 2675 3771 4862 2675
Size: 1096 1091 5350
Fragments arranged by size:
5350 1096 1091
MfeI C'AATT_G
Cuts at: 2079 5136 2079
Size: 3057 4480
MluI A'CGCG_T
Cuts at: 7413 7413
Size: 7537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3360 6651 6835 1305
Size: 2055 3291 184 2007
Fragments arranged by size:
3291 2055 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5715
Fragments arranged by size:
5715 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 5495 5854 6013 518
Size: 648 2547 1782 359 159 2042
Fragments arranged by size:
2547 2042 1782 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2903 4199 4241 4454 4953 5630 6778 7023
Size: 1297 1296 42 213 499 677 1148 245
Cuts at: 7023 1606
Size: 2120
Fragments arranged by size:
2120 1297 1296 1148 677 499 245 213
42
NarI GG'CG_CC
Cuts at: 4436 4436
Size: 7537
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6046
Fragments arranged by size:
6046 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7537
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7537
NotI GC'GGCC_GC
Cuts at: 4984 4984
Size: 7537
NspI r_CATG'y
Cuts at: 1132 1229 2633 4895 6440 1132
Size: 97 1404 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 1404 97
PasI CC'CwG_GG
Cuts at: 1348 2954 4169 4634 1348
Size: 1606 1215 465 4251
Fragments arranged by size:
4251 1606 1215 465
PciI A'CATG_T
Cuts at: 6436 6436
Size: 7537
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4055 1108
Size: 2947 4590
PmeI GTTT'AAAC
Cuts at: 4841 4841
Size: 7537
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5606 5638 7119 7151 3515
Size: 32 2059 32 1481 32 3901
Fragments arranged by size:
3901 2059 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3113 3152 4340 3113
Size: 39 1188 6310
Fragments arranged by size:
6310 1188 39
PshAI GACnn'nnGTC
Cuts at: 1613 4345 1613
Size: 2732 4805
PsiI TTA'TAA
Cuts at: 2655 5107 2655
Size: 2452 5085
PstI C_TGCA'G
Cuts at: 830 4434 4591 4889 830
Size: 3604 157 298 3478
Fragments arranged by size:
3604 3478 298 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7537
PvuII CAG'CTG
Cuts at: 2903 4241 4454 2903
Size: 1338 213 5986
Fragments arranged by size:
5986 1338 213
SalI G'TCGA_C
Cuts at: 1936 4879 1936
Size: 2943 4594
SapI GCTCTTCn'nnn_
Cuts at: 3244 4098 4416 3244
Size: 854 318 6365
Fragments arranged by size:
6365 854 318
SbfI CC_TGCA'GG
Cuts at: 4889 4889
Size: 7537
ScaI AGT'ACT
Cuts at: 4006 5785 7497 4006
Size: 1779 1712 4046
Fragments arranged by size:
4046 1779 1712
SfcI C'TryA_G
Cuts at: 826 1045 2724 4430 4443 4587 4885 6020 6701
Size: 219 1679 1706 13 144 298 1135 681
Cuts at: 6701 6892 826
Size: 191 1471
Fragments arranged by size:
1706 1679 1471 1135 681 298 219 191
144 13
SfoI GGC'GCC
Cuts at: 4437 4437
Size: 7537
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7537
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7537
SmaI CCC'GGG
Cuts at: 1872 4425 4626 4864 1872
Size: 2553 201 238 4545
Fragments arranged by size:
4545 2553 238 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4000 4335 5641 6542 6804 820
Size: 197 926 2057 335 1306 901 262 1553
Fragments arranged by size:
2057 1553 1306 926 901 335 262 197
SnaBI TAC'GTA
Cuts at: 493 5328 493
Size: 4835 2702
SpeI A'CTAG_T
Cuts at: 152 2254 152
Size: 2102 5435
SphI G_CATG'C
Cuts at: 1132 4895 1132
Size: 3763 3774
SspI AAT'ATT
Cuts at: 5 52 5461 5
Size: 47 5409 2081
Fragments arranged by size:
5409 2081 47
StuI AGG'CCT
Cuts at: 1517 3330 3624 4611 1517
Size: 1813 294 987 4443
Fragments arranged by size:
4443 1813 987 294
StyI C'CwwG_G
Cuts at: 513 1065 2004 3325 4976 513
Size: 552 939 1321 1651 3074
Fragments arranged by size:
3074 1651 1321 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3777 4818 5564 5581 5734 5919 6364
Size: 1255 2197 1041 746 17 153 185 445
Cuts at: 6364 325
Size: 1498
Fragments arranged by size:
2197 1498 1255 1041 746 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2516 3059 3673
Size: 274 80 33 51 1443 539 543 614
Cuts at: 3673 4004 5783 7495 96
Size: 331 1779 1712 138
Fragments arranged by size:
1779 1712 1443 614 543 539 331 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3061 3093 4078 4110 4297 4329 7497 7529 3061
Size: 32 985 32 187 32 3168 32 3069
Fragments arranged by size:
3168 3069 985 187 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7537
XbaI T'CTAG_A
Cuts at: 4873 4873
Size: 7537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3604 3604
Size: 7537
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7537
XmaI C'CCGG_G
Cuts at: 1870 4423 4624 4862 1870
Size: 2553 201 238 4545
Fragments arranged by size:
4545 2553 238 201
XmnI GAAnn'nnTTC
Cuts at: 3022 5666 3022
Size: 2644 4893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5345 276
Size: 53 83 186 936 3811 2468
Fragments arranged by size:
3811 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI